LOCUS       sequence-378010-       12635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral 
            Replication: WDV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_213
FEATURES             Location/Qualifiers
     source          1..12635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(379..1173)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1598..1622
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1700..1874
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1915..2940)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             complement(1915..2940)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(3007..3683)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(3050..3070)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3874..3895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3910..3915,3916..3933,3934..3940)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3948..3964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3953..3975
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3972..3988
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3972..3988
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4446..4570
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4595..4600,4601..4618,4619..4625)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4679..5359
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5679..5984
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5863..5882
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6025..6149)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7953..7977
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9278..9907
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10336..11409
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11475..11669
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11905..11927)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12013..12153
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12068..12087
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12168..12185)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12339..12635,1..292))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12419..12438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
       61 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      121 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
      181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      301 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      361 ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
      421 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      481 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      541 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      601 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      661 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      721 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      781 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      841 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      901 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      961 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
     1021 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
     1081 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
     1141 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
     1201 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
     1261 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
     1321 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
     1381 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     1441 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     1501 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     1561 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     1621 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     1681 acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga
     1741 aattttattg atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct
     1801 caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca
     1861 ttattatgga gaaactcgag cttgtcgatc gacagatccc ggtcggcatc tactctattt
     1921 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     1981 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     2041 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     2101 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg
     2161 tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     2221 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     2281 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     2341 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     2401 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     2461 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     2521 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     2581 atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     2641 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc
     2701 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     2761 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     2821 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggacacgct
     2881 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     2941 atctcattgc cccccggatc tgcgaaagct cgagagagat agatttgtag agagagactg
     3001 gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     3061 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
     3121 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     3181 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
     3241 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
     3301 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     3361 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     3421 ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     3481 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     3541 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     3601 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     3661 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     3721 tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg
     3781 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     3841 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     3901 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     3961 acaatttcac acaggaaaca gctatgacat gattacgaat tcgagtaagg tagtgaacag
     4021 aagtccggca ggtccttagc gaaaaaacgg ggtgtgccag aaaactctat cctctaccct
     4081 gcgtggaggt gtgaattctg cacactgcaa atgcaatgtg tccaatgctt tatatagggc
     4141 aggttttggc gggagaacag ggccctagtg ttcccacggt agcgtagcga atcgtgtggg
     4201 ccctgttcgg tgtgcggtcg gggggcctcc acgcgggtta taatattacc ccgcgtggtg
     4261 gcccccgacg cgcactcggc ttttcgtgag tgcgcggagg cttttggacc acatcttttc
     4321 tgatcacttt cgtggaagat gttgatttat cacacttttg acggggaaat ctgtgccatg
     4381 ccttagctta taaggaagtg cgtggtagcc catctcgaca agtttgtacc gatcaaaagc
     4441 aggtgacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata
     4501 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
     4561 agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     4621 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
     4681 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     4741 ttttgaggca tttcagtcag tcgctcaatg tacctataac cagaccgttc agctggatat
     4801 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     4861 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
     4921 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     4981 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     5041 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     5101 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     5161 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     5221 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     5281 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcaggcgg ggcgtaatct
     5341 agaggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
     5401 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
     5461 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     5521 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     5581 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     5641 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
     5701 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     5761 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtccccc
     5821 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     5881 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     5941 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
     6001 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
     6061 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     6121 tctcgttcag ctttcttgta caaagtggtc acctgcaaaa agtgtttgat cgccggcggt
     6181 acaaagtggt taaaataata ttttatttat ctcatgtcat tcgattacag aggctcggct
     6241 acgagcaaag acaaaccaaa tataacaaac aacaaccctt acacaatgac atcggaaaac
     6301 gaaatacaac accctgagat attacattta tagaaactgt acgccgtccg cgctaggaca
     6361 gtcactgcga agcagtgacg tcttcgccgg aggcgaacga gtagttgatg aacgtctcgc
     6421 cttcatacat gtagtgaaca acagtgttag agtacatgta atccgactgt tcgggagtca
     6481 tatccttgag ccaatcttcg tctggattaa ctaaaatgat gcaaggtatt ccaccccgta
     6541 tgacctttcg cttaccatat tttggattga ccgtgaagtc acgctgagcc ccgacgaagc
     6601 acttccagtt gggtgtgaac ttgaatggaa tgtcgtcgat gatattatac ttggcgttga
     6661 cgtcatatgt tgtgaaatca actagactgt tataataatt gtgtgtccct agagaccttg
     6721 cccaggaagt ctttcctgtt ctggttggcc cgcagatgta gatggactta tgcctccccg
     6781 gtgactcctg gaataatcgt ccatccactc taagtcagat tgcgcttgat ccgcaggagt
     6841 ggaagtacaa aggatatagg attcgaggct tacggagtag agatgttcat ttttccagct
     6901 ttcaatggtc tcatggcaaa tgagtgattc ggttggaaac tcaggtgtgt aagtggcaac
     6961 tgggtcagga aatagatggc gtgccgtgta ctcgaagtct ttgagacgga tagaccattc
     7021 aaacggaaaa cgattgcaaa ccatgctgag gaattcctcg cgagaggaac tagattcaat
     7081 gatctgtttc atatccgcat cacggtcttt acgacctgga gttgaaacag ccacgaatgt
     7141 tccccactca gctgtgttta catcggagtc aacctccttc gtgatgtaat cacgaacttg
     7201 gttgcagtct ttggcagctt gtatatttgg atggaatatg gagaatggag atgtatccat
     7261 acggaggttt aaggcattgg gattggtgat ggaagcacga agcttgttct gcacgagaac
     7321 gtgcagatgt ggtgatccat cttcgtggag ctctctaaca gcagcgatgt agaggggctc
     7381 atatttgttc aagagagtgc gaagtgaatc caaggcgtac tgtggctcaa gggtacattg
     7441 aggatatgtt agaaagaggt acttggaata gacacggaac ctgggtgcag atgaagaggc
     7501 catggtagtg aacagaagtc cggcaggtcc ttagcgaaaa aacggggtgt gccagaaaac
     7561 tctatcctct accctgcgtg gaggtgtgaa ttctgcacac tgcaaatgca atgtgtccaa
     7621 tgctttatat agggcaggtt ttggcgggag aacagggccc tagtgttccc acggtagcgt
     7681 agcgaatcgt gtgggccctg ttcggtgtgc ggtcgggggg cctccacgcg ggttataata
     7741 ttaccccgcg tggtggcccc cgacgcgcac tcggcttttc gtgagtgcgc ggaggctttt
     7801 ggaccacatc ttttctgatc actttcgtgg aagatgttga tttatcacac ttttgacggg
     7861 gaaatctgtg ccatgcctta gcttataagg aagtgcgtgg tagcccatct cggggccctc
     7921 gattcgacgt tcctgtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     7981 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     8041 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     8101 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     8161 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     8221 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     8281 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     8341 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     8401 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     8461 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     8521 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     8581 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     8641 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     8701 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     8761 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     8821 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     8881 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     8941 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     9001 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     9061 tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     9121 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     9181 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     9241 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     9301 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     9361 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     9421 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     9481 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     9541 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     9601 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     9661 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     9721 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     9781 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     9841 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     9901 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     9961 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
    10021 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
    10081 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
    10141 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
    10201 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
    10261 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa
    10321 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
    10381 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
    10441 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
    10501 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
    10561 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
    10621 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
    10681 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
    10741 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
    10801 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
    10861 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
    10921 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
    10981 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
    11041 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
    11101 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
    11161 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
    11221 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
    11281 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
    11341 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
    11401 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
    11461 aaaggcctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
    11521 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
    11581 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
    11641 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc ccaagagctg
    11701 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
    11761 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
    11821 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
    11881 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaaa cggtcacagc
    11941 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    12001 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
    12061 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    12121 cacagatgcg taaggagaaa ataccgcatc aggccctctt ccgcttcctc gctcactgac
    12181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    12241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    12301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    12361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    12421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    12481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    12541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    12601 ccccccgttc agcccgaccg ctgcgcctta tccgg
//
