LOCUS       sequence-378108-       11962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the OR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11962)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 11962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP761_L2pV1_OR-OCS_s35S-Cre
FEATURES             Location/Qualifiers
     source          1..11962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(879..912)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      913..1620
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1621..1654
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(1655..1688)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          1755..1943
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2971..3145
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3533..5185
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3599..3617)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5205..5405
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        5507..5852
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5795..5815
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5949..5969
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6399..6587
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     misc_feature    7740..7764
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7852..8562
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8583..8687
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8688..9482
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9653..10241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10142..10161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10577..11725
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11871..11895
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcat aacttcgtat agcatacatt
      901 atacgaagtt atctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct
      961 ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc
     1021 cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata
     1081 ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa
     1141 caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa
     1201 caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct
     1261 aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta
     1321 cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc
     1381 gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg
     1441 gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta
     1501 tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg
     1561 acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag
     1621 gaagttccta ttctctagaa agtataggaa cttcataact tcgtatagca tacattatac
     1681 gaagttatca acaaacaaca ttacaattac tatttacaat tacaatgact tcaaaggtgt
     1741 atgatcctga gcaggtaagt ttctgcttct acctttgata tatatataat aattatcatt
     1801 aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag
     1861 caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat
     1921 gaccaaaatt tgttgatgtg cagagaaaaa gaatgataac tggtccacaa tggtgggcaa
     1981 gatgtaaaca gatgaacgtg cttgattctt tcattaatta ctacgattca gaaaagcacg
     2041 ctgagaatgc agttatcttt cttcacggaa acgctgcatc ttcatatttg tggagacatg
     2101 ttgtgcctca cattgaacca gtggctaggt gtattatccc agatcttatc ggaatgggaa
     2161 agtctggtaa atctggaaat ggttcttaca gacttttgga tcattataag tacctcacag
     2221 cttggttcga acttctcaac ttacctaaga agattatttt cgttggacac gattggggtg
     2281 cttgcttggc attccattac tcatacgaac accaagataa gatcaaagct atagttcatg
     2341 cagagagtgt tgtggatgtg atagaatctt gggatgagtg gccagatatt gaagaggata
     2401 ttgctttgat caagtctgaa gagggagaaa agatggttct cgaaaataac tttttcgtgg
     2461 aaactatgct tccttcaaag attatgagaa aattggaacc agaagagttt gctgcatatc
     2521 tcgaaccttt caaggaaaaa ggagaggtta gaaggccaac actcagttgg cctagggaaa
     2581 taccattagt taagggaggt aaacctgatg ttgtgcagat cgtgagaaat tataacgctt
     2641 accttagggc atcagatgat ttgcctaaga tgttcatcga gtcagatcca ggatttttca
     2701 gtaatgctat agttgaaggt gcaaagaaat ttccaaacac cgagttcgtt aaggtgaaag
     2761 gattgcattt ctcacaagaa gatgctcctg atgagatggg aaagtatatt aagtcttttg
     2821 ttgaaagagt gctcaaaaac gagcagggtt ctcgatctga tgcttctgaa gtaacgtcaa
     2881 caagctccca gtctgaggta tgcacagtgg aaacgccagg ttgtgttggt tcttaagctt
     2941 ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc
     3001 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     3061 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     3121 aaaatccagt actaaaatcc agatcgctgc aagaattcaa gcttggagcc aacactcgaa
     3181 tccccacccg ttggaccaaa cccggctcat taagcgtcgg ttcagattta tttcctttat
     3241 ttaaaaaaaa ggaaagggta aaaaatagaa aattggaaac agttaaagcc caaaattgta
     3301 atttaccgag aattgtaaat ttacctgaaa accctacgct atagtttcga ctataaatac
     3361 caaacttagg acctcacttc agaatcccct cgtcgctgcg tctctctccc gcaaccttcg
     3421 attttcgttt attcgcatcc atcggagaga gaaaacaatc aataagcgac ctactacaaa
     3481 caagtaagta agagaaaaac caaaagaaga agagaaacaa caagaagaag taatggagga
     3541 cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg
     3601 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     3661 agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt
     3721 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     3781 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     3841 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc
     3901 taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt
     3961 accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     4021 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc
     4081 agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg
     4141 gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag
     4201 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     4261 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     4321 cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca
     4381 aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa
     4441 atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt
     4501 cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac
     4561 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     4621 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     4681 cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa
     4741 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     4801 agcttactgg gacgaggacg aacacttctt catagttgac cgcttgaagt ctttaattaa
     4861 atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa
     4921 catcttcgac gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac ttcccgccgc
     4981 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     5041 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     5101 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     5161 gaagggcgga aagtccaaat tgtaagcttg atcccccgtc gacagctagc tatatcatca
     5221 atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga
     5281 tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg
     5341 cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc
     5401 aagatcgcta ctagaattcg agctcggagg tcaacatggt ggagcacgac actctggtct
     5461 actccaaaaa tgtcaaagat acagtctcag aagatcaaag ggctattgag acttttcaac
     5521 aaaggataat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     5581 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     5641 ctatcattca agatctctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     5701 gcatcgtgga aaaagaagag gttccaacca cgtctacaaa gcaagtggat tgatgtgaca
     5761 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     5821 tataaggaag ttcatttcat ttggagagga cacgctcgag tataagagct catttttaca
     5881 acaattacca acaacaacaa acaacaaaca acattacaat tacatttaca attatcgata
     5941 caatggtgcc caagaagaag aggaaagtct ccaacctgct gactgtgcac caaaacctgc
     6001 ctgccctccc tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca
     6061 gggacaggca ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct
     6121 gggctgcctg gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga
     6181 gggactacct cctgtacctg caagccagag gcctggctgt gaaaaccatc caacagcacc
     6241 tgggccagct caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg
     6301 ctgtgtccct ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca
     6361 agcaggccct ggcctttgaa cgcactgact ttgaccaggt aagtttctgc ttctaccttt
     6421 gatatatata taataattat cattaattag tagtaatata atatttcaaa tatttttttc
     6481 aaaataaaag aatgtagtat atagcaattg cttttctgta gtttataagt gtgtatattt
     6541 taatttataa cttttctaat atatgaccaa aatttgttga tgtgcaggtc agatccctga
     6601 tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca
     6661 acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg
     6721 atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg
     6781 tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg
     6841 gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg
     6901 ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc
     6961 accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc
     7021 actctgccag agtgggtgct gccagggaca tggccagggc tggtgtgtcc atccctgaaa
     7081 tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg
     7141 actctgagac tggggccatg gtgaggctgc tcgaggatgg ggactgagct tgctctcaag
     7201 atcaaaggct taaaaagctg gggttttatg aatgggatca aagtttcttt ttttctttta
     7261 tatttgcttc tccatttgtt tgtttcattt ccctttttgt tttcgtttct atgatgcact
     7321 tgtgtgtgac aaactctctg ggtttttact tacgtctgcg tttcaaaaaa aaaaaccgct
     7381 ttcgttttgc gttttagtcc cattgttttg tagctctgag tgatcgaatt gatgcctctt
     7441 tattcctttt gttccctata atttctttca aaactcagaa gaaaaacctt gaaactcttt
     7501 gcaatgttaa tataagtatt gtataagatt tttattgatt tggttattag tcttactttt
     7561 gctacctcca tcttcacttg gaactgatat tctgaatagt taaagcgtta catgtgttcc
     7621 attcacaaat gaacttaaac tagcacaaag tcagatattt taagatcgca ccatttcgct
     7681 ttacgaggat gcacatgtga ccgagggaca cgaagtgatc cgtttaaact atcagtgttt
     7741 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     7801 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgccag ccgcctttgc
     7861 gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa
     7921 cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc gttgtggata
     7981 cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc
     8041 cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg
     8101 gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat
     8161 gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac
     8221 tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc
     8281 gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt
     8341 ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat
     8401 aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg
     8461 tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc
     8521 tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agcgctggcc aattcccgag
     8581 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8641 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg gctaaaatga
     8701 gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag
     8761 gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa
     8821 tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc
     8881 tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct
     8941 ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg
     9001 aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca
     9061 tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt
     9121 acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagag gacactccat
     9181 ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg
     9241 tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg
     9301 gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg
     9361 tccggtcgct cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac
     9421 tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt
     9481 agctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9541 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9601 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9661 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9721 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9781 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9841 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9901 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9961 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10021 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10081 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10141 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10201 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10261 ttttacggtt cctggcagat cctagatgtg gcgcaacgat gccggcgaca agcaggagcg
    10321 caccgacttc ttccgcatca agtgttttgg ctctcaggcc gaggcccacg gcaagtattt
    10381 gggcaagggg tcgctggtat tcgtgcaggg caagattcgg aataccaagt acgagaagga
    10441 cggccagacg gtctacggga ccgacttcat tgccgataag gtggattatc tggacaccaa
    10501 ggcaccaggc gggtcaaatc aggaataagg gcacattgcc ccggcgtgag tcggggcaat
    10561 cccgcaagga gggtgaatga atcggacgtt tgaccggaag gcatacaggc aagaactgat
    10621 cgacgcgggg ttttccgccg aggatgccga aaccatcgca agccgcaccg tcatgcgtgc
    10681 gccccgcgaa accttccagt ccgtcggctc gatggtccag caagctacgg ccaagatcga
    10741 gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg
    10801 ttcgcgtcgt cttgaacagg aggcggcagg tttggcgaag tcgatgacca tcgacacgcg
    10861 aggaactatg acgaccaaga agcgaaaaac cgccggcgag gacctggcaa aacaggtcag
    10921 cgaggccaag caggccgcgt tgctgaaaca cacgaagcag cagatcaagg aaatgcagct
    10981 ttccttgttc gatattgcgc cgtggccgga cacgatgcga gcgatgccaa acgacacggc
    11041 ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa
    11101 ggtcattttc cacgtcaaca aggacgtgaa gatcacctac accggcgtcg agctgcgggc
    11161 cgacgatgac gaactggtgt ggcagcaggt gttggagtac gcgaagcgca cccctatcgg
    11221 cgagccgatc accttcacgt tctacgagct ttgccaggac ctgggctggt cgatcaatgg
    11281 ccggtattac acgaaggccg aggaatgcct gtcgcgccta caggcgacgg cgatgggctt
    11341 cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct
    11401 ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct
    11461 gtttgctggc gaccactaca cgaaattcat atgggagaag taccgcaagc tgtcgccgac
    11521 ggcccgacgg atgttcgact atttcagctc gcaccgggag ccgtacccgc tcaagctgga
    11581 aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc gcgagcaggt
    11641 cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct gggtcaatga
    11701 tgacctggtg cattgcaaac gctagggcct tgtggggtca gttccggctg ggggttcagc
    11761 agcccctgct cggatctgtt ggaccggaca gtagtcatgg ttgatgggct gcctgtatcg
    11821 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
    11881 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
    11941 atgtactggg gttgaacact ct
//
