LOCUS       sequence-378119-        8547 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lamp1 tagged with mGFP, expressed under the UAS promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Moller K, Brambach M, Villani A, Gallo E, Gilmour D, Peri F
  TITLE     A role for the centrosome in regulating the rate of neuronal 
            efferocytosis by microglia in vivo.
  JOURNAL   Elife. 2022 Nov 18;11:e82094. doi: 10.7554/eLife.82094.
  PUBMED    36398880
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-11-18"; volume: "11:e82094. doi"; pages: " 
            10.7554/eLife.82094"
COMMENT     Sequence Label: pDEST_6xUAS:Lamp1-mGFP
FEATURES             Location/Qualifiers
     source          1..8547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    144..278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     294..310
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    318..338
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    401..535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    537..561
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(578..1291,1292..1294,1295..1297))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     972..991
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1231..1252
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1294..1303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(1367..2533,2534..2617))
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     protein_bind    complement(2647..2671)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2751..2845
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    2770..2864
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(2880..2896)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2907..2927)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2947..2963)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(3336..3498,3499..3510)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     3859..3880
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4043..4147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4148..4216,4217..5008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4366..4385)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5179..5767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5668..5687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5921..5938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6055..6076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6091..6096,6097..6114,6115..6121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6129..6145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6134..6156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6153..6169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6188..6208
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6190..6208
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     6245..6261
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(6295..6306,6307..6494)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     CDS             7717..8430
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGANFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYYDLPSKLGHKLN"
ORIGIN
        1 attataagct gcaataaaca agagatctga tctagaggat cataatcagc cataccacat
       61 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      121 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
      181 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
      241 tgtccaaact catcaatgta tcttatcatg tctggatcat catcgatggt accgtaaaac
      301 gacggccagt gaattatcaa ctatgtataa taaagttgga aaaaacctcc cacacctccc
      361 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
      421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      481 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccccca
      541 ctttgtacaa gaaagctggg tcggcgcgcc caccctttta cttgtacagc tcgtccatgc
      601 cgagagtgat cccggcggcg gtcacgaact ccttaaggac catgtgatcg cgcttctcgt
      661 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
      721 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
      781 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
      841 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
      901 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
      961 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     1021 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     1081 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     1141 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     1201 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     1261 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgatggat cccgggcccg
     1321 cggtaccgtc gaccgcgccg gtggagcctg tggagccggt ggagccgata gtctggtagc
     1381 ctgcgtgact cctcttcctg ccgacgaggt aggcgatgag gacgatgagg accagccccg
     1441 ccagggcacc acccacagcg atggggatca gcatgctgtt ctcgtccagc agacactcct
     1501 ccacagagcc aaactggcca ccttccacct tgaaagcctg gacccacact ttgaatatat
     1561 tgactgaaaa cgccttcgtg acacggacgt gctcctccgc gttgcacttg taggaattgc
     1621 cgactgtggc ctgcagcgct cgcagggagc cgttggcagc tttaaaggcg gggtctctgg
     1681 cgtcaggaag aattgtattc aactggattc cttgtaggaa aaaccggcta gaacttgcat
     1741 tcatcccgaa ctggaagagc aggacggtgg tgccctcgct gtgcagctcc agagtcacca
     1801 ggtgggcgcc gcagctcccg ctggccgagg tcttgttggg gttgatgttg agaagccttg
     1861 tcaccgtcgt gttgtccttc ctctcatagg tgaggttcag ctgcagcccc atgctggcca
     1921 gcaggcaggt cccgttggtg ccgctcacgt tgtacttgtc cacagagggg ctcttgggca
     1981 cgggtgaggg cgaggggctg ggtggcgcag ggggcgctgt ggttggggaa ggcctgtctt
     2041 gttcacagcg tgtctctccc ctgctgaagc tgctgttgga aaggtacgcc tggatggtgg
     2101 catcatggag cgttacggtc acgttgttca tgtggacctg ggtgccacta acacatctgt
     2161 attttttatc tatatctgcc ctgatgtcag ttatagattc cacagtcttg atttctttgg
     2221 agctcgcatt ggggaaaagg tgtgtgtctg acaagttata aacaaaactc atgagctgga
     2281 cgctgtaacg tgttgcattt ctcgtgaaat tgagagtgag tgtatgtcct cttccaaaag
     2341 caatcacgag actggggtca gaagtgttct cttttccaca ggagctgcgg ttgagcacca
     2401 ctgtggcatc tgatggcagg tcaaaggtca tgttcttagg gccactcttg gtgtcgtagt
     2461 tcactgagaa ggcagcagag aagttggcca ttatgcacgc ggtcccgttg ccatttttca
     2521 ccataaacat tgctgctgac gcacaatgca tgaggccgag cagcagcaac agcagtagca
     2581 gcagcagggg tcgccgggcg ctgccggggg ccgccatgtc cctgaaggtg aagggggcgg
     2641 ccgcggagcc tgcttttttg tacaaacttg cggtggcggc gaattcgtgg aggagctcaa
     2701 agtgaggctg agacgcgatg ggatccatta tataccctct agagtctccg ctcggaggac
     2761 agtactccgc tcggaggaca gtactccgct cggaggacag tactccgctc ggaggacagt
     2821 actccgctcg gaggacagta ctccgctcgg aggacagtac tccgacctgc agcccggggg
     2881 atccactagt tctagagcgg ccgccgcaac ttttctatac aaagttgata gcttggcgta
     2941 atcatggtca tagctgtttc ctgctcgagg gcccatctgg cctgtgtttc agacaccagg
     3001 gagtctctgc tcacgtttcc tgctatttgc agcctctcta tcaagactaa tacacctctt
     3061 cccgcatcgg ctgcctgtga gaggcttttc agcactgcag gattgctttt cagccccaaa
     3121 agagctaggc ttgacactaa caattttgag aatcagcttc tactgaagtt aaatctgagg
     3181 ttttacaact ttgagtagcg tgtactggca ttagattgtc tgtcttatag tttgataatt
     3241 aaatacaaac agttctaaag caggataaaa ccttgtatgc atttcattta atgttttttg
     3301 agattaaaag cttaaacaag aatctctagt tttctttctt gcttttactt ttacttcctt
     3361 aatactcaag tacaatttta atggagtact tttttacttt tactcaagta agattctagc
     3421 cagatacttt tacttttaat tgagtaaaat tttccctaag tacttgtact ttcacttgag
     3481 taaaattttt gagtactttt tacacctctg tcaagaactc ctggacaaac ctctgacctg
     3541 tgtggaacag agtggatatg ggtgtctgaa cagatattca cgtcttttgc agatcagagg
     3601 gcatttctgg tgttgtgcaa tgacctgggt ccaacaggta ggcgtactcg tatccaagag
     3661 cattgttgga caggaggtag tctccattcc tataaagagg aaggaatggc accatgtagt
     3721 agccgtcatt gtggccaatt ggtgcattgg tacgtgggta gatggaccgg ggaggctgat
     3781 gagttcttag ccagcgtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3841 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3901 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3961 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     4021 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4081 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4141 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4201 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4261 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4321 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4381 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4441 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4501 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4561 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4621 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4681 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4741 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4801 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4861 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4921 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4981 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5041 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5101 tctcatgacc aaaatgcctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5161 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5221 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5281 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5341 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5401 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5461 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5521 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5581 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5641 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5701 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5761 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5821 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     5881 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     5941 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6001 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6061 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     6181 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg
     6241 ccgctctaga actagtggat ctgctgggct tgctgaaggt agggggtcaa gaaccagagg
     6301 tgtaaagtac ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt
     6361 ttactttact tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt
     6421 tagaaaaaaa agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt
     6481 tttggagatc acttcattct attttccctt gctattacca aaccaattga attgcgctga
     6541 tgcccagttt aatttaaatg ttatttattc tgcctatgaa aatcgttttc acattatatg
     6601 aaattggtca gacatgttca ttggtccttt ggaagtgacg tcatgtcaca tctattacca
     6661 caatgcacag caccttgacc tggaaattag ggaaattata acagtcaatc agtggaagaa
     6721 aatggaggaa gtatgtgatt catcagcagc tgcgagcagc acagtccaaa atcagccaca
     6781 ggatcaagag cacccgtggc cgtatcttcg cagatctaaa gcttaaatca gttgtgttaa
     6841 ataagagaca ttcaaaataa atgtaaatga gctctccaaa tcagcagact taacattctt
     6901 taaaatgatt gattcaatag tgataaaaat caggcatagc cagttgtaac tttagataaa
     6961 ttacagaaaa tgtcaaatac agagaaccga ttctttttta tgatacatcc aagcacacat
     7021 ttaacacaat ccaggcaaac cccgaatttc acagtcacaa gcactgtttg tacaagagct
     7081 ttgcctaagg acacacagtc tctataagtc caggtcgttg gtttcactct tattttaaac
     7141 atgtgacatt tttcctgcca tcctgtctta ggctgctgtt tgcttcattc catgtcacat
     7201 taaattcctc agtagcacct tttacacaca cagccaatct tttccagaaa attcaattgc
     7261 tttgaagaga taatgtgtga acaaatccat ttagaaaagg aaaattaaga atttgtaaaa
     7321 tcatctgtaa attgttggca ttcttctgta tatgaacatc acatcattta caggtaaagg
     7381 tctggtcatt aattatatga caatttactg gtattatttt gtgaaagggg ctattttcaa
     7441 tgcgttcatc catccttttc atccctcaaa tctctcattc acgtccccct ccccatctgc
     7501 acactttatc tcattttcca ccctgctgga atctgagcac ttgtgcagtt atcagggctc
     7561 ctgtatttag gaggctctgg gtgtccatgt aggggacgaa cagaaacact gcagaccttt
     7621 atagaagaac aattgataag agtcctcata cataaagact ccattagtaa gccagtgacc
     7681 caggagccca gaccaacagc aaagcagaca gtgaccatgg tgtctaaggg cgaagagctg
     7741 attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
     7801 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     7861 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
     7921 ggcagcagaa ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc
     7981 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     8041 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggcg
     8101 aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc
     8161 gagatgctgt accccgctga cggcggcctg gaaggcagaa gcgacatggc cctgaagctc
     8221 gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct
     8281 aagaacctca agatgcccgg tgtctactat gtggaccaca gactggaaag aatcaaggag
     8341 gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata ctacgacctc
     8401 cctagcaaac tggggcacaa acttaattga tacgtagatc cagacatgat aagatacatt
     8461 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     8521 tgtgatgcta ttgctttatt tgtaacc
//
