LOCUS       sequence-378232-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ratiometric YDA sensor for in vivo astrocyte-specific cholesterol 
            visualization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Czernecki C, Dixit S, Riezman I, Innocenti S, Bornmann C, Pfrieger 
            FW, Riezman H, Scheiffele P
  TITLE     Cell type-specific assessment of cholesterol distribution in models 
            of neurodevelopmental disorders
  JOURNAL   bioRxiv 2022.11.16.516849
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.11.16.516849"
COMMENT     Sequence Label: pAAV-GfaABC1D-eGFP-2A-mCherry_YDA
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..40
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
       61 acagctcctc gcccttgctc accatggtgg cgaccggtga gctctcccct cacccatttt
      121 taaccggtga gctctcccct cacccatttg tgtccttgga cttctgggaa gcagacagcc
      181 ctcctctccc cccctgccac ccactgtcca tgcctgggca tcccctgatc ccctttcctg
      241 ggcacaggct gaatagagcc ttgttctcca ccgccggtgg cgccacacag caagggcagc
      301 ccctaggggg ctgtgctgct tttatcccaa gatgccaggc tgtcaggctg gggtgcaatg
      361 caagccccct gctcaatggg cttctcggaa ggcatttgag gggggctggg gtgcggcggg
      421 aagcaggcac tgtgcccacc ccgaacattg tgtctgtgcc aaggtgagtc attcactggg
      481 catgaagagg aggccctggg gggcggccag cccaatgctg ccaggtctgc ttgcagacaa
      541 ggcctggctg tctgcctcct ccccagctca cagagccagc tcaggccccc gagcctctgt
      601 cagagcagcg tcccctactc cacaccagga tatgttagat ctacgcgtgc ggccgcagga
      661 acccctagtg atggagttgg ccactcctc
//
