LOCUS       sequence-378222-        1198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ratiometric YDA sensor for in vivo cre-dependent neuron-specific 
            cholesterol visualization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Czernecki C, Dixit S, Riezman I, Innocenti S, Bornmann C, Pfrieger 
            FW, Riezman H, Scheiffele P
  TITLE     Cell type-specific assessment of cholesterol distribution in models 
            of neurodevelopmental disorders
  JOURNAL   bioRxiv 2022.11.16.516849
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.11.16.516849"
COMMENT     Sequence Label: pAAV-Syn-DiO-eGFP-2A-mCherry_YDA
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(191..224)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(275..308)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(697..713)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(697..713)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(989..1008)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgncgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
       61 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      121 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctctagagt
      181 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg
      241 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac
      301 gaagttatgg cgcgatcgct agcctacatg gagtgcttga gctccaccgc ggtggcggcc
      361 gcttaattgt aagtaatact agatccaggg tataaagttg ttccccatat tgaaacattt
      421 atattatttg ttaatggaac atcatattca cttataacat ctctccacca ttcccaccaa
      481 aggcctgtac actctcttgc ttttattctt atatttcttg cattagcttc aagaggtatt
      541 actgttgaat agtgagctgt tttccattga taatttccat cccatgtttt atgagttaaa
      601 acttcatttc cttctttgtc agctgaaact tcatcccagg ctacttcaaa ctgtgcaaca
      661 taggctccac tatgatctaa gtttattttt cccttggatc cactagttct agacttgtac
      721 agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg
      781 gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc
      841 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg
      901 tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc
      961 cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
     1021 aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc
     1081 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccna cttgaagccc
     1141 tcggggaagg acagcttcag tagtcgggga tgtcggcggg tgcttccgta ggccttgg
//
