LOCUS       sequence-378007-        8960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Keys HR, Knouse KA
  TITLE     Genome-scale CRISPR screening in a single mouse liver.
  JOURNAL   Cell Genom. 2022 Dec 14;2(12):100217. doi: 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15.
  PUBMED    36643909
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-12-14"; volume: "2(12):100217. doi"; pages: " 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15"
COMMENT     Sequence Label: pLentiCRISPRv2-sgLmnb2-HepmCherry
FEATURES             Location/Qualifiers
     source          1..8960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..47)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(201..789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(281..300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(960..1751,1752..1820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1583..1602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1821..1925)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2000..2019)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2191..2570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2572..2770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2725..2745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2788..2968
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3015..3140
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3633..3866
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4051..4095
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4393..4510
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4561..4809
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4561..4581
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4732..4751
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     regulatory      5270..5279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5276..5986
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5421..5439)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5689..5708
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6005..6593
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6058..6078)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6476..6487)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6665..6898
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6924..6941)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6930..7154
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7200..7628
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7287..7306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7497..7518
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7637..7657)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7642..7971
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7822..7957
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7884..7903
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8019..8066
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8085..8459
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8589..8710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8626..8645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8680..8699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8759..8775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8759..8775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8772..8794)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8783..8799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8807..8813,8814..8831,8832..8837))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8852..8873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
       61 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1921 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     1981 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     2041 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     2101 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     2161 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     2221 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     2281 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2341 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2401 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2461 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2521 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2581 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2641 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2701 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2761 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     2821 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     2881 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     2941 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     3001 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     3061 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     3121 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     3181 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     3241 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     3301 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     3361 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     3421 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     3481 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     3541 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     3601 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     3661 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     3721 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     3781 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     3841 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     3901 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     3961 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     4021 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     4081 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     4141 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     4201 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     4261 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     4321 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
     4381 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     4441 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     4501 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttaaggtacc
     4561 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     4621 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     4681 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     4741 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     4801 cgaaacaccg aggtacggga gacccgacgg gtttaagagc tatgctggaa acagcatagc
     4861 aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     4921 ttgaattcgc tagcaccggc gcgccggggg aggctgctgg tgaatattaa ccaaggtcac
     4981 cccagttatc ggaggagcaa acaggggcta agtccacacg cgtggtaccg tctgtctgca
     5041 catttcgtag agcgagtgtt ccgatactct aatctcccta ggcaaggttc atatttgtgt
     5101 aggttactta ttctcctttt gttgactaag tcaataatca gaatcagcag gtttggagtc
     5161 agcttggcag ggatcagcag cctgggttgg aaggaggggg tataaaagcc ccttcaccag
     5221 gagaagccgt cacacagatc cacaagctcc tgaccggttc tagagcgctg ccaccatggt
     5281 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     5341 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     5401 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     5461 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     5521 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     5581 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     5641 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     5701 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     5761 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     5821 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     5881 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     5941 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaatgaa cgcgttaagt
     6001 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6061 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6121 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6181 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6241 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6301 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6361 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     6421 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6481 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6541 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     6601 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6661 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6721 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6781 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6841 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     6901 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     6961 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7021 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7081 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7141 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     7201 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7261 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7321 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7381 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     7441 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7501 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7561 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7621 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     7681 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     7741 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7801 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7861 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     7921 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     7981 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     8041 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     8101 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     8161 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     8221 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     8281 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     8341 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     8401 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     8461 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     8521 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8581 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8641 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8701 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     8761 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8821 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8881 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8941 gccaacgcgc ggggagaggc
//
