LOCUS       sequence-378004-        8968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for 
            mouse) and mTurquoise2 from hepatocyte-specific promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Keys HR, Knouse KA
  TITLE     Genome-scale CRISPR screening in a single mouse liver.
  JOURNAL   Cell Genom. 2022 Dec 14;2(12):100217. doi: 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15.
  PUBMED    36643909
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-12-14"; volume: "2(12):100217. doi"; pages: " 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15"
COMMENT     Sequence Label: pLentiCRISPRv2-sgAAVS1-HepmTurquoise2
FEATURES             Location/Qualifiers
     source          1..8968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(157..176)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        348..727
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        729..927
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     882..902
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             945..1125
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1172..1297
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1790..2023
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2208..2252
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2550..2667
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2718..2966
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2718..2738
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2889..2908
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     regulatory      3426..3435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3432..3434,3435..3437,3438..4151)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3477..3498)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3738..3757)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4085..4106
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4170..4758
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4223..4243)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4641..4652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4830..5063
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5089..5106)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5095..5319
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5365..5793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5452..5471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5662..5683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5802..5822)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5807..6136
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5987..6122
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6049..6068
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6184..6231
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6250..6624
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6754..6875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6791..6810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6845..6864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6924..6940)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6924..6940)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6937..6959)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6948..6964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6972..6978,6979..6996,6997..7002))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7017..7038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7155..7172)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7326..7914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7406..7425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8085..8876,8877..8945))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8708..8727
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8946..8968,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
       61 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
      121 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
      181 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      241 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      301 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      361 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      421 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      481 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      541 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      601 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      661 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      721 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      781 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      841 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      901 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      961 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     1021 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     1081 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     1141 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     1201 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     1261 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1321 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1381 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1441 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1501 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1561 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1621 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1681 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1741 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1801 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1861 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1921 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1981 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     2041 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     2101 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     2161 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     2221 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     2281 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     2341 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     2401 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2461 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2521 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2581 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2641 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2701 tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat
     2761 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
     2821 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     2881 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     2941 atatcttgtg gaaaggacga aacaccgggg ccactaggga caggatgttt aagagctatg
     3001 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
     3061 ccgagtcggt gcttttttga attcgctagc accggcgcgc cgggggaggc tgctggtgaa
     3121 tattaaccaa ggtcacccca gttatcggag gagcaaacag gggctaagtc cacacgcgtg
     3181 gtaccgtctg tctgcacatt tcgtagagcg agtgttccga tactctaatc tccctaggca
     3241 aggttcatat ttgtgtaggt tacttattct ccttttgttg actaagtcaa taatcagaat
     3301 cagcaggttt ggagtcagct tggcagggat cagcagcctg ggttggaagg agggggtata
     3361 aaagcccctt caccaggaga agccgtcaca cagatccaca agctcctgac cggttctaga
     3421 gcgctgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3481 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3541 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3601 cctggcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg
     3661 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3721 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3781 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3841 tcctggggca caagctggag tacaactact ttagcgacaa cgtctatatc accgccgaca
     3901 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     3961 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4021 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     4081 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4141 tgtacaagta atgaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     4201 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4261 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4321 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4381 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4441 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4501 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4561 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4621 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4681 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4741 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     4801 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     4861 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4921 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4981 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5041 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     5101 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5161 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5221 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5281 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     5341 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5581 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5701 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5761 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5821 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5881 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5941 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6061 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6121 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6181 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     6241 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     6301 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     6361 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     6421 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     6481 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     6541 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     6601 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     6661 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6721 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6781 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6841 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6901 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6961 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7021 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7081 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7141 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7201 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7261 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7321 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7381 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7441 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7501 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7561 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7621 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7681 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7741 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7801 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7861 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7921 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7981 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8041 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8101 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8161 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8221 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8281 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8341 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8401 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8461 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8521 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8581 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8641 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8701 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8761 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8821 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8881 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8941 ctcatactct tcctttttca atattatt
//
