LOCUS       sequence-378129-       12077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the NOR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP770_L2pV1_NOR_B3RT_FRT_Rluc_1IB_lacZ
FEATURES             Location/Qualifiers
     source          1..12077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          978..1166
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     protein_bind    2193..2226
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2261..2435
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2823..4475
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2889..2907)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4495..4695
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5038..5093
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5098..5118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5896..6084
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7046..7220
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7290..7311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7326..7331,7332..7349,7350..7356)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7364..7380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7369..7391
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7388..7404
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7388..7404
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7400..7723
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7417..7473
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7474..7491)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7474..7490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7483..7505)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7699..7718)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    7855..7879
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7967..8677
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8698..8802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8803..9597
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9768..10356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10257..10276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10692..11840
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11986..12010
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcgg ttgcttaaga ataagtaatt
      901 cttaagcaac ccaacaaaca acattacaat tactatttac aattacaatg acttcaaagg
      961 tgtatgatcc tgagcaggta agtttctgct tctacctttg atatatatat aataattatc
     1021 attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata
     1081 tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata
     1141 tatgaccaaa atttgttgat gtgcagagaa aaagaatgat aactggtcca caatggtggg
     1201 caagatgtaa acagatgaac gtgcttgatt ctttcattaa ttactacgat tcagaaaagc
     1261 acgctgagaa tgcagttatc tttcttcacg gaaacgctgc atcttcatat ttgtggagac
     1321 atgttgtgcc tcacattgaa ccagtggcta ggtgtattat cccagatctt atcggaatgg
     1381 gaaagtctgg taaatctgga aatggttctt acagactttt ggatcattat aagtacctca
     1441 cagcttggtt cgaacttctc aacttaccta agaagattat tttcgttgga cacgattggg
     1501 gtgcttgctt ggcattccat tactcatacg aacaccaaga taagatcaaa gctatagttc
     1561 atgcagagag tgttgtggat gtgatagaat cttgggatga gtggccagat attgaagagg
     1621 atattgcttt gatcaagtct gaagagggag aaaagatggt tctcgaaaat aactttttcg
     1681 tggaaactat gcttccttca aagattatga gaaaattgga accagaagag tttgctgcat
     1741 atctcgaacc tttcaaggaa aaaggagagg ttagaaggcc aacactcagt tggcctaggg
     1801 aaataccatt agttaaggga ggtaaacctg atgttgtgca gatcgtgaga aattataacg
     1861 cttaccttag ggcatcagat gatttgccta agatgttcat cgagtcagat ccaggatttt
     1921 tcagtaatgc tatagttgaa ggtgcaaaga aatttccaaa caccgagttc gttaaggtga
     1981 aaggattgca tttctcacaa gaagatgctc ctgatgagat gggaaagtat attaagtctt
     2041 ttgttgaaag agtgctcaaa aacgagcagg gttctcgatc tgatgcttct gaagtaacgt
     2101 caacaagctc ccagtctgag gtatgcacag tggaaacgcc aggttgtgtt ggttcttaag
     2161 gttgcttaag aataagtaat tcttaagcaa ccgaagttcc tattctctag aaagtatagg
     2221 aacttcgctt ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     2281 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     2341 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     2401 tcctaaaacc aaaatccagt actaaaatcc agatcgctgc aagaattcaa gcttggagcc
     2461 aacactcgaa tccccacccg ttggaccaaa cccggctcat taagcgtcgg ttcagattta
     2521 tttcctttat ttaaaaaaaa ggaaagggta aaaaatagaa aattggaaac agttaaagcc
     2581 caaaattgta atttaccgag aattgtaaat ttacctgaaa accctacgct atagtttcga
     2641 ctataaatac caaacttagg acctcacttc agaatcccct cgtcgctgcg tctctctccc
     2701 gcaaccttcg attttcgttt attcgcatcc atcggagaga gaaaacaatc aataagcgac
     2761 ctactacaaa caagtaagta agagaaaaac caaaagaaga agagaaacaa caagaagaag
     2821 taatggagga cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg
     2881 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
     2941 ttgcttttac agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt
     3001 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     3061 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     3121 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca
     3181 tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     3241 aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg
     3301 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     3361 attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg
     3421 gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct
     3481 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     3541 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     3601 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt
     3661 acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc
     3721 tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt
     3781 cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat
     3841 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     3901 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     3961 aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg
     4021 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     4081 ctggagacat agcttactgg gacgaggacg aacacttctt catagttgac cgcttgaagt
     4141 ctttaattaa atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac
     4201 aacaccccaa catcttcgac gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac
     4261 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     4321 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     4381 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     4441 taaaggccaa gaagggcgga aagtccaaat tgtaagcttg atcccccgtc gacagctagc
     4501 tatatcatca atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg
     4561 taccagctga tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt
     4621 tatgtttttg cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta
     4681 tatttctttc aagatcgcta ctagaattcg agctcggagc caacactcga atccccaccc
     4741 gttggaccaa acccggctca ttaagcgtcg gttcagattt atttccttta tttaaaaaaa
     4801 aggaaagggt aaaaaataga aaattggaaa cagttaaagc ccaaaattgt aatttaccga
     4861 gaattgtaaa tttacctgaa aaccctacgc tatagtttcg actataaata ccaaacttag
     4921 gacctcactt cagaatcccc tcgtcgctgc gtctctctcc cgcaaccttc gattttcgtt
     4981 tattcgcatc catcggagag agaaaacaat caataagcga cctactgtat ttttacaaca
     5041 attaccaaca acaacaaaca acaaacaaca ttacaattac tatttacaat tacaatgccc
     5101 aagaaaaagc ggaaggtgtc cagctacatg gacctggtgg acgacgagcc cgccaccctg
     5161 taccacaagt tcgtggaatg cctgaaggcc ggcgagaact tctgcggcga taagctgagc
     5221 ggcatcatca ccatggccat tctgaaggcc atcaaggccc tgaccgaagt gaagaaaacc
     5281 accttcaaca agtacaagac caccatcaag cagggcctgc agtacgacgt gggcagcagc
     5341 accatcagct tcgtgtacca cctgaaggac tgcgacgagc tgagcagagg cctgagcgac
     5401 gccttcgagc cctacaagtt caagatcaag agcaacaaag aggccaccag cttcaagacc
     5461 ctgttcaggg gccctagctt cggcagccag aagaactggc ggaagaaaga ggtggaccgc
     5521 gaggtggaca acctgttcca cagcaccgag acagacgaga gcatcttcaa gttcatcctg
     5581 aacaccctgg acagcatcga aacccagacc aacaccgacc ggcagaaaac cgtgctgacc
     5641 tttatcctgc tgatgacctt cttcaactgc tgccggaaca acgacctgat gaacgtggac
     5701 cccagcacct tcaagatcgt gaagaacaag tttgtgggct acctgctgca ggctgaagtg
     5761 aagcagacca agaccagaaa gagccggaat atcttcttct tccccatccg ggaaaaccgc
     5821 ttcgacctgt tcctggccct gcacgacttc ttcagaacct gccagcccac ccccaagagc
     5881 agactgagcg atcaggtaag tttctgcttc tacctttgat atatatataa taattatcat
     5941 taattagtag taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata
     6001 gcaattgctt ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata
     6061 tgaccaaaat ttgttgatgt gcaggtgtcc gagcagaagt ggcagctgtt ccgggacagc
     6121 atggtcatcg actacaaccg gttctttcgg aagttccccg ccagccccat cttcgccatt
     6181 aagcacggcc ccaagtccca cctgggccgg catctgatga acagctttct gcacaagaac
     6241 gagctggaca gctgggccaa cagcctgggc aattggagca gctcccagaa ccagagagag
     6301 agcggcgcca gactgggcta cacacacggc ggaagagatc tgccccagcc cctgtttggc
     6361 ttcctggccg gatactgcgt gcggaacgaa gagggccaca tcgtgggcct gggcctggaa
     6421 aaggacatca acgatctgtt cgacggcatc atggaccccc tgaacgagaa agaggacacc
     6481 gagatctgcg agagctacgg cgagtgggcc aagattgtgt ccaaggacgt gctgatcttc
     6541 ctgaagagat accacagcaa gaacgcctgt cggagatacc agaacagcac cctgtatgcc
     6601 cggaccttcc tgaaaaccga gagcgtgacc ctgagcggct ccaagggcag cgaggaacct
     6661 tctagccctg tgcggatccc catcctgagc atgggaaagg ccagcccctc cgagggaaga
     6721 aagctgagag ccagcgagca cgccaacgac gacaacgaga tcgagaagat cgacagcgac
     6781 agcagccaga gcgaagagat ccctatcgag atgagcgact ccgaggacga gacaaccgcc
     6841 agcaacatca gcggcatcta cctggacatg agcaaggcca actccaacgt ggtgtacagc
     6901 ccccctagcc agacaggcag agctgctggc gccggaagaa aaagaggcgt gggaggcaga
     6961 cggaccgtgg aaagcaagcg gagaagagtg ctggccccca tcaaccggtg agcttctcta
     7021 gctagagtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata
     7081 agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt
     7141 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     7201 ccagtactaa aatccagatc gctttactga gaccgcagct ggcacgacag gtttcccgac
     7261 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7321 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7381 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac
     7441 tctagaggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga
     7501 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     7561 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     7621 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     7681 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     7741 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     7801 acaagctgtg acggtctcag ggacacgaag tgatccgttt aaactatcag tgtttgacag
     7861 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     7921 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gccagccgcc tttgcgacgc
     7981 tcaccgggct ggttgccctc gccgctgggc tggcggccgt ctatggccct gcaaacgcgc
     8041 cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg ccggcgttgt ggatacctcg
     8101 cggaaaactt ggccctcact gacagatgag gggcggacgt tgacacttga ggggccgact
     8161 cacccggcgc ggcgttgaca gatgaggggc aggctcgatt tcggccggcg acgtggagct
     8221 ggccagcctc gcaaatcggc gaaaacgcct gattttacgc gagtttccca cagatgatgt
     8281 ggacaagcct ggggataagt gccctgcggt attgacactt gaggggcgcg actactgaca
     8341 gatgaggggc gcgatccttg acacttgagg ggcagagtgc tgacagatga ggggcgcacc
     8401 tattgacatt tgaggggctg tccacaggca gaaaatccag catttgcaag ggtttccgcc
     8461 cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt taaaccaata tttataaacc
     8521 ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc
     8581 ccccttctcg aaccctcccg gcccgctaac gcgggcctcc catcccccca ggggctgcgc
     8641 ccctcggccg cgaacggcct caccccaaaa atggcagcgc tggccaattc ccgagtgcgc
     8701 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8761 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatggctaa aatgagaata
     8821 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     8881 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     8941 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     9001 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     9061 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     9121 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     9181 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     9241 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaggacac tccatttaaa
     9301 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     9361 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     9421 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     9481 tcgctcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     9541 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagctg
     9601 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9661 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9721 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9781 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9841 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9901 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9961 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10021 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10081 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10141 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10201 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10261 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10321 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10381 cggttcctgg cagatcctag atgtggcgca acgatgccgg cgacaagcag gagcgcaccg
    10441 acttcttccg catcaagtgt tttggctctc aggccgaggc ccacggcaag tatttgggca
    10501 aggggtcgct ggtattcgtg cagggcaaga ttcggaatac caagtacgag aaggacggcc
    10561 agacggtcta cgggaccgac ttcattgccg ataaggtgga ttatctggac accaaggcac
    10621 caggcgggtc aaatcaggaa taagggcaca ttgccccggc gtgagtcggg gcaatcccgc
    10681 aaggagggtg aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg
    10741 cggggttttc cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc
    10801 gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc tacggccaag atcgagcgcg
    10861 acagcgtgca actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc
    10921 gtcgtcttga acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa
    10981 ctatgacgac caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg
    11041 ccaagcaggc cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct
    11101 tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct
    11161 ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca
    11221 ttttccacgt caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg
    11281 atgacgaact ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct atcggcgagc
    11341 cgatcacctt cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt
    11401 attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt
    11461 ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc
    11521 gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg
    11581 ctggcgacca ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc
    11641 gacggatgtt cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct
    11701 tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg
    11761 aagcctgcga agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc
    11821 tggtgcattg caaacgctag ggccttgtgg ggtcagttcc ggctgggggt tcagcagccc
    11881 ctgctcggat ctgttggacc ggacagtagt catggttgat gggctgcctg tatcgagtgg
    11941 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
    12001 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
    12061 ctggggttga acactct
//
