LOCUS       sequence-378234-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ratiometric D4H sensor for in vivo astrocyte-specific cholesterol 
            visualization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Czernecki C, Dixit S, Riezman I, Innocenti S, Bornmann C, Pfrieger 
            FW, Riezman H, Scheiffele P
  TITLE     Cell type-specific assessment of cholesterol distribution in models 
            of neurodevelopmental disorders
  JOURNAL   bioRxiv 2022.11.16.516849
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.11.16.516849"
COMMENT     Sequence Label: pAAV-GfaABC1D-eGFP-2A-mCherry_D4H
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..40
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
       61 acagctcctc gcccttgctc accatggtgg cgaccggtga gctctcccct cacccatttg
      121 tgtccttgga cttctgggaa gcagacagcc ctcctctccc cccctgccac ccactgtcca
      181 tgcctgggca tcccctgatc ccctttcctg ggcacaggct gaatagagcc ttgttctcca
      241 ccgccggtgg cgccacacag caagggcagc ccctaggggg ctgtgctgct tttatcccaa
      301 gatgccaggc tgtcaggctg gggtgcaatg caagccccct gctcaatggg cttctcggaa
      361 ggcatttgag gggggctggg gtgcggcggg aagcaggcac tgtgcccacc ccgaacattg
      421 tgtctgtgcc aaggtgagtc attcactggg catgaagagg aggccctggg gggcggccag
      481 cccaatgctg ccaggtctgc ttgcagacaa ggcctggctg tctgcctcct ccccagctca
      541 cagagccagc tcaggccccc gagcctctgt cagagcagcg tcccctactc cacaccagga
      601 tatgttagat ctacgcgtgc ggccgcagga acccctagtg atggagttgg ccactccctc
      661 tcttgcgcgc t
//
