LOCUS       sequence-378218-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A FRET-based calcium probe using Sumire.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Sugiura K, Nagai T
  TITLE     Extension of the short wavelength side of fluorescent proteins using
            hydrated chromophores, and its application.
  JOURNAL   Commun Biol. 2022 Nov 3;5(1):1172. doi: 10.1038/s42003-022-04153-7.
  PUBMED    36329112
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-3"; volume: "5(1):1172. doi"; pages: " 
            10.1038/s42003-022-04153-7"
COMMENT     Sequence Label: vgCam-pRSETb
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(101..124)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     330..349
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     589..610
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccgtttagag gccccaaggg gttatgctag ttattgctna gcggtggcag cagccaactc
       61 agcttccttt cgggctttgt tagcagccgg atcaagctta tttttcgaac tgcgggtggc
      121 tccagcctcc gaattcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
      181 tctgcttgtc ggccatgata tagacgttgt ggctgttgaa gttgtactcc agcttgtgcc
      241 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      301 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      361 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      421 tgtggtcggg gtagcggncg aacaccatca cgccgtanga gaaggtggtc acgagggtgg
      481 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtang
      541 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
      601 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatgccac
      661 cgctgccacc cttgt
//
