LOCUS       sequence-37813-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: JDS70
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             74..139
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             146..166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
       61 ggctagcacc atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta
      121 caaggatgac gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg
      181 ggtacctatg gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa
      241 gcctaaggtc aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac
      301 tcatgcgcat attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa
      361 ataccaagat atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg
      421 taaacagtgg tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag
      481 ggggcctccg ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt
      541 aacagcggta gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacag
      601 agacgattaa tgcgtctcgc tgacacccga acaggtggtc gccattgctt ctaacatcgg
      661 aggacggcca gccttggagt ccatcgtagc ccaattgtcc aggcccgatc ccgcgttggc
      721 tgcgttaacg aatgaccatc tggtggcgtt ggcatgtctt ggtggacgac ccgcgctcga
      781 tgcagtcaaa aagggtctgc ctcatgctcc cgcattgatc aaaagaacca accggcggat
      841 tcccgagaga acttcccatc gagtcgcggg atcccaacta gtcaaaagtg aactggagga
      901 g
//
