LOCUS       sequence-378215-         539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A FRET-based calcium probe using Sumire.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Sugiura K, Nagai T
  TITLE     Extension of the short wavelength side of fluorescent proteins using
            hydrated chromophores, and its application.
  JOURNAL   Commun Biol. 2022 Nov 3;5(1):1172. doi: 10.1038/s42003-022-04153-7.
  PUBMED    36329112
REFERENCE   2  (bases 1 to 539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-3"; volume: "5(1):1172. doi"; pages: " 
            10.1038/s42003-022-04153-7"
COMMENT     Sequence Label: vgCam-pRSETb
FEATURES             Location/Qualifiers
     source          1..539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(148..169)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatccaa ccatggtgag caagggcgag
      121 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      181 aagttcagcg tgcgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag
      241 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggt
      301 tacggcgtgg cgtgcttcag ccgctacccc gaccacatga agcgccacga cttcttcaag
      361 tccgccatgc ccgaaggcta cgtccaggag cgcaccatca gcttcaagga cgacggcacc
      421 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      481 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacgg
//
