LOCUS       sequence-378003-        8959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for 
            mouse) and mCherry from hepatocyte-specific promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Keys HR, Knouse KA
  TITLE     Genome-scale CRISPR screening in a single mouse liver.
  JOURNAL   Cell Genom. 2022 Dec 14;2(12):100217. doi: 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15.
  PUBMED    36643909
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-12-14"; volume: "2(12):100217. doi"; pages: " 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15"
COMMENT     Sequence Label: pLentiCRISPRv2-sgAAVS1-HepmCherry
FEATURES             Location/Qualifiers
     source          1..8959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..125)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        297..676
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        678..876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     831..851
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             894..1074
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1121..1246
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1739..1972
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2157..2201
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2499..2616
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2667..2915
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2667..2687
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2838..2857
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     regulatory      3375..3384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3381..4091
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3526..3544)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3794..3813
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4110..4698
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4163..4183)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4581..4592)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4770..5003
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5029..5046)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5035..5259
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5305..5733
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5392..5411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5602..5623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5742..5762)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5747..6076
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5927..6062
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5989..6008
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6124..6171
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6190..6564
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6694..6815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6731..6750)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6785..6804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6864..6880)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6864..6880)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6877..6899)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6888..6904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6912..6918,6919..6936,6937..6942))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6957..6978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7095..7112)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7266..7854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7346..7365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8025..8816,8817..8885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8648..8667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8886..8959,1..31))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
       61 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      121 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      181 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      241 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      301 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      361 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      421 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      481 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      541 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      601 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      661 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      721 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      781 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      841 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      901 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      961 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     1021 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1081 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1141 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1201 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1261 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1321 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1381 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1441 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1501 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1561 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1621 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1681 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1741 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1801 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1861 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1921 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1981 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     2041 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2101 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2161 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2221 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2281 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2341 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2401 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2461 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2521 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2581 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2641 gatccagttt ggttaattaa ggtaccgagg gcctatttcc catgattcct tcatatttgc
     2701 atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga
     2761 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     2821 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     2881 tggctttata tatcttgtgg aaaggacgaa acaccggggc cactagggac aggatgttta
     2941 agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa
     3001 aaagtggcac cgagtcggtg cttttttgaa ttcgctagca ccggcgcgcc gggggaggct
     3061 gctggtgaat attaaccaag gtcaccccag ttatcggagg agcaaacagg ggctaagtcc
     3121 acacgcgtgg taccgtctgt ctgcacattt cgtagagcga gtgttccgat actctaatct
     3181 ccctaggcaa ggttcatatt tgtgtaggtt acttattctc cttttgttga ctaagtcaat
     3241 aatcagaatc agcaggtttg gagtcagctt ggcagggatc agcagcctgg gttggaagga
     3301 gggggtataa aagccccttc accaggagaa gccgtcacac agatccacaa gctcctgacc
     3361 ggttctagag cgctgccacc atggtgagca agggcgagga ggataacatg gccatcatca
     3421 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     3481 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     3541 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     3601 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     3661 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     3721 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     3781 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     3841 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     3901 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     3961 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     4021 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     4081 tgtacaagta atgaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     4141 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4201 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4261 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4321 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4381 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4441 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4501 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4561 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4621 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4681 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     4741 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     4801 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4861 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4921 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     4981 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     5041 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5101 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5161 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5221 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     5281 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5341 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5401 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5461 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5521 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5581 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5641 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5701 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5821 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5881 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5941 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6001 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6061 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6121 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     6181 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     6241 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     6301 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     6361 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     6421 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     6481 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     6541 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     6601 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6661 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6721 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6781 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6841 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6901 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6961 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7021 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7081 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7141 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8341 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8641 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8941 ggatacatat ttgaatgta
//
