LOCUS       sequence-378008-       13551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test if a single plasmid stably transformed into Arabidopsis can 
            switched from off to on when heat shocked..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13551)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 13551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP792_L2_FRT-OCS-tGFP_Red_mCherry_HSP-FlpO
FEATURES             Location/Qualifiers
     source          1..13551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1587..1620
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(2345..2362)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     polyA_signal    2633..2807
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             4010..4705
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     CDS             5536..6243
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5681..5699)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      6494..6694
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             7576..7596
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8280..8468
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    9092..9266
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9329..9353
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9441..10151
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10172..10276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10277..11071
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11242..11830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11731..11750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12166..13314
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13460..13484
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta taggaacttc
     1621 caacaaacaa cattacaatt actatttaca attacaatga gaggatctgg atctgagtct
     1681 gatgagtctg gacttcctgc tatggaaatc gagtgtagaa tcactggaac ccttaacggt
     1741 gttgagttcg agcttgttgg aggtggtgag ggaactcctg agcagggaag aatgactaac
     1801 aagatgaagt ctaccaaggg tgctcttacc ttctctccat accttctttc tcacgttatg
     1861 ggatacggat tctaccactt cggaacttac ccatctggat acgagaaccc tttccttcat
     1921 gctatcaaca acggtggata caccaacact aggatcgaga agtacgagga tggtggtgtt
     1981 cttcacgtta gcttctctta cagatacgag gctggaagag tgatcggaga tttcaaggtt
     2041 atgggaactg gattccctga ggattctgtt atcttcaccg acaagatcat caggtctaac
     2101 gctactgttg agcatcttca tcctatggga gataacgatc tcgatggatc tttcaccaga
     2161 accttctcac ttagagatgg tggttactac tcttctgtgg tggattctca catgcacttc
     2221 aagtctgcta tccacccttc tatccttcaa aacggtggac ctatgttcgc tttcagaaga
     2281 gttgaggaag atcactctaa caccgagctt ggaatcgttg agtaccaaca tgctttcaag
     2341 acccctgatg ctgatgctgg tgaggaattc gaagagcaag caaggaaagc taaggtgaac
     2401 aatgagaaaa agacggaaat agtgaaacca gagagttgta gcaatgaagg agatgtcaag
     2461 gatctgaaaa gaaaggactc tgaggatgga aacgagggtg aggaagaaga agcttcttcg
     2521 aaaccgaaaa agccaaaagt tgctctttct catcttcagg acattgacga cacagaagct
     2581 gatcaagaag aagagtaagc ttctctagct agagtcgatc gacaagctcg agtttctcca
     2641 taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat
     2701 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     2761 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgct gcaagaattc
     2821 aagcttggag ttctagaatg tcgcggaaca aattttaaaa ctaaatccta aatttttcta
     2881 attttgttgc caatagtgga tatgtgggcc gtatagaagg aatctattga aggcccaaac
     2941 ccatactgac gagcccaaag gttcgttttg cgttttatgt ttcggttcga tgccaacgcc
     3001 acattctgag ctaggcaaaa aacaaacgtg tctttgaata gactcctctc gttaacacat
     3061 gcagcggctg catggtgacg ccattaacac gtggcctaca attgcatgat gtctccattg
     3121 acacgtgact tctcgtctcc tttcttaata tatctaacaa acactcctac ctcttccaaa
     3181 atatatacac atctttttga tcaatctctc attcaaaatc tcattctctc tagtaaacaa
     3241 gaacaaaaaa atggcggata cagctagagg aacccatcac gatatcatcg gcagagatca
     3301 gtacccgatg atgggccgag atcgtgacca gtaccagatg tccggacgag gatctgacta
     3361 ctccaagtct aggcagattg ctaaagctgc aactgctgtc acagctggtg gttccctcct
     3421 tgttctctcc agccttaccc ttgttggaac tgtcatagct ttgactgttg caacacctct
     3481 gctcgttatc ttcagcccaa tccttgtccc ggctctcatc acagttgcac tcctcatcac
     3541 cggttttctt tcctctggag ggtttggcat tgccgctata accgttttct cttggattta
     3601 caagtaagca cacatttatc atcttacttc ataattttgt gcaatatgtg catgcatgtg
     3661 ttgagccagt agctttggat caattttttt ggtcgaataa caaatgtaac aataagaaat
     3721 tgcaaattct agggaacatt tggttaacta aatacgaaat ttgacctagc tagcttgaat
     3781 gtgtctgtgt atatcatcta tataggtaaa atgcttggta tgatacctat tgattgtgaa
     3841 taggtacgca acgggagagc acccacaggg atcagacaag ttggacagtg caaggatgaa
     3901 gttgggaagc aaagctcagg atctgaaaga cagagctcag tactacggac agcaacatac
     3961 tggtggggaa catgaccgtg accgtactcg tggtggccag cacactacta tgagcgagct
     4021 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     4081 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     4141 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     4201 cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
     4261 ccctgagggc ttcacatggg agagagtcac cacatacgaa gatgggggcg tgctgaccgc
     4321 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     4381 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac
     4441 cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg ccctgaagct
     4501 cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc
     4561 taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga
     4621 ggccgacaaa gaaacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     4681 ccctagcaaa ctggggcaca agtgagctta ccccactgat gtcatcgtca tagtccaata
     4741 actccaatgt cggggagtta gtttatgagg aataaagtgt ttagaatttg atcaggggga
     4801 gataataaaa gccgagtttg aatctttttg ttataagtaa tgtttatgtg tgtttctata
     4861 tgttgtcaaa tggtaccatg ttttttttcc tctctttttg taacttgcaa gtgttgtgtt
     4921 gtactttatt tggcttcttt gtaagttggt aacggtggtc tatatatgga aaaggtcttg
     4981 ttttgttaaa cttatgttag ttaactggat tcgtctttaa ccacaaaaag ttttcaataa
     5041 gctacaaatt tagacacgca agccgatgca gtcattagta catatattta ttgcaagtga
     5101 ttacatggca acccaaactt caaaaacagt aggttgctcc atttagtcgc tactagaatt
     5161 cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct cattaagcgt
     5221 cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata gaaaattgga
     5281 aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg aaaaccctac
     5341 gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc cctcgtcgct
     5401 gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag agagaaaaca
     5461 atcaataagc gacctactac aaacaagtaa gtaagagaaa aaccaaaaga agaagagaaa
     5521 caacaagaag aagtaatggt gagcaagggc gaggaggata acatggccat catcaaggag
     5581 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     5641 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     5701 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     5761 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     5821 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     5881 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     5941 tccgacggcc ccgtaatgca gaagaaaacc atgggctggg aggcctcctc cgagcggatg
     6001 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     6061 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgcca
     6121 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     6181 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     6241 aagttcgaag agcaagcaag gaaagctaag gtgaacaatg agaaaaagac ggaaatagtg
     6301 aaaccagaga gttgtagcaa tgaaggagat gtcaaggatc tgaaaagaaa ggactctgag
     6361 gatggaaacg agggtgagga agaagaagct tcttcgaaac cgaaaaagcc aaaagttgct
     6421 ctttctcatc ttcaggacat tgacgacaca gaagctgatc aagaagaaga gtaagcttga
     6481 tcccccgtcg acagctagct atatcatcaa tttatgtatt acacataata tcgcactcag
     6541 tctttcatct acggcaatgt accagctgat ataatcagtt attgaaatat ttctgaattt
     6601 aaacttgcat caataaattt atgtttttgc ttggactata atacctgact tgttatttta
     6661 tcaataaata tttaaactat atttctttca agatcgcttt acgaattccc atggggagaa
     6721 gcttttctct tcatttctct ttctttcttt ctttcatgca agttctattg attagaaacc
     6781 aaaaccatga catatcacat tttcaatttt taaccccaag tttcccaaaa aggtaatcac
     6841 aattactttc catggtcatt tcttctggtt caagcatgac atgaacaggc aataaataag
     6901 ttgagatttt gatcacagta actgatactt gaatcgaatc atttagattt tttttttttt
     6961 tagtttactt gtttagtaaa tatgttgtct atgtttgtca caaaaacgtg gctcagttct
     7021 tgtatatatg gagacaaaaa aatccattaa aagattgttg acattctcgg aaatttagtg
     7081 ccaactgtta ttgcgagaac ttactatagt tttcctttgg cgaaaagcta ataatcttaa
     7141 atcttgattt tgtcctcttt tctctgagtt agattttctt aaattccact tccgacctat
     7201 taagaaatgg gcttttgcaa agaagatccg cttcactgag cccgtatctc gaagaggata
     7261 atacaacaac aaagcaaaac ggcacgtagt tttaattgta accaaggatt gcatttcggt
     7321 cttgtttcaa caaacgaaac ttcctgaaat gccaagaaaa atctggtcat ttcaccacag
     7381 tgatcattgt gtatgtgttc taaagactcc aagcgaaggt tttagaaaaa ggagcatttt
     7441 ctattctatt caagaaactc gaagaacatt ctctcttcat cctctaactt ccctataaat
     7501 atgtcctttg ctaatcagat caaatcagca ggaaaatcaa gaaccaaaag tctcccgaaa
     7561 agcaacgaaa tggctcctaa gaagaagagg aaggtgatga gccagttcga catcctgtgc
     7621 aagacccccc ccaaggtgct ggtgcggcag ttcgtggaga gattcgagag gcccagcggc
     7681 gagaagatcg ccagctgtgc cgccgagctg acctacctgt gctggatgat cacccacaac
     7741 ggcaccgcca tcaagagggc caccttcatg agctacaaca ccatcatcag caacagcctg
     7801 agcttcgaca tcgtgaacaa gagcctgcag ttcaagtaca agacccagaa ggccaccatc
     7861 ctggaggcca gcctgaagaa gctgatcccc gcctgggagt tcaccatcat cccttacaac
     7921 ggccagaagc accagagcga catcaccgac atcgtgtcca gcctgcagct gcagttcgag
     7981 agcagcgagg aggccgacaa gggcaacagc cacagcaaga agatgctgaa ggccctgctg
     8041 tccgagggcg agagcatctg ggagatcacc gagaagatcc tgaacagctt cgagtacacc
     8101 agcaggttca ccaagaccaa gaccctgtac cagttcctgt tcctggccac attcatcaac
     8161 tgcggcaggt tcagcgacat caagaacgtg gaccccaaga gcttcaagct ggtgcagaac
     8221 aagtacctgg gcgtgatcat tcagtgcctg gtgaccgaga ctaagacaag cgtgtccagg
     8281 taagtttctg cttctacctt tgatatatat ataataatta tcattaatta gtagtaatat
     8341 aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt gcttttctgt
     8401 agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca aaatttgttg
     8461 atgtgcaggc acatctactt tttcagcgcc agaggcagga tcgaccccct ggtgtacctg
     8521 gacgagttcc tgaggaacag cgagcccgtg ctgaagagag tgaacaggac cggcaacagc
     8581 agcagcaaca agcaggagta ccagctgctg aaggacaacc tggtgcgcag ctacaacaag
     8641 gccctgaaga agaacgcccc ctaccccatc ttcgctatca agaacggccc taagagccac
     8701 atcggcaggc acctgatgac cagctttctg agcatgaagg gcctgaccga gctgacaaac
     8761 gtggtgggca actggagcga caagagggcc tccgccgtgg ccaggaccac ctacacccac
     8821 cagatcaccg ccatccccga ccactacttc gccctggtgt ccaggtacta cgcctacgac
     8881 cccatcagca aggagatgat cgccctgaag gacgagacta accccatcga ggagtggcag
     8941 cacatcgagc agctgaaggg cagcgccgag ggcagcatca gataccccgc ctggaacggc
     9001 atcatcagcc aggaggtgct ggactacctg agcagctaca tcaacaggcg gatctgagct
     9061 tctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
     9121 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
     9181 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     9241 caaaatccag tactaaaatc cagatcgctc agagaggatg cacatgtgac cgagggacac
     9301 gaagtgatcc gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     9361 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     9421 gtccatttgt atgtgccagc cgcctttgcg acgctcaccg ggctggttgc cctcgccgct
     9481 gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg
     9541 agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga
     9601 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag
     9661 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac
     9721 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg
     9781 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
     9841 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
     9901 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
     9961 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
    10021 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
    10081 taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc
    10141 aaaaatggca gcgctggcca attcccgagt gcgcggaacc cctatttgtt tatttttcta
    10201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    10261 ttgaaaaagg aagagtatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga
    10321 aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt
    10381 gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta
    10441 tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa
    10501 ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg
    10561 cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta
    10621 tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag
    10681 cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga
    10741 ttgcgaaaac tgggaagagg acactccatt taaagatccg cgcgagctgt atgatttttt
    10801 aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa
    10861 catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc
    10921 ggacaagtgg tatgacattg ccttctgcgt ccggtcgctc agggaggata tcggggaaga
    10981 acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa
    11041 atattatatt ttactggatg aattgtttta gctgtcagac caagtttact catatatact
    11101 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    11161 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    11221 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    11281 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    11341 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    11401 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    11461 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    11521 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    11581 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    11641 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    11701 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    11761 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    11821 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcagatc ctagatgtgg
    11881 cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa gtgttttggc
    11941 tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt cgtgcagggc
    12001 aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac cgacttcatt
    12061 gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca ggaataaggg
    12121 cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt
    12181 gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga ggatgccgaa
    12241 accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg
    12301 atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc tccccctgcc
    12361 ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc ttgaacagga ggcggcaggt
    12421 ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc
    12481 gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt gctgaaacac
    12541 acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc gtggccggac
    12601 acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac gcgcaacaag
    12661 aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag
    12721 atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg
    12781 ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt ctacgagctt
    12841 tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga ggaatgcctg
    12901 tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca cctggaatcg
    12961 gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag
    13021 gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac gaaattcata
    13081 tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta tttcagctcg
    13141 caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg atcggattcc
    13201 acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc
    13261 ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg ctagggcctt
    13321 gtggggtcag ttccggctgg gggttcagca gcccctgctc ggatctgttg gaccggacag
    13381 tagtcatggt tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
    13441 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
    13501 aacttaataa cacattgcgg acgtttttaa tgtactgggg ttgaacactc t
//
