LOCUS       sequence-378083-       16488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of PUM2-rABE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16488)
  AUTHORS   Lin Y, Kwok S, Hein AE, Thai BQ, Alabi Y, Ostrowski MS, Wu K, Floor 
            SN
  TITLE     RNA molecular recording with an engineered RNA deaminase.
  JOURNAL   Nat Methods. 2023 Dec;20(12):1887-1899. doi: 
            10.1038/s41592-023-02046-z. Epub 2023 Oct 19.
  PUBMED    37857907
REFERENCE   2  (bases 1 to 16488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-02046-z"; journalName: "Nat Methods"; date: 
            "2023-12"; volume: "20"; issue: "12"; pages: "1887-1899"
COMMENT     Sequence Label: pSLIK-PUM2-rABE-Flag
FEATURES             Location/Qualifiers
     source          1..16488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             42..86
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    384..501
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    558..582
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        614..928
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    622..640
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    658..676
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    693..711
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    729..747
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    765..783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    800..818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    836..854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     901..925
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4753..4776
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(4794..4818)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4868..6079
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5916..5935
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6204..6821,6822..6827,6828..6863,6864..6866,6867..6902,
                     6903..6905,6906..6941,6942..6950)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7003..7576
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7170..7187)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7397..7416
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7635..8654
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             7635..8654
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    9120..9708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9173..9193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9591..9602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9711..9727)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9712..9728)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10237..10417
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10443..10460)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10449..10673
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10719..11147
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10806..10825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11016..11037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11156..11176)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11161..11490
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11341..11476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11403..11422
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11538..11585
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11604..11978
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12108..12229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12145..12164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12199..12218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12278..12294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12278..12294)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12291..12313)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12302..12318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12326..12332,12333..12350,12351..12356))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12371..12392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12509..12526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12680..13268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12760..12779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13439..14230,14231..14299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14062..14081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14300..14404)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14479..14498)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        14670..15049
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15051..15249
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             15267..15447
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    15494..15619
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    16112..16345
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
       61 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
      121 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
      181 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
      241 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
      301 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
      361 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      421 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      481 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
      541 attctagaca gttatcaaca agtttgtaca aaaaagcagg ctttaaagga accaattcag
      601 tcgactggat cccgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
      661 ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc
      721 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
      781 agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct
      841 atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag
      901 agctcgttta gtgaaccgtc agatcgccac tagtccacca tgaatcatga ttttcaagct
      961 cttgcattag aatctcgggg aatgggagag cttttgccta ccaaaaagtt ttgggaacct
     1021 gatgattcaa caaaagatgg acaaaaaggc atatttcttg gggatgatga atggagagag
     1081 actgcatggg gagcttctca ccattcaatg tcccagccta ttatggtaca gagaagatct
     1141 ggacagggtt ttcatggaaa cagtgaagta aatgcaatac tgtctccgcg atcagaaagt
     1201 ggaggccttg gtgtgagcat ggtagaatat gtattaagtt cttctcctgc tgataaattg
     1261 gattctcgat ttaggaaggg aaattttggc actagagatg ctgaaacaga tggacctgag
     1321 aaaggagatc aaaaaggcaa ggcttctcca tttgaggagg accaaaacag agatcttaaa
     1381 caaggagatg atgatgattc taaaataaat ggcagaggtt tgccaaatgg aatggatgcc
     1441 gattgcaaag attttaatcg tactcctgga agtcgtcaag cctctccaac tgaagtagtt
     1501 gagcgcttgg gccccaatac taatccctca gaaggactgg ggcctcttcc taatcctaca
     1561 gctaataaac cacttgttga agaattttca aatcctgaaa ctcagaatct ggatgccatg
     1621 gaacaagttg gtctggaatc cttacagttt gactatcctg gtaatcaggt accaatggac
     1681 tcttcaggag ctactgtagg cctttttgac tacaattccc agcagcagct ctttcagagg
     1741 actaatgcac taacagttca acagttaact gcagctcaac agcagcaata tgcattagca
     1801 gcagctcagc agccacatat agctggtgta ttctcagcag gccttgctcc agctgcattt
     1861 gtgccaaatc catacattat tagtgctgct cctccaggga ccgatccgta tactgcagca
     1921 ggattggctg cagcagctac attagcaggt ccagcagtgg ttccacctca gtattacggc
     1981 gttccatggg gggtgtatcc agccaactta tttcagcagc aagctgcagc tgcggcaaat
     2041 aacacagcca gtcagcaagc agcatcacaa gctcagcctg gacagcaaca ggttctccgt
     2101 gctggagcag gtcagcgtcc tcttactccc aatcagggtc agcaagggca gcaagcagaa
     2161 tcacttgcgg cagctgcagc agcaaatcca acattggctt ttggtcaggg tcttgctact
     2221 ggcatgccag gctatcaagt actagctcca actgcctatt atgatcagac tggtgcctta
     2281 gtggttggcc ctggagcaag gactggcctt ggagctccag ttcggttaat ggctccaaca
     2341 cctgttttaa ttagttcagc agcagcacaa gctgcagcag cagcagcagc tggaggaact
     2401 gcaagtagcc ttacaggcag cacaaatggt ctgtttcggc caattggcac tcagccacca
     2461 cagcagcagc aacagcagcc aagcactaat ctgcaatcta attcatttta tggaagcagt
     2521 tctttgacta atagctccca gagtagttct ttattttctc atggacctgg tcaacctgga
     2581 agtacatctc ttggctttgg aagtggtaac tctttgggtg ctgctatagg ctcagccctc
     2641 agtggatttg gttcatcagt tggcagttct gcaagtagta gtgccacaag gagagagtct
     2701 ctatctacta gctctgactt gtacaaaaga tctagtagca gcctagcacc catagggcaa
     2761 ccattttaca atagtctggg attttcctcc tctccaagtc caataggcat gcctctgcca
     2821 agccaaactc caggacattc acttacgcca ccgccatcac tttcatcaca tggatcctca
     2881 tccagtttgc atttaggagg actgacaaat ggtagtggtc gatatatctc tgcagcacct
     2941 ggagcagaag caaaatatcg aagtgcttca agcacttcca gtctatttag ctccagcagc
     3001 cagctctttc ctccttcccg gcttcggtat aataggtctg atattatgcc ttctggccgc
     3061 agtagattat tggaagattt cagaaacaac cgcttcccaa accttcagct tagagacttg
     3121 attggacata tagttgagtt ttctcaagac cagcatggtt ctagattcat acagcaaaaa
     3181 ctagagagag ctactccagc tgagcgacag atggtattta atgaaattct gcaagcagcc
     3241 tatcaattaa tgactgatgt ttttggcaac tatgttatac agaagttttt tgagtttggg
     3301 agtctggatc aaaaattagc cctggctact cgtattcgtg gtcatgttct acccttagcc
     3361 ttgcagatgt atggctgccg cgttattcag aaagcattag aatctatttc ttctgaccag
     3421 cagagtgaaa tggtaaagga gctggatggt catgtgctca aatgtgtgaa agatcagaat
     3481 ggaaaccatg ttgtacaaaa atgtatcgaa tgtgttcagc cacagtcact acagttcatc
     3541 attgatgctt tcaagggaca agtatttgtg ctttcaactc atccttatgg ctgcagagta
     3601 attcagcgca tcctagagca ttgcactgca gaacagacct tacctatctt agaagaactc
     3661 caccaacata cagagcagtt ggtacaggat cagtatggca attatgttat tcagcatgta
     3721 ctggaacacg gtcgacctga agacaagagc aaaattgttt ccgaaatcag gggaaaggtt
     3781 ttagccctga gtcaacacaa atttgccagc aatgtagtag aaaagtgtgt tactcatgcc
     3841 tcccgtgctg agagagcttt actgattgac gaggtttgct gccagaatga tggtcctcac
     3901 agtgccttat acaccatgat gaaggaccag tatgccaatt acgtggttca aaagatgatt
     3961 gatatggctg aacctgctca gagaaagata atcatgcaca agattcgacc tcacattact
     4021 actttgcgca aatacacata cgggaagcat atactggcca agttggaaaa gtattatttg
     4081 aagaatagcc cggacctagg acctattgga ggaccaccaa atggaatgct gtcaggtggg
     4141 tccagtgggg gctccagcgg gtcagaaact cctgggacga gtgaatctgc gacccccgag
     4201 tcatccgggg gtagctccgg tggttccatg tctgaggtgg aattctcgca cgaatactgg
     4261 atgagacacg cattgactct cgcaaagagg gctcgggatg aacgcgaggt gcccgtgggg
     4321 gcagtactcg tgcttaacaa tcgcgtaatc ggcgaaggtt ggaatagggc gatcggactc
     4381 cacgacccca ctgcacatgc ggaaatcatg gcccttcgac agggagggct tgtgatgcag
     4441 aattatcgac ttatcgatgc gacgctgtac gtcacgtttg aaccttgcgt aatgtgcgcg
     4501 ggagctatga ttcactcccg cattggacga gttgtattcg gtgtccgcaa cgccaagacg
     4561 ggtgccgcag gttcactgat ggacgtgctg cattacccag gcatgaacca ccgggtagaa
     4621 atcacagaag gcatattggc ggacgaatgt gcggcgctgt tgagctactt ttttcgcatg
     4681 ccgaggcagg tatttaatgc gcaaaagaag gctcagtctt caacagatag cggtggaagc
     4741 agtggaggct cagattacaa ggatgacgat gacaagtgac tcgagatatc tagacccagc
     4801 tttcttgtac aaagtggttg ataacatctc gagccataaa gatggttaat taacccaccc
     4861 aagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
     4921 agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc
     4981 aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac
     5041 attttaggac gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca
     5101 ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa
     5161 cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga
     5221 tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg
     5281 ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag
     5341 agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg
     5401 gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg
     5461 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg
     5521 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg
     5581 ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg
     5641 cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg
     5701 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt
     5761 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct
     5821 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct
     5881 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg
     5941 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt
     6001 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt
     6061 tttggctttt ttgttagacg aagcttggta ccgagctcgg atctccaccc cgtaccggtc
     6121 ctgcaggatt taaatggcgc gccgctagcg ctaccggact cagatctcga gctcaagctt
     6181 cgaattctgc agtcgaattc accatgtcta gactggacaa gagcaaagtc ataaacggag
     6241 ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     6301 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     6361 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     6421 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     6481 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     6541 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     6601 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     6661 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     6721 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     6781 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     6841 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     6901 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg
     6961 atccgcggcc gcactagagg aattccgccc ctctcccccc cccccctctc cctccccccc
     7021 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     7081 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     7141 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     7201 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     7261 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     7321 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     7381 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     7441 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     7501 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     7561 aaaaacacga tgataagctt accggtacgc gtgatagctt gccacaaccc gtaccaaaga
     7621 tggatagatc cggaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     7681 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     7741 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     7801 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     7861 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     7921 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     7981 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     8041 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     8101 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     8161 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     8221 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     8281 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     8341 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     8401 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     8461 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     8521 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     8581 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     8641 gggcaaagga atagagtaga tgccgaccga acaagagctg atttcgagaa cgcctcagcc
     8701 agcaactcgc gcgagcctag caaggcaaat gcgagagaac ggccttacgc ttggtggcac
     8761 agttctcgtc cacagttcgc taagctcgct cggctgggtc gcgggagggc cggtcgcagt
     8821 gattcaggcc cttctggatt gtgttggtcc ccagggcacg attgtcatgc ccacgcactc
     8881 gggtgatctg actgatcccg cagattggag atcgccgccc gtgcctgccg attgggtgca
     8941 gatctctaga tctgtacaag taaagcggcc gcgactctag atcataatca gccataccac
     9001 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     9061 taaaatgaat gcaattgttg ttgtttagtc cctcccaatt cgatatcaag cttatcgata
     9121 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     9181 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     9241 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     9301 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     9361 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     9421 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     9481 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     9541 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     9601 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     9661 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     9721 gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     9781 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     9841 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     9901 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     9961 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
    10021 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag
    10081 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat
    10141 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt
    10201 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc
    10261 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    10321 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    10381 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
    10441 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
    10501 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
    10561 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
    10621 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
    10681 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
    10741 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    10801 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    10861 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    10921 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    10981 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    11041 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    11101 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
    11161 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    11221 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    11281 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
    11341 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    11401 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
    11461 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
    11521 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
    11581 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
    11641 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
    11701 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
    11761 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
    11821 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
    11881 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
    11941 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
    12001 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
    12061 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
    12121 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    12181 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
    12241 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    12301 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    12361 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    12421 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    12481 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    12541 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    12601 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    12661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    12721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    12781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    12841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    12901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    12961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    13021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    13081 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    13141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    13201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    13261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    13321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    13381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    13441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    13501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    13561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    13621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    13681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    13741 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    13801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    13861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    13921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    13981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    14041 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    14101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    14161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    14221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    14281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    14341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    14401 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
    14461 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    14521 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    14581 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    14641 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
    14701 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
    14761 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
    14821 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
    14881 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
    14941 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
    15001 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
    15061 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
    15121 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
    15181 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
    15241 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
    15301 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    15361 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    15421 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
    15481 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
    15541 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
    15601 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
    15661 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
    15721 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
    15781 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
    15841 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
    15901 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
    15961 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
    16021 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
    16081 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
    16141 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
    16201 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    16261 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    16321 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    16381 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    16441 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagct
//
