LOCUS       sequence-378104-        9485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  On state for the second generation of NOT gates, which target the 
            Rluc promoter to repress expression via its B3RT sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ
FEATURES             Location/Qualifiers
     source          1..9485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    890..945
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          977..1165
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2193..2367
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2755..4407
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2821..2839)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4427..4627
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    4698..4719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4734..4739,4740..4757,4758..4764)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4772..4788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4777..4799
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4796..4812
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4796..4812
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4808..5131
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    4825..4881
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4882..4899)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4882..4898)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4891..4913)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5107..5126)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    5263..5287
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5375..6085
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6106..6210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6211..7005
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7176..7764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7665..7684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8100..9248
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9394..9418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gggttgctta agaataagta attcttaagc aacctcgaca
       61 aaatttagaa cgaacttaat tatgatctca aatacattga tacatatctc atctagatct
      121 aggttatcat tatgtaagaa agttttgacg aatatggcac gacaaaatgg ctagactcga
      181 tgtaattggt atctcaactc aacattatac ttataccaaa cattagttag acaaaattta
      241 aacaactatt ttttatgtat gcaagagtca gcatatgtat aattgattca gaatcgtttt
      301 gacgagttcg gatgtagtag tagccattat ttaatgtaca tactaatcgt gaatagtgaa
      361 tatgatgaaa cattgtatct tattgtataa atatccataa acacatcatg aaagacactt
      421 tctttcacgg tctgaattaa ttatgataca attctaatag aaaacgaatt aaattacgtt
      481 gaattgtatg aaatctaatt gaacaagcca accacgacga cgactaacgt tgcctggatt
      541 gactcggttt aagttaacca ctaaaaaaac ggagctgtca tgtaacacgc ggatcgagca
      601 ggtcacagtc atgaagccat caaagcaaaa gaactaatcc aagggctgag atgattaatt
      661 agtttaaaaa ttagttaaca cgagggaaaa ggctgtctga cagccaggtc acgttatctt
      721 tacctgtggt cgaaatgatt cgtgtctgtc gattttaatt atttttttga aaggccgaaa
      781 ataaagttgt aagagataaa cccgcctata taaattcata tattttcctc tccgctttga
      841 aggttgctta agaataagta attcttaagc aacctactgt atttttacaa caattaccaa
      901 caacaacaaa caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt
      961 gtatgatcct gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca
     1021 ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat
     1081 agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat
     1141 atgaccaaaa tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc
     1201 aagatgtaaa cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca
     1261 cgctgagaat gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca
     1321 tgttgtgcct cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg
     1381 aaagtctggt aaatctggaa atggttctta cagacttttg gatcattata agtacctcac
     1441 agcttggttc gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg
     1501 tgcttgcttg gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca
     1561 tgcagagagt gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga
     1621 tattgctttg atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt
     1681 ggaaactatg cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata
     1741 tctcgaacct ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga
     1801 aataccatta gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc
     1861 ttaccttagg gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt
     1921 cagtaatgct atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa
     1981 aggattgcat ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt
     2041 tgttgaaaga gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc
     2101 aacaagctcc cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc
     2161 ttctctagct agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc
     2221 ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac
     2281 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     2341 ccaaaatcca gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg
     2401 aatccccacc cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt
     2461 atttaaaaaa aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg
     2521 taatttaccg agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat
     2581 accaaactta ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt
     2641 cgattttcgt ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca
     2701 aacaagtaag taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag
     2761 gacgccaaaa acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct
     2821 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     2881 acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg
     2941 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     3001 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
     3061 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag
     3121 cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa
     3181 ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag
     3241 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta
     3301 ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact
     3361 gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc
     3421 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
     3481 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
     3541 gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt
     3601 caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac
     3661 aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa
     3721 gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc
     3781 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     3841 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     3901 ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta
     3961 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     4021 atagcttact gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt
     4081 aaatacaaag gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc
     4141 aacatcttcg acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc
     4201 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     4261 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     4321 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     4381 aagaagggcg gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat
     4441 caatttatgt attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct
     4501 gatataatca gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt
     4561 tgcttggact ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt
     4621 tcaagatcgc tactatgaga ccgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4681 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     4741 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     4801 aacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc
     4861 ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4921 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     4981 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     5041 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     5101 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5161 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5221 ggtctcaggg acacgaagtg atccgtttaa actatcagtg tttgacagga tatattggcg
     5281 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     5341 aggtttatcc gttcgtccat ttgtatgtgc cagccgcctt tgcgacgctc accgggctgg
     5401 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt
     5461 cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg
     5521 ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg
     5581 cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc
     5641 aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg
     5701 ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc
     5761 gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg
     5821 aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc
     5881 accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc
     5941 agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa
     6001 ccctcccggc ccgctaacgc gggcctccca tccccccagg ggctgcgccc ctcggccgcg
     6061 aacggcctca ccccaaaaat ggcagcgctg gccaattccc gagtgcgcgg aacccctatt
     6121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6181 atgcttcaat aatattgaaa aaggaagagt atggctaaaa tgagaatatc accggaattg
     6241 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
     6301 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
     6361 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
     6421 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
     6481 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
     6541 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
     6601 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
     6661 ctggccgatg tggattgcga aaactgggaa gaggacactc catttaaaga tccgcgcgag
     6721 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     6781 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     6841 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gctcagggag
     6901 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     6961 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagctgtc agaccaagtt
     7021 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7141 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7201 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7261 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7321 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7381 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7441 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7501 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7561 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7621 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7681 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7741 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggca
     7801 gatcctagat gtggcgcaac gatgccggcg acaagcagga gcgcaccgac ttcttccgca
     7861 tcaagtgttt tggctctcag gccgaggccc acggcaagta tttgggcaag gggtcgctgg
     7921 tattcgtgca gggcaagatt cggaatacca agtacgagaa ggacggccag acggtctacg
     7981 ggaccgactt cattgccgat aaggtggatt atctggacac caaggcacca ggcgggtcaa
     8041 atcaggaata agggcacatt gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa
     8101 tgaatcggac gtttgaccgg aaggcataca ggcaagaact gatcgacgcg gggttttccg
     8161 ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc
     8221 agtccgtcgg ctcgatggtc cagcaagcta cggccaagat cgagcgcgac agcgtgcaac
     8281 tggctccccc tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt cgtcttgaac
     8341 aggaggcggc aggtttggcg aagtcgatga ccatcgacac gcgaggaact atgacgacca
     8401 agaagcgaaa aaccgccggc gaggacctgg caaaacaggt cagcgaggcc aagcaggccg
     8461 cgttgctgaa acacacgaag cagcagatca aggaaatgca gctttccttg ttcgatattg
     8521 cgccgtggcc ggacacgatg cgagcgatgc caaacgacac ggcccgctct gccctgttca
     8581 ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca
     8641 acaaggacgt gaagatcacc tacaccggcg tcgagctgcg ggccgacgat gacgaactgg
     8701 tgtggcagca ggtgttggag tacgcgaagc gcacccctat cggcgagccg atcaccttca
     8761 cgttctacga gctttgccag gacctgggct ggtcgatcaa tggccggtat tacacgaagg
     8821 ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc gaccgcgttg
     8881 ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa
     8941 cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact
     9001 acacgaaatt catatgggag aagtaccgca agctgtcgcc gacggcccga cggatgttcg
     9061 actatttcag ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt
     9121 gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag
     9181 agttgcgagg cagcggcctg gtggaacacg cctgggtcaa tgatgacctg gtgcattgca
     9241 aacgctaggg ccttgtgggg tcagttccgg ctgggggttc agcagcccct gctcggatct
     9301 gttggaccgg acagtagtca tggttgatgg gctgcctgta tcgagtggtg attttgtgcc
     9361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     9421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact ggggttgaac
     9481 actct
//
