LOCUS       sequence-378116-        8861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination 
            with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate
            SLBP endogenous tagging by marker free coselection using ouabain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8861)
  TITLE     S-phase specific probe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA
FEATURES             Location/Qualifiers
     source          1..8861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        270..345
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        507..755
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        776..851
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        865..1150
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        1152..1429
                     /label=chicken beta-actin promoter
     intron          1430..1657
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1669..1678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1681..1701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1726..4263,4264..4266,4267..4728,4729..4731,4732..4899,
                     4900..4902,4903..5826)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5827..5874
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5902..5919)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5908..6115
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6124..6264
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6124..6253
                     /label=AAV2 ITR
     rep_origin      6339..6794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6426..6445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6636..6657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6811..6830)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6930..6952
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6990..7008)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7076..7180
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7181..7249,7250..8041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7399..7418)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8212..8800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8701..8720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg tgagctaact gccccctggg ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      421 acaaatggct ctagagctgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg
      481 gatccggtac caaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc
      541 atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga
      601 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
      661 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
      721 tggctttata tatcttgtgg aaaggacgaa acaccgagtt ctgtaattca gcatagtttt
      781 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
      841 cgagtcggtg ctttttttaa gctccgttac ataacttacg gtaaatggcc cgcctggctg
      901 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg
      961 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1021 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc
     1081 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1141 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1201 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1261 gggggggggg gggcgcgcgc crggsggggs ggggsggggs grggggsggg gsggggcgag
     1321 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     1381 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     1441 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     1501 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     1561 ttagctgagc aagaggtaag ggtttaaggg atggttggtt ggtggggtat taatgtttaa
     1621 ttacctggag cacctgcctg aaatcacttt ttttcaggtt ggaccggtgc caccatggcc
     1681 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
     1741 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1801 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1861 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     1921 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     1981 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     2041 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     2101 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     2161 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     2221 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     2281 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     2341 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     2401 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     2461 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     2521 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     2581 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     2641 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     2701 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2761 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2821 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2881 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     2941 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     3001 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     3061 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     3121 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     3181 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     3241 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     3301 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     3361 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     3421 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     3481 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     3541 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     3601 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3661 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3721 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3781 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3841 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3901 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     3961 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     4021 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     4081 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     4141 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     4201 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     4261 ctggccgacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     4321 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     4381 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     4441 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     4501 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     4561 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     4621 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4681 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctgc gctggaaagc
     4741 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4801 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4861 aagaccgaga ttaccctggc caacggcgag atccggaagg cgcctctgat cgagacaaac
     4921 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     4981 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     5041 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     5101 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     5161 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     5221 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     5281 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     5341 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     5401 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     5461 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     5521 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     5581 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     5641 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     5701 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     5761 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5821 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagtaagaa
     5881 ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5941 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6001 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6061 tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct ggggagcggc
     6121 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     6181 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     6241 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     6301 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     6361 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     6421 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6481 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6541 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6601 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6661 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     6721 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6781 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6841 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6901 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     6961 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7021 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7081 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7141 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7201 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7261 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7321 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7381 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7441 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7501 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7561 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7621 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7681 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7741 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7801 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7861 gtttattgct gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact
     7921 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7981 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8041 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8101 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8161 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8221 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8281 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8341 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8401 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8461 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8521 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8581 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8641 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8701 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8761 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8821 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
