LOCUS       sequence-378113-        4250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor for C-terminal tagging of endogenous hSLBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4250)
  TITLE     S-phase specific probe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hSLBP_mNG_FS_Donor
FEATURES             Location/Qualifiers
     source          1..4250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             619..1326
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     CDS             1339..1404
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(1414..1419,1420..1443,1444..1479,1480..1503)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     primer_bind     complement(2260..2277)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2431..3019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2511..2530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3190..3981,3982..4050))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3813..3832
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4051..4155)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4223..4241
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatggt accgtctagg cttttacaat tctttctgtg cttataaact atgtacatgg
      241 ccaggcacag tgactagtga ctccttcctg taatcccagc acaatgggat gtgtgcacat
      301 ctaaacgtca gtgtcttgta aatggtacat gtggtttctc tttgcagtgt tattattaaa
      361 atctgtctca gttgcacgtg aagtgtacac cttgctgtgc attacatcac atgatttacc
      421 acataaggag tggggtgtag gaaggggccc ctgttttcag ggtgagatga atgtcaaccg
      481 agccatgctt ctgttttcca ggatgtgtac tctggcacac ccaccaaggt gagacacatg
      541 gacagtcaag tggaggatga gtttgatttg gaagcttgtt taactgaacc cttgagagac
      601 ttctcagcca tgagcgccat ggtgagcaag ggcgaggagg ataacatggc ctctctccca
      661 gcgacacatg agttacacat ctttggctcc atcaacggtg tggactttga catggtgggt
      721 cagggcaccg gcaatccaaa tgatggttat gaggagttaa acctgaagtc caccaagggt
      781 gacctccagt tctccccctg gattctggtc cctcatatcg ggtatggctt ccatcagtac
      841 ctgccctacc ctgacgggat gtcgcctttc caggccgcta tggtagatgg ctccggatac
      901 caagtccatc gcacaatgca gtttgaagat ggtgcctccc ttactgttaa ctaccgctac
      961 acctacgagg gaagccacat caaaggagag gcccaggtga aggggactgg tttccctgct
     1021 gacggtcctg tgatgaccaa ctcgctgacc gctgcggact ggtgcaggtc gaagaagact
     1081 taccccaacg acaaaaccat catcagtacc tttaagtgga gttacaccac tggaaatggc
     1141 aagcgctacc ggagcactgc gcggaccacc tacacctttg ccaagccaat ggcggctaac
     1201 tatctgaaga accagccgat gtacgtgttc cgtaagacgg agctcaagca ctccaagacc
     1261 gagctcaact tcaaggagtg gcaaaaggcc tttaccgatg tgatgggcat ggacgagctg
     1321 tacaagggat ccgccggcga ctacaaggac cacgacggcg attataagga tcacgacatc
     1381 gactacaaag acgacgatga caagggcgcc agcagcgcct ggtcccaccc tcagtttgag
     1441 aagggcggag gctctggcgg cggaagcgga ggatctgctt ggagccaccc ccagttcgaa
     1501 aagtaactgc cccctggcgg ccaggaagag aaacagctcc tccccgacta ggtggaaggc
     1561 tggccaggca ccaagcatgt gtgtgcactt gtacctggtg gtttctctgt tagcagtcca
     1621 ttagctcatg ctgaattatt tttgccttac tttcttaaga aacattaatt ttatgtatag
     1681 tgagtatatt ttgcatgttt taaattgtaa atggagctaa gtccaagaaa gtacttgaag
     1741 ctctcttcca gcgagcttaa ttgcgtaatc cctgttgtcc tccagggtaa gctgacacgt
     1801 ctacataact ggttttccac aggcatcttc agttattgct tgtcaggtgg actgttttgg
     1861 atttaaccat gtaatccatg ggaccaattg agagtcagct acttttatag gcatcaaagt
     1921 attctcagac acctttaata tctttatgga aacttaattt ttggcctttt atcaatatgt
     1981 cataacagca ttctgaagtc agacattgtt aaattgagct attaaactaa tgagttttat
     2041 gtaagttata tggtcttaat ttggtacttg taaatagcac tagttagact ctttagaata
     2101 ctccaagagt tagggcagca gagtggagcg atttagaaag aacattttaa aacaatcagt
     2161 taatttacca tgtaaaattg ctgtaaatgg aattctgcat taatgaatcg gccaacgcgc
     2221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2341 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2941 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3001 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3061 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4201 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
