LOCUS       sequence-378126-       11648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the NOR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP769_L2pV1_NOR_B3RT_FRT_Rluc_1IF_lacZ
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          978..1166
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     protein_bind    2193..2226
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2261..2435
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2823..4475
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2889..2907)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4495..4695
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5038..5093
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5101..5121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5805..5993
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    6617..6791
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    6861..6882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6897..6902,6903..6920,6921..6927)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6935..6951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6940..6962
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6959..6975
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6959..6975
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6971..7294
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    6988..7044
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7045..7062)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7045..7061)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7054..7076)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7270..7289)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    7426..7450
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7538..8248
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8269..8373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8374..9168
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9339..9927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9828..9847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10263..11411
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11557..11581
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcgg ttgcttaaga ataagtaatt
      901 cttaagcaac ccaacaaaca acattacaat tactatttac aattacaatg acttcaaagg
      961 tgtatgatcc tgagcaggta agtttctgct tctacctttg atatatatat aataattatc
     1021 attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata
     1081 tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata
     1141 tatgaccaaa atttgttgat gtgcagagaa aaagaatgat aactggtcca caatggtggg
     1201 caagatgtaa acagatgaac gtgcttgatt ctttcattaa ttactacgat tcagaaaagc
     1261 acgctgagaa tgcagttatc tttcttcacg gaaacgctgc atcttcatat ttgtggagac
     1321 atgttgtgcc tcacattgaa ccagtggcta ggtgtattat cccagatctt atcggaatgg
     1381 gaaagtctgg taaatctgga aatggttctt acagactttt ggatcattat aagtacctca
     1441 cagcttggtt cgaacttctc aacttaccta agaagattat tttcgttgga cacgattggg
     1501 gtgcttgctt ggcattccat tactcatacg aacaccaaga taagatcaaa gctatagttc
     1561 atgcagagag tgttgtggat gtgatagaat cttgggatga gtggccagat attgaagagg
     1621 atattgcttt gatcaagtct gaagagggag aaaagatggt tctcgaaaat aactttttcg
     1681 tggaaactat gcttccttca aagattatga gaaaattgga accagaagag tttgctgcat
     1741 atctcgaacc tttcaaggaa aaaggagagg ttagaaggcc aacactcagt tggcctaggg
     1801 aaataccatt agttaaggga ggtaaacctg atgttgtgca gatcgtgaga aattataacg
     1861 cttaccttag ggcatcagat gatttgccta agatgttcat cgagtcagat ccaggatttt
     1921 tcagtaatgc tatagttgaa ggtgcaaaga aatttccaaa caccgagttc gttaaggtga
     1981 aaggattgca tttctcacaa gaagatgctc ctgatgagat gggaaagtat attaagtctt
     2041 ttgttgaaag agtgctcaaa aacgagcagg gttctcgatc tgatgcttct gaagtaacgt
     2101 caacaagctc ccagtctgag gtatgcacag tggaaacgcc aggttgtgtt ggttcttaag
     2161 gttgcttaag aataagtaat tcttaagcaa ccgaagttcc tattctctag aaagtatagg
     2221 aacttcgctt ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     2281 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     2341 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     2401 tcctaaaacc aaaatccagt actaaaatcc agatcgctgc aagaattcaa gcttggagcc
     2461 aacactcgaa tccccacccg ttggaccaaa cccggctcat taagcgtcgg ttcagattta
     2521 tttcctttat ttaaaaaaaa ggaaagggta aaaaatagaa aattggaaac agttaaagcc
     2581 caaaattgta atttaccgag aattgtaaat ttacctgaaa accctacgct atagtttcga
     2641 ctataaatac caaacttagg acctcacttc agaatcccct cgtcgctgcg tctctctccc
     2701 gcaaccttcg attttcgttt attcgcatcc atcggagaga gaaaacaatc aataagcgac
     2761 ctactacaaa caagtaagta agagaaaaac caaaagaaga agagaaacaa caagaagaag
     2821 taatggagga cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg
     2881 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
     2941 ttgcttttac agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt
     3001 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     3061 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     3121 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca
     3181 tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     3241 aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg
     3301 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     3361 attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg
     3421 gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct
     3481 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     3541 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     3601 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt
     3661 acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc
     3721 tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt
     3781 cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat
     3841 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     3901 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     3961 aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg
     4021 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     4081 ctggagacat agcttactgg gacgaggacg aacacttctt catagttgac cgcttgaagt
     4141 ctttaattaa atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac
     4201 aacaccccaa catcttcgac gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac
     4261 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     4321 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     4381 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     4441 taaaggccaa gaagggcgga aagtccaaat tgtaagcttg atcccccgtc gacagctagc
     4501 tatatcatca atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg
     4561 taccagctga tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt
     4621 tatgtttttg cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta
     4681 tatttctttc aagatcgcta ctagaattcg agctcggagc caacactcga atccccaccc
     4741 gttggaccaa acccggctca ttaagcgtcg gttcagattt atttccttta tttaaaaaaa
     4801 aggaaagggt aaaaaataga aaattggaaa cagttaaagc ccaaaattgt aatttaccga
     4861 gaattgtaaa tttacctgaa aaccctacgc tatagtttcg actataaata ccaaacttag
     4921 gacctcactt cagaatcccc tcgtcgctgc gtctctctcc cgcaaccttc gattttcgtt
     4981 tattcgcatc catcggagag agaaaacaat caataagcga cctactgtat ttttacaaca
     5041 attaccaaca acaacaaaca acaaacaaca ttacaattac tatttacaat tacaatggct
     5101 cctaagaaga agaggaaggt gatgagccag ttcgacatcc tgtgcaagac cccccccaag
     5161 gtgctggtgc ggcagttcgt ggagagattc gagaggccca gcggcgagaa gatcgccagc
     5221 tgtgccgccg agctgaccta cctgtgctgg atgatcaccc acaacggcac cgccatcaag
     5281 agggccacct tcatgagcta caacaccatc atcagcaaca gcctgagctt cgacatcgtg
     5341 aacaagagcc tgcagttcaa gtacaagacc cagaaggcca ccatcctgga ggccagcctg
     5401 aagaagctga tccccgcctg ggagttcacc atcatccctt acaacggcca gaagcaccag
     5461 agcgacatca ccgacatcgt gtccagcctg cagctgcagt tcgagagcag cgaggaggcc
     5521 gacaagggca acagccacag caagaagatg ctgaaggccc tgctgtccga gggcgagagc
     5581 atctgggaga tcaccgagaa gatcctgaac agcttcgagt acaccagcag gttcaccaag
     5641 accaagaccc tgtaccagtt cctgttcctg gccacattca tcaactgcgg caggttcagc
     5701 gacatcaaga acgtggaccc caagagcttc aagctggtgc agaacaagta cctgggcgtg
     5761 atcattcagt gcctggtgac cgagactaag acaagcgtgt ccaggtaagt ttctgcttct
     5821 acctttgata tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt
     5881 tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt
     5941 atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggcacatc
     6001 tactttttca gcgccagagg caggatcgac cccctggtgt acctggacga gttcctgagg
     6061 aacagcgagc ccgtgctgaa gagagtgaac aggaccggca acagcagcag caacaagcag
     6121 gagtaccagc tgctgaagga caacctggtg cgcagctaca acaaggccct gaagaagaac
     6181 gccccctacc ccatcttcgc tatcaagaac ggccctaaga gccacatcgg caggcacctg
     6241 atgaccagct ttctgagcat gaagggcctg accgagctga caaacgtggt gggcaactgg
     6301 agcgacaaga gggcctccgc cgtggccagg accacctaca cccaccagat caccgccatc
     6361 cccgaccact acttcgccct ggtgtccagg tactacgcct acgaccccat cagcaaggag
     6421 atgatcgccc tgaaggacga gactaacccc atcgaggagt ggcagcacat cgagcagctg
     6481 aagggcagcg ccgagggcag catcagatac cccgcctgga acggcatcat cagccaggag
     6541 gtgctggact acctgagcag ctacatcaac aggcggatct gagcttctct agctagagtc
     6601 gatcgacaag ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta
     6661 gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg
     6721 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta
     6781 aaatccagat cgctttactg agaccgcagc tggcacgaca ggtttcccga ctggaaagcg
     6841 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     6901 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6961 ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga
     7021 tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7081 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7141 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7201 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     7261 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     7321 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     7381 gacggtctca gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg
     7441 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     7501 aaaaggttta tccgttcgtc catttgtatg tgccagccgc ctttgcgacg ctcaccgggc
     7561 tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc
     7621 cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc gcggaaaact
     7681 tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg
     7741 cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct
     7801 cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc
     7861 tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg
     7921 cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat
     7981 ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg
     8041 gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta
     8101 accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc
     8161 gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg cccctcggcc
     8221 gcgaacggcc tcaccccaaa aatggcagcg ctggccaatt cccgagtgcg cggaacccct
     8281 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8341 taaatgcttc aataatattg aaaaaggaag agtatggcta aaatgagaat atcaccggaa
     8401 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
     8461 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
     8521 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
     8581 aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc
     8641 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
     8701 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
     8761 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
     8821 gatctggccg atgtggattg cgaaaactgg gaagaggaca ctccatttaa agatccgcgc
     8881 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
     8941 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
     9001 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgctcagg
     9061 gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
     9121 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagct gtcagaccaa
     9181 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9241 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9301 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9361 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9421 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9481 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9541 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9601 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9661 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9721 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9781 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9841 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9901 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9961 gcagatccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc gacttcttcc
    10021 gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc aaggggtcgc
    10081 tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct
    10141 acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt
    10201 caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt
    10261 gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt
    10321 ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct
    10381 tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc
    10441 aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtcttg
    10501 aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga
    10561 ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg
    10621 ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata
    10681 ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt
    10741 tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg
    10801 tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac
    10861 tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct
    10921 tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga
    10981 aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg
    11041 ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga
    11101 aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc
    11161 actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt
    11221 tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca
    11281 tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg
    11341 aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt
    11401 gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc cctgctcgga
    11461 tctgttggac cggacagtag tcatggttga tgggctgcct gtatcgagtg gtgattttgt
    11521 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    11581 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actggggttg
    11641 aacactct
//
