LOCUS       sequence-37816-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: JDS71
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             65..130
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             137..157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagcac
       61 catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
      121 cgatgacaag atggccccca agaagaagag gaaggtgggc attcaccgcg gggtacctat
      181 ggtggacttg aggacactcg gttattcgca acagcaacag gagaaaatca agcctaaggt
      241 caggagcacc gtcgcgcaac accacgaggc gcttgtgggg catggcttca ctcatgcgca
      301 tattgtcgcg ctttcacagc accctgcggc gcttgggacg gtggctgtca aataccaaga
      361 tatgattgcg gccctgcccg aagccacgca cgaggcaatt gtaggggtcg gtaaacagtg
      421 gtcgggagcg cgagcacttg aggcgctgct gactgtggcg ggtgagctta gggggcctcc
      481 gctccagctc gacaccgggc agctgctgaa gatcgcgaag agagggggag taacagcggt
      541 agaggcagtg cacgcctggc gcaatgcgct caccggggcc cccttgaaca gagacgatta
      601 atgcgtctcg ctgacacccg aacaggtggt cgccattgct tcccacgacg gaggacggcc
      661 agccttggag tccatcgtag cccaattgtc caggcccgat cccgcgttgg ctgcgttaac
      721 gaatgaccat ctggtggcgt tggcatgtct tggtggacga cccgcgctcg atgcagtcaa
      781 aaagggtctg cctcatgctc ccgcattgat caaaagaacc aaccggcgga ttcccgagag
      841 aacttcccat cgagtcgcgg gatccc
//
