LOCUS       sequence-378002-       10020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains stuffer sequence into which sgRNAs can be cloned and 
            expresses mTurquoise2 from hepatocyte-specific promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Keys HR, Knouse KA
  TITLE     Genome-scale CRISPR screening in a single mouse liver.
  JOURNAL   Cell Genom. 2022 Dec 14;2(12):100217. doi: 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15.
  PUBMED    36643909
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-12-14"; volume: "2(12):100217. doi"; pages: " 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15"
COMMENT     Sequence Label: pLentiCRISPRv2-Stuffer-HepmTurquoise2
FEATURES             Location/Qualifiers
     source          1..10020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      486..495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(492..494,495..497,498..1211)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(537..558)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(798..817)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1145..1166
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1230..1818
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1283..1303)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1701..1712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1890..2123
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2149..2166)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2155..2379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2425..2853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2512..2531)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2722..2743
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2862..2882)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2867..3196
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3047..3182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3109..3128
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3244..3291
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3310..3684
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3814..3935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3851..3870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3905..3924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3984..4000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3984..4000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3997..4019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4008..4024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4032..4038,4039..4056,4057..4062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4077..4098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4215..4232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4386..4974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4466..4485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5145..5936,5937..6005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5768..5787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6006..6110)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6185..6204)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6376..6755
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6757..6955
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6910..6930
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6973..7153
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7200..7325
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7818..8051
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8236..8280
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8578..8695
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8746..8994
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8746..8766
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8917..8936
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ttatacattc catcgatgta gttcttgctc ttttttgtac gtctctgttt aagagctatg
       61 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
      121 ccgagtcggt gcttttttga attcgctagc accggcgcgc cgggggaggc tgctggtgaa
      181 tattaaccaa ggtcacccca gttatcggag gagcaaacag gggctaagtc cacacgcgtg
      241 gtaccgtctg tctgcacatt tcgtagagcg agtgttccga tactctaatc tccctaggca
      301 aggttcatat ttgtgtaggt tacttattct ccttttgttg actaagtcaa taatcagaat
      361 cagcaggttt ggagtcagct tggcagggat cagcagcctg ggttggaagg agggggtata
      421 aaagcccctt caccaggaga agccgtcaca cagatccaca agctcctgac cggttctaga
      481 gcgctgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      541 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      601 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      661 cctggcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg
      721 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      781 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      841 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      901 tcctggggca caagctggag tacaactact ttagcgacaa cgtctatatc accgccgaca
      961 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1021 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1081 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     1141 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1201 tgtacaagta atgaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     1261 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1321 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     1381 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     1441 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     1501 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     1561 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     1621 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     1681 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     1741 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     1801 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     1861 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     1921 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     1981 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     2041 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     2101 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     2161 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2221 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2281 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2341 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2401 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2461 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2521 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2581 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2641 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2701 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2761 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2821 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     2881 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2941 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3001 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3061 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3121 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3181 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     3241 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     3301 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     3361 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     3421 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     3481 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     3541 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     3601 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     3661 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     3721 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     3781 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     3841 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3901 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     3961 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4021 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4081 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4141 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4201 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4261 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6121 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     6181 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     6241 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     6301 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     6361 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     6421 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     6481 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     6541 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     6601 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     6661 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     6721 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     6781 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     6841 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     6901 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
     6961 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7021 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7081 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7141 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     7201 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     7261 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     7321 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     7381 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     7441 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     7501 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     7561 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     7621 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     7681 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     7741 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     7801 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     7861 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     7921 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     7981 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     8041 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     8101 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     8161 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     8221 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     8281 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     8341 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     8401 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     8461 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     8521 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     8581 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     8641 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     8701 ttattacagg gacagcagag atccagtttg gttaattaag gtaccgaggg cctatttccc
     8761 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     8821 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     8881 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     8941 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggagac
     9001 ggttgtaaat gagcacacaa aatacacatg ctaaaatatt atattctatg acctttataa
     9061 aatcaaccaa aatcttcttt ttaataactt tagtatcaat aattagaatt tttatgttcc
     9121 tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa cgctagtttt agtaactcgc
     9181 gttgttttct tcacctttaa taatagctac tccaccactt gttcctaagc ggtcagctcc
     9241 tgcttcaatc attttttgag catcttcaaa tgttctaact ccaccagctg ctttaactaa
     9301 agcattgtct ttaacaactg acttcattag tttaacatct tcaaatgttg cacctgattt
     9361 tgaaaatcct gttgatgttt taacaaattc taatccagct tcaacagcta tttcacaagc
     9421 tttcatgatt tcttcttttg ttaataaaca attttccata atacatttaa caacatgtga
     9481 tccagctgct ttttttacag ctttcatgtc ttctaaaact aattcataat ttttgtcttt
     9541 taatgcacca atatttaata ccatatcaat ttctgttgca ccatctttaa ttgcttcaga
     9601 aacttcgaat gcttttgtag ctgttgtgca tgcacctaga ggaaaaccta caacatttgt
     9661 tattcctaca tttgtgcctt ttaataattc tttacaatag cttgttcaat atgaattaac
     9721 acaaactgtt gcaaaatcaa attcaattgc ttcatcacat aattgtttaa tttcagcttt
     9781 cgtagcatct tgttttaata atgtgtgatc tatatatttg tttagtttca ttttttctcc
     9841 tatatattca tttttaattt taattcttta ataatttcgt ctactttaac tttagcgttt
     9901 tgaacagatt caccaacacc tataaaataa atttttagtt taggttcagt tccacttggg
     9961 cgaacagcaa atcatgactt atcttctaaa taaaatttta gtaagtcttg tcctggcata
//
