LOCUS       sequence-37823-e       12592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12592)
  AUTHORS   Benzekhroufa K, Liu BH, Teschemacher AG, Kasparov S
  TITLE     Targeting central serotonergic neurons with lentiviral vectors based
            on a transcriptional amplification strategy.
  JOURNAL   Gene Ther. 2009 May;16(5):681-8. Epub 2009 Feb 12.
  PUBMED    19212426
REFERENCE   2  (bases 1 to 12592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 
            Ther."; date: "2009-05"; volume: "16(5)"; pages: "681-8. Epub 2009 
            Feb 12"
COMMENT     Sequence Label: pTYF-G4BS-2TPH-EGFP-IRES-GAL4p65
FEATURES             Location/Qualifiers
     source          1..12592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    81..175
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     regulatory      2241..2250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2247..2249,2250..2252,2253..2963)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2247..2249,2250..2252,2253..2963)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2292..2313)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2553..2572)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2900..2921
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2964..3029
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3087..3660
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3110..3662
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3254..3271)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3481..3500
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3692..4132
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4157..4699
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     primer_bind     complement(4768..4787)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4769..4787)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5061..5182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5098..5117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5152..5171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    5420..5644
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5777..5796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(6817..6834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6988..7576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7068..7087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7747..8538,8539..8607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8370..8389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8608..8712)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8780..8798
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8849..8871)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8957..9095
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9010..9029
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10299..10319
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    11940..12173
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggcctaggga taacagggta atgatatctc tgaccgctag ctcttatcat gtctggatcc
       61 aagcttgcat gcctgcaggt cggagtactg tcctccgagc ggagtactgt cctccgagcg
      121 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
      181 actctagagg gtcccgggat ccgcggactg accactccgt gcctttgctt ttcgtgtctt
      241 ctgcagagag gtcgataccc tttgttctac ttggttatcg cacaacttga ttctctcagc
      301 cagactgtga tctccgtggg tcagtgctgc atcgaacact tctttgcatg cttgggattc
      361 agtttcagct tactagaaac tcagggctca acaaaatttt gttgagttat acatctgttc
      421 tgtgtctggc tctgtagtct gtcgagtctg cttttcttta cacccttctc cttctttgga
      481 agagaacacg gattcccaaa gtcaaattga ctgaattaag atcctacctt agacattctt
      541 ttgtgcctta gtacacacac acacatacac acacacacac acacacacac acagatgcac
      601 acacatacac acatatgcac acacacatac acacagatgc acacacacac atacacatac
      661 acacagatgc acacacgcat acacatacac acagatgcac acacacatac acatacacac
      721 agatgcacac acgcacacac atacacatac acacatgcac acgcacacat aaacatacac
      781 acagatgcac acacgcacac acataaacat acacatgcac acacacacac acatgcacac
      841 acacatgcac atgcacacac acacacacac accagtttta tgataactga ctatgttctg
      901 acctataaac gtgcataaaa atccagaata tgattactta gcattgttag ctattatttt
      961 ttacaattac ttcagttaat ttattaataa tttctcttcc tcttagcctc ttagtcttca
     1021 gaggaaatag gagctaaatg attatttcca gagttggatc aaataataat cctaaaccct
     1081 caagtctcca gaaacagtta aaatccaacc aatgttaaca tatacagact tgttttgaag
     1141 aatcttcatt ttgttttcta tacaatccaa ttgtacgagt cccagatttt gtttagttct
     1201 tcagttaaac atgtactctt ggttaagaat tttccatgtt taaaagttat ttgttatttt
     1261 tacgtacatg agtacactgt agctgtcttc atacacacca gaagagggca tcaaatcccc
     1321 attacatatg attgtgagcc acctagtggt tgctgggatt tgaactcagg acctctggaa
     1381 gagcagtcag cactcttaac cactaagcta tctctccagc cccaaacttt ccattcttta
     1441 aaggattgct aagctttccc gtggctttct aaagttggga aggtacctat agaattttgt
     1501 ctatgcctgt cagattgctg ggtctgatta agttatagat ggggagcagc agaattgcat
     1561 cagaaaagca tcaaagggat agtgggccta tgggcatttc atttccatat gtttcttact
     1621 tagtgaagaa cattctagaa ggtgagcatc ttggctaagt tcaagcctcg aatttcaata
     1681 gcctgagtaa ccttctacct ggagacacca caggttttgg aaacaggcct cttacgaaag
     1741 ccatcacaca tacatacaca taaaatttgc acatcgtaac atgtcatggt gcagaaaagc
     1801 cttccttaag ctattttctt acttttagaa agcacaaagc actcaaactc atagttactg
     1861 gaagactgag ggtaaccttc caatagcagg ccctagaatt aaaattagac gactgctatt
     1921 ttgttgcaag gaaaaaaata ataataaacc ctgacgcata gctctccacc atctcttccc
     1981 aaagggctac tcgacctatg aaacaaacaa atctcatcat gagcacagat aaccccaggc
     2041 ttcagacgtg taatctgatt gtggccatca gcaaccagaa atgagttttt tctaatcagc
     2101 ttcccatcac tcctcagtca ctcatataaa ggaacacagg gaggggagga agcgcactgc
     2161 tcttcagcac cagggttctg gacagcgcct cgagcagcca gctgccgctc accttcctcc
     2221 tacatctctg ccgcggccgc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     2281 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     2341 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     2401 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     2461 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2521 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     2581 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2641 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     2701 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     2761 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2821 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     2881 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2941 tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattctg
     3001 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
     3061 ataccacatt gtaatcccgg gatccgcccc tctccctccc ccccccctaa cgttactggc
     3121 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     3181 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3241 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3301 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3361 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3421 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3481 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3541 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     3601 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     3661 tatggccacc cgcggactag cctcctgaaa gatgaagcta ctgtcttcta tcgaacaagc
     3721 atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa
     3781 gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac
     3841 tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact
     3901 gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa
     3961 agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag
     4021 attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac
     4081 atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccggaatt
     4141 gggatccatg gtgtttcctt ctgggcagat ctcaaaccag gccctggcct tagcaccgtc
     4201 ctctgcccca gtccttgccc agaccatggt cccttcctca gccatggtac ctctggctca
     4261 gcccccagct cctgccccag ttctaacccc gggtcctccc cagtccctgt ctgcacctgt
     4321 tccaaagagc acccaggctg gggaaggcac gctgtcggaa gccctgctgc acctgcagtt
     4381 tgatgctgat gaagacttgg gggccttgct tggcaacagc acagacccag gagtgttcac
     4441 agacctggca tctgtggaca actcagagtt tcagcagctc ctgaaccagg gtgtgtccat
     4501 gtctcactcc acagctgagc ccatgctgat ggagtaccct gaagctataa ctcgcctggt
     4561 gacagggtcc cagaggcccc ctgacccagc tcccacaccc ctggggacct cggggcttcc
     4621 caatggtctc tccggagatg aagacttctc ctccattgcg gacatggact tctctgctct
     4681 gctgagtcag atcagctcca gcggccaatc tagactgcag ctcgacctcg agggggggcc
     4741 cggtaccagg taagtgtacc caattcgccc tatagtgagt cgtattacaa ttcactcgat
     4801 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga gatccaattt ttaagtgtat
     4861 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattcacagt cccaaggctc
     4921 atttcaggcc cctcagtcct cacagtctgt tcatgatcat aatcagccat accacatttg
     4981 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5041 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5101 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5161 ccaaactcat caatgtatct taacgcgcta gtgtacgcgt ctcgaggacg cggccgcatc
     5221 gattagggat aacagggtaa tggtaccttt aagaccaatg acttacaagg cagctgtaga
     5281 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccagggtct
     5341 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5401 aagcctcaat aaagctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5461 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5521 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5581 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5641 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5701 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5761 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5821 tgtgcgggag aacggagttc tattatgact caaatcagtc tccccaagca ttcggggatc
     5881 agagttttta aggataactt agtgtgtagg gggccagtga gttggagatg aaagcgtagg
     5941 gagtcgaagg tgtccttttg cgccgagtca gttcctgggt gggggccaca agatcggatg
     6001 agccagttta tcaatccggg ggtgccagct gatccatgga gtgcagggtc tgcaaaatat
     6061 ctcaagcact gattgatctt aggttttaca atagtgatgt taccccagga acaatttggg
     6121 gaaggtcaga atcttgtagc ctgtagctgc atgactccta aaccataatt tcttttttgt
     6181 tttttttttt ttatttttga gacagggtct cactctgtca cctaggctgg agtgcagtgg
     6241 tgcaatcaca gctcactgca gcctcaacgt cgtaagctca agcgatcctc ccacctcagc
     6301 ctgcctggta gctgagacta caagcgacgc cccagttaat ttttgtattt ttggtagagg
     6361 cagcgttttg ccgtgtggcc ctggctggtc tcgaactcct gggctcaagt gatccagcct
     6421 cagcctccca aagtgctggg acaaccgggg ccagtcactg cacctggccc taaaccataa
     6481 tttctaatct tttggctaat ttgttagtcc tacaaaggca gtctagtccc caggcaaaaa
     6541 gggggtttgt ttcgggaaag ggctgttact gtctttgttt caaactataa actaagttcc
     6601 tcctaaactt agttcggcct acacccagga atgaacaagg agagcttgga ggttagaagc
     6661 acgatggaat tggttaggtc agatctcttt cactgtctga gttataattt tgcaatggtg
     6721 gttcaaagac tgcccgcttc tgacaccagt cgctgcatta atgaatcggc caacgcgcgg
     6781 ggagaggcgg tttgcgtatt ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     7201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8041 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8341 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8701 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     8761 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaactgcc
     8821 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8881 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8941 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtactggc
     9001 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     9061 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     9121 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagcgc ggggaggcag
     9181 agattgcagt aagctgagat cgcagcactg cactccagcc tgggcgacag agtaagactc
     9241 tgtctcaaaa ataaaataaa taaatcaatc agatattcca atcttttcct ttatttattt
     9301 atttattttc tattttggaa acacagtcct tccttattcc agaattacac atatattcta
     9361 tttttcttta tatgctccag ttttttttag accttcacct gaaatgtgtg tatacaaaat
     9421 ctaggccagt ccagcagagc ctaaaggtaa aaaataaaat aataaaaaat aaataaaatc
     9481 tagctcactc cttcacatca aaatggagat acagctgtta gcattaaata ccaaataacc
     9541 catcttgtcc tcaataattt taagcgcctc tctccaccac atctaactcc tgtcaaaggc
     9601 atgtgcccct tccgggcgct ctgctgtgct gccaaccaac tggcatgtgg actctgcagg
     9661 gtccctaact gccaagcccc acagtgtgcc ctgaggctgc cccttccttc tagcggctgc
     9721 ccccactcgg ctttgctttc cctagtttca gttacttgcg ttcagccaag gtctgaaact
     9781 aggtgcgcac agagcggtaa gactgcgaga gaaagagacc agctttacag ggggtttatc
     9841 acagtgcacc ctgacagtcg tcagcctcac agggggttta tcacattgca ccctgacagt
     9901 cgtcagcctc acagggggtt tatcacagtg cacccttaca atcattccat ttgattcaca
     9961 atttttttag tctctactgt gcctaacttg taagttaaat ttgatcagag gtgtgttccc
    10021 agaggggaaa acagtatata cagggttcag tactatcgca tttcagggtg gcccgagagc
    10081 tgcatccgga gtatctagat ggagttccgc gttacataac ttacggtaaa tggcccgcct
    10141 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgactgatgt tcccatagta
    10201 acgccaatag ggactttcca ttgacgctaa tgggagtttg ttttggcacc aaaatcaacg
    10261 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
    10321 actctagaag gtctatataa gcagagctcg tttagtgaac cgtttatact acttatctgg
    10381 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    10441 cttaagcctc aataaagctt gccttgagtg ctcaaagtag tgtgtgcccg tctgttgtgt
    10501 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    10561 ggcgcccgaa cagggacttg aaagcgaaag taaagccaga ggagatctct cgacgcagga
    10621 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
    10681 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc ggtattaagc
    10741 gggggagaat tagataaatg ggaaaaaatt cggttaaggc cagggggaaa gaaacaatat
    10801 aaactaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    10861 cttttagaga catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    10921 acaggatcag aagaacttag atcattatat aatacaatag cagtcctcta ttgtgtgcat
    10981 caaaggatag atgtaaaaga caccaaggaa gccttagata agatagagga agagcaaaac
    11041 aaaagtaaga aaaaggcaca gcaagcagca gctgacacag gaaacaacag ccaggtcagc
    11101 caaaattacc ctatagtgca gaacctccag gggcaaatgg tacatcaggc catatcacct
    11161 agaactttaa atgcatgggt aaaagtagta gaagagaagg ctttcagccc agaagtaata
    11221 cccatgtttt cagcattatc agaaggagcc accccacaag atttaaatac catgctaaac
    11281 acagtggggg gacatcaagc agccatgcaa atgttaaaag agaccatcaa tgaggaagct
    11341 gcagaatggg atagattgca tccagtgcat gcagggccta ttgcaccagg ccagatgaga
    11401 gaaccaaggg gaagtgacat agcaggaact actagcaaat taagagaaca atttggaaat
    11461 aataaaacaa taatctttaa gcaatcctca ggaggggacc cagaaattgt aacgcacagt
    11521 tttaattgtg gaggggaatt tttctactgt aattcaacac aactgtttaa tagtacttgg
    11581 tttaatagta cttggagtac tgaagggtca aataacactg aaggaagtga cacaatcaca
    11641 ctcccatgca gaataaaaca atttataaac atgtggcagg aagtaggaaa agcaatgtat
    11701 gcccctccca tcagtggaca aattagatgt tcatcaaata ttactgggct gctattaaca
    11761 agagatggtg gtaataacaa caatgggtcc gagatcttca gacctggagg aggcgatatg
    11821 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    11881 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    11941 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc acggtcaatg
    12001 acgctgacgg tacaggccag acaattattg tctgatatag tgcagcagca gaacaatttg
    12061 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaaacag
    12121 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    12181 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    12241 aataaatctc tggaacagat ttggaataac atgacctgga tggagtggga cagagaaatt
    12301 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    12361 aatgaacaag aattattgga attagataaa tgggcttgcg gccggaattc acaaatggca
    12421 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    12481 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattactaaa
    12541 aattcnaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg gc
//
