LOCUS       sequence-378131-       14057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the 2-input state of the OR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14057)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 14057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre
FEATURES             Location/Qualifiers
     source          1..14057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(879..912)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      913..1620
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1621..1654
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(1655..1688)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          1755..1943
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2971..3145
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3533..5185
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3599..3617)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5205..5405
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5748..5803
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5811..5831
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6515..6703
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7327..7501
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        7602..7947
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7890..7910
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8044..8064
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8494..8682
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     misc_feature    9835..9859
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9947..10657
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10678..10782
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10783..11577
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11748..12336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12237..12256
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12672..13820
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13966..13990
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcat aacttcgtat agcatacatt
      901 atacgaagtt atctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct
      961 ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc
     1021 cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata
     1081 ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa
     1141 caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa
     1201 caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct
     1261 aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta
     1321 cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc
     1381 gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg
     1441 gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta
     1501 tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg
     1561 acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag
     1621 gaagttccta ttctctagaa agtataggaa cttcataact tcgtatagca tacattatac
     1681 gaagttatca acaaacaaca ttacaattac tatttacaat tacaatgact tcaaaggtgt
     1741 atgatcctga gcaggtaagt ttctgcttct acctttgata tatatataat aattatcatt
     1801 aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag
     1861 caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat
     1921 gaccaaaatt tgttgatgtg cagagaaaaa gaatgataac tggtccacaa tggtgggcaa
     1981 gatgtaaaca gatgaacgtg cttgattctt tcattaatta ctacgattca gaaaagcacg
     2041 ctgagaatgc agttatcttt cttcacggaa acgctgcatc ttcatatttg tggagacatg
     2101 ttgtgcctca cattgaacca gtggctaggt gtattatccc agatcttatc ggaatgggaa
     2161 agtctggtaa atctggaaat ggttcttaca gacttttgga tcattataag tacctcacag
     2221 cttggttcga acttctcaac ttacctaaga agattatttt cgttggacac gattggggtg
     2281 cttgcttggc attccattac tcatacgaac accaagataa gatcaaagct atagttcatg
     2341 cagagagtgt tgtggatgtg atagaatctt gggatgagtg gccagatatt gaagaggata
     2401 ttgctttgat caagtctgaa gagggagaaa agatggttct cgaaaataac tttttcgtgg
     2461 aaactatgct tccttcaaag attatgagaa aattggaacc agaagagttt gctgcatatc
     2521 tcgaaccttt caaggaaaaa ggagaggtta gaaggccaac actcagttgg cctagggaaa
     2581 taccattagt taagggaggt aaacctgatg ttgtgcagat cgtgagaaat tataacgctt
     2641 accttagggc atcagatgat ttgcctaaga tgttcatcga gtcagatcca ggatttttca
     2701 gtaatgctat agttgaaggt gcaaagaaat ttccaaacac cgagttcgtt aaggtgaaag
     2761 gattgcattt ctcacaagaa gatgctcctg atgagatggg aaagtatatt aagtcttttg
     2821 ttgaaagagt gctcaaaaac gagcagggtt ctcgatctga tgcttctgaa gtaacgtcaa
     2881 caagctccca gtctgaggta tgcacagtgg aaacgccagg ttgtgttggt tcttaagctt
     2941 ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc
     3001 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     3061 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     3121 aaaatccagt actaaaatcc agatcgctgc aagaattcaa gcttggagcc aacactcgaa
     3181 tccccacccg ttggaccaaa cccggctcat taagcgtcgg ttcagattta tttcctttat
     3241 ttaaaaaaaa ggaaagggta aaaaatagaa aattggaaac agttaaagcc caaaattgta
     3301 atttaccgag aattgtaaat ttacctgaaa accctacgct atagtttcga ctataaatac
     3361 caaacttagg acctcacttc agaatcccct cgtcgctgcg tctctctccc gcaaccttcg
     3421 attttcgttt attcgcatcc atcggagaga gaaaacaatc aataagcgac ctactacaaa
     3481 caagtaagta agagaaaaac caaaagaaga agagaaacaa caagaagaag taatggagga
     3541 cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg
     3601 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     3661 agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt
     3721 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     3781 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     3841 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc
     3901 taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt
     3961 accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     4021 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc
     4081 agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg
     4141 gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag
     4201 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     4261 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     4321 cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca
     4381 aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa
     4441 atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt
     4501 cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac
     4561 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     4621 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     4681 cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa
     4741 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     4801 agcttactgg gacgaggacg aacacttctt catagttgac cgcttgaagt ctttaattaa
     4861 atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa
     4921 catcttcgac gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac ttcccgccgc
     4981 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     5041 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     5101 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     5161 gaagggcgga aagtccaaat tgtaagcttg atcccccgtc gacagctagc tatatcatca
     5221 atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga
     5281 tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg
     5341 cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc
     5401 aagatcgcta ctagaattcg agctcggagc caacactcga atccccaccc gttggaccaa
     5461 acccggctca ttaagcgtcg gttcagattt atttccttta tttaaaaaaa aggaaagggt
     5521 aaaaaataga aaattggaaa cagttaaagc ccaaaattgt aatttaccga gaattgtaaa
     5581 tttacctgaa aaccctacgc tatagtttcg actataaata ccaaacttag gacctcactt
     5641 cagaatcccc tcgtcgctgc gtctctctcc cgcaaccttc gattttcgtt tattcgcatc
     5701 catcggagag agaaaacaat caataagcga cctactgtat ttttacaaca attaccaaca
     5761 acaacaaaca acaaacaaca ttacaattac tatttacaat tacaatggct cctaagaaga
     5821 agaggaaggt gatgagccag ttcgacatcc tgtgcaagac cccccccaag gtgctggtgc
     5881 ggcagttcgt ggagagattc gagaggccca gcggcgagaa gatcgccagc tgtgccgccg
     5941 agctgaccta cctgtgctgg atgatcaccc acaacggcac cgccatcaag agggccacct
     6001 tcatgagcta caacaccatc atcagcaaca gcctgagctt cgacatcgtg aacaagagcc
     6061 tgcagttcaa gtacaagacc cagaaggcca ccatcctgga ggccagcctg aagaagctga
     6121 tccccgcctg ggagttcacc atcatccctt acaacggcca gaagcaccag agcgacatca
     6181 ccgacatcgt gtccagcctg cagctgcagt tcgagagcag cgaggaggcc gacaagggca
     6241 acagccacag caagaagatg ctgaaggccc tgctgtccga gggcgagagc atctgggaga
     6301 tcaccgagaa gatcctgaac agcttcgagt acaccagcag gttcaccaag accaagaccc
     6361 tgtaccagtt cctgttcctg gccacattca tcaactgcgg caggttcagc gacatcaaga
     6421 acgtggaccc caagagcttc aagctggtgc agaacaagta cctgggcgtg atcattcagt
     6481 gcctggtgac cgagactaag acaagcgtgt ccaggtaagt ttctgcttct acctttgata
     6541 tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa
     6601 taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat
     6661 ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggcacatc tactttttca
     6721 gcgccagagg caggatcgac cccctggtgt acctggacga gttcctgagg aacagcgagc
     6781 ccgtgctgaa gagagtgaac aggaccggca acagcagcag caacaagcag gagtaccagc
     6841 tgctgaagga caacctggtg cgcagctaca acaaggccct gaagaagaac gccccctacc
     6901 ccatcttcgc tatcaagaac ggccctaaga gccacatcgg caggcacctg atgaccagct
     6961 ttctgagcat gaagggcctg accgagctga caaacgtggt gggcaactgg agcgacaaga
     7021 gggcctccgc cgtggccagg accacctaca cccaccagat caccgccatc cccgaccact
     7081 acttcgccct ggtgtccagg tactacgcct acgaccccat cagcaaggag atgatcgccc
     7141 tgaaggacga gactaacccc atcgaggagt ggcagcacat cgagcagctg aagggcagcg
     7201 ccgagggcag catcagatac cccgcctgga acggcatcat cagccaggag gtgctggact
     7261 acctgagcag ctacatcaac aggcggatct gagcttctct agctagagtc gatcgacaag
     7321 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat
     7381 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     7441 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
     7501 cgctttacga attcccatgg ggaggtcaac atggtggagc acgacactct ggtctactcc
     7561 aaaaatgtca aagatacagt ctcagaagat caaagggcta ttgagacttt tcaacaaagg
     7621 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     7681 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     7741 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     7801 gtggaaaaag aagaggttcc aaccacgtct acaaagcaag tggattgatg tgacatctcc
     7861 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     7921 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctcattt ttacaacaat
     7981 taccaacaac aacaaacaac aaacaacatt acaattacat ttacaattat cgatacaatg
     8041 gtgcccaaga agaagaggaa agtctccaac ctgctgactg tgcaccaaaa cctgcctgcc
     8101 ctccctgtgg atgccacctc tgatgaagtc aggaagaacc tgatggacat gttcagggac
     8161 aggcaggcct tctctgaaca cacctggaag atgctcctgt ctgtgtgcag atcctgggct
     8221 gcctggtgca agctgaacaa caggaaatgg ttccctgctg aacctgagga tgtgagggac
     8281 tacctcctgt acctgcaagc cagaggcctg gctgtgaaaa ccatccaaca gcacctgggc
     8341 cagctcaaca tgctgcacag gagatctggc ctgcctcgcc cttctgactc caatgctgtg
     8401 tccctggtga tgaggagaat cagaaaggag aatgtggatg ctggggagag agccaagcag
     8461 gccctggcct ttgaacgcac tgactttgac caggtaagtt tctgcttcta cctttgatat
     8521 atatataata attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat
     8581 aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt
     8641 tataactttt ctaatatatg accaaaattt gttgatgtgc aggtcagatc cctgatggag
     8701 aactctgaca gatgccagga catcaggaac ctggccttcc tgggcattgc ctacaacacc
     8761 ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg acatctcccg caccgatggt
     8821 gggagaatgc tgatccacat tggcaggacc aagaccctgg tgtccacagc tggtgtggag
     8881 aaggccctgt ccctgggggt taccaagctg gtggagagat ggatctctgt gtctggtgtg
     8941 gctgatgacc ccaacaacta cctgttctgc cgggtcagaa agaatggtgt ggctgcccct
     9001 tctgccacct cccaactgtc cacccgggcc ctggaaggga tctttgaggc cacccaccgc
     9061 ctgatctatg gtgccaagga tgactctggg cagagatacc tggcctggtc tggccactct
     9121 gccagagtgg gtgctgccag ggacatggcc agggctggtg tgtccatccc tgaaatcatg
     9181 caggctggtg gctggaccaa tgtgaacatt gtgatgaact acatcagaaa cctggactct
     9241 gagactgggg ccatggtgag gctgctcgag gatggggact gagcttgctc tcaagatcaa
     9301 aggcttaaaa agctggggtt ttatgaatgg gatcaaagtt tctttttttc ttttatattt
     9361 gcttctccat ttgtttgttt catttccctt tttgttttcg tttctatgat gcacttgtgt
     9421 gtgacaaact ctctgggttt ttacttacgt ctgcgtttca aaaaaaaaaa ccgctttcgt
     9481 tttgcgtttt agtcccattg ttttgtagct ctgagtgatc gaattgatgc ctctttattc
     9541 cttttgttcc ctataatttc tttcaaaact cagaagaaaa accttgaaac tctttgcaat
     9601 gttaatataa gtattgtata agatttttat tgatttggtt attagtctta cttttgctac
     9661 ctccatcttc acttggaact gatattctga atagttaaag cgttacatgt gttccattca
     9721 caaatgaact taaactagca caaagtcaga tattttaaga tcgcaccatt tcgctcagag
     9781 aggatgcaca tgtgaccgag ggacacgaag tgatccgttt aaactatcag tgtttgacag
     9841 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     9901 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gccagccgcc tttgcgacgc
     9961 tcaccgggct ggttgccctc gccgctgggc tggcggccgt ctatggccct gcaaacgcgc
    10021 cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg ccggcgttgt ggatacctcg
    10081 cggaaaactt ggccctcact gacagatgag gggcggacgt tgacacttga ggggccgact
    10141 cacccggcgc ggcgttgaca gatgaggggc aggctcgatt tcggccggcg acgtggagct
    10201 ggccagcctc gcaaatcggc gaaaacgcct gattttacgc gagtttccca cagatgatgt
    10261 ggacaagcct ggggataagt gccctgcggt attgacactt gaggggcgcg actactgaca
    10321 gatgaggggc gcgatccttg acacttgagg ggcagagtgc tgacagatga ggggcgcacc
    10381 tattgacatt tgaggggctg tccacaggca gaaaatccag catttgcaag ggtttccgcc
    10441 cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt taaaccaata tttataaacc
    10501 ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc
    10561 ccccttctcg aaccctcccg gcccgctaac gcgggcctcc catcccccca ggggctgcgc
    10621 ccctcggccg cgaacggcct caccccaaaa atggcagcgc tggccaattc ccgagtgcgc
    10681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    10741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatggctaa aatgagaata
    10801 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
    10861 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
    10921 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
    10981 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
    11041 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
    11101 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
    11161 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
    11221 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaggacac tccatttaaa
    11281 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
    11341 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
    11401 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
    11461 tcgctcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
    11521 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagctg
    11581 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    11641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    11701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    11761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    11821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    11881 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    11941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    12001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    12061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    12121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    12181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    12241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    12301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    12361 cggttcctgg cagatcctag atgtggcgca acgatgccgg cgacaagcag gagcgcaccg
    12421 acttcttccg catcaagtgt tttggctctc aggccgaggc ccacggcaag tatttgggca
    12481 aggggtcgct ggtattcgtg cagggcaaga ttcggaatac caagtacgag aaggacggcc
    12541 agacggtcta cgggaccgac ttcattgccg ataaggtgga ttatctggac accaaggcac
    12601 caggcgggtc aaatcaggaa taagggcaca ttgccccggc gtgagtcggg gcaatcccgc
    12661 aaggagggtg aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg
    12721 cggggttttc cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc
    12781 gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc tacggccaag atcgagcgcg
    12841 acagcgtgca actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc
    12901 gtcgtcttga acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa
    12961 ctatgacgac caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg
    13021 ccaagcaggc cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct
    13081 tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct
    13141 ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca
    13201 ttttccacgt caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg
    13261 atgacgaact ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct atcggcgagc
    13321 cgatcacctt cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt
    13381 attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt
    13441 ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc
    13501 gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg
    13561 ctggcgacca ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc
    13621 gacggatgtt cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct
    13681 tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg
    13741 aagcctgcga agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc
    13801 tggtgcattg caaacgctag ggccttgtgg ggtcagttcc ggctgggggt tcagcagccc
    13861 ctgctcggat ctgttggacc ggacagtagt catggttgat gggctgcctg tatcgagtgg
    13921 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
    13981 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
    14041 ctggggttga acactct
//
