LOCUS       sequence-378046-       18190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for dCasEV2.1 activated expression of ATF1-2, HarFAR and Atr 
            delta11..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18190)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 18190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB4361
FEATURES             Location/Qualifiers
     source          1..18190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             183..1256
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1322..1516
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1752..1774)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1860..2000
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1915..1934
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2015..2032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2186..2774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2266..2285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2862..3677)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2862..3677)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3585..3604
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    4110..4134
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(4179..4431)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4471..5265)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4582..4601)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5192..5211
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5299..5478)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    15990..16014
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             17315..17944
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
       61 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
      121 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
      181 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
      241 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
      301 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
      361 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
      421 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
      481 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
      541 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
      601 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
      661 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
      721 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
      781 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
      841 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
      901 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
      961 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     1021 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     1081 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     1141 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     1201 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     1261 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggactctttc
     1321 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     1381 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     1441 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     1501 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     1561 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     1621 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     1681 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     1741 gacggtgaaa acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg
     1801 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     1861 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     1921 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1981 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2041 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2161 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2221 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2281 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2341 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2401 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2521 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2581 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2641 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2701 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2761 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2821 aaaactcacg ttaagggatt ttggtcatgc attctaggtg attagaaaaa ctcatcgagc
     2881 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2941 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     3001 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     3061 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     3121 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     3181 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat
     3241 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     3301 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     3361 gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     3421 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     3481 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     3541 ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     3601 tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc
     3661 cgttgaatat ggctcataac agaacttatt atttccttcc tcttttctac agtatttaaa
     3721 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     3781 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     3841 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
     3901 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
     3961 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
     4021 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     4081 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataacg
     4141 aattcgtctc aggagagcga tcagcttgca tgccggtcga tctagtaaca tagatgacac
     4201 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat
     4261 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat
     4321 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tggcaagacc
     4381 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttgact
     4441 ctagctagag tccgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     4501 ggctatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     4561 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     4621 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     4681 attcggcaag caggcgtcgc cgtgggtcac gacgagatcc tcgccgtcgg gcatccgcgc
     4741 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     4801 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcggt gtttcgcttg
     4861 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     4921 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     4981 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     5041 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttggagtt cattcagggc
     5101 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     5161 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     5221 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcctc gatcgagttg
     5281 agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca
     5341 tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat
     5401 aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg ctcagtggct
     5461 ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc
     5521 gggggtcata ccgtgactcc cttaattctc atgtatctcc tgacggagtg tttcatgtcc
     5581 aagcgaaaag ccgctctacg gaatggatct acgttactgc ctgcataagg agatcggtgt
     5641 agccaaggac gaaagcgacc ctaggttcta accgtcgact ttggcggaaa ggtttcactc
     5701 aggaagcaga cactgattga cacggtttag cagaacgttt gaggactagg tcaaattgag
     5761 tggtttaata tcggcatgtc tggctttaaa attcagtata gtgcgctgat cggagatgaa
     5821 ttaaaaacac gagttcccaa aaccaggcgg gctcgccacg tcggctaatc ctggtacatt
     5881 atgtgaacaa tgttctgaag aaaatttgtg aaagaaggac gggtcatcgc ctactattag
     5941 caacaacggt cggccacacc ttccattgtc gtggccacgc tcggattaca cggcagaggt
     6001 gcttgtgttc cgacaggcta gcatattatc ctaaggcgtt accccaatcg tttaccgtcg
     6061 gatttgctat agcccctgaa cgctacatgt acgaaaccat gttatgtatg cactaggtca
     6121 acaataggac atagccttgt agttaacacg tagcccggtc gtataagtac agtagaccct
     6181 tcgccggcat cctatttatt aagttatttc tacagcaaaa cgatcatatg cagatccgca
     6241 gtgggcggta gacacacgtc cacccggctg ctctgtaaca gggactaaag aggtgatgat
     6301 tatcgtgtgt gccccgttat ggtcgtgttc gatcagagcg cccttgcgag cagtcgtatg
     6361 ctttctcgaa ttccgagcgg ttaagcgtga cagtcccagc gaacccacaa aacgtgatcg
     6421 cagtccatgc gatcatacgc aagaaggaag gtccccatac accgacgcac cagtttacac
     6481 gccgtatgca taaacgagct gcacaaacga gagtgtttga actggacctc tagttcctct
     6541 acaaagaaca ccttgacctg ttgcgaagtt gccttgccta gatgcaatgt cggacgtatc
     6601 acttttgcct caacgactgc tgctttcgct gtaacccaag acagacaaca gtaaccgcct
     6661 tttgaaggca agtcctccgc ctgtgactaa ctgtgccaaa tcgtcttcca aaccccttat
     6721 ccaatttaac tcaccaaatt attgcgatac agaccctaat ttcacatcat atgacactaa
     6781 ttgcctctgc caaaattctg tcctcaatga ctccctcttc tctcaccaac cagtcagttg
     6841 ggttcacccg ggtcggacct gagtcgacca agccttcttc tctcaccaac cagtcgcgac
     6901 cgcagaactt acgctcgagg gtccgtcttc tctcaccaac cagtctcccc ggttatctct
     6961 cccgtcctaa tacctctacg tgcaagcagt ataaaaacaa aatacataaa cataactaaa
     7021 atctcatatc gttgtaattg tcatatacta gtggtaatta agtccagtta cacttaaggt
     7081 taaatttgct gactcttttt tacctttcat ttctgaaaat gaatgaaatc gatgaaaaaa
     7141 atcaagctcc cgtacaacag gagtgtctca aggaaatgat acagaacggc cacgcaagga
     7201 ggatgggcag tgtagaggac ctctacgtag ccttaaacag acaaaacctg tatagaaact
     7261 tttgtacgta tggggaactt tccgactact gcacccgaga tcaactgaca ttagccctga
     7321 gggagatttg tctcaaaaat ccaacccttc tgcatatagt cttgccaacc cgttggccaa
     7381 accatgagaa ctattatcgt tcttccgaat attattcaag accccatcct gtccatgatt
     7441 atataagcgt actccaagag ttgaagcttt ctggggtagt gcttaatgaa caaccggaat
     7501 actctgcagt gatgaagcaa atccttgaag aatttaagaa ttcaaaaggt agctatacgg
     7561 caaaaatctt caagttgaca actactttga cgatacccta cttcgggcct accggtcctt
     7621 catggagatt gatctgtctc cccgaggaac atacagagaa gtggaggaag ttcatttttg
     7681 ttagcaacca ctgtatgtca gatggcaggt ccagtataca ttttttccat gatcttagag
     7741 atgagcttaa caatataaag acccctccaa agaagttaga ctatatcttt aagtatgaag
     7801 aggattacca gttattaagg aaactccctg agcctataga gaaagttata gactttaggc
     7861 caccgtattt attcatcccg aagagccttc ttagcggatt catctataac cacttacgat
     7921 ttagttccaa aggagtgtgt atgcgaatgg atgacgttga gaaaacggat gacgtcgtaa
     7981 cagaaataat aaatattagc cctacagaat ttcaagctat caaggccaat attaagagta
     8041 acatccaagg gaaatgtacg atcacgccct ttttgcatgt gtgttggttt gtatcccttc
     8101 acaagtgggg aaaatttttc aagcccctta acttcgaatg gctgactgac atttttatac
     8161 cagctgattg caggtcccaa ctcccagacg atgatgaaat gcgacagatg tatagatatg
     8221 gcgctaatgt agggtttata gacttcactc cctggataag cgagtttgat atgaacgaca
     8281 acaaagaaaa cttctggccc ttaattgaac actaccacga ggttatttct gaagccttga
     8341 ggaataagaa gcatcttcat ggccttggct ttaacattca gggattcgtt caaaagtatg
     8401 tcaatataga taaggttatg tgcgaccgtg ctattggaaa aagacgtggc ggtacgctct
     8461 tgagcaatgt gggtttgttt aatcaattgg aagagccgga cgccaaatat tctatatgcg
     8521 acttagcctt tggtcaattt cagggaagct ggcaccaagc cttcagtctt ggagtatgta
     8581 gcactaacgt caaaggaatg aacatagtgg ttgcatcaac taaaaacgtg gtaggatctc
     8641 aggagagctt agaggaatta tgttccattt acaaagctct gctgttaggg ccttaggctt
     8701 acgaaaatat agttgaaaca gagcagagat catggtgtta gccttctatg gctgaagaaa
     8761 aatagttgaa aaaatgtgtg tgtctagtgt tttagtgtgt ctgtctgttg aaaacaggga
     8821 aaaaaatgtg ttcttttttc cctgtaataa gaaatatagt aatggagtct ttatgtgtaa
     8881 gtaaccagta aaatggtttg tgtaaacatg aatatctctg catctgttaa ttttaacata
     8941 taagtttgga tatgtgttgt ggtttcaact aattcaagta cttccttgat aatgttttcc
     9001 tgtttctagg ctgcaaaact atagtaaatc ttttaggttt tggtaaaacc taattccatt
     9061 gggtgtggtt ggagagcata tagtttttgc gacgctctcc gtcaggagcc ctgatttttt
     9121 cactactaat agtactcacg gcgcaatacc agcacagcct agtctcgcca gaatgctggt
     9181 cagcatacga aagagcttaa ggcaggccaa ttccactgtc agggtcactt gggtgtttag
     9241 cactaccgac aggtacgcta gtatgcgttc ttcctaccag aggtctgtgg ccgcgtggtc
     9301 aaaagtgcgg ctttcgtatt tgctgctcgt gtttactctc acaaacttga cctgcacgcc
     9361 aaagagatgc ttcttgtgga actcgacaac gcaacaacgc gacggatcta cgtcacagcg
     9421 tgcatagtga aaacggagtt gctgacgacg aaagcgacat tgggatctgt cagttgtcat
     9481 tcgcgaaaaa catccgtccc cgaggcggac actgattgac gcggttttgt agaaggttag
     9541 gggaataggt tagattgagt ggcttaagaa tgtaaaatct gggattatag tgtagtaatc
     9601 tctgattaac ggtgacggtt ttaagacagg tgttcgcaaa atcaagcggg gtcatttcaa
     9661 cagatattgc tgatggttta ggcgtacaat gccctgaaga ataattaaga aaaaagcacc
     9721 cctcgtcgcc tagaattacc taccgcggtc caccatacct tcgattatcg cgcccactct
     9781 cccattagtc ggcagaggtg gttgtgttgc gatagcccag tatgatattc taaggcgtta
     9841 cgctgatgaa tattctacag agttgccata ggcgttgaac gctacacgga cgatacgaat
     9901 ttacgtatag agcgggtcat cgaaaggtta tactctcgta gttaacatct agcccggccc
     9961 tatcagtaca gcagtgcctt gaatgacata ctcatcatta aattttctct acagccaaac
    10021 gaccaagtgc atttccaggg agcgcgatgg agattcattc tctcgccagc actgtaatag
    10081 gcactaaaag agtgatgata atcatgagtg ccgcgctaag gtggtgtcgg aacaaagcgg
    10141 tcttacggtc agtcgtattc cttctcgagt tccgtccagt tgagcgtgtc actcccagtg
    10201 tacctgcaag ccgagatggc tgtgcttgga gtcaatcgca tgtagcatgg tcaccagaca
    10261 ccggggcacc agttttcacg cctaaagcat aaacgacgag cagtcatgaa agtcttagta
    10321 ctggacgtgc cgtttcactg cgaatatgac tccctcttct ctcaccaacc agtcacgacc
    10381 gcttcacgct aaggtgctgg ccacgtgcta accttcttct ctcaccaacc agtctggcgt
    10441 tgatgtggat cgactctata gccatcttct ctcaccaacc agtccgaggc cgttcgtttc
    10501 ccgtcctaat acctctacgt gcaagcagta taaaaacaaa atacataaac ataactaaaa
    10561 tctcatatcg ttgtaattgt catatactag tggtaattaa gtccagttac acttaaggtt
    10621 aaatttgctg actctttttt acctttcatt tctgaaaatg gttcctaata agggttcttc
    10681 tgatgtcttg tccgaacatt ctgagccaca gttcactaag ctgatcgccc ctcaagccgg
    10741 accaaggaag tataagatcg tgtaccgaaa cttactgact ttcggatatt ggcacctttc
    10801 tgctgtttat ggcttgtacc tttgtttcac ttgtgctaaa tgggcaacga ttttgtttgc
    10861 cttttttctt tacgtgatag ctgaaattgg gattactggt ggagctcaca gactttgggc
    10921 acatagaact tacaaggcta aactgcccct agaaattctt cttctcatta tgaactccat
    10981 agcttttcaa gatactgcat ttacttgggc cagggaccat agacttcacc acaaatactc
    11041 agatactgat gcagatcctc acaacgcaac ccggggtttt ttttattccc acgttgggtg
    11101 gttgttggtc aaaaagcatc cagaggttaa ggctagagga aagtacttgt ctctcgatga
    11161 cttaaagaat aacccgctat tgaagttcca aaaaaaatac gccatactcg taattggaac
    11221 cttatgtttt ttgatgccta cgttcgtccc ggtatacttt tggggtgaag gcatctctac
    11281 tgcatggaac atcaatcttt tacgatatgt catgaactta aatatgactt tcctagtcaa
    11341 ctctgctgcc catatatttg ggaacaagcc gtatgataaa tcaatagctt ctgtgcaaaa
    11401 tatatcagtt tccctggcta catttgggga gggtttccat aattatcatc acacatatcc
    11461 ctgggattat cgtgcagcag aacttggaaa caatagattg aatatgacaa ccgcctttat
    11521 tgatttcttc gcctggatag gttgggcata tgatctaaag tctgtgccac aagaggctat
    11581 tgcaaaacgg tgcgcaaaaa ctggagatgg tactgatatg tggggtagga aacgttgagc
    11641 ttaaacccaa ccatacataa taaaaaggcg agctacctca ccaacatgtt cttggttctt
    11701 gatggctgct tgaggttttt caacacaaca tgagtaaaat ggtcaataaa tatggatatt
    11761 ctcatcttgc ttgattcggg caggtaggat atcagagaat tctaggtata ttttgtaggg
    11821 aaaaaaaact agaatctctt caggatctgg atcctaatac aaggacatat taatattcca
    11881 ttttataaac tttgcacaag caagatctaa ttttatggaa gaactgaagg ctctcgcgta
    11941 tcatttgcca aaataattac catcttttgt aatcatcttg gaaactagag ataatattga
    12001 taatatccat agtaataata ataataagaa gaacaaatat catcataaca caataatata
    12061 gccttttcat gtaaaatttc atttcaatga atagccatgt tagcccattt tctttcagga
    12121 aagtttatgc ttgcttacat gaagaaacaa aaaaaattat gagaaacaga atttactcac
    12181 tactgtgcaa attatatgtt caactgagca aacaagtctt caactacctc tcctcagagt
    12241 tgaatctgtt ccatgcttcc tatagacaca aaagaacgat aaccactaag ataattcaaa
    12301 gaagataata aaagagaatg gaccatggga agaagagggg gaggtgaagc tggcgctgtc
    12361 aggagctaac tgagatacta ccatagatga ctacccatcc ctctaggcct tagataaccg
    12421 gatacagtga ctttgatagg tttgtggggt acagctatga cttgcatagc tgcgtatgga
    12481 ggaaggaact tttgcgtgtt agtatgttga cccgtgtatt acgcatgcgg gtagatcatg
    12541 tgggtagaga catccaggtc aagttctcga ccttctcgtg ggaggtgatc cagttcacta
    12601 taggaccatt ccattcgagc atggcactaa gtacgccgtc cccattctgg taatcatcat
    12661 ccctatcagg gtttggagtg agtggtgacg gttattcccc aggaatggac ttcttattca
    12721 cagtcggtca cattgggcta ctccttgggt cttccgcttg gcccggtctg ttgggccgcc
    12781 attgcgtgag tttcggccct gtgctgcgct gtgtcggcga ttctcattgg ggcctcacat
    12841 ctggaaaccc cgacctattt tgacggcacc attggcggaa gttgctgggc ttgtgcaccg
    12901 tgaagtcctc cgcgggcctc ccccctacaa aagacgataa gctccggcaa gcagcattga
    12961 tcaacgcaag gatcggcgat attaacagag atacggctga ttactcttgt tggtgtggta
    13021 tcgctaaact gcgccgcgga gccttatggc aaagtcgtcc gcgaaccatt ccggtagcgc
    13081 ttaaggtcca tagcacattc atcgcatccc ggcgtgcgtt caatttgacg accccttggc
    13141 gcaaaggtgc tggccacgtg ctaaattaaa gcggctgcac tgctgtaagg tccgttacgg
    13201 agagggcggc ctggggagag cactagccca tcgacctgta caggaacact ctatattgtt
    13261 ctcggacgga cagattacta gagtgccgct ttcagccccc ctgtcgtcgc cgacgtctgt
    13321 aatatggcgt tgttgtgatt ccactctatt gaggcattaa ctgatgcgta aggagatctg
    13381 gaatgaactg gcctatgtca ctgaaactgt gcaaacaccc aatgtcgtta gtgtaggttc
    13441 tgaccgatac ctgcttcgtt gagaactcac aattttacaa ctggggacat aatccctacg
    13501 cccatcatct actcgcgtcc ctgtggctcc agttcatgta atgggagagt atcctccaca
    13561 agatctagtg ctatggtggt atagtaagct cgtactgtgg tagatgactc cctcttctct
    13621 caccaaccag tcaacgcccc gtaccgatgc tgaacaagtc gatgcaggcc cttcttctct
    13681 caccaaccag tccaatcccg aggcctgacc cgacatatcc catcttctct caccaaccag
    13741 tcgtagctaa ctatgttccc gtcctaatac ctctacgtgc aagcagtata aaaacaaaat
    13801 acataaacat aactaaaatc tcatatcgtt gtaattgtca tatactagtg gtaattaagt
    13861 ccagttacac ttaaggttaa atttgctgac tcttttttac ctttcatttc tgaaaatggt
    13921 tgtgttgact agcaaggaga caaagcctag cgtcgctgaa ttttacgctg gaaaaagtgt
    13981 ttttattact gggggtactg ggtttctcgg taaggttttc attgagaaac tcttgtattc
    14041 ttgtccagat atagaaaaca tttacatgtt gatcagggaa aaaaaaggtt tatcagttag
    14101 tgagaggatc aagcagtttc ttgacgaccc tctgtttacc aggttgaagg ataagaggcc
    14161 tgctgatctt gagaaaatcg tccttattcc tggcgacatt actgctcccg acttgggtat
    14221 taacagtgag aacgaaaaaa tgcttataga aaaggtgagt gttatcatac actcagcagc
    14281 taccgtcaag ttcaatgagc cattgcctac cgcttggaag atcaatgtcg agggtacacg
    14341 gatgatgtta gcacttagca gacggatgaa gagaatagag gttttcatcc atatctctac
    14401 cgcttacaca aatacaaaca gggaggtggt tgatgaaatt ttgtaccctg cacctgctga
    14461 cattgaccaa gttcaccagt acgtgaaaga tggtatctct gaagaagata ctgagaagat
    14521 cctcaatgga aggcccaata catatacttt caccaaggct ctgaccgagc atttagttgc
    14581 tgagaaccaa gcttatgtgc caaccattat cgttaggccg agtgttgttg ctgctataaa
    14641 ggacgagcca cttaaaggat ggctgggcaa ttggtttggc gctaccggat tgacagtttt
    14701 taccgcaaag ggactaaaca gggtcattta tggtcattcc tcatacatag ttgatcttat
    14761 tcctgtagat tacgttgcta atttagttat cgccgcagga gccaagtctt caaaatctac
    14821 agaattgaaa gtgtataatt gctgctcttc aagctgcaac ccagtgacca ttgggacgtt
    14881 aatgagtatg ttcgctgacg acgctataaa gcagaaaagt tatgcaatgc cgttaccggg
    14941 ttggtacata tttaccaagt ataagtggct tgtgctactg ttgactttct tatttcaggt
    15001 cattcctgct tatgttacag atctctctag gcatctgatt ggtaaatccc cacgttatat
    15061 caagctccaa tccctggtta atcagactag atcatctata gatttcttta ccaaccacag
    15121 ctgggttatg aaggccgacc gggtgaggga gttgtacgca tcactgtctc ccgccgataa
    15181 atacctattc ccgtgtgatc ctaccgatat aaattggact cattatatac aggattattg
    15241 ttggggtgtg aggcacttct tggaaaagaa atatcggtga gcttctttgt ggttattatt
    15301 tagcttctgt acactaaatt tatgatgcaa gaagcgttgt acacaacata tagaagaaga
    15361 gtgcgaggtg aagcaagtag gagaaatgtt aggaaagctc ctatacaaaa ggatggcatg
    15421 ttgaagatta gcatcttttt aatcccaagt ttaaatataa agcatatttt atgtaccact
    15481 ttctttatct ggggtttgta atccctttat atctttatgc aatctttacg ttagttaata
    15541 tctatctatc gatattctag tatcttatac tatagatcca actgaaccaa gaaattatga
    15601 accgtgtctt ccagaaattc taataatgat gggagcaata taaatataag gatgtctttg
    15661 acaataaaag ggcggtggaa gagttatagt gaaagcttct ggccatcaaa tgggctcttt
    15721 gtcacatgat tgaactactt caccacctac cgttggtatc catagtaaat tgagattaaa
    15781 aaaagttaaa acaaactatt cattccatgt cattttactt ttcaacttgc tatcttctaa
    15841 ttataagtgg gttgtggaga gagcactgtt gggtcagcaa agcaaagtag attatactct
    15901 ataatattat gatgattagt caactgctgc tcacttacaa cgatggaaat ggggatgagg
    15961 gtaactttac gctgtcatga gacgaattct gacaggatat attggcgggt aaacctaaga
    16021 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
    16081 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
    16141 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa
    16201 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
    16261 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
    16321 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
    16381 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
    16441 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
    16501 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
    16561 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
    16621 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
    16681 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
    16741 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
    16801 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
    16861 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
    16921 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
    16981 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
    17041 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
    17101 agccgcccgc gcacctctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
    17161 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
    17221 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
    17281 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
    17341 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
    17401 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
    17461 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
    17521 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
    17581 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
    17641 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
    17701 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
    17761 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
    17821 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
    17881 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
    17941 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
    18001 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
    18061 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
    18121 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
    18181 atgagcaaat
//
