LOCUS       sequence-378051-       19180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and
            ScATF1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 19180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB3898
FEATURES             Location/Qualifiers
     source          1..19180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(88..879)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    1665..1689
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1735..1987)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2027..2821)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2138..2157)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2748..2767
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2855..3034)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    14206..14230
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             15531..16160
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             16589..17662
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      17728..17922
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(18158..18180)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    18266..18406
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18321..18340
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18421..18438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18592..19180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18672..18691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgcattc taggtgatta tttgccgact accttggtga tctcgccttt
      121 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
      181 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
      241 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
      301 aatgcgggac aacgtaagca ctacatttcg ctcatcacca gcccagtcgg gcggcgagtt
      361 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
      421 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
      481 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
      541 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
      601 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
      661 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
      721 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
      781 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
      841 tagttgagtc gatacttcgg cgatcaccgc ttccctcata atgtttaact ttgttttagg
      901 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
      961 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata
     1021 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     1081 ccagttgcgt gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt
     1141 ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag
     1201 cagcgaagtc gaggcatttc tgtcctggct ggaacagaac ttattatttc cttcctcttt
     1261 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
     1321 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
     1381 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
     1441 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
     1501 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
     1561 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
     1621 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
     1681 ggtgtaaaca taacggatcc ggtctcagga gagcgatcag cttgcatgcc ggtcgatcta
     1741 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
     1801 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
     1861 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     1921 taatcatggc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     1981 aacgatctgc ttgactctag ctagagtccg aaccccagag tcccgctcag aagaactcgt
     2041 caagaaggcg atagaaggct atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2101 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2161 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2221 cattttccac catgatattc ggcaagcagg cgtcgccgtg ggtcacgacg agatcctcgc
     2281 cgtcgggcat ccgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     2341 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     2401 tgcggtgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     2461 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     2521 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     2581 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
     2641 ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     2701 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     2761 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     2821 tgcctcgatc gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg
     2881 gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga
     2941 cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac
     3001 ccgcggctca gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt
     3061 gtcccgcgtc atcggcgggg gtcataccgt gactccctta attctcatgt atctccgtca
     3121 ggagccctga ttttttcact actaatagta ctcacggcgc aataccagca cagcctagtc
     3181 tcgccagaat gctggtcagc atacgaaaga gcttaaggca ggccaattcc actgtcaggg
     3241 tcacttgggt gtttagcact accgacaggt acgctagtat gcgttcttcc taccagaggt
     3301 ctgtggccgc gtggtcaaaa gtgcggcttt cgtatttgct gctcgtgttt actctcacaa
     3361 acttgacctg cacgccaaag agatgcttct tgtggaactc gacaacgcaa caacgcgacg
     3421 gatctacgtc acagcgtgca tagtgaaaac ggagttgctg acgacgaaag cgacattggg
     3481 atctgtcagt tgtcattcgc gaaaaacatc cgtccccgag gcggacactg attgacgcgg
     3541 ttttgtagaa ggttagggga ataggttaga ttgagtggct taagaatgta aaatctggga
     3601 ttatagtgta gtaatctctg attaacggtg acggttttaa gacaggtgtt cgcaaaatca
     3661 agcggggtca tttcaacaga tattgctgat ggtttaggcg tacaatgccc tgaagaataa
     3721 ttaagaaaaa agcacccctc gtcgcctaga attacctacc gcggtccacc ataccttcga
     3781 ttatcgcgcc cactctccca ttagtcggca gaggtggttg tgttgcgata gcccagtatg
     3841 atattctaag gcgttacgct gatgaatatt ctacagagtt gccataggcg ttgaacgcta
     3901 cacggacgat acgaatttac gtatagagcg ggtcatcgaa aggttatact ctcgtagtta
     3961 acatctagcc cggccctatc agtacagcag tgccttgaat gacatactca tcattaaatt
     4021 ttctctacag ccaaacgacc aagtgcattt ccagggagcg cgatggagat tcattctctc
     4081 gccagcactg taataggcac taaaagagtg atgataatca tgagtgccgc gctaaggtgg
     4141 tgtcggaaca aagcggtctt acggtcagtc gtattccttc tcgagttccg tccagttgag
     4201 cgtgtcactc ccagtgtacc tgcaagccga gatggctgtg cttggagtca atcgcatgta
     4261 gcatggtcac cagacaccgg ggcaccagtt ttcacgccta aagcataaac gacgagcagt
     4321 catgaaagtc ttagtactgg acgtgccgtt tcactgcgaa tatgactccc tcttctctca
     4381 ccaaccagtc acgaccgctt cacgctaagg tgctggccac gtgctaacct tcttctctca
     4441 ccaaccagtc tggcgttgat gtggatcgac tctatagcca tcttctctca ccaaccagtc
     4501 cgaggccgtt cgtttcccgt cctaatacct ctacgtgcaa gcagtataaa aacaaaatac
     4561 ataaacataa ctaaaatctc atatcgttgt aattgtcata tactagtggt aattaagtcc
     4621 agttacactt aaggttaaat ttgctgactc ttttttacct ttcatttctg aaaatggttc
     4681 ctaataaggg ttcttctgat gtcttgtccg aacattctga gccacagttc actaagctga
     4741 tcgcccctca agccggacca aggaagtata agatcgtgta ccgaaactta ctgactttcg
     4801 gatattggca cctttctgct gtttatggct tgtacctttg tttcacttgt gctaaatggg
     4861 caacgatttt gtttgccttt tttctttacg tgatagctga aattgggatt actggtggag
     4921 ctcacagact ttgggcacat agaacttaca aggctaaact gcccctagaa attcttcttc
     4981 tcattatgaa ctccatagct tttcaagata ctgcatttac ttgggccagg gaccatagac
     5041 ttcaccacaa atactcagat actgatgcag atcctcacaa cgcaacccgg ggtttttttt
     5101 attcccacgt tgggtggttg ttggtcaaaa agcatccaga ggttaaggct agaggaaagt
     5161 acttgtctct cgatgactta aagaataacc cgctattgaa gttccaaaaa aaatacgcca
     5221 tactcgtaat tggaacctta tgttttttga tgcctacgtt cgtcccggta tacttttggg
     5281 gtgaaggcat ctctactgca tggaacatca atcttttacg atatgtcatg aacttaaata
     5341 tgactttcct agtcaactct gctgcccata tatttgggaa caagccgtat gataaatcaa
     5401 tagcttctgt gcaaaatata tcagtttccc tggctacatt tggggagggt ttccataatt
     5461 atcatcacac atatccctgg gattatcgtg cagcagaact tggaaacaat agattgaata
     5521 tgacaaccgc ctttattgat ttcttcgcct ggataggttg ggcatatgat ctaaagtctg
     5581 tgccacaaga ggctattgca aaacggtgcg caaaaactgg agatggtact gatatgtggg
     5641 gtaggaaacg ttgagcttaa acccaaccat acataataaa aaggcgagct acctcaccaa
     5701 catgttcttg gttcttgatg gctgcttgag gtttttcaac acaacatgag taaaatggtc
     5761 aataaatatg gatattctca tcttgcttga ttcgggcagg taggatatca gagaattcta
     5821 ggtatatttt gtagggaaaa aaaactagaa tctcttcagg atctggatcc taatacaagg
     5881 acatattaat attccatttt ataaactttg cacaagcaag atctaatttt atggaagaac
     5941 tgaaggctct cgcgtatcat ttgccaaaat aattaccatc ttttgtaatc atcttggaaa
     6001 ctagagataa tattgataat atccatagta ataataataa taagaagaac aaatatcatc
     6061 ataacacaat aatatagcct tttcatgtaa aatttcattt caatgaatag ccatgttagc
     6121 ccattttctt tcaggaaagt ttatgcttgc ttacatgaag aaacaaaaaa aattatgaga
     6181 aacagaattt actcactact gtgcaaatta tatgttcaac tgagcaaaca agtcttcaac
     6241 tacctctcct cagagttgaa tctgttccat gcttcctata gacacaaaag aacgataacc
     6301 actaagataa ttcaaagaag ataataaaag agaatggacc atgggaagaa gagggggagg
     6361 tgaagctggc gctgtcagga gctaactgag atactaccat agatgactac ccatccctct
     6421 aggccttaga taaccggata cagtgacttt gataggtttg tggggtacag ctatgacttg
     6481 catagctgcg tatggaggaa ggaacttttg cgtgttagta tgttgacccg tgtattacgc
     6541 atgcgggtag atcatgtggg tagagacatc caggtcaagt tctcgacctt ctcgtgggag
     6601 gtgatccagt tcactatagg accattccat tcgagcatgg cactaagtac gccgtcccca
     6661 ttctggtaat catcatccct atcagggttt ggagtgagtg gtgacggtta ttccccagga
     6721 atggacttct tattcacagt cggtcacatt gggctactcc ttgggtcttc cgcttggccc
     6781 ggtctgttgg gccgccattg cgtgagtttc ggccctgtgc tgcgctgtgt cggcgattct
     6841 cattggggcc tcacatctgg aaaccccgac ctattttgac ggcaccattg gcggaagttg
     6901 ctgggcttgt gcaccgtgaa gtcctccgcg ggcctccccc ctacaaaaga cgataagctc
     6961 cggcaagcag cattgatcaa cgcaaggatc ggcgatatta acagagatac ggctgattac
     7021 tcttgttggt gtggtatcgc taaactgcgc cgcggagcct tatggcaaag tcgtccgcga
     7081 accattccgg tagcgcttaa ggtccatagc acattcatcg catcccggcg tgcgttcaat
     7141 ttgacgaccc cttggcgcaa aggtgctggc cacgtgctaa attaaagcgg ctgcactgct
     7201 gtaaggtccg ttacggagag ggcggcctgg ggagagcact agcccatcga cctgtacagg
     7261 aacactctat attgttctcg gacggacaga ttactagagt gccgctttca gcccccctgt
     7321 cgtcgccgac gtctgtaata tggcgttgtt gtgattccac tctattgagg cattaactga
     7381 tgcgtaagga gatctggaat gaactggcct atgtcactga aactgtgcaa acacccaatg
     7441 tcgttagtgt aggttctgac cgatacctgc ttcgttgaga actcacaatt ttacaactgg
     7501 ggacataatc cctacgccca tcatctactc gcgtccctgt ggctccagtt catgtaatgg
     7561 gagagtatcc tccacaagat ctagtgctat ggtggtatag taagctcgta ctgtggtaga
     7621 tgactccctc ttctctcacc aaccagtcaa cgccccgtac cgatgctgaa caagtcgatg
     7681 caggcccttc ttctctcacc aaccagtcca atcccgaggc ctgacccgac atatcccatc
     7741 ttctctcacc aaccagtcgt agctaactat gttcccgtcc taatacctct acgtgcaagc
     7801 agtataaaaa caaaatacat aaacataact aaaatctcat atcgttgtaa ttgtcatata
     7861 ctagtggtaa ttaagtccag ttacacttaa ggttaaattt gctgactctt ttttaccttt
     7921 catttctgaa aatggttgtg ttgactagca aggagacaaa gcctagcgtc gctgaatttt
     7981 acgctggaaa aagtgttttt attactgggg gtactgggtt tctcggtaag gttttcattg
     8041 agaaactctt gtattcttgt ccagatatag aaaacattta catgttgatc agggaaaaaa
     8101 aaggtttatc agttagtgag aggatcaagc agtttcttga cgaccctctg tttaccaggt
     8161 tgaaggataa gaggcctgct gatcttgaga aaatcgtcct tattcctggc gacattactg
     8221 ctcccgactt gggtattaac agtgagaacg aaaaaatgct tatagaaaag gtgagtgtta
     8281 tcatacactc agcagctacc gtcaagttca atgagccatt gcctaccgct tggaagatca
     8341 atgtcgaggg tacacggatg atgttagcac ttagcagacg gatgaagaga atagaggttt
     8401 tcatccatat ctctaccgct tacacaaata caaacaggga ggtggttgat gaaattttgt
     8461 accctgcacc tgctgacatt gaccaagttc accagtacgt gaaagatggt atctctgaag
     8521 aagatactga gaagatcctc aatggaaggc ccaatacata tactttcacc aaggctctga
     8581 ccgagcattt agttgctgag aaccaagctt atgtgccaac cattatcgtt aggccgagtg
     8641 ttgttgctgc tataaaggac gagccactta aaggatggct gggcaattgg tttggcgcta
     8701 ccggattgac agtttttacc gcaaagggac taaacagggt catttatggt cattcctcat
     8761 acatagttga tcttattcct gtagattacg ttgctaattt agttatcgcc gcaggagcca
     8821 agtcttcaaa atctacagaa ttgaaagtgt ataattgctg ctcttcaagc tgcaacccag
     8881 tgaccattgg gacgttaatg agtatgttcg ctgacgacgc tataaagcag aaaagttatg
     8941 caatgccgtt accgggttgg tacatattta ccaagtataa gtggcttgtg ctactgttga
     9001 ctttcttatt tcaggtcatt cctgcttatg ttacagatct ctctaggcat ctgattggta
     9061 aatccccacg ttatatcaag ctccaatccc tggttaatca gactagatca tctatagatt
     9121 tctttaccaa ccacagctgg gttatgaagg ccgaccgggt gagggagttg tacgcatcac
     9181 tgtctcccgc cgataaatac ctattcccgt gtgatcctac cgatataaat tggactcatt
     9241 atatacagga ttattgttgg ggtgtgaggc acttcttgga aaagaaatat cggtgagctt
     9301 ctttgtggtt attatttagc ttctgtacac taaatttatg atgcaagaag cgttgtacac
     9361 aacatataga agaagagtgc gaggtgaagc aagtaggaga aatgttagga aagctcctat
     9421 acaaaaggat ggcatgttga agattagcat ctttttaatc ccaagtttaa atataaagca
     9481 tattttatgt accactttct ttatctgggg tttgtaatcc ctttatatct ttatgcaatc
     9541 tttacgttag ttaatatcta tctatcgata ttctagtatc ttatactata gatccaactg
     9601 aaccaagaaa ttatgaaccg tgtcttccag aaattctaat aatgatggga gcaatataaa
     9661 tataaggatg tctttgacaa taaaagggcg gtggaagagt tatagtgaaa gcttctggcc
     9721 atcaaatggg ctctttgtca catgattgaa ctacttcacc acctaccgtt ggtatccata
     9781 gtaaattgag attaaaaaaa gttaaaacaa actattcatt ccatgtcatt ttacttttca
     9841 acttgctatc ttctaattat aagtgggttg tggagagagc actgttgggt cagcaaagca
     9901 aagtagatta tactctataa tattatgatg attagtcaac tgctgctcac ttacaacgat
     9961 ggaaatgggg atgagggtaa ctttacgctg tcaagcgggg tttagccatt aacatcgtcg
    10021 tcaaagttcg atttctggag tgtgaaaaat tagggtttcg tcgataggag atgaaaagtc
    10081 gaaatttcgg tgcgagaaat caaaattgat ggaagatcgg cggcgaattt gagaggatag
    10141 tgagaagaag aagattaaga gatgatttga attgggaggg aaaatcgaat cgccagtgtt
    10201 tgttttaggg tttttaatct ccgggaattg aattttgggc gtcgtaaaac tgtaaagtgt
    10261 gtttatgtag taatctttat ctgagatgat acagaagaga ttctatccca gatacggtaa
    10321 atttgttttc ccctaggtcg gtggtcaaca ttgcattttt aagtcaactt ttaatatatt
    10381 ttagtttaat ttctataatt taaccttttt ggcattatct ttaggaaatt tttttgacgt
    10441 attttatttt acttattaaa agtgaaaaat cttaaacata ttaattaata tcttgcataa
    10501 ccaattgaca ttgtaatcat cacaattaaa tttgattaaa aatccacttt ctgcaaacaa
    10561 acaaagtttt ttttaataaa gtctataaca tatcgagtat aatagtatca atatagattt
    10621 tgatgataac aacaatagcc actcctgacg gagtgtttca tgtccaagcg aaaagccgct
    10681 ctacggaatg gatctacgtt actgcctgca taaggagatc ggtgtagcca aggacgaaag
    10741 cgaccctagg ttctaaccgt cgactttggc ggaaaggttt cactcaggaa gcagacactg
    10801 attgacacgg tttagcagaa cgtttgagga ctaggtcaaa ttgagtggtt taatatcggc
    10861 atgtctggct ttaaaattca gtatagtgcg ctgatcggag atgaattaaa aacacgagtt
    10921 cccaaaacca ggcgggctcg ccacgtcggc taatcctggt acattatgtg aacaatgttc
    10981 tgaagaaaat ttgtgaaaga aggacgggtc atcgcctact attagcaaca acggtcggcc
    11041 acaccttcca ttgtcgtggc cacgctcgga ttacacggca gaggtgcttg tgttccgaca
    11101 ggctagcata ttatcctaag gcgttacccc aatcgtttac cgtcggattt gctatagccc
    11161 ctgaacgcta catgtacgaa accatgttat gtatgcacta ggtcaacaat aggacatagc
    11221 cttgtagtta acacgtagcc cggtcgtata agtacagtag acccttcgcc ggcatcctat
    11281 ttattaagtt atttctacag caaaacgatc atatgcagat ccgcagtggg cggtagacac
    11341 acgtccaccc ggctgctctg taacagggac taaagaggtg atgattatcg tgtgtgcccc
    11401 gttatggtcg tgttcgatca gagcgccctt gcgagcagtc gtatgctttc tcgaattccg
    11461 agcggttaag cgtgacagtc ccagcgaacc cacaaaacgt gatcgcagtc catgcgatca
    11521 tacgcaagaa ggaaggtccc catacaccga cgcaccagtt tacacgccgt atgcataaac
    11581 gagctgcaca aacgagagtg tttgaactgg acctctagtt cctctacaaa gaacaccttg
    11641 acctgttgcg aagttgcctt gcctagatgc aatgtcggac gtatcacttt tgcctcaacg
    11701 actgctgctt tcgctgtaac ccaagacaga caacagtaac cgccttttga aggcaagtcc
    11761 tccgcctgtg actaactgtg ccaaatcgtc ttccaaaccc cttatccaat ttaactcacc
    11821 aaattattgc gatacagacc ctaatttcac atcatatgac actaattgcc tctgccaaaa
    11881 ttctgtcctc aatgactccc tcttctctca ccaaccagtc agttgggttc acccgggtcg
    11941 gacctgagtc gaccaagcct tcttctctca ccaaccagtc gcgaccgcag aacttacgct
    12001 cgagggtccg tcttctctca ccaaccagtc tccccggtta tctctcccgt cctaatacct
    12061 ctacgtgcaa gcagtataaa aacaaaatac ataaacataa ctaaaatctc atatcgttgt
    12121 aattgtcata tactagtggt aattaagtcc agttacactt aaggttaaat ttgctgactc
    12181 ttttttacct ttcatttctg aaaatgaatg aaattgacga aaagaaccaa gcaccggtcc
    12241 agcaagaatg cctcaaagaa atgatccaaa acggtcatgc ccgacgaatg gggagtgtcg
    12301 aggaccttta tgtagctctc aatagacaaa acctgtaccg aaacttttgc acatatggag
    12361 aactttcaga ctattgtacg cgagatcaac tcaccctggc tctccgtgaa atttgtttga
    12421 agaatccgac cctgcttcac atcgtgttac ctacgcgttg gccaaaccac gaaaactact
    12481 atcgttcctc cgaatattac tcaagaccgc atcccgttca cgattacatc tccgttttgc
    12541 aagaattaaa attaagcggg gtcgtactta atgaacaacc tgagtattca gctgttatga
    12601 agcaaatttt agaagaattc aaaaatagta agggttctta cacagctaag atatttaaac
    12661 ttactacaac tctcacgatt ccttacttcg gccctacagg accgtcatgg cgattaattt
    12721 gtcttcccga agagcatacg gagaaatgga agaaattcat attcgttagc aatcactgca
    12781 tgagtgatgg acgatctagt atacactttt ttcatgatct tagagatgag cttaataaca
    12841 taaagactcc tccaaaaaag ctcgactaca tcttcaagta cgaggaggat tatcaactct
    12901 taagaaagct ccccgagcca atagaaaagg tgatcgactt tcgtccccca tacctgttta
    12961 taccgaagag cctcctttct ggatttatct ataaccacct gagattctca tcaaagggag
    13021 tctgtatgag gatggacgat gttgaaaaga cagatgatgt tgtcacggaa attataaaca
    13081 taagcccaac tgaattccag gccataaaag caaatatcaa atcaaacatc cagggaaagt
    13141 gcacgataac acctttttta cacgtttgtt ggtttgtgag cttacacaaa tggggtaaat
    13201 ttttcaagcc cctcaatttc gagtggctta cagatatatt catacctgcc gattgcagat
    13261 cacaactccc cgatgatgac gagatgcgtc agatgtatcg atatggtgcc aatgtcgggt
    13321 tcatagactt cactccatgg atcagcgagt tcgacatgaa tgataataag gagaatttct
    13381 ggccgttaat tgagcattac cacgaggtaa ttagtgaagc cttaaggaat aaaaagcacc
    13441 tccatggctt agggttcaac atccaaggtt tcgttcagaa gtatgtcaat atcgataaag
    13501 tcatgtgcga tagggctatc ggcaaaagac gaggtggtac gctgttatct aatgtggggt
    13561 tatttaatca gctggaggag ccagacgcca aatacagtat ctgtgatttg gcatttggcc
    13621 aatttcaggg atcatggcat caagctttct ccttgggagt gtgttctact aatgttaagg
    13681 gcatgaacat agtggttgcc tccacgaaaa atgtcgtagg cagtcaagag tctttagaag
    13741 agttgtgttc catttataag gctcttctgc tgggaccata ggcttacgaa aatatagttg
    13801 aaacagagca gagatcatgg tgttagcctt ctatggctga agaaaaatag ttgaaaaaat
    13861 gtgtgtgtct agtgttttag tgtgtctgtc tgttgaaaac agggaaaaaa atgtgttctt
    13921 ttttccctgt aataagaaat atagtaatgg agtctttatg tgtaagtaac cagtaaaatg
    13981 gtttgtgtaa acatgaatat ctctgcatct gttaatttta acatataagt ttggatatgt
    14041 gttgtggttt caactaattc aagtacttcc ttgataatgt tttcctgttt ctaggctgca
    14101 aaactatagt aaatctttta ggttttggta aaacctaatt ccattgggtg tggttggaga
    14161 gcatatagtt tttgcgacgc tctccgctgt catgagaccg gatcctgaca ggatatattg
    14221 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
    14281 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
    14341 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
    14401 gcagccgtca tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
    14461 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
    14521 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
    14581 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
    14641 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
    14701 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
    14761 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
    14821 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
    14881 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
    14941 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
    15001 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
    15061 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
    15121 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
    15181 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
    15241 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
    15301 gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg tgcggctgca tgaaatcctg
    15361 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
    15421 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
    15481 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
    15541 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
    15601 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
    15661 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
    15721 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
    15781 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
    15841 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
    15901 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
    15961 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
    16021 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
    16081 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
    16141 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
    16201 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
    16261 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
    16321 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
    16381 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
    16441 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
    16501 gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca
    16561 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
    16621 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
    16681 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
    16741 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
    16801 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
    16861 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
    16921 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
    16981 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
    17041 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
    17101 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
    17161 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
    17221 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
    17281 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
    17341 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
    17401 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
    17461 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
    17521 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
    17581 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
    17641 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
    17701 ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg tacattggga acccaaagcc
    17761 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
    17821 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
    17881 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
    17941 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
    18001 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
    18061 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
    18121 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    18181 tgacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    18241 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
    18301 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    18361 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
    18421 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    18481 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    18541 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    18601 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    18661 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    18721 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    18781 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    18841 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    18901 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    18961 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    19021 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    19081 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    19141 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
//
