LOCUS       sequence-378226-        1215 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ratiometric D4H sensor for in vivo cre-dependent neuron-specific 
            cholesterol visualization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Czernecki C, Dixit S, Riezman I, Innocenti S, Bornmann C, Pfrieger 
            FW, Riezman H, Scheiffele P
  TITLE     Cell type-specific assessment of cholesterol distribution in models 
            of neurodevelopmental disorders
  JOURNAL   bioRxiv 2022.11.16.516849
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.11.16.516849"
COMMENT     Sequence Label: pAAV-Syn-DiO-eGFP-2A-mCherry_D4H
FEATURES             Location/Qualifiers
     source          1..1215
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(188..221)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(272..305)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(694..710)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(694..710)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(986..1005)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc gcgagatagg ggggcacggg
       61 cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc
      121 ggaggagtcg tgtcgtgcct gagagcgcag tcgagaaggt accggatcct ctagagtcga
      181 ctccggaata acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt
      241 gtagctgcta ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa
      301 gttatggcgc gatcgctagc ctacatggag tgcttgagct ccaccgcggt ggcggccgct
      361 taattgtaag taatactaga tccagggtat aaagttgttc cccatattga aacatttata
      421 ttatttgtta atggaacatc atattcactt ataacatctc tccaccattc ccaagcaagg
      481 cctgtacact ctcttgcttt tattcttata tttcttgcat tagcttcaag aggtattact
      541 gttgaatagt gagctgtttt agattgataa tttccatccc atgttttatg agttaaaact
      601 tcatttcctt ctttgtcata tgaaacttca tcccaggcta cttcaaactg tgcaacatag
      661 gctccactat gatctaagtt tatttttccc ttggatccac tagttctaga cttgtacagc
      721 tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg
      781 tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc
      841 tgcacgggct tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc
      901 ttcagcttca gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc
      961 tcggaggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag
     1021 ttggtgccgc gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggartcctgg
     1081 gtcacggtca ccacgccgcc gtcctcgaag ttcatcmcgc gctccccttg aagccctcgg
     1141 ggaaggaagc ttcagtagtc gggatgtcgg cggggtgctt cacgtaggcc ttgganccgt
     1201 actgaactga gggga
//
