LOCUS       sequence-378102-       11279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the split-AND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 11279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP689_L2_1IF2_FRT-OCS_Rluc_C1_FlpF2
FEATURES             Location/Qualifiers
     source          1..11279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1587..1620
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5680..5735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6190..6210
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6248..6422
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    6492..6513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6528..6533,6534..6551,6552..6558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6566..6582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6571..6593
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6590..6606
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6590..6606
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6602..6925
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    6619..6675
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6676..6693)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6676..6692)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6685..6707)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6901..6920)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    7057..7081
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7169..7879
                     /label=oriV
                     /note="incP origin of replication"
     promoter        7900..8004
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8005..8799
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8970..9558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9459..9478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9894..11042
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11188..11212
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta taggaacttc
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct
     5401 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
     5461 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
     5521 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
     5581 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
     5641 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
     5701 caacaaacaa cattacaatt actatttaca attacaatga gcacaaccaa aaaagccagt
     5761 gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag
     5821 ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt
     5881 gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat
     5941 agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag
     6001 agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggcagc
     6061 ctctccggag ggtctggctc cggatcaagt ggtggcagcg gtacttcgag catcagatac
     6121 cccgcctgga acggcatcat cagccaggag gtgctggact acctgagcag ctacatcaac
     6181 aggcggatcc ccaagaaaaa gcggaaggtg tgagcttctc tagctagagt cgatcgacaa
     6241 gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta
     6301 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     6361 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga
     6421 tcgctttact gagaccgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6481 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6541 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6601 tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg atccccgggt
     6661 accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6721 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6781 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     6841 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     6901 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     6961 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgacggtctc
     7021 agggacacga agtgatccgt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     7081 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     7141 atccgttcgt ccatttgtat gtgccagccg cctttgcgac gctcaccggg ctggttgccc
     7201 tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc
     7261 cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac ttggccctca
     7321 ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc gcggcgttga
     7381 cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg
     7441 gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc ctggggataa
     7501 gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg gcgcgatcct
     7561 tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca tttgaggggc
     7621 tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc ggccaccgct
     7681 aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt aaccagggct
     7741 gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct cgaaccctcc
     7801 cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc cgcgaacggc
     7861 ctcaccccaa aaatggcagc gctggccaat tcccgagtgc gcggaacccc tatttgttta
     7921 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     7981 caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga attgaaaaaa
     8041 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
     8101 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
     8161 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
     8221 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
     8281 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
     8341 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
     8401 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
     8461 gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg cgagctgtat
     8521 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
     8581 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
     8641 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag ggaggatatc
     8701 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     8761 aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca agtttactca
     8821 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8881 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8941 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9001 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9061 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9121 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9181 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9241 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9301 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9361 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9421 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9481 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9541 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggcagatcct
     9601 agatgtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc cgcatcaagt
     9661 gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg ctggtattcg
     9721 tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc tacgggaccg
     9781 acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg tcaaatcagg
     9841 aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg tgaatgaatc
     9901 ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt tccgccgagg
     9961 atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg
    10021 tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg caactggctc
    10081 cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctt gaacaggagg
    10141 cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg accaagaagc
    10201 gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc
    10261 tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat attgcgccgt
    10321 ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg ttcaccacgc
    10381 gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac gtcaacaagg
    10441 acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc
    10501 agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc ttcacgttct
    10561 acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg aaggccgagg
    10621 aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc gttgggcacc
    10681 tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc
    10741 gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga
    10801 aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg ttcgactatt
    10861 tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat
    10921 cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc
    10981 gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat tgcaaacgct
    11041 agggccttgt ggggtcagtt ccggctgggg gttcagcagc ccctgctcgg atctgttgga
    11101 ccggacagta gtcatggttg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    11161 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
    11221 gcttagacaa cttaataaca cattgcggac gtttttaatg tactggggtt gaacactct
//
