LOCUS       sequence-378052-       19180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for dCasEV2.1 activated expression of HarFAR, ATF1 and Atr 
            delta11..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 19180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB4366
FEATURES             Location/Qualifiers
     source          1..19180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1048..1072
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1118..1370)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1410..2204)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1521..1540)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2131..2150
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2238..2417)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13589..13613
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14914..15543
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             15972..17045
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      17111..17305
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(17541..17563)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17649..17789
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17704..17723
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(17804..17821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17975..18563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18055..18074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(18651..19180,1..262))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
       61 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
      121 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
      181 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
      241 cggcgatcac cgcttccctc ataatgttta actttgtttt agggcgactg ccctgctgcg
      301 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
      361 tggatgcccg aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg
      421 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
      481 tacgctactt gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgcct
      541 tcatccgttt ccacggtgtg cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat
      601 ttctgtcctg gctggaacag aacttattat ttccttcctc ttttctacag tatttaaaga
      661 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
      721 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
      781 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
      841 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
      901 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
      961 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     1021 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acataacgga
     1081 tccggtctca ggagagcgat cagcttgcat gccggtcgat ctagtaacat agatgacacc
     1141 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     1201 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     1261 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat ggcaagaccg
     1321 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc
     1381 tagctagagt ccgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     1441 gctatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     1501 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     1561 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     1621 ttcggcaagc aggcgtcgcc gtgggtcacg acgagatcct cgccgtcggg catccgcgcc
     1681 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     1741 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcggtg tttcgcttgg
     1801 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     1861 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     1921 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     1981 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca
     2041 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     2101 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     2161 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga
     2221 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     2281 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     2341 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc
     2401 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     2461 ggggtcatac cgtgactccc ttaattctca tgtatctccg tcaggagcta actgagatac
     2521 taccatagat gactacccat ccctctaggc cttagataac cggatacagt gactttgata
     2581 ggtttgtggg gtacagctat gacttgcata gctgcgtatg gaggaaggaa cttttgcgtg
     2641 ttagtatgtt gacccgtgta ttacgcatgc gggtagatca tgtgggtaga gacatccagg
     2701 tcaagttctc gaccttctcg tgggaggtga tccagttcac tataggacca ttccattcga
     2761 gcatggcact aagtacgccg tccccattct ggtaatcatc atccctatca gggtttggag
     2821 tgagtggtga cggttattcc ccaggaatgg acttcttatt cacagtcggt cacattgggc
     2881 tactccttgg gtcttccgct tggcccggtc tgttgggccg ccattgcgtg agtttcggcc
     2941 ctgtgctgcg ctgtgtcggc gattctcatt ggggcctcac atctggaaac cccgacctat
     3001 tttgacggca ccattggcgg aagttgctgg gcttgtgcac cgtgaagtcc tccgcgggcc
     3061 tcccccctac aaaagacgat aagctccggc aagcagcatt gatcaacgca aggatcggcg
     3121 atattaacag agatacggct gattactctt gttggtgtgg tatcgctaaa ctgcgccgcg
     3181 gagccttatg gcaaagtcgt ccgcgaacca ttccggtagc gcttaaggtc catagcacat
     3241 tcatcgcatc ccggcgtgcg ttcaatttga cgaccccttg gcgcaaaggt gctggccacg
     3301 tgctaaatta aagcggctgc actgctgtaa ggtccgttac ggagagggcg gcctggggag
     3361 agcactagcc catcgacctg tacaggaaca ctctatattg ttctcggacg gacagattac
     3421 tagagtgccg ctttcagccc ccctgtcgtc gccgacgtct gtaatatggc gttgttgtga
     3481 ttccactcta ttgaggcatt aactgatgcg taaggagatc tggaatgaac tggcctatgt
     3541 cactgaaact gtgcaaacac ccaatgtcgt tagtgtaggt tctgaccgat acctgcttcg
     3601 ttgagaactc acaattttac aactggggac ataatcccta cgcccatcat ctactcgcgt
     3661 ccctgtggct ccagttcatg taatgggaga gtatcctcca caagatctag tgctatggtg
     3721 gtatagtaag ctcgtactgt ggtagatgac tccctcttct ctcaccaacc agtcaacgcc
     3781 ccgtaccgat gctgaacaag tcgatgcagg cccttcttct ctcaccaacc agtccaatcc
     3841 cgaggcctga cccgacatat cccatcttct ctcaccaacc agtcgtagct aactatgttc
     3901 ccgtcctaat acctctacgt gcaagcagta taaaaacaaa atacataaac ataactaaaa
     3961 tctcatatcg ttgtaattgt catatactag tggtaattaa gtccagttac acttaaggtt
     4021 aaatttgctg actctttttt acctttcatt tctgaaaatg gttgtgttga ctagcaagga
     4081 gacaaagcct agcgtcgctg aattttacgc tggaaaaagt gtttttatta ctgggggtac
     4141 tgggtttctc ggtaaggttt tcattgagaa actcttgtat tcttgtccag atatagaaaa
     4201 catttacatg ttgatcaggg aaaaaaaagg tttatcagtt agtgagagga tcaagcagtt
     4261 tcttgacgac cctctgttta ccaggttgaa ggataagagg cctgctgatc ttgagaaaat
     4321 cgtccttatt cctggcgaca ttactgctcc cgacttgggt attaacagtg agaacgaaaa
     4381 aatgcttata gaaaaggtga gtgttatcat acactcagca gctaccgtca agttcaatga
     4441 gccattgcct accgcttgga agatcaatgt cgagggtaca cggatgatgt tagcacttag
     4501 cagacggatg aagagaatag aggttttcat ccatatctct accgcttaca caaatacaaa
     4561 cagggaggtg gttgatgaaa ttttgtaccc tgcacctgct gacattgacc aagttcacca
     4621 gtacgtgaaa gatggtatct ctgaagaaga tactgagaag atcctcaatg gaaggcccaa
     4681 tacatatact ttcaccaagg ctctgaccga gcatttagtt gctgagaacc aagcttatgt
     4741 gccaaccatt atcgttaggc cgagtgttgt tgctgctata aaggacgagc cacttaaagg
     4801 atggctgggc aattggtttg gcgctaccgg attgacagtt tttaccgcaa agggactaaa
     4861 cagggtcatt tatggtcatt cctcatacat agttgatctt attcctgtag attacgttgc
     4921 taatttagtt atcgccgcag gagccaagtc ttcaaaatct acagaattga aagtgtataa
     4981 ttgctgctct tcaagctgca acccagtgac cattgggacg ttaatgagta tgttcgctga
     5041 cgacgctata aagcagaaaa gttatgcaat gccgttaccg ggttggtaca tatttaccaa
     5101 gtataagtgg cttgtgctac tgttgacttt cttatttcag gtcattcctg cttatgttac
     5161 agatctctct aggcatctga ttggtaaatc cccacgttat atcaagctcc aatccctggt
     5221 taatcagact agatcatcta tagatttctt taccaaccac agctgggtta tgaaggccga
     5281 ccgggtgagg gagttgtacg catcactgtc tcccgccgat aaatacctat tcccgtgtga
     5341 tcctaccgat ataaattgga ctcattatat acaggattat tgttggggtg tgaggcactt
     5401 cttggaaaag aaatatcggt gagcttcttt gtggttatta tttagcttct gtacactaaa
     5461 tttatgatgc aagaagcgtt gtacacaaca tatagaagaa gagtgcgagg tgaagcaagt
     5521 aggagaaatg ttaggaaagc tcctatacaa aaggatggca tgttgaagat tagcatcttt
     5581 ttaatcccaa gtttaaatat aaagcatatt ttatgtacca ctttctttat ctggggtttg
     5641 taatcccttt atatctttat gcaatcttta cgttagttaa tatctatcta tcgatattct
     5701 agtatcttat actatagatc caactgaacc aagaaattat gaaccgtgtc ttccagaaat
     5761 tctaataatg atgggagcaa tataaatata aggatgtctt tgacaataaa agggcggtgg
     5821 aagagttata gtgaaagctt ctggccatca aatgggctct ttgtcacatg attgaactac
     5881 ttcaccacct accgttggta tccatagtaa attgagatta aaaaaagtta aaacaaacta
     5941 ttcattccat gtcattttac ttttcaactt gctatcttct aattataagt gggttgtgga
     6001 gagagcactg ttgggtcagc aaagcaaagt agattatact ctataatatt atgatgatta
     6061 gtcaactgct gctcacttac aacgatggaa atggggatga gggtaacttt acgctgtcaa
     6121 gcggggttta gccattaaca tcgtcgtcaa agttcgattt ctggagtgtg aaaaattagg
     6181 gtttcgtcga taggagatga aaagtcgaaa tttcggtgcg agaaatcaaa attgatggaa
     6241 gatcggcggc gaatttgaga ggatagtgag aagaagaaga ttaagagatg atttgaattg
     6301 ggagggaaaa tcgaatcgcc agtgtttgtt ttagggtttt taatctccgg gaattgaatt
     6361 ttgggcgtcg taaaactgta aagtgtgttt atgtagtaat ctttatctga gatgatacag
     6421 aagagattct atcccagata cggtaaattt gttttcccct aggtcggtgg tcaacattgc
     6481 atttttaagt caacttttaa tatattttag tttaatttct ataatttaac ctttttggca
     6541 ttatctttag gaaatttttt tgacgtattt tattttactt attaaaagtg aaaaatctta
     6601 aacatattaa ttaatatctt gcataaccaa ttgacattgt aatcatcaca attaaatttg
     6661 attaaaaatc cactttctgc aaacaaacaa agtttttttt aataaagtct ataacatatc
     6721 gagtataata gtatcaatat agattttgat gataacaaca atagccactc ctgacggagt
     6781 gtttcatgtc caagcgaaaa gccgctctac ggaatggatc tacgttactg cctgcataag
     6841 gagatcggtg tagccaagga cgaaagcgac cctaggttct aaccgtcgac tttggcggaa
     6901 aggtttcact caggaagcag acactgattg acacggttta gcagaacgtt tgaggactag
     6961 gtcaaattga gtggtttaat atcggcatgt ctggctttaa aattcagtat agtgcgctga
     7021 tcggagatga attaaaaaca cgagttccca aaaccaggcg ggctcgccac gtcggctaat
     7081 cctggtacat tatgtgaaca atgttctgaa gaaaatttgt gaaagaagga cgggtcatcg
     7141 cctactatta gcaacaacgg tcggccacac cttccattgt cgtggccacg ctcggattac
     7201 acggcagagg tgcttgtgtt ccgacaggct agcatattat cctaaggcgt taccccaatc
     7261 gtttaccgtc ggatttgcta tagcccctga acgctacatg tacgaaacca tgttatgtat
     7321 gcactaggtc aacaatagga catagccttg tagttaacac gtagcccggt cgtataagta
     7381 cagtagaccc ttcgccggca tcctatttat taagttattt ctacagcaaa acgatcatat
     7441 gcagatccgc agtgggcggt agacacacgt ccacccggct gctctgtaac agggactaaa
     7501 gaggtgatga ttatcgtgtg tgccccgtta tggtcgtgtt cgatcagagc gcccttgcga
     7561 gcagtcgtat gctttctcga attccgagcg gttaagcgtg acagtcccag cgaacccaca
     7621 aaacgtgatc gcagtccatg cgatcatacg caagaaggaa ggtccccata caccgacgca
     7681 ccagtttaca cgccgtatgc ataaacgagc tgcacaaacg agagtgtttg aactggacct
     7741 ctagttcctc tacaaagaac accttgacct gttgcgaagt tgccttgcct agatgcaatg
     7801 tcggacgtat cacttttgcc tcaacgactg ctgctttcgc tgtaacccaa gacagacaac
     7861 agtaaccgcc ttttgaaggc aagtcctccg cctgtgacta actgtgccaa atcgtcttcc
     7921 aaacccctta tccaatttaa ctcaccaaat tattgcgata cagaccctaa tttcacatca
     7981 tatgacacta attgcctctg ccaaaattct gtcctcaatg actccctctt ctctcaccaa
     8041 ccagtcagtt gggttcaccc gggtcggacc tgagtcgacc aagccttctt ctctcaccaa
     8101 ccagtcgcga ccgcagaact tacgctcgag ggtccgtctt ctctcaccaa ccagtctccc
     8161 cggttatctc tcccgtccta atacctctac gtgcaagcag tataaaaaca aaatacataa
     8221 acataactaa aatctcatat cgttgtaatt gtcatatact agtggtaatt aagtccagtt
     8281 acacttaagg ttaaatttgc tgactctttt ttacctttca tttctgaaaa tgaatgaaat
     8341 tgacgaaaag aaccaagcac cggtccagca agaatgcctc aaagaaatga tccaaaacgg
     8401 tcatgcccga cgaatgggga gtgtcgagga cctttatgta gctctcaata gacaaaacct
     8461 gtaccgaaac ttttgcacat atggagaact ttcagactat tgtacgcgag atcaactcac
     8521 cctggctctc cgtgaaattt gtttgaagaa tccgaccctg cttcacatcg tgttacctac
     8581 gcgttggcca aaccacgaaa actactatcg ttcctccgaa tattactcaa gaccgcatcc
     8641 cgttcacgat tacatctccg ttttgcaaga attaaaatta agcggggtcg tacttaatga
     8701 acaacctgag tattcagctg ttatgaagca aattttagaa gaattcaaaa atagtaaggg
     8761 ttcttacaca gctaagatat ttaaacttac tacaactctc acgattcctt acttcggccc
     8821 tacaggaccg tcatggcgat taatttgtct tcccgaagag catacggaga aatggaagaa
     8881 attcatattc gttagcaatc actgcatgag tgatggacga tctagtatac acttttttca
     8941 tgatcttaga gatgagctta ataacataaa gactcctcca aaaaagctcg actacatctt
     9001 caagtacgag gaggattatc aactcttaag aaagctcccc gagccaatag aaaaggtgat
     9061 cgactttcgt cccccatacc tgtttatacc gaagagcctc ctttctggat ttatctataa
     9121 ccacctgaga ttctcatcaa agggagtctg tatgaggatg gacgatgttg aaaagacaga
     9181 tgatgttgtc acggaaatta taaacataag cccaactgaa ttccaggcca taaaagcaaa
     9241 tatcaaatca aacatccagg gaaagtgcac gataacacct tttttacacg tttgttggtt
     9301 tgtgagctta cacaaatggg gtaaattttt caagcccctc aatttcgagt ggcttacaga
     9361 tatattcata cctgccgatt gcagatcaca actccccgat gatgacgaga tgcgtcagat
     9421 gtatcgatat ggtgccaatg tcgggttcat agacttcact ccatggatca gcgagttcga
     9481 catgaatgat aataaggaga atttctggcc gttaattgag cattaccacg aggtaattag
     9541 tgaagcctta aggaataaaa agcacctcca tggcttaggg ttcaacatcc aaggtttcgt
     9601 tcagaagtat gtcaatatcg ataaagtcat gtgcgatagg gctatcggca aaagacgagg
     9661 tggtacgctg ttatctaatg tggggttatt taatcagctg gaggagccag acgccaaata
     9721 cagtatctgt gatttggcat ttggccaatt tcagggatca tggcatcaag ctttctcctt
     9781 gggagtgtgt tctactaatg ttaagggcat gaacatagtg gttgcctcca cgaaaaatgt
     9841 cgtaggcagt caagagtctt tagaagagtt gtgttccatt tataaggctc ttctgctggg
     9901 accataggct tacgaaaata tagttgaaac agagcagaga tcatggtgtt agccttctat
     9961 ggctgaagaa aaatagttga aaaaatgtgt gtgtctagtg ttttagtgtg tctgtctgtt
    10021 gaaaacaggg aaaaaaatgt gttctttttt ccctgtaata agaaatatag taatggagtc
    10081 tttatgtgta agtaaccagt aaaatggttt gtgtaaacat gaatatctct gcatctgtta
    10141 attttaacat ataagtttgg atatgtgttg tggtttcaac taattcaagt acttccttga
    10201 taatgttttc ctgtttctag gctgcaaaac tatagtaaat cttttaggtt ttggtaaaac
    10261 ctaattccat tgggtgtggt tggagagcat atagtttttg cgacgctctc cgctgtcagg
    10321 agccctgatt ttttcactac taatagtact cacggcgcaa taccagcaca gcctagtctc
    10381 gccagaatgc tggtcagcat acgaaagagc ttaaggcagg ccaattccac tgtcagggtc
    10441 acttgggtgt ttagcactac cgacaggtac gctagtatgc gttcttccta ccagaggtct
    10501 gtggccgcgt ggtcaaaagt gcggctttcg tatttgctgc tcgtgtttac tctcacaaac
    10561 ttgacctgca cgccaaagag atgcttcttg tggaactcga caacgcaaca acgcgacgga
    10621 tctacgtcac agcgtgcata gtgaaaacgg agttgctgac gacgaaagcg acattgggat
    10681 ctgtcagttg tcattcgcga aaaacatccg tccccgaggc ggacactgat tgacgcggtt
    10741 ttgtagaagg ttaggggaat aggttagatt gagtggctta agaatgtaaa atctgggatt
    10801 atagtgtagt aatctctgat taacggtgac ggttttaaga caggtgttcg caaaatcaag
    10861 cggggtcatt tcaacagata ttgctgatgg tttaggcgta caatgccctg aagaataatt
    10921 aagaaaaaag cacccctcgt cgcctagaat tacctaccgc ggtccaccat accttcgatt
    10981 atcgcgccca ctctcccatt agtcggcaga ggtggttgtg ttgcgatagc ccagtatgat
    11041 attctaaggc gttacgctga tgaatattct acagagttgc cataggcgtt gaacgctaca
    11101 cggacgatac gaatttacgt atagagcggg tcatcgaaag gttatactct cgtagttaac
    11161 atctagcccg gccctatcag tacagcagtg ccttgaatga catactcatc attaaatttt
    11221 ctctacagcc aaacgaccaa gtgcatttcc agggagcgcg atggagattc attctctcgc
    11281 cagcactgta ataggcacta aaagagtgat gataatcatg agtgccgcgc taaggtggtg
    11341 tcggaacaaa gcggtcttac ggtcagtcgt attccttctc gagttccgtc cagttgagcg
    11401 tgtcactccc agtgtacctg caagccgaga tggctgtgct tggagtcaat cgcatgtagc
    11461 atggtcacca gacaccgggg caccagtttt cacgcctaaa gcataaacga cgagcagtca
    11521 tgaaagtctt agtactggac gtgccgtttc actgcgaata tgactccctc ttctctcacc
    11581 aaccagtcac gaccgcttca cgctaaggtg ctggccacgt gctaaccttc ttctctcacc
    11641 aaccagtctg gcgttgatgt ggatcgactc tatagccatc ttctctcacc aaccagtccg
    11701 aggccgttcg tttcccgtcc taatacctct acgtgcaagc agtataaaaa caaaatacat
    11761 aaacataact aaaatctcat atcgttgtaa ttgtcatata ctagtggtaa ttaagtccag
    11821 ttacacttaa ggttaaattt gctgactctt ttttaccttt catttctgaa aatggttcct
    11881 aataagggtt cttctgatgt cttgtccgaa cattctgagc cacagttcac taagctgatc
    11941 gcccctcaag ccggaccaag gaagtataag atcgtgtacc gaaacttact gactttcgga
    12001 tattggcacc tttctgctgt ttatggcttg tacctttgtt tcacttgtgc taaatgggca
    12061 acgattttgt ttgccttttt tctttacgtg atagctgaaa ttgggattac tggtggagct
    12121 cacagacttt gggcacatag aacttacaag gctaaactgc ccctagaaat tcttcttctc
    12181 attatgaact ccatagcttt tcaagatact gcatttactt gggccaggga ccatagactt
    12241 caccacaaat actcagatac tgatgcagat cctcacaacg caacccgggg ttttttttat
    12301 tcccacgttg ggtggttgtt ggtcaaaaag catccagagg ttaaggctag aggaaagtac
    12361 ttgtctctcg atgacttaaa gaataacccg ctattgaagt tccaaaaaaa atacgccata
    12421 ctcgtaattg gaaccttatg ttttttgatg cctacgttcg tcccggtata cttttggggt
    12481 gaaggcatct ctactgcatg gaacatcaat cttttacgat atgtcatgaa cttaaatatg
    12541 actttcctag tcaactctgc tgcccatata tttgggaaca agccgtatga taaatcaata
    12601 gcttctgtgc aaaatatatc agtttccctg gctacatttg gggagggttt ccataattat
    12661 catcacacat atccctggga ttatcgtgca gcagaacttg gaaacaatag attgaatatg
    12721 acaaccgcct ttattgattt cttcgcctgg ataggttggg catatgatct aaagtctgtg
    12781 ccacaagagg ctattgcaaa acggtgcgca aaaactggag atggtactga tatgtggggt
    12841 aggaaacgtt gagcttaaac ccaaccatac ataataaaaa ggcgagctac ctcaccaaca
    12901 tgttcttggt tcttgatggc tgcttgaggt ttttcaacac aacatgagta aaatggtcaa
    12961 taaatatgga tattctcatc ttgcttgatt cgggcaggta ggatatcaga gaattctagg
    13021 tatattttgt agggaaaaaa aactagaatc tcttcaggat ctggatccta atacaaggac
    13081 atattaatat tccattttat aaactttgca caagcaagat ctaattttat ggaagaactg
    13141 aaggctctcg cgtatcattt gccaaaataa ttaccatctt ttgtaatcat cttggaaact
    13201 agagataata ttgataatat ccatagtaat aataataata agaagaacaa atatcatcat
    13261 aacacaataa tatagccttt tcatgtaaaa tttcatttca atgaatagcc atgttagccc
    13321 attttctttc aggaaagttt atgcttgctt acatgaagaa acaaaaaaaa ttatgagaaa
    13381 cagaatttac tcactactgt gcaaattata tgttcaactg agcaaacaag tcttcaacta
    13441 cctctcctca gagttgaatc tgttccatgc ttcctataga cacaaaagaa cgataaccac
    13501 taagataatt caaagaagat aataaaagag aatggaccat gggaagaaga gggggaggtg
    13561 aagctggcgc tgtcatgaga ccggatcctg acaggatata ttggcgggta aacctaagag
    13621 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
    13681 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
    13741 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcatctgaaa
    13801 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
    13861 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
    13921 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
    13981 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
    14041 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
    14101 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
    14161 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
    14221 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
    14281 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
    14341 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
    14401 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
    14461 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
    14521 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
    14581 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
    14641 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
    14701 gccgcccgcg cacctctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
    14761 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
    14821 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
    14881 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
    14941 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
    15001 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
    15061 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
    15121 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
    15181 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
    15241 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
    15301 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
    15361 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
    15421 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
    15481 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
    15541 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
    15601 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
    15661 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
    15721 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
    15781 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
    15841 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
    15901 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
    15961 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
    16021 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
    16081 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
    16141 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
    16201 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
    16261 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
    16321 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
    16381 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
    16441 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
    16501 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
    16561 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
    16621 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
    16681 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
    16741 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
    16801 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
    16861 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
    16921 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
    16981 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
    17041 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
    17101 gactctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
    17161 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
    17221 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
    17281 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
    17341 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
    17401 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
    17461 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
    17521 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggtgacggt cacagcttgt
    17581 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
    17641 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
    17701 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    17761 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
    17821 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    17881 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    17941 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    18001 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    18061 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    18121 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    18181 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    18241 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    18301 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    18361 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    18421 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    18481 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    18541 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    18601 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtga ttatttgccg
    18661 actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc
    18721 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
    18781 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
    18841 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatca
    18901 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
    18961 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
    19021 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
    19081 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
    19141 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
//
