LOCUS       sequence-378006-        8960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Keys HR, Knouse KA
  TITLE     Genome-scale CRISPR screening in a single mouse liver.
  JOURNAL   Cell Genom. 2022 Dec 14;2(12):100217. doi: 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15.
  PUBMED    36643909
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-12-14"; volume: "2(12):100217. doi"; pages: " 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15"
COMMENT     Sequence Label: pLentiCRISPRv2-sgMaob-HepmCherry
FEATURES             Location/Qualifiers
     source          1..8960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        294..673
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        675..873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     828..848
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             891..1071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1118..1243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1736..1969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2154..2198
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2496..2613
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2664..2912
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2664..2684
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2835..2854
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     regulatory      3373..3382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3379..4089
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3524..3542)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3792..3811
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4108..4696
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4161..4181)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4579..4590)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4768..5001
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5027..5044)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5033..5257
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5303..5731
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5390..5409)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5600..5621
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5740..5760)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5745..6074
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5925..6060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5987..6006
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6122..6169
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6188..6562
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6692..6813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6729..6748)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6783..6802
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6862..6878)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6862..6878)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6875..6897)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6886..6902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6910..6916,6917..6934,6935..6940))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6955..6976
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7093..7110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7264..7852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7344..7363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8023..8814,8815..8883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8646..8665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8884..8960,1..28))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
       61 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
      121 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      181 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      241 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      301 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      361 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      421 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      481 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      541 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      601 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      661 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      721 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      781 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      841 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
      901 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      961 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1021 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1081 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1141 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1201 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1261 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1321 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1381 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1441 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1501 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1561 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     1621 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1681 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1741 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1801 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1861 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1921 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1981 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2041 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2101 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2161 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2221 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2281 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2341 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2401 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     2461 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     2521 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2581 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     2641 ccagtttggt taattaaggt accgagggcc tatttcccat gattccttca tatttgcata
     2701 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
     2761 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     2821 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     2881 ctttatatat cttgtggaaa ggacgaaaca ccgacggata aaggatatac ttggtttaag
     2941 agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa
     3001 agtggcaccg agtcggtgct tttttgaatt cgctagcacc ggcgcgccgg gggaggctgc
     3061 tggtgaatat taaccaaggt caccccagtt atcggaggag caaacagggg ctaagtccac
     3121 acgcgtggta ccgtctgtct gcacatttcg tagagcgagt gttccgatac tctaatctcc
     3181 ctaggcaagg ttcatatttg tgtaggttac ttattctcct tttgttgact aagtcaataa
     3241 tcagaatcag caggtttgga gtcagcttgg cagggatcag cagcctgggt tggaaggagg
     3301 gggtataaaa gccccttcac caggagaagc cgtcacacag atccacaagc tcctgaccgg
     3361 ttctagagcg ctgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     3421 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     3481 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     3541 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     3601 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     3661 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     3721 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     3781 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     3841 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     3901 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     3961 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     4021 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     4081 tacaagtaat gaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     4141 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4201 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4261 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4321 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4381 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4441 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4501 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4561 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4621 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4681 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     4741 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     4801 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4861 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4921 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4981 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     5041 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5101 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5161 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5221 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5281 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5341 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5401 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5461 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5521 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5581 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5641 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5701 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     5761 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5821 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5881 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5941 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6001 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6061 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     6121 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     6181 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     6241 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     6301 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     6361 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     6421 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     6481 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     6541 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     6601 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6661 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6721 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6781 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6841 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6901 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6961 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7021 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7081 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8161 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8461 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8641 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8701 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8761 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8821 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8881 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8941 atacatattt gaatgtattt
//
