LOCUS       sequence-378067-       16423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of PUM1-rABE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16423)
  AUTHORS   Lin Y, Kwok S, Hein AE, Thai BQ, Alabi Y, Ostrowski MS, Wu K, Floor 
            SN
  TITLE     RNA molecular recording with an engineered RNA deaminase.
  JOURNAL   Nat Methods. 2023 Dec;20(12):1887-1899. doi: 
            10.1038/s41592-023-02046-z. Epub 2023 Oct 19.
  PUBMED    37857907
REFERENCE   2  (bases 1 to 16423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-02046-z"; journalName: "Nat Methods"; date: 
            "2023-12"; volume: "20"; issue: "12"; pages: "1887-1899"
COMMENT     Sequence Label: pSLIK-PUM1-rABE-Flag
FEATURES             Location/Qualifiers
     source          1..16423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        109..423
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    295..313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    331..349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     396..420
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4620..4643
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(4661..4685)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4735..5946
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5783..5802
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6071..6688,6689..6694,6695..6730,6731..6733,6734..6769,
                     6770..6772,6773..6808,6809..6817)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6868..7441
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7035..7052)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7262..7281
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7485..8084
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7485..7504)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7981..8001
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8550..9138
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8603..8623)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9021..9032)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9141..9157)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9142..9158)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9667..9847
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9873..9890)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9879..10103
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10149..10577
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10236..10255)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10446..10467
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10586..10606)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10591..10920
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10771..10906
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10833..10852
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10968..11015
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11034..11408
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11538..11659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11575..11594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11629..11648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11708..11724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11708..11724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11721..11743)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11732..11748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11756..11762,11763..11780,11781..11786))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11801..11822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11939..11956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12110..12698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12190..12209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12869..13660,13661..13729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13492..13511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13730..13834)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13909..13928)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        14100..14479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14481..14679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             14697..14877
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    14924..15049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    15542..15775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             15960..16004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    16302..16419
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tttattacag ggacagcaga gatccagttt ggttaattct agacagttat caacaagttt
       61 gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac tggatcccga gtttactccc
      121 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgatgtcg
      181 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      241 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttatcccta
      301 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      361 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
      421 gccactagtc caccatgagc gttgcatgtg tcttgaagag aaaagcagtg ctttggcagg
      481 actctttcag cccccacctg aaacatcacc ctcaagaacc agctaatccc aacatgcctg
      541 ttgttttgac atctggaaca gggtcgcaag cgcagccaca accagctgca aatcaggctc
      601 ttgcagctgg gactcactcc agccctgtcc caggatctat aggagttgca ggccgttccc
      661 aggacgacgc tatggtggac tacttctttc agaggcagca tggtgagcag cttgggggag
      721 gaggaagtgg aggaggcggc tataataata gcaaacatcg atggcctact ggggataaca
      781 ttcatgcaga acatcaggtg cgttccatgg atgaactgaa tcatgatttt caagcacttg
      841 ctctggaggg aagagcgatg ggagagcagc tcttgccagg taaaaagttt tgggaaacag
      901 atgaatccag caaagatgga ccaaaaggaa tattcctggg tgatcaatgg cgagacagtg
      961 cctggggaac atcagatcat tcagtttccc agccaatcat ggtgcagaga agacctggtc
     1021 agagtttcca tgtgaacagt gaggtcaatt ctgtactgtc cccacgatcg gagagtgggg
     1081 gactaggcgt tagcatggtg gagtatgtgt tgagctcatc cccgggcgat tcctgtctaa
     1141 gaaaaggagg atttggccca agggatgcag acagtgatga aaacgacaaa ggtgaaaaga
     1201 agaacaaggg tacgtttgat ggagataagc taggagattt gaaggaggag ggtgatgtga
     1261 tggacaagac caatggttta ccagtgcaga atgggattga tgcagacgtc aaagatttta
     1321 gccgtacccc tggtaattgc cagaactctg ctaatgaagt ggatcttctg ggtccaaacc
     1381 agaatggttc tgagggctta gcccagctga ccagcaccaa tggtgccaag cctgtggagg
     1441 atttctccaa catggagtcc cagagtgtcc ccttggaccc catggaacat gtgggcatgg
     1501 agcctcttca gtttgattat tcaggcacgc aggtacctgt ggactcagca gcagcaactg
     1561 tgggactttt tgactacaat tctcaacaac agctgttcca aagacctaat gcgcttgctg
     1621 tccagcagtt gacagctgct cagcagcagc agtatgcact ggcagctgct catcagccgc
     1681 acatcggttt agctcccgct gcgtttgtcc ccaatccata catcatcagc gctgctcccc
     1741 cagggacgga cccctacaca gctggattgg ctgcagcagc gacactaggc ccagctgtgg
     1801 tccctcacca gtattatgga gttactccct ggggagtcta ccctgccagt cttttccagc
     1861 agcaagctgc cgctgccgct gcagcaacta attcagctaa tcaacagacc accccacagg
     1921 ctcagcaagg acagcagcag gttctccgtg gaggagccag ccaacgtcct ttgaccccaa
     1981 accagaacca gcagggacag caaacggatc cccttgtggc agctgcagca gtgaattctg
     2041 cccttgcatt tggacaaggt ctggcagcag gcatgccagg ttatccggtg ttggctcctg
     2101 ctgcttacta tgaccaaact ggtgcccttg tagtgaatgc aggcgcgaga aatggtcttg
     2161 gagctcctgt tcgacttgta gctcctgccc cagtcatcat tagttcctca gctgcacaag
     2221 cagctgttgc agcagccgca gcttcagcaa atggagcagc tggtggtctt gctggaacaa
     2281 caaatggacc atttcgccct ttaggaacac agcagcctca gccccagccc cagcagcagc
     2341 ccaataacaa cctggcatcc agttctttct acggcaacaa ctctctgaac agcaattcac
     2401 agagcagctc cctcttctcc cagggctctg cccagcctgc caacacatcc ttgggattcg
     2461 gaagtagcag ttctctcggc gccaccctgg gatccgccct tggagggttt ggaacagcag
     2521 ttgcaaactc caacactggc agtggctccc gccgtgactc cctgactggc agcagtgacc
     2581 tttataagag gacatcgagc agcttgaccc ccattggaca cagtttttat aacggcctta
     2641 gcttttcctc ctctcctgga cccgtgggca tgcctctccc tagtcaggga ccaggacatt
     2701 cacagacacc acctccttcc ctctcttcac atggatcctc ttcaagctta aacctgggag
     2761 gactcacgaa cggcagtgga agatacatct ctgctgctcc aggcgctgaa gccaagtacc
     2821 gcagtgcaag cagcgcctcc agcctcttca gcccgagcag cactcttttc tcttcctctc
     2881 gtttgcgata tggaatgtct gatgtcatgc cttctggcag gagcaggctt ttggaagatt
     2941 ttcgaaacaa ccggtacccc aatttacaac tgcgggagat tgctggacat ataatggaat
     3001 tttcccaaga ccagcatggg tccagattca ttcagctgaa actggagcgt gccacaccag
     3061 ctgagcgcca gcttgtcttc aatgaaatcc tccaggctgc ctaccaactc atggtggatg
     3121 tgtttggtaa ttacgtcatt cagaagttct ttgaatttgg cagtcttgaa cagaagctgg
     3181 ctttggcaga acggattcga ggccacgtcc tgtcattggc actacagatg tatggctgcc
     3241 gtgttatcca gaaagctctt gagtttattc cttcagacca gcaggtaatt aatgagatgg
     3301 ttcgggaact agatggccat gtcttgaagt gtgtgaaaga tcagaatggc aatcacgtgg
     3361 ttcagaaatg cattgaatgt gtacagcccc agtctttgca atttatcatc gatgcgttta
     3421 agggacaggt atttgcctta tccacacatc cttatggctg ccgagtgatt cagagaatcc
     3481 tggagcactg tctccctgac cagacactcc ctattttaga ggagcttcac cagcacacag
     3541 agcagcttgt acaggatcaa tatggaaatt atgtaatcca acatgtactg gagcacggtc
     3601 gtcctgagga taaaagcaaa attgtagcag aaatccgagg caatgtactt gtattgagtc
     3661 agcacaaatt tgcaagcaat gttgtggaga agtgtgttac tcacgcctca cgtacggagc
     3721 gcgctgtgct catcgatgag gtgtgcacca tgaacgacgg tccccacagt gccttataca
     3781 ccatgatgaa ggaccagtat gccaactacg tggtccagaa gatgattgac gtggcggagc
     3841 caggccagcg gaagatcgtc atgcataaga tccggcccca catcgcaact cttcgtaagt
     3901 acacctatgg caagcacatt ctggccaagc tggagaagta ctacatgaag aacggtgttg
     3961 acttagggcc catctgtggc ccccctaatg gtatcatctc aggtgggtcc agtgggggct
     4021 ccagcgggtc agaaactcct gggacgagtg aatctgcgac ccccgagtca tccgggggta
     4081 gctccggtgg ttccatgtct gaggtggaat tctcgcacga atactggatg agacacgcat
     4141 tgactctcgc aaagagggct cgggatgaac gcgaggtgcc cgtgggggca gtactcgtgc
     4201 ttaacaatcg cgtaatcggc gaaggttgga atagggcgat cggactccac gaccccactg
     4261 cacatgcgga aatcatggcc cttcgacagg gagggcttgt gatgcagaat tatcgactta
     4321 tcgatgcgac gctgtacgtc acgtttgaac cttgcgtaat gtgcgcggga gctatgattc
     4381 actcccgcat tggacgagtt gtattcggtg tccgcaacgc caagacgggt gccgcaggtt
     4441 cactgatgga cgtgctgcat tacccaggca tgaaccaccg ggtagaaatc acagaaggca
     4501 tattggcgga cgaatgtgcg gcgctgttga gctacttttt tcgcatgccg aggcaggtat
     4561 ttaatgcgca aaagaaggct cagtcttcaa cagatagcgg tggaagcagt ggaggctcag
     4621 attacaagga tgacgatgac aagtgactcg agatatctag acccagcttt cttgtacaaa
     4681 gtggttgata acatctcgag ccataaagat ggttaattaa cccacccaag atctggcctc
     4741 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     4801 cagacgaagg gcgcagcgaa cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     4861 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     4921 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     4981 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     5041 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     5101 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     5161 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     5221 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     5281 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
     5341 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     5401 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     5461 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     5521 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     5581 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     5641 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     5701 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     5761 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     5821 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     5881 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     5941 ttagacgaag cttggtaccg agctcggatc tccaccccgt accggtcctg caggatttaa
     6001 atggcgcgcc gctagcgcta ccggactcag atctcgagct caagcttcga attctgcagt
     6061 cgaattcacc atgtctagac tggacaagag caaagtcata aacggagctc tggaattact
     6121 caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     6181 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     6241 cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga
     6301 ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc
     6361 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     6421 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     6481 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     6541 aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt
     6601 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     6661 ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt
     6721 agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc
     6781 tcttgacgat tttgaccttg acatgctccc cgggtaacta agtaaggatc cgcggccgca
     6841 ctagaggaat tccgcccctc tccccccccc ctctccctcc ccccccccta acgttactgg
     6901 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     6961 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     7021 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     7081 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     7141 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     7201 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     7261 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     7321 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     7381 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     7441 agcttaccgg tacgcgtgat agcttgccac aacccgtacc aaagatgacc gagtacaagc
     7501 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     7561 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     7621 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     7681 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     7741 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     7801 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     7861 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     7921 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     7981 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8041 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaagtaga tgccgaccga
     8101 acaagagctg atttcgagaa cgcctcagcc agcaactcgc gcgagcctag caaggcaaat
     8161 gcgagagaac ggccttacgc ttggtggcac agttctcgtc cacagttcgc taagctcgct
     8221 cggctgggtc gcgggagggc cggtcgcagt gattcaggcc cttctggatt gtgttggtcc
     8281 ccagggcacg attgtcatgc ccacgcactc gggtgatctg actgatcccg cagattggag
     8341 atcgccgccc gtgcctgccg attgggtgca gatctctaga tctgtacaag taaagcggcc
     8401 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     8461 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgtttagtc
     8521 cctcccaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     8581 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     8641 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     8701 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     8761 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8821 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8881 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8941 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     9001 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     9061 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     9121 tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga
     9181 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     9241 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     9301 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     9361 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9421 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     9481 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     9541 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     9601 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     9661 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9841 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     9901 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     9961 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
    10021 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
    10081 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
    10141 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    10201 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    10261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    10321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    10381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    10441 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    10501 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    10561 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
    10621 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
    10681 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
    10741 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
    10801 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
    10861 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    10921 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
    10981 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
    11041 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
    11101 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
    11161 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
    11221 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
    11281 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
    11341 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
    11401 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
    11461 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
    11521 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    11581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    11641 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
    11701 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    11761 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    11821 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    11881 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    11941 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    12001 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    12061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    12121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    12181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    12241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    12301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    12361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    12421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    12481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    12541 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    12661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    12721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    12781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    12841 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    12901 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    12961 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    13021 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    13081 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    13141 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    13201 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    13261 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    13321 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    13381 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    13441 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    13501 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    13561 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    13621 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    13681 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    13741 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    13801 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    13861 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
    13921 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
    13981 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
    14041 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
    14101 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
    14161 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
    14221 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
    14281 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
    14341 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
    14401 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
    14461 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
    14521 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
    14581 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
    14641 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt
    14701 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    14761 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    14821 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
    14881 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
    14941 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
    15001 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
    15061 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
    15121 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
    15181 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
    15241 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
    15301 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
    15361 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
    15421 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
    15481 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
    15541 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
    15601 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
    15661 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
    15721 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
    15781 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
    15841 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
    15901 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
    15961 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    16021 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
    16081 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
    16141 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
    16201 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc
    16261 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
    16321 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
    16381 aaagaattac aaaaacaaat tacaaaaatt caaaattttc ggg
//
