LOCUS       sequence-378053-       19180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and
            SpATF1-2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 19180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB4356
FEATURES             Location/Qualifiers
     source          1..19180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      70..264
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(500..522)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    608..748
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     663..682
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(763..780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(934..1522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1014..1033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1610..2401)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    3187..3211
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3257..3509)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3549..4343)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3660..3679)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4270..4289
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4377..4556)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    15728..15752
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             17053..17682
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(18111..19180,1..4)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
       61 actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
      121 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
      181 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
      241 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
      301 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
      361 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
      421 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
      481 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc
      541 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      601 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
      661 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
      721 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
      781 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      841 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      901 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      961 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1021 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1081 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1141 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1201 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1261 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1321 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     1381 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1441 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1501 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1561 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat tatttgccga
     1621 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg
     1681 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct
     1741 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
     1801 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcac
     1861 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
     1921 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
     1981 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
     2041 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
     2101 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
     2161 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
     2221 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
     2281 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
     2341 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
     2401 taatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
     2461 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     2521 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     2581 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct
     2641 tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc
     2701 gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg ctggaacaga
     2761 acttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
     2821 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
     2881 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
     2941 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
     3001 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
     3061 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
     3121 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
     3181 ggctggtggc aggatatatt gtggtgtaaa cataacggat ccggtctcag gagagcgatc
     3241 agcttgcatg ccggtcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     3301 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     3361 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     3421 taattcaaca gaaattatat gataatcatg gcaagaccgg caacaggatt caatcttaag
     3481 aaactttatt gccaaatgtt tgaacgatct gcttgactct agctagagtc cgaaccccag
     3541 agtcccgctc agaagaactc gtcaagaagg cgatagaagg ctatgcgctg cgaatcggga
     3601 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     3661 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     3721 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcgtcgccg
     3781 tgggtcacga cgagatcctc gccgtcgggc atccgcgcct tgagcctggc gaacagttcg
     3841 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     3901 atccgagtac gtgctcgctc gatgcggtgt ttcgcttggt ggtcgaatgg gcaggtagcc
     3961 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     4021 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     4081 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     4141 gatagccgcg ctgcctcgtc ttggagttca ttcagggcac cggacaggtc ggtcttgaca
     4201 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     4261 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     4321 tgcaatccat cttgttcaat catgcctcga tcgagttgag agtgaatatg agactctaat
     4381 tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct
     4441 agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct
     4501 tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg cggttctgtc
     4561 agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcatacc gtgactccct
     4621 taattctcat gtatctccgt caggagccct gattttttca ctactaatag tactcacggc
     4681 gcaataccag cacagcctag tctcgccaga atgctggtca gcatacgaaa gagcttaagg
     4741 caggccaatt ccactgtcag ggtcacttgg gtgtttagca ctaccgacag gtacgctagt
     4801 atgcgttctt cctaccagag gtctgtggcc gcgtggtcaa aagtgcggct ttcgtatttg
     4861 ctgctcgtgt ttactctcac aaacttgacc tgcacgccaa agagatgctt cttgtggaac
     4921 tcgacaacgc aacaacgcga cggatctacg tcacagcgtg catagtgaaa acggagttgc
     4981 tgacgacgaa agcgacattg ggatctgtca gttgtcattc gcgaaaaaca tccgtccccg
     5041 aggcggacac tgattgacgc ggttttgtag aaggttaggg gaataggtta gattgagtgg
     5101 cttaagaatg taaaatctgg gattatagtg tagtaatctc tgattaacgg tgacggtttt
     5161 aagacaggtg ttcgcaaaat caagcggggt catttcaaca gatattgctg atggtttagg
     5221 cgtacaatgc cctgaagaat aattaagaaa aaagcacccc tcgtcgccta gaattaccta
     5281 ccgcggtcca ccataccttc gattatcgcg cccactctcc cattagtcgg cagaggtggt
     5341 tgtgttgcga tagcccagta tgatattcta aggcgttacg ctgatgaata ttctacagag
     5401 ttgccatagg cgttgaacgc tacacggacg atacgaattt acgtatagag cgggtcatcg
     5461 aaaggttata ctctcgtagt taacatctag cccggcccta tcagtacagc agtgccttga
     5521 atgacatact catcattaaa ttttctctac agccaaacga ccaagtgcat ttccagggag
     5581 cgcgatggag attcattctc tcgccagcac tgtaataggc actaaaagag tgatgataat
     5641 catgagtgcc gcgctaaggt ggtgtcggaa caaagcggtc ttacggtcag tcgtattcct
     5701 tctcgagttc cgtccagttg agcgtgtcac tcccagtgta cctgcaagcc gagatggctg
     5761 tgcttggagt caatcgcatg tagcatggtc accagacacc ggggcaccag ttttcacgcc
     5821 taaagcataa acgacgagca gtcatgaaag tcttagtact ggacgtgccg tttcactgcg
     5881 aatatgactc cctcttctct caccaaccag tcacgaccgc ttcacgctaa ggtgctggcc
     5941 acgtgctaac cttcttctct caccaaccag tctggcgttg atgtggatcg actctatagc
     6001 catcttctct caccaaccag tccgaggccg ttcgtttccc gtcctaatac ctctacgtgc
     6061 aagcagtata aaaacaaaat acataaacat aactaaaatc tcatatcgtt gtaattgtca
     6121 tatactagtg gtaattaagt ccagttacac ttaaggttaa atttgctgac tcttttttac
     6181 ctttcatttc tgaaaatggt tcctaataag ggttcttctg atgtcttgtc cgaacattct
     6241 gagccacagt tcactaagct gatcgcccct caagccggac caaggaagta taagatcgtg
     6301 taccgaaact tactgacttt cggatattgg cacctttctg ctgtttatgg cttgtacctt
     6361 tgtttcactt gtgctaaatg ggcaacgatt ttgtttgcct tttttcttta cgtgatagct
     6421 gaaattggga ttactggtgg agctcacaga ctttgggcac atagaactta caaggctaaa
     6481 ctgcccctag aaattcttct tctcattatg aactccatag cttttcaaga tactgcattt
     6541 acttgggcca gggaccatag acttcaccac aaatactcag atactgatgc agatcctcac
     6601 aacgcaaccc ggggtttttt ttattcccac gttgggtggt tgttggtcaa aaagcatcca
     6661 gaggttaagg ctagaggaaa gtacttgtct ctcgatgact taaagaataa cccgctattg
     6721 aagttccaaa aaaaatacgc catactcgta attggaacct tatgtttttt gatgcctacg
     6781 ttcgtcccgg tatacttttg gggtgaaggc atctctactg catggaacat caatctttta
     6841 cgatatgtca tgaacttaaa tatgactttc ctagtcaact ctgctgccca tatatttggg
     6901 aacaagccgt atgataaatc aatagcttct gtgcaaaata tatcagtttc cctggctaca
     6961 tttggggagg gtttccataa ttatcatcac acatatccct gggattatcg tgcagcagaa
     7021 cttggaaaca atagattgaa tatgacaacc gcctttattg atttcttcgc ctggataggt
     7081 tgggcatatg atctaaagtc tgtgccacaa gaggctattg caaaacggtg cgcaaaaact
     7141 ggagatggta ctgatatgtg gggtaggaaa cgttgagctt aaacccaacc atacataata
     7201 aaaaggcgag ctacctcacc aacatgttct tggttcttga tggctgcttg aggtttttca
     7261 acacaacatg agtaaaatgg tcaataaata tggatattct catcttgctt gattcgggca
     7321 ggtaggatat cagagaattc taggtatatt ttgtagggaa aaaaaactag aatctcttca
     7381 ggatctggat cctaatacaa ggacatatta atattccatt ttataaactt tgcacaagca
     7441 agatctaatt ttatggaaga actgaaggct ctcgcgtatc atttgccaaa ataattacca
     7501 tcttttgtaa tcatcttgga aactagagat aatattgata atatccatag taataataat
     7561 aataagaaga acaaatatca tcataacaca ataatatagc cttttcatgt aaaatttcat
     7621 ttcaatgaat agccatgtta gcccattttc tttcaggaaa gtttatgctt gcttacatga
     7681 agaaacaaaa aaaattatga gaaacagaat ttactcacta ctgtgcaaat tatatgttca
     7741 actgagcaaa caagtcttca actacctctc ctcagagttg aatctgttcc atgcttccta
     7801 tagacacaaa agaacgataa ccactaagat aattcaaaga agataataaa agagaatgga
     7861 ccatgggaag aagaggggga ggtgaagctg gcgctgtcag gagctaactg agatactacc
     7921 atagatgact acccatccct ctaggcctta gataaccgga tacagtgact ttgataggtt
     7981 tgtggggtac agctatgact tgcatagctg cgtatggagg aaggaacttt tgcgtgttag
     8041 tatgttgacc cgtgtattac gcatgcgggt agatcatgtg ggtagagaca tccaggtcaa
     8101 gttctcgacc ttctcgtggg aggtgatcca gttcactata ggaccattcc attcgagcat
     8161 ggcactaagt acgccgtccc cattctggta atcatcatcc ctatcagggt ttggagtgag
     8221 tggtgacggt tattccccag gaatggactt cttattcaca gtcggtcaca ttgggctact
     8281 ccttgggtct tccgcttggc ccggtctgtt gggccgccat tgcgtgagtt tcggccctgt
     8341 gctgcgctgt gtcggcgatt ctcattgggg cctcacatct ggaaaccccg acctattttg
     8401 acggcaccat tggcggaagt tgctgggctt gtgcaccgtg aagtcctccg cgggcctccc
     8461 ccctacaaaa gacgataagc tccggcaagc agcattgatc aacgcaagga tcggcgatat
     8521 taacagagat acggctgatt actcttgttg gtgtggtatc gctaaactgc gccgcggagc
     8581 cttatggcaa agtcgtccgc gaaccattcc ggtagcgctt aaggtccata gcacattcat
     8641 cgcatcccgg cgtgcgttca atttgacgac cccttggcgc aaaggtgctg gccacgtgct
     8701 aaattaaagc ggctgcactg ctgtaaggtc cgttacggag agggcggcct ggggagagca
     8761 ctagcccatc gacctgtaca ggaacactct atattgttct cggacggaca gattactaga
     8821 gtgccgcttt cagcccccct gtcgtcgccg acgtctgtaa tatggcgttg ttgtgattcc
     8881 actctattga ggcattaact gatgcgtaag gagatctgga atgaactggc ctatgtcact
     8941 gaaactgtgc aaacacccaa tgtcgttagt gtaggttctg accgatacct gcttcgttga
     9001 gaactcacaa ttttacaact ggggacataa tccctacgcc catcatctac tcgcgtccct
     9061 gtggctccag ttcatgtaat gggagagtat cctccacaag atctagtgct atggtggtat
     9121 agtaagctcg tactgtggta gatgactccc tcttctctca ccaaccagtc aacgccccgt
     9181 accgatgctg aacaagtcga tgcaggccct tcttctctca ccaaccagtc caatcccgag
     9241 gcctgacccg acatatccca tcttctctca ccaaccagtc gtagctaact atgttcccgt
     9301 cctaatacct ctacgtgcaa gcagtataaa aacaaaatac ataaacataa ctaaaatctc
     9361 atatcgttgt aattgtcata tactagtggt aattaagtcc agttacactt aaggttaaat
     9421 ttgctgactc ttttttacct ttcatttctg aaaatggttg tgttgactag caaggagaca
     9481 aagcctagcg tcgctgaatt ttacgctgga aaaagtgttt ttattactgg gggtactggg
     9541 tttctcggta aggttttcat tgagaaactc ttgtattctt gtccagatat agaaaacatt
     9601 tacatgttga tcagggaaaa aaaaggttta tcagttagtg agaggatcaa gcagtttctt
     9661 gacgaccctc tgtttaccag gttgaaggat aagaggcctg ctgatcttga gaaaatcgtc
     9721 cttattcctg gcgacattac tgctcccgac ttgggtatta acagtgagaa cgaaaaaatg
     9781 cttatagaaa aggtgagtgt tatcatacac tcagcagcta ccgtcaagtt caatgagcca
     9841 ttgcctaccg cttggaagat caatgtcgag ggtacacgga tgatgttagc acttagcaga
     9901 cggatgaaga gaatagaggt tttcatccat atctctaccg cttacacaaa tacaaacagg
     9961 gaggtggttg atgaaatttt gtaccctgca cctgctgaca ttgaccaagt tcaccagtac
    10021 gtgaaagatg gtatctctga agaagatact gagaagatcc tcaatggaag gcccaataca
    10081 tatactttca ccaaggctct gaccgagcat ttagttgctg agaaccaagc ttatgtgcca
    10141 accattatcg ttaggccgag tgttgttgct gctataaagg acgagccact taaaggatgg
    10201 ctgggcaatt ggtttggcgc taccggattg acagttttta ccgcaaaggg actaaacagg
    10261 gtcatttatg gtcattcctc atacatagtt gatcttattc ctgtagatta cgttgctaat
    10321 ttagttatcg ccgcaggagc caagtcttca aaatctacag aattgaaagt gtataattgc
    10381 tgctcttcaa gctgcaaccc agtgaccatt gggacgttaa tgagtatgtt cgctgacgac
    10441 gctataaagc agaaaagtta tgcaatgccg ttaccgggtt ggtacatatt taccaagtat
    10501 aagtggcttg tgctactgtt gactttctta tttcaggtca ttcctgctta tgttacagat
    10561 ctctctaggc atctgattgg taaatcccca cgttatatca agctccaatc cctggttaat
    10621 cagactagat catctataga tttctttacc aaccacagct gggttatgaa ggccgaccgg
    10681 gtgagggagt tgtacgcatc actgtctccc gccgataaat acctattccc gtgtgatcct
    10741 accgatataa attggactca ttatatacag gattattgtt ggggtgtgag gcacttcttg
    10801 gaaaagaaat atcggtgagc ttctttgtgg ttattattta gcttctgtac actaaattta
    10861 tgatgcaaga agcgttgtac acaacatata gaagaagagt gcgaggtgaa gcaagtagga
    10921 gaaatgttag gaaagctcct atacaaaagg atggcatgtt gaagattagc atctttttaa
    10981 tcccaagttt aaatataaag catattttat gtaccacttt ctttatctgg ggtttgtaat
    11041 ccctttatat ctttatgcaa tctttacgtt agttaatatc tatctatcga tattctagta
    11101 tcttatacta tagatccaac tgaaccaaga aattatgaac cgtgtcttcc agaaattcta
    11161 ataatgatgg gagcaatata aatataagga tgtctttgac aataaaaggg cggtggaaga
    11221 gttatagtga aagcttctgg ccatcaaatg ggctctttgt cacatgattg aactacttca
    11281 ccacctaccg ttggtatcca tagtaaattg agattaaaaa aagttaaaac aaactattca
    11341 ttccatgtca ttttactttt caacttgcta tcttctaatt ataagtgggt tgtggagaga
    11401 gcactgttgg gtcagcaaag caaagtagat tatactctat aatattatga tgattagtca
    11461 actgctgctc acttacaacg atggaaatgg ggatgagggt aactttacgc tgtcaagcgg
    11521 ggtttagcca ttaacatcgt cgtcaaagtt cgatttctgg agtgtgaaaa attagggttt
    11581 cgtcgatagg agatgaaaag tcgaaatttc ggtgcgagaa atcaaaattg atggaagatc
    11641 ggcggcgaat ttgagaggat agtgagaaga agaagattaa gagatgattt gaattgggag
    11701 ggaaaatcga atcgccagtg tttgttttag ggtttttaat ctccgggaat tgaattttgg
    11761 gcgtcgtaaa actgtaaagt gtgtttatgt agtaatcttt atctgagatg atacagaaga
    11821 gattctatcc cagatacggt aaatttgttt tcccctaggt cggtggtcaa cattgcattt
    11881 ttaagtcaac ttttaatata ttttagttta atttctataa tttaaccttt ttggcattat
    11941 ctttaggaaa tttttttgac gtattttatt ttacttatta aaagtgaaaa atcttaaaca
    12001 tattaattaa tatcttgcat aaccaattga cattgtaatc atcacaatta aatttgatta
    12061 aaaatccact ttctgcaaac aaacaaagtt ttttttaata aagtctataa catatcgagt
    12121 ataatagtat caatatagat tttgatgata acaacaatag ccactcctga cggagtgttt
    12181 catgtccaag cgaaaagccg ctctacggaa tggatctacg ttactgcctg cataaggaga
    12241 tcggtgtagc caaggacgaa agcgacccta ggttctaacc gtcgactttg gcggaaaggt
    12301 ttcactcagg aagcagacac tgattgacac ggtttagcag aacgtttgag gactaggtca
    12361 aattgagtgg tttaatatcg gcatgtctgg ctttaaaatt cagtatagtg cgctgatcgg
    12421 agatgaatta aaaacacgag ttcccaaaac caggcgggct cgccacgtcg gctaatcctg
    12481 gtacattatg tgaacaatgt tctgaagaaa atttgtgaaa gaaggacggg tcatcgccta
    12541 ctattagcaa caacggtcgg ccacaccttc cattgtcgtg gccacgctcg gattacacgg
    12601 cagaggtgct tgtgttccga caggctagca tattatccta aggcgttacc ccaatcgttt
    12661 accgtcggat ttgctatagc ccctgaacgc tacatgtacg aaaccatgtt atgtatgcac
    12721 taggtcaaca ataggacata gccttgtagt taacacgtag cccggtcgta taagtacagt
    12781 agacccttcg ccggcatcct atttattaag ttatttctac agcaaaacga tcatatgcag
    12841 atccgcagtg ggcggtagac acacgtccac ccggctgctc tgtaacaggg actaaagagg
    12901 tgatgattat cgtgtgtgcc ccgttatggt cgtgttcgat cagagcgccc ttgcgagcag
    12961 tcgtatgctt tctcgaattc cgagcggtta agcgtgacag tcccagcgaa cccacaaaac
    13021 gtgatcgcag tccatgcgat catacgcaag aaggaaggtc cccatacacc gacgcaccag
    13081 tttacacgcc gtatgcataa acgagctgca caaacgagag tgtttgaact ggacctctag
    13141 ttcctctaca aagaacacct tgacctgttg cgaagttgcc ttgcctagat gcaatgtcgg
    13201 acgtatcact tttgcctcaa cgactgctgc tttcgctgta acccaagaca gacaacagta
    13261 accgcctttt gaaggcaagt cctccgcctg tgactaactg tgccaaatcg tcttccaaac
    13321 cccttatcca atttaactca ccaaattatt gcgatacaga ccctaatttc acatcatatg
    13381 acactaattg cctctgccaa aattctgtcc tcaatgactc cctcttctct caccaaccag
    13441 tcagttgggt tcacccgggt cggacctgag tcgaccaagc cttcttctct caccaaccag
    13501 tcgcgaccgc agaacttacg ctcgagggtc cgtcttctct caccaaccag tctccccggt
    13561 tatctctccc gtcctaatac ctctacgtgc aagcagtata aaaacaaaat acataaacat
    13621 aactaaaatc tcatatcgtt gtaattgtca tatactagtg gtaattaagt ccagttacac
    13681 ttaaggttaa atttgctgac tcttttttac ctttcatttc tgaaaatgaa tgaaatcgat
    13741 gaaaaaaatc aagctcccgt acaacaggag tgtctcaagg aaatgataca gaacggccac
    13801 gcaaggagga tgggcagtgt agaggacctc tacgtagcct taaacagaca aaacctgtat
    13861 agaaactttt gtacgtatgg ggaactttcc gactactgca cccgagatca actgacatta
    13921 gccctgaggg agatttgtct caaaaatcca acccttctgc atatagtctt gccaacccgt
    13981 tggccaaacc atgagaacta ttatcgttct tccgaatatt attcaagacc ccatcctgtc
    14041 catgattata taagcgtact ccaagagttg aagctttctg gggtagtgct taatgaacaa
    14101 ccggaatact ctgcagtgat gaagcaaatc cttgaagaat ttaagaattc aaaaggtagc
    14161 tatacggcaa aaatcttcaa gttgacaact actttgacga taccctactt cgggcctacc
    14221 ggtccttcat ggagattgat ctgtctcccc gaggaacata cagagaagtg gaggaagttc
    14281 atttttgtta gcaaccactg tatgtcagat ggcaggtcca gtatacattt tttccatgat
    14341 cttagagatg agcttaacaa tataaagacc cctccaaaga agttagacta tatctttaag
    14401 tatgaagagg attaccagtt attaaggaaa ctccctgagc ctatagagaa agttatagac
    14461 tttaggccac cgtatttatt catcccgaag agccttctta gcggattcat ctataaccac
    14521 ttacgattta gttccaaagg agtgtgtatg cgaatggatg acgttgagaa aacggatgac
    14581 gtcgtaacag aaataataaa tattagccct acagaatttc aagctatcaa ggccaatatt
    14641 aagagtaaca tccaagggaa atgtacgatc acgccctttt tgcatgtgtg ttggtttgta
    14701 tcccttcaca agtggggaaa atttttcaag ccccttaact tcgaatggct gactgacatt
    14761 tttataccag ctgattgcag gtcccaactc ccagacgatg atgaaatgcg acagatgtat
    14821 agatatggcg ctaatgtagg gtttatagac ttcactccct ggataagcga gtttgatatg
    14881 aacgacaaca aagaaaactt ctggccctta attgaacact accacgaggt tatttctgaa
    14941 gccttgagga ataagaagca tcttcatggc cttggcttta acattcaggg attcgttcaa
    15001 aagtatgtca atatagataa ggttatgtgc gaccgtgcta ttggaaaaag acgtggcggt
    15061 acgctcttga gcaatgtggg tttgtttaat caattggaag agccggacgc caaatattct
    15121 atatgcgact tagcctttgg tcaatttcag ggaagctggc accaagcctt cagtcttgga
    15181 gtatgtagca ctaacgtcaa aggaatgaac atagtggttg catcaactaa aaacgtggta
    15241 ggatctcagg agagcttaga ggaattatgt tccatttaca aagctctgct gttagggcct
    15301 taggcttacg aaaatatagt tgaaacagag cagagatcat ggtgttagcc ttctatggct
    15361 gaagaaaaat agttgaaaaa atgtgtgtgt ctagtgtttt agtgtgtctg tctgttgaaa
    15421 acagggaaaa aaatgtgttc ttttttccct gtaataagaa atatagtaat ggagtcttta
    15481 tgtgtaagta accagtaaaa tggtttgtgt aaacatgaat atctctgcat ctgttaattt
    15541 taacatataa gtttggatat gtgttgtggt ttcaactaat tcaagtactt ccttgataat
    15601 gttttcctgt ttctaggctg caaaactata gtaaatcttt taggttttgg taaaacctaa
    15661 ttccattggg tgtggttgga gagcatatag tttttgcgac gctctccgct gtcatgagac
    15721 cggatcctga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
    15781 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
    15841 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
    15901 cagtcggctt ctgacgttca gtgcagccgt catctgaaaa cgacatgtcg cacaagtcct
    15961 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
    16021 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
    16081 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
    16141 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
    16201 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
    16261 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
    16321 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
    16381 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
    16441 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
    16501 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
    16561 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
    16621 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
    16681 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
    16741 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
    16801 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acctctcaac
    16861 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
    16921 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
    16981 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
    17041 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
    17101 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
    17161 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
    17221 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
    17281 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
    17341 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
    17401 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
    17461 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
    17521 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
    17581 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
    17641 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
    17701 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
    17761 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
    17821 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
    17881 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
    17941 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
    18001 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
    18061 gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
    18121 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
    18181 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
    18241 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
    18301 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
    18361 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
    18421 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
    18481 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
    18541 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
    18601 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
    18661 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
    18721 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
    18781 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
    18841 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
    18901 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
    18961 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
    19021 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
    19081 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
    19141 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
//
