LOCUS       sequence-378050-       18190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for dCasEV2.1 activated expression ATF1, Atr delta11 and 
            HarFAR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18190)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 18190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB4360
FEATURES             Location/Qualifiers
     source          1..18190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             171..1244
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1310..1504
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1740..1762)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1848..1988
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1903..1922
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2003..2020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2174..2762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2254..2273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2850..3665)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2850..3665)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3573..3592
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    4098..4122
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(4167..4419)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4459..5253)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4570..4589)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5180..5199
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5287..5466)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    15978..16002
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             17303..17932
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
       61 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
      121 gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
      181 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
      241 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
      301 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
      361 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
      421 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
      481 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
      541 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
      601 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
      661 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
      721 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
      781 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
      841 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
      901 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
      961 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     1021 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     1081 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     1141 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     1201 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     1261 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg actctttcct gtggatagca
     1321 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     1381 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     1441 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     1501 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     1561 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     1621 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     1681 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     1741 ctctgacaca tgcagctccc ggtgacggtc acagcttgtc tgtaagcgga tgccgggagc
     1801 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     1861 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     1921 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     1981 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2041 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2101 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2161 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2221 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2281 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2341 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2401 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2461 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2521 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2581 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2641 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2701 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2761 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2821 aagggatttt ggtcatgcat tctaggtgat tagaaaaact catcgagcat caaatgaaac
     2881 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     2941 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     3001 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     3061 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     3121 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     3181 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gtcgaaatac gcgatcgctg
     3241 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     3301 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct
     3361 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     3421 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     3481 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     3541 cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     3601 tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg ttgaatatgg
     3661 ctcataacag aacttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
     3721 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
     3781 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
     3841 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
     3901 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
     3961 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
     4021 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
     4081 agctgttggc tggctggtgg caggatatat tgtggtgtaa acataacgaa ttcgtctcag
     4141 gagagcgatc agcttgcatg ccggtcgatc tagtaacata gatgacaccg cgcgcgataa
     4201 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     4261 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     4321 atgcttaacg taattcaaca gaaattatat gataatcatg gcaagaccgg caacaggatt
     4381 caatcttaag aaactttatt gccaaatgtt tgaacgatct gcttgactct agctagagtc
     4441 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg ctatgcgctg
     4501 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     4561 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     4621 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     4681 ggcgtcgccg tgggtcacga cgagatcctc gccgtcgggc atccgcgcct tgagcctggc
     4741 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     4801 accggcttcc atccgagtac gtgctcgctc gatgcggtgt ttcgcttggt ggtcgaatgg
     4861 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     4921 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     4981 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     5041 ggccagccac gatagccgcg ctgcctcgtc ttggagttca ttcagggcac cggacaggtc
     5101 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     5161 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     5221 agaacctgcg tgcaatccat cttgttcaat catgcctcga tcgagttgag agtgaatatg
     5281 agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga
     5341 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     5401 cgtatgtgct tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg
     5461 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcatacc
     5521 gtgactccct taattctcat gtatctcctg acggagtgtt tcatgtccaa gcgaaaagcc
     5581 gctctacgga atggatctac gttactgcct gcataaggag atcggtgtag ccaaggacga
     5641 aagcgaccct aggttctaac cgtcgacttt ggcggaaagg tttcactcag gaagcagaca
     5701 ctgattgaca cggtttagca gaacgtttga ggactaggtc aaattgagtg gtttaatatc
     5761 ggcatgtctg gctttaaaat tcagtatagt gcgctgatcg gagatgaatt aaaaacacga
     5821 gttcccaaaa ccaggcgggc tcgccacgtc ggctaatcct ggtacattat gtgaacaatg
     5881 ttctgaagaa aatttgtgaa agaaggacgg gtcatcgcct actattagca acaacggtcg
     5941 gccacacctt ccattgtcgt ggccacgctc ggattacacg gcagaggtgc ttgtgttccg
     6001 acaggctagc atattatcct aaggcgttac cccaatcgtt taccgtcgga tttgctatag
     6061 cccctgaacg ctacatgtac gaaaccatgt tatgtatgca ctaggtcaac aataggacat
     6121 agccttgtag ttaacacgta gcccggtcgt ataagtacag tagacccttc gccggcatcc
     6181 tatttattaa gttatttcta cagcaaaacg atcatatgca gatccgcagt gggcggtaga
     6241 cacacgtcca cccggctgct ctgtaacagg gactaaagag gtgatgatta tcgtgtgtgc
     6301 cccgttatgg tcgtgttcga tcagagcgcc cttgcgagca gtcgtatgct ttctcgaatt
     6361 ccgagcggtt aagcgtgaca gtcccagcga acccacaaaa cgtgatcgca gtccatgcga
     6421 tcatacgcaa gaaggaaggt ccccatacac cgacgcacca gtttacacgc cgtatgcata
     6481 aacgagctgc acaaacgaga gtgtttgaac tggacctcta gttcctctac aaagaacacc
     6541 ttgacctgtt gcgaagttgc cttgcctaga tgcaatgtcg gacgtatcac ttttgcctca
     6601 acgactgctg ctttcgctgt aacccaagac agacaacagt aaccgccttt tgaaggcaag
     6661 tcctccgcct gtgactaact gtgccaaatc gtcttccaaa ccccttatcc aatttaactc
     6721 accaaattat tgcgatacag accctaattt cacatcatat gacactaatt gcctctgcca
     6781 aaattctgtc ctcaatgact ccctcttctc tcaccaacca gtcagttggg ttcacccggg
     6841 tcggacctga gtcgaccaag ccttcttctc tcaccaacca gtcgcgaccg cagaacttac
     6901 gctcgagggt ccgtcttctc tcaccaacca gtctccccgg ttatctctcc cgtcctaata
     6961 cctctacgtg caagcagtat aaaaacaaaa tacataaaca taactaaaat ctcatatcgt
     7021 tgtaattgtc atatactagt ggtaattaag tccagttaca cttaaggtta aatttgctga
     7081 ctctttttta cctttcattt ctgaaaatga atgaaattga cgaaaagaac caagcaccgg
     7141 tccagcaaga atgcctcaaa gaaatgatcc aaaacggtca tgcccgacga atggggagtg
     7201 tcgaggacct ttatgtagct ctcaatagac aaaacctgta ccgaaacttt tgcacatatg
     7261 gagaactttc agactattgt acgcgagatc aactcaccct ggctctccgt gaaatttgtt
     7321 tgaagaatcc gaccctgctt cacatcgtgt tacctacgcg ttggccaaac cacgaaaact
     7381 actatcgttc ctccgaatat tactcaagac cgcatcccgt tcacgattac atctccgttt
     7441 tgcaagaatt aaaattaagc ggggtcgtac ttaatgaaca acctgagtat tcagctgtta
     7501 tgaagcaaat tttagaagaa ttcaaaaata gtaagggttc ttacacagct aagatattta
     7561 aacttactac aactctcacg attccttact tcggccctac aggaccgtca tggcgattaa
     7621 tttgtcttcc cgaagagcat acggagaaat ggaagaaatt catattcgtt agcaatcact
     7681 gcatgagtga tggacgatct agtatacact tttttcatga tcttagagat gagcttaata
     7741 acataaagac tcctccaaaa aagctcgact acatcttcaa gtacgaggag gattatcaac
     7801 tcttaagaaa gctccccgag ccaatagaaa aggtgatcga ctttcgtccc ccatacctgt
     7861 ttataccgaa gagcctcctt tctggattta tctataacca cctgagattc tcatcaaagg
     7921 gagtctgtat gaggatggac gatgttgaaa agacagatga tgttgtcacg gaaattataa
     7981 acataagccc aactgaattc caggccataa aagcaaatat caaatcaaac atccagggaa
     8041 agtgcacgat aacacctttt ttacacgttt gttggtttgt gagcttacac aaatggggta
     8101 aatttttcaa gcccctcaat ttcgagtggc ttacagatat attcatacct gccgattgca
     8161 gatcacaact ccccgatgat gacgagatgc gtcagatgta tcgatatggt gccaatgtcg
     8221 ggttcataga cttcactcca tggatcagcg agttcgacat gaatgataat aaggagaatt
     8281 tctggccgtt aattgagcat taccacgagg taattagtga agccttaagg aataaaaagc
     8341 acctccatgg cttagggttc aacatccaag gtttcgttca gaagtatgtc aatatcgata
     8401 aagtcatgtg cgatagggct atcggcaaaa gacgaggtgg tacgctgtta tctaatgtgg
     8461 ggttatttaa tcagctggag gagccagacg ccaaatacag tatctgtgat ttggcatttg
     8521 gccaatttca gggatcatgg catcaagctt tctccttggg agtgtgttct actaatgtta
     8581 agggcatgaa catagtggtt gcctccacga aaaatgtcgt aggcagtcaa gagtctttag
     8641 aagagttgtg ttccatttat aaggctcttc tgctgggacc ataggcttac gaaaatatag
     8701 ttgaaacaga gcagagatca tggtgttagc cttctatggc tgaagaaaaa tagttgaaaa
     8761 aatgtgtgtg tctagtgttt tagtgtgtct gtctgttgaa aacagggaaa aaaatgtgtt
     8821 cttttttccc tgtaataaga aatatagtaa tggagtcttt atgtgtaagt aaccagtaaa
     8881 atggtttgtg taaacatgaa tatctctgca tctgttaatt ttaacatata agtttggata
     8941 tgtgttgtgg tttcaactaa ttcaagtact tccttgataa tgttttcctg tttctaggct
     9001 gcaaaactat agtaaatctt ttaggttttg gtaaaaccta attccattgg gtgtggttgg
     9061 agagcatata gtttttgcga cgctctccgt caggagccct gattttttca ctactaatag
     9121 tactcacggc gcaataccag cacagcctag tctcgccaga atgctggtca gcatacgaaa
     9181 gagcttaagg caggccaatt ccactgtcag ggtcacttgg gtgtttagca ctaccgacag
     9241 gtacgctagt atgcgttctt cctaccagag gtctgtggcc gcgtggtcaa aagtgcggct
     9301 ttcgtatttg ctgctcgtgt ttactctcac aaacttgacc tgcacgccaa agagatgctt
     9361 cttgtggaac tcgacaacgc aacaacgcga cggatctacg tcacagcgtg catagtgaaa
     9421 acggagttgc tgacgacgaa agcgacattg ggatctgtca gttgtcattc gcgaaaaaca
     9481 tccgtccccg aggcggacac tgattgacgc ggttttgtag aaggttaggg gaataggtta
     9541 gattgagtgg cttaagaatg taaaatctgg gattatagtg tagtaatctc tgattaacgg
     9601 tgacggtttt aagacaggtg ttcgcaaaat caagcggggt catttcaaca gatattgctg
     9661 atggtttagg cgtacaatgc cctgaagaat aattaagaaa aaagcacccc tcgtcgccta
     9721 gaattaccta ccgcggtcca ccataccttc gattatcgcg cccactctcc cattagtcgg
     9781 cagaggtggt tgtgttgcga tagcccagta tgatattcta aggcgttacg ctgatgaata
     9841 ttctacagag ttgccatagg cgttgaacgc tacacggacg atacgaattt acgtatagag
     9901 cgggtcatcg aaaggttata ctctcgtagt taacatctag cccggcccta tcagtacagc
     9961 agtgccttga atgacatact catcattaaa ttttctctac agccaaacga ccaagtgcat
    10021 ttccagggag cgcgatggag attcattctc tcgccagcac tgtaataggc actaaaagag
    10081 tgatgataat catgagtgcc gcgctaaggt ggtgtcggaa caaagcggtc ttacggtcag
    10141 tcgtattcct tctcgagttc cgtccagttg agcgtgtcac tcccagtgta cctgcaagcc
    10201 gagatggctg tgcttggagt caatcgcatg tagcatggtc accagacacc ggggcaccag
    10261 ttttcacgcc taaagcataa acgacgagca gtcatgaaag tcttagtact ggacgtgccg
    10321 tttcactgcg aatatgactc cctcttctct caccaaccag tcacgaccgc ttcacgctaa
    10381 ggtgctggcc acgtgctaac cttcttctct caccaaccag tctggcgttg atgtggatcg
    10441 actctatagc catcttctct caccaaccag tccgaggccg ttcgtttccc gtcctaatac
    10501 ctctacgtgc aagcagtata aaaacaaaat acataaacat aactaaaatc tcatatcgtt
    10561 gtaattgtca tatactagtg gtaattaagt ccagttacac ttaaggttaa atttgctgac
    10621 tcttttttac ctttcatttc tgaaaatggt tcctaataag ggttcttctg atgtcttgtc
    10681 cgaacattct gagccacagt tcactaagct gatcgcccct caagccggac caaggaagta
    10741 taagatcgtg taccgaaact tactgacttt cggatattgg cacctttctg ctgtttatgg
    10801 cttgtacctt tgtttcactt gtgctaaatg ggcaacgatt ttgtttgcct tttttcttta
    10861 cgtgatagct gaaattggga ttactggtgg agctcacaga ctttgggcac atagaactta
    10921 caaggctaaa ctgcccctag aaattcttct tctcattatg aactccatag cttttcaaga
    10981 tactgcattt acttgggcca gggaccatag acttcaccac aaatactcag atactgatgc
    11041 agatcctcac aacgcaaccc ggggtttttt ttattcccac gttgggtggt tgttggtcaa
    11101 aaagcatcca gaggttaagg ctagaggaaa gtacttgtct ctcgatgact taaagaataa
    11161 cccgctattg aagttccaaa aaaaatacgc catactcgta attggaacct tatgtttttt
    11221 gatgcctacg ttcgtcccgg tatacttttg gggtgaaggc atctctactg catggaacat
    11281 caatctttta cgatatgtca tgaacttaaa tatgactttc ctagtcaact ctgctgccca
    11341 tatatttggg aacaagccgt atgataaatc aatagcttct gtgcaaaata tatcagtttc
    11401 cctggctaca tttggggagg gtttccataa ttatcatcac acatatccct gggattatcg
    11461 tgcagcagaa cttggaaaca atagattgaa tatgacaacc gcctttattg atttcttcgc
    11521 ctggataggt tgggcatatg atctaaagtc tgtgccacaa gaggctattg caaaacggtg
    11581 cgcaaaaact ggagatggta ctgatatgtg gggtaggaaa cgttgagctt aaacccaacc
    11641 atacataata aaaaggcgag ctacctcacc aacatgttct tggttcttga tggctgcttg
    11701 aggtttttca acacaacatg agtaaaatgg tcaataaata tggatattct catcttgctt
    11761 gattcgggca ggtaggatat cagagaattc taggtatatt ttgtagggaa aaaaaactag
    11821 aatctcttca ggatctggat cctaatacaa ggacatatta atattccatt ttataaactt
    11881 tgcacaagca agatctaatt ttatggaaga actgaaggct ctcgcgtatc atttgccaaa
    11941 ataattacca tcttttgtaa tcatcttgga aactagagat aatattgata atatccatag
    12001 taataataat aataagaaga acaaatatca tcataacaca ataatatagc cttttcatgt
    12061 aaaatttcat ttcaatgaat agccatgtta gcccattttc tttcaggaaa gtttatgctt
    12121 gcttacatga agaaacaaaa aaaattatga gaaacagaat ttactcacta ctgtgcaaat
    12181 tatatgttca actgagcaaa caagtcttca actacctctc ctcagagttg aatctgttcc
    12241 atgcttccta tagacacaaa agaacgataa ccactaagat aattcaaaga agataataaa
    12301 agagaatgga ccatgggaag aagaggggga ggtgaagctg gcgctgtcag gagctaactg
    12361 agatactacc atagatgact acccatccct ctaggcctta gataaccgga tacagtgact
    12421 ttgataggtt tgtggggtac agctatgact tgcatagctg cgtatggagg aaggaacttt
    12481 tgcgtgttag tatgttgacc cgtgtattac gcatgcgggt agatcatgtg ggtagagaca
    12541 tccaggtcaa gttctcgacc ttctcgtggg aggtgatcca gttcactata ggaccattcc
    12601 attcgagcat ggcactaagt acgccgtccc cattctggta atcatcatcc ctatcagggt
    12661 ttggagtgag tggtgacggt tattccccag gaatggactt cttattcaca gtcggtcaca
    12721 ttgggctact ccttgggtct tccgcttggc ccggtctgtt gggccgccat tgcgtgagtt
    12781 tcggccctgt gctgcgctgt gtcggcgatt ctcattgggg cctcacatct ggaaaccccg
    12841 acctattttg acggcaccat tggcggaagt tgctgggctt gtgcaccgtg aagtcctccg
    12901 cgggcctccc ccctacaaaa gacgataagc tccggcaagc agcattgatc aacgcaagga
    12961 tcggcgatat taacagagat acggctgatt actcttgttg gtgtggtatc gctaaactgc
    13021 gccgcggagc cttatggcaa agtcgtccgc gaaccattcc ggtagcgctt aaggtccata
    13081 gcacattcat cgcatcccgg cgtgcgttca atttgacgac cccttggcgc aaaggtgctg
    13141 gccacgtgct aaattaaagc ggctgcactg ctgtaaggtc cgttacggag agggcggcct
    13201 ggggagagca ctagcccatc gacctgtaca ggaacactct atattgttct cggacggaca
    13261 gattactaga gtgccgcttt cagcccccct gtcgtcgccg acgtctgtaa tatggcgttg
    13321 ttgtgattcc actctattga ggcattaact gatgcgtaag gagatctgga atgaactggc
    13381 ctatgtcact gaaactgtgc aaacacccaa tgtcgttagt gtaggttctg accgatacct
    13441 gcttcgttga gaactcacaa ttttacaact ggggacataa tccctacgcc catcatctac
    13501 tcgcgtccct gtggctccag ttcatgtaat gggagagtat cctccacaag atctagtgct
    13561 atggtggtat agtaagctcg tactgtggta gatgactccc tcttctctca ccaaccagtc
    13621 aacgccccgt accgatgctg aacaagtcga tgcaggccct tcttctctca ccaaccagtc
    13681 caatcccgag gcctgacccg acatatccca tcttctctca ccaaccagtc gtagctaact
    13741 atgttcccgt cctaatacct ctacgtgcaa gcagtataaa aacaaaatac ataaacataa
    13801 ctaaaatctc atatcgttgt aattgtcata tactagtggt aattaagtcc agttacactt
    13861 aaggttaaat ttgctgactc ttttttacct ttcatttctg aaaatggttg tgttgactag
    13921 caaggagaca aagcctagcg tcgctgaatt ttacgctgga aaaagtgttt ttattactgg
    13981 gggtactggg tttctcggta aggttttcat tgagaaactc ttgtattctt gtccagatat
    14041 agaaaacatt tacatgttga tcagggaaaa aaaaggttta tcagttagtg agaggatcaa
    14101 gcagtttctt gacgaccctc tgtttaccag gttgaaggat aagaggcctg ctgatcttga
    14161 gaaaatcgtc cttattcctg gcgacattac tgctcccgac ttgggtatta acagtgagaa
    14221 cgaaaaaatg cttatagaaa aggtgagtgt tatcatacac tcagcagcta ccgtcaagtt
    14281 caatgagcca ttgcctaccg cttggaagat caatgtcgag ggtacacgga tgatgttagc
    14341 acttagcaga cggatgaaga gaatagaggt tttcatccat atctctaccg cttacacaaa
    14401 tacaaacagg gaggtggttg atgaaatttt gtaccctgca cctgctgaca ttgaccaagt
    14461 tcaccagtac gtgaaagatg gtatctctga agaagatact gagaagatcc tcaatggaag
    14521 gcccaataca tatactttca ccaaggctct gaccgagcat ttagttgctg agaaccaagc
    14581 ttatgtgcca accattatcg ttaggccgag tgttgttgct gctataaagg acgagccact
    14641 taaaggatgg ctgggcaatt ggtttggcgc taccggattg acagttttta ccgcaaaggg
    14701 actaaacagg gtcatttatg gtcattcctc atacatagtt gatcttattc ctgtagatta
    14761 cgttgctaat ttagttatcg ccgcaggagc caagtcttca aaatctacag aattgaaagt
    14821 gtataattgc tgctcttcaa gctgcaaccc agtgaccatt gggacgttaa tgagtatgtt
    14881 cgctgacgac gctataaagc agaaaagtta tgcaatgccg ttaccgggtt ggtacatatt
    14941 taccaagtat aagtggcttg tgctactgtt gactttctta tttcaggtca ttcctgctta
    15001 tgttacagat ctctctaggc atctgattgg taaatcccca cgttatatca agctccaatc
    15061 cctggttaat cagactagat catctataga tttctttacc aaccacagct gggttatgaa
    15121 ggccgaccgg gtgagggagt tgtacgcatc actgtctccc gccgataaat acctattccc
    15181 gtgtgatcct accgatataa attggactca ttatatacag gattattgtt ggggtgtgag
    15241 gcacttcttg gaaaagaaat atcggtgagc ttctttgtgg ttattattta gcttctgtac
    15301 actaaattta tgatgcaaga agcgttgtac acaacatata gaagaagagt gcgaggtgaa
    15361 gcaagtagga gaaatgttag gaaagctcct atacaaaagg atggcatgtt gaagattagc
    15421 atctttttaa tcccaagttt aaatataaag catattttat gtaccacttt ctttatctgg
    15481 ggtttgtaat ccctttatat ctttatgcaa tctttacgtt agttaatatc tatctatcga
    15541 tattctagta tcttatacta tagatccaac tgaaccaaga aattatgaac cgtgtcttcc
    15601 agaaattcta ataatgatgg gagcaatata aatataagga tgtctttgac aataaaaggg
    15661 cggtggaaga gttatagtga aagcttctgg ccatcaaatg ggctctttgt cacatgattg
    15721 aactacttca ccacctaccg ttggtatcca tagtaaattg agattaaaaa aagttaaaac
    15781 aaactattca ttccatgtca ttttactttt caacttgcta tcttctaatt ataagtgggt
    15841 tgtggagaga gcactgttgg gtcagcaaag caaagtagat tatactctat aatattatga
    15901 tgattagtca actgctgctc acttacaacg atggaaatgg ggatgagggt aactttacgc
    15961 tgtcatgaga cgaattctga caggatatat tggcgggtaa acctaagaga aaagagcgtt
    16021 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
    16081 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
    16141 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg
    16201 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
    16261 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
    16321 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
    16381 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
    16441 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
    16501 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
    16561 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
    16621 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
    16681 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
    16741 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
    16801 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
    16861 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
    16921 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
    16981 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
    17041 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
    17101 acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
    17161 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
    17221 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
    17281 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
    17341 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
    17401 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
    17461 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
    17521 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
    17581 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
    17641 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
    17701 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
    17761 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
    17821 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
    17881 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
    17941 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
    18001 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
    18061 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
    18121 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
    18181 ataaatgagt
//
