LOCUS       sequence-378065-        8014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent human androgen receptor (fused to EGFP) with expanded 
            polyglutamine region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino 
            GN, Marcelli M, Weigel NL, Mancini MA
  TITLE     Polyglutamine-expanded androgen receptors form aggregates that 
            sequester heat shock proteins, proteasome components and SRC-1, and 
            are suppressed by the HDJ-2 chaperone.
  JOURNAL   Hum Mol Genet. 1999 May . 8(5):731-41.
  PUBMED    10196362
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 1999 May . 8(5):731-41."
COMMENT     Sequence Label: pEGFP-C1-AR Q48
FEATURES             Location/Qualifiers
     source          1..8014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      613..622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(619..621,622..624,625..1335)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(619..621,622..624,625..1335)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(664..685)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(925..944)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1272..1293
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4808..4929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4845..4864)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4899..4918
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4936..5391)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5073..5094)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5285..5304
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5418..5522
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5524..5881
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5524..5715
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5547..5567)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5732..5867
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5916..6710
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5916..6710
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5970..5989)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6580..6599
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6898..6917)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6942..6989
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7318..7906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7807..7826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      661 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      721 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      781 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      841 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      901 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      961 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1021 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1081 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1141 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1201 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1261 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1321 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
     1381 ggtaccatgg aagtgcagtt agggctggga agggtctacc ctcggccgcc gtccaagacc
     1441 taccgaggag ctttccagaa tctgttccag agcgtgcgcg aagtgatcca gaacccgggc
     1501 cccaggcacc cagaggccgc gagcgcagca cctcccggcg ccagtttgct gctgcagcag
     1561 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
     1621 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag
     1681 cagcagcagc agcagcagca agagactagc cccaggcagc agcagcaggg tgaggatggt
     1741 tctccccaag cccatcgtag aggccccaca ggctacctgg tcctggatga ggaacagcaa
     1801 ccttcacagc cgcagtcggc cctggagtgc caccccgaga gagattgcgt cccagagcct
     1861 ggagccgccg tggccgccag caaggggctg ccgcagcagc tgccagcacc tccggacgag
     1921 gatgactcag ctgccccatc cacgttgtcc ctgctgggcc ccactttccc cggcttaagc
     1981 agctgctccg ctgaccttaa agacatcctg agcgaggcca gcaccatgca actccttcag
     2041 caacagcagc aggaagcagt atccgaaggc agcagcagcg ggagagcgag ggaggcctcg
     2101 ggggctccca cttcctccaa ggacaattac ttagggggca cttcgaccat ttctgacaac
     2161 gccaaggagt tgtgtaaggc agtgtcggtg tccatgggcc tgggtgtgga ggcgttggag
     2221 catctgagtc caggggaaca gcttcggggg gattgcatgt acgccccact tttgggagtt
     2281 ccacccgctg tgcgtcccac tccttgtgcc ccattggccg aatgcaaagg ttctctgcta
     2341 gacgacagcg caggcaagag cactgaagat actgctgagt attccccttt caagggaggt
     2401 tacaccaaag ggctagaagg cgagagccta ggctgctctg gcagcgctgc agcagggagc
     2461 tccgggacac ttgaactgcc gtctaccctg tctctctaca agtccggagc actggacgag
     2521 gcagctgcgt accagagtcg cgactactac aactttccac tggctctggc cggaccgccg
     2581 ccccctccgc cgcctcccca tccccacgct cgcatcaagc tggagaaccc gctggactac
     2641 ggcagcgcct gggcggctgc ggcggcgcag tgccgctatg gggacctggc gagcctgcat
     2701 ggcgcgggtg cagcgggacc cggttctggg tcaccctcag ccgccgcttc ctcatcctgg
     2761 cacactctct tcacagccga agaaggccag ttgtatggac cgtgtggtgg tggtgggggt
     2821 ggtggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg cggcgaggcg
     2881 ggagctgtag ccccctacgg ctacactcgg ccccctcagg ggctggcggg ccaggaaagc
     2941 gacttcaccg cacctgatgt gtggtaccct ggcggcatgg tgagcagagt gccctatccc
     3001 agtcccactt gtgtcaaaag cgaaatgggc ccctggatgg atagctactc cggaccttac
     3061 ggggacatgc gtttggagac tgccagggac catgttttgc ccattgacta ttactttcca
     3121 ccccagaaga cctgcctgat ctgtggagat gaagcttctg ggtgtcacta tggagctctc
     3181 acatgtggaa gctgcaaggt cttcttcaaa agagccgctg aagggaaaca gaagtacctg
     3241 tgcgccagca gaaatgattg cactattgat aaattccgaa ggaaaaattg tccatcttgt
     3301 cgtcttcgga aatgttatga agcagggatg actctgggag cccggaagct gaagaaactt
     3361 ggtaatctga aactacagga ggaaggagag gcttccagca ccaccagccc cactgaggag
     3421 acaacccaga agctgacagt gtcacacatt gaaggctatg aatgtcagcc catctttctg
     3481 aatgtcctgg aagccattga gccaggtgta gtgtgtgctg gacacgacaa caaccagccc
     3541 gactcctttg cagccttgct ctctagcctc aatgaactgg gagagagaca gcttgtacac
     3601 gtggtcaagt gggccaaggc cttgcctggc ttccgcaact tacacgtgga cgaccagatg
     3661 gctgtcattc agtactcctg gatggggctc atggtgtttg ccatgggctg gcgatccttc
     3721 accaatgtca actccaggat gctctacttc gcccctgatc tggttttcaa tgagtaccgc
     3781 atgcacaagt cccggatgta cagccagtgt gtccgaatga ggcacctctc tcaagagttt
     3841 ggatggctcc aaatcacccc ccaggaattc ctgtgcatga aagcactgct actcttcagc
     3901 attattccag tggatgggct gaaaaatcaa aaattctttg atgaacttcg aatgaactac
     3961 atcaaggaac tcgatcgtat cattgcatgc aaaagaaaaa atcccacatc ctgctcaaga
     4021 cgcttctacc agctcaccaa gctcctggac tccgtgcagc ctattgcgag agagctgcat
     4081 cagttcactt ttgacctgct aatcaagtca cacatggtga gcgtggactt tccggaaatg
     4141 atggcagaga tcatctctgt gcaagtgccc aagatccttt ctgggaaagt caagcccatc
     4201 tatttccaca cccagtgaag cattggaaac cctatttccc caccccagct catgccccct
     4261 ttcagatgtc ttctgcctgt tataactctg cactactcct ctgcagtgcc ttggggaatt
     4321 tcctctattg atgtacagtc tgtcatgaac atgttcctga attctatttg ctgggctttt
     4381 tttttctctt tctctccttt ctttttcttc ttccctccct atctaaccct cccatggcac
     4441 cttcagactt tgcttcccat tgtggctcct atctgtgttt tgaatggtgt tgtatgcctt
     4501 taaatctgtg atgatcctca tatggcccag tgtcaagttg tgcttgttta cagcactact
     4561 ctgtgccagc cacacaaacg tttacttatc ttatgccacg ggaagtttag agagctaaga
     4621 ttatctgggg aaatcaaaac aaaaacaagc aaacaaaaaa aaaagaaaaa cggaattcag
     4681 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
     4741 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4801 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4861 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4921 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     4981 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     5041 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     5101 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     5161 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     5221 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     5281 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     5341 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     5401 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5461 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5521 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5581 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5641 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5701 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5761 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5821 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5881 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5941 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6001 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6061 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     6121 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6181 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6241 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6301 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6361 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     6421 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     6481 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6541 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6601 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6661 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6721 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6781 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6841 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     6901 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     6961 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     7021 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     7081 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     7141 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     7201 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7261 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7321 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7381 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7441 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7501 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7561 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7621 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7681 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7741 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7801 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7861 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7921 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7981 atcccctgat tctgtggata accgtattac cgcc
//
