LOCUS       sequence-378125-       12654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the relative expression strength of the TCTP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP722_pSP15
FEATURES             Location/Qualifiers
     source          1..12654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    340..395
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          427..615
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    1643..1817
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        2830..3175
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3118..3138
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    3194..3249
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     complement(3317..3335)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3335..3523
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    5127..5301
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6181..6236
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6238..7263
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    7867..7888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7903..7908,7909..7926,7927..7933)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7941..7957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7946..7968
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7965..7981
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7965..7981
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7977..8300
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7994..8050
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8051..8068)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8051..8067)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8060..8082)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8276..8295)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8432..8456
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8544..9254
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9275..9379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9380..10174
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10345..10933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10834..10853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11269..12417
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12563..12587
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gccaacactc gaatccccac ccgttggacc aaacccggct
       61 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
      121 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
      181 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
      241 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
      301 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
      361 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
      421 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
      481 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
      541 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
      601 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
      661 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
      721 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
      781 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
      841 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
      901 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
      961 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     1021 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     1081 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     1141 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     1201 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     1261 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     1321 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     1381 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     1441 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     1501 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     1561 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     1621 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     1681 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     1741 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     1801 gtactaaaat ccagatcgct gcaagaattc aagcttggag gaattccaat cccacaaaaa
     1861 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
     1921 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
     1981 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
     2041 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
     2101 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
     2161 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
     2221 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
     2281 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
     2341 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
     2401 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
     2461 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
     2521 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
     2581 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
     2641 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
     2701 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
     2761 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
     2821 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     2881 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     2941 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     3001 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
     3061 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     3121 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgcta
     3181 ctgtattttt acaacaatta ccaacaacaa caaacaacaa acaacattac aattactatt
     3241 tacaattaca atggaggacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
     3301 ggaagatgga accgctggag agcaactgca taaggtaagt ttctgcttct acctttgata
     3361 tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa
     3421 taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat
     3481 ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggctatga agagatacgc
     3541 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
     3601 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
     3661 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
     3721 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
     3781 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
     3841 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
     3901 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
     3961 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
     4021 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
     4081 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
     4141 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     4201 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagagg agctgtttct
     4261 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
     4321 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     4381 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
     4441 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     4501 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     4561 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
     4621 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     4681 ggatggatgg ctacattctg gagacatagc ttactgggac gaggacgaac acttcttcat
     4741 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     4801 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtctacccga
     4861 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     4921 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     4981 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     5041 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt gagcttctct
     5101 agctagagtc gatcgacaag ctcgagtttc tccataataa tgtgtgagta gttcccagat
     5161 aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag
     5221 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     5281 tccagtacta aaatccagat cgctactaga attcgagctc ggaggaggtc attcatatgc
     5341 ttgagaagag agtcgggata gtccaaaata aaacaaaggt aagattacct ggtcaaaagt
     5401 gaaaacatca gttaaaaggt ggtataaagt aaaatatcgg taataaaagg tggcccaaag
     5461 tgaaatttac tcttttctac tattataaaa attgaggatg tttttgtcgg tactttgata
     5521 cgtcattttt gtatgaattg gtttttaagt ttattcgctt ttggaaatgc atatctgtat
     5581 ttgagtcggg ttttaagttc gtttgctttt gtaaatacag agggatttgt ataagaaata
     5641 tctttaaaaa aacccatatg ctaatttgac ataatttttg agaaaaatat atattcaggc
     5701 gaattctcac aatgaacaat aataagatta aaatagcttt cccccgttgc agcgcatggg
     5761 tattttttct agtaaaaata aaagataaac ttagactcaa aacatttaca aaaacaaccc
     5821 ctaaagttcc taaagcccaa agtgctatcc acgatccata gcaagcccag cccaacccaa
     5881 cccaacccaa cccaccccag tccagccaac tggacaatag tctccacacc cccccactat
     5941 caccgtgagt tgtccgcacg caccgcacgt ctcgcagcca aaaaaaaaaa aagaaagaaa
     6001 aaaaagaaaa agaaaaaaca gcaggtgggt ccgggtcgtg ggggccggaa acgcgaggag
     6061 gatcgcgagc cagcgacgag gccggccctc cctccgcttc caaagaaacg ccccccatcg
     6121 ccactatata catacccccc cctctcctcc catcccccca acccttactg tatttttaca
     6181 acaattacca acaacaacaa acaacaaaca acattacaat tactatttac aattacaatg
     6241 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     6301 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     6361 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     6421 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     6481 gagtttagcg agagcctgac ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa
     6541 gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg
     6601 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     6661 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     6721 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     6781 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     6841 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     6901 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     6961 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccacga
     7021 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     7081 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     7141 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     7201 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa
     7261 tgagcttgtt tgagtattat ggcattggaa aagctgtttt cttctatcat ttgttctgct
     7321 tgtaatttac tgtgttcttt cagtttttgt tttcggacat caaaatgcaa atggatggat
     7381 aagagttaat aaatgatatg gtccttttgt tcattctcaa attattatta tctgttgttt
     7441 ttactttaat gggttgaatt taagtaagaa aggaactaac agtgtgatat taaggtgcaa
     7501 tgttagacat ataaaacagt ctttcacctc tctttggtta tgtcttgaat tggtttgttt
     7561 cttcacttat ctgtgtaatc aagtttacta tgagtctatg atcaagtaat tatgcaatca
     7621 agttaagtac agtataggct ttttgtgtcg acatttattc actttccttt ctgcaatttt
     7681 caaatcatgt ttgtagtttt tattggactt gatgaatctc tcttaccacc aaaatagaac
     7741 attttttctt gatataccag aatctcaata tttgtctgat aattattcta gctgagcgct
     7801 ttactgagac cgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7861 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7921 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7981 ccatgattac gccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga
     8041 gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8101 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8161 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     8221 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     8281 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8341 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacg gtctcaggga
     8401 cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     8461 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     8521 ttcgtccatt tgtatgtgcc agccgccttt gcgacgctca ccgggctggt tgccctcgcc
     8581 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
     8641 gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac
     8701 agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat
     8761 gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa
     8821 aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc
     8881 ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca
     8941 cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc
     9001 acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct
     9061 gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc
     9121 ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc
     9181 cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac
     9241 cccaaaaatg gcagcgctgg ccaattcccg agtgcgcgga acccctattt gtttattttt
     9301 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9361 atattgaaaa aggaagagta tggctaaaat gagaatatca ccggaattga aaaaactgat
     9421 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     9481 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     9541 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     9601 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     9661 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     9721 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     9781 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
     9841 ggattgcgaa aactgggaag aggacactcc atttaaagat ccgcgcgagc tgtatgattt
     9901 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
     9961 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
    10021 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg ctcagggagg atatcgggga
    10081 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
    10141 aaaatattat attttactgg atgaattgtt ttagctgtca gaccaagttt actcatatat
    10201 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10261 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10321 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10381 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10441 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10501 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10561 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10621 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10681 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10741 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10801 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10861 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10921 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcag atcctagatg
    10981 tggcgcaacg atgccggcga caagcaggag cgcaccgact tcttccgcat caagtgtttt
    11041 ggctctcagg ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag
    11101 ggcaagattc ggaataccaa gtacgagaag gacggccaga cggtctacgg gaccgacttc
    11161 attgccgata aggtggatta tctggacacc aaggcaccag gcgggtcaaa tcaggaataa
    11221 gggcacattg ccccggcgtg agtcggggca atcccgcaag gagggtgaat gaatcggacg
    11281 tttgaccgga aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc
    11341 gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc
    11401 tcgatggtcc agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct
    11461 gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc gtcttgaaca ggaggcggca
    11521 ggtttggcga agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa
    11581 accgccggcg aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa
    11641 cacacgaagc agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg
    11701 gacacgatgc gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac
    11761 aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg
    11821 aagatcacct acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag
    11881 gtgttggagt acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag
    11941 ctttgccagg acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc
    12001 ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa
    12061 tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc
    12121 caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc
    12181 atatgggaga agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc
    12241 tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat
    12301 tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc
    12361 agcggcctgg tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa acgctagggc
    12421 cttgtggggt cagttccggc tgggggttca gcagcccctg ctcggatctg ttggaccgga
    12481 cagtagtcat ggttgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    12541 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
    12601 gacaacttaa taacacattg cggacgtttt taatgtactg gggttgaaca ctct
//
