LOCUS       sequence-378254-       12288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test for if Flp is able to de-repress a circuit with B3RT, to 
            rule out cross-reactivity between these recombinases..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ
FEATURES             Location/Qualifiers
     source          1..12288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      878..1585
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     intron          1685..1873
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2901..3075
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3463..5115
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3529..3547)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5135..5335
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5678..5733
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5741..5761
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6445..6633
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7257..7431
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7501..7522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7537..7542,7543..7560,7561..7567)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7575..7591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7580..7602
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7599..7615
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7599..7615
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7611..7934
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7628..7684
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7685..7702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7685..7701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7694..7716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7910..7929)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8066..8090
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8178..8888
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8909..9013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9014..9808
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9979..10567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10468..10487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10903..12051
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12197..12221
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaaggttgc ttaagaataa gtaattctta agcaaccctg ctttaatgag atatgcgaga
      901 agcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
      961 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     1021 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     1081 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     1141 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     1201 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     1261 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     1321 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     1381 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     1441 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     1501 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     1561 gcgacaacat gtcgaggctc agcagggttg cttaagaata agtaattctt aagcaaccca
     1621 acaaacaaca ttacaattac tatttacaat tacaatgact tcaaaggtgt atgatcctga
     1681 gcaggtaagt ttctgcttct acctttgata tatatataat aattatcatt aattagtagt
     1741 aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt
     1801 tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt
     1861 tgttgatgtg cagagaaaaa gaatgataac tggtccacaa tggtgggcaa gatgtaaaca
     1921 gatgaacgtg cttgattctt tcattaatta ctacgattca gaaaagcacg ctgagaatgc
     1981 agttatcttt cttcacggaa acgctgcatc ttcatatttg tggagacatg ttgtgcctca
     2041 cattgaacca gtggctaggt gtattatccc agatcttatc ggaatgggaa agtctggtaa
     2101 atctggaaat ggttcttaca gacttttgga tcattataag tacctcacag cttggttcga
     2161 acttctcaac ttacctaaga agattatttt cgttggacac gattggggtg cttgcttggc
     2221 attccattac tcatacgaac accaagataa gatcaaagct atagttcatg cagagagtgt
     2281 tgtggatgtg atagaatctt gggatgagtg gccagatatt gaagaggata ttgctttgat
     2341 caagtctgaa gagggagaaa agatggttct cgaaaataac tttttcgtgg aaactatgct
     2401 tccttcaaag attatgagaa aattggaacc agaagagttt gctgcatatc tcgaaccttt
     2461 caaggaaaaa ggagaggtta gaaggccaac actcagttgg cctagggaaa taccattagt
     2521 taagggaggt aaacctgatg ttgtgcagat cgtgagaaat tataacgctt accttagggc
     2581 atcagatgat ttgcctaaga tgttcatcga gtcagatcca ggatttttca gtaatgctat
     2641 agttgaaggt gcaaagaaat ttccaaacac cgagttcgtt aaggtgaaag gattgcattt
     2701 ctcacaagaa gatgctcctg atgagatggg aaagtatatt aagtcttttg ttgaaagagt
     2761 gctcaaaaac gagcagggtt ctcgatctga tgcttctgaa gtaacgtcaa caagctccca
     2821 gtctgaggta tgcacagtgg aaacgccagg ttgtgttggt tcttaagctt ctctagctag
     2881 agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
     2941 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     3001 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     3061 actaaaatcc agatcgctgc aagaattcaa gcttggagcc aacactcgaa tccccacccg
     3121 ttggaccaaa cccggctcat taagcgtcgg ttcagattta tttcctttat ttaaaaaaaa
     3181 ggaaagggta aaaaatagaa aattggaaac agttaaagcc caaaattgta atttaccgag
     3241 aattgtaaat ttacctgaaa accctacgct atagtttcga ctataaatac caaacttagg
     3301 acctcacttc agaatcccct cgtcgctgcg tctctctccc gcaaccttcg attttcgttt
     3361 attcgcatcc atcggagaga gaaaacaatc aataagcgac ctactacaaa caagtaagta
     3421 agagaaaaac caaaagaaga agagaaacaa caagaagaag taatggagga cgccaaaaac
     3481 ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg agagcaactg
     3541 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
     3601 atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct
     3661 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
     3721 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
     3781 gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc taccgtagtg
     3841 tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc
     3901 cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     3961 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc agagtccttt
     4021 gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg gttacctaag
     4081 ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag agatcctatt
     4141 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     4201 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     4261 agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca aagtgcgttg
     4321 ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa atacgattta
     4381 tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt cggggaagcg
     4441 gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac tgagactaca
     4501 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     4561 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcag
     4621 agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa caatccggaa
     4681 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     4741 gacgaggacg aacacttctt catagttgac cgcttgaagt ctttaattaa atacaaagga
     4801 tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa catcttcgac
     4861 gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     4921 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     4981 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     5041 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     5101 aagtccaaat tgtaagcttg atcccccgtc gacagctagc tatatcatca atttatgtat
     5161 tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga tataatcagt
     5221 tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg cttggactat
     5281 aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc aagatcgcta
     5341 ctagaattcg agctcggagc caacactcga atccccaccc gttggaccaa acccggctca
     5401 ttaagcgtcg gttcagattt atttccttta tttaaaaaaa aggaaagggt aaaaaataga
     5461 aaattggaaa cagttaaagc ccaaaattgt aatttaccga gaattgtaaa tttacctgaa
     5521 aaccctacgc tatagtttcg actataaata ccaaacttag gacctcactt cagaatcccc
     5581 tcgtcgctgc gtctctctcc cgcaaccttc gattttcgtt tattcgcatc catcggagag
     5641 agaaaacaat caataagcga cctactgtat ttttacaaca attaccaaca acaacaaaca
     5701 acaaacaaca ttacaattac tatttacaat tacaatggct cctaagaaga agaggaaggt
     5761 gatgagccag ttcgacatcc tgtgcaagac cccccccaag gtgctggtgc ggcagttcgt
     5821 ggagagattc gagaggccca gcggcgagaa gatcgccagc tgtgccgccg agctgaccta
     5881 cctgtgctgg atgatcaccc acaacggcac cgccatcaag agggccacct tcatgagcta
     5941 caacaccatc atcagcaaca gcctgagctt cgacatcgtg aacaagagcc tgcagttcaa
     6001 gtacaagacc cagaaggcca ccatcctgga ggccagcctg aagaagctga tccccgcctg
     6061 ggagttcacc atcatccctt acaacggcca gaagcaccag agcgacatca ccgacatcgt
     6121 gtccagcctg cagctgcagt tcgagagcag cgaggaggcc gacaagggca acagccacag
     6181 caagaagatg ctgaaggccc tgctgtccga gggcgagagc atctgggaga tcaccgagaa
     6241 gatcctgaac agcttcgagt acaccagcag gttcaccaag accaagaccc tgtaccagtt
     6301 cctgttcctg gccacattca tcaactgcgg caggttcagc gacatcaaga acgtggaccc
     6361 caagagcttc aagctggtgc agaacaagta cctgggcgtg atcattcagt gcctggtgac
     6421 cgagactaag acaagcgtgt ccaggtaagt ttctgcttct acctttgata tatatataat
     6481 aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg
     6541 tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt
     6601 tctaatatat gaccaaaatt tgttgatgtg caggcacatc tactttttca gcgccagagg
     6661 caggatcgac cccctggtgt acctggacga gttcctgagg aacagcgagc ccgtgctgaa
     6721 gagagtgaac aggaccggca acagcagcag caacaagcag gagtaccagc tgctgaagga
     6781 caacctggtg cgcagctaca acaaggccct gaagaagaac gccccctacc ccatcttcgc
     6841 tatcaagaac ggccctaaga gccacatcgg caggcacctg atgaccagct ttctgagcat
     6901 gaagggcctg accgagctga caaacgtggt gggcaactgg agcgacaaga gggcctccgc
     6961 cgtggccagg accacctaca cccaccagat caccgccatc cccgaccact acttcgccct
     7021 ggtgtccagg tactacgcct acgaccccat cagcaaggag atgatcgccc tgaaggacga
     7081 gactaacccc atcgaggagt ggcagcacat cgagcagctg aagggcagcg ccgagggcag
     7141 catcagatac cccgcctgga acggcatcat cagccaggag gtgctggact acctgagcag
     7201 ctacatcaac aggcggatct gagcttctct agctagagtc gatcgacaag ctcgagtttc
     7261 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     7321 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     7381 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctttactg
     7441 agaccgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7501 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7561 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7621 ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
     7681 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7741 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7801 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     7861 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     7921 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7981 gggcttgtct gctcccggca tccgcttaca gacaagctgt gacggtctca gggacacgaa
     8041 gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     8101 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     8161 catttgtatg tgccagccgc ctttgcgacg ctcaccgggc tggttgccct cgccgctggg
     8221 ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga
     8281 caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     8341 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     8401 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     8461 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     8521 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     8581 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     8641 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     8701 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     8761 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
     8821 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
     8881 aatggcagcg ctggccaatt cccgagtgcg cggaacccct atttgtttat ttttctaaat
     8941 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9001 aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     9061 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     9121 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     9181 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     9241 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     9301 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     9361 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     9421 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     9481 cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     9541 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     9601 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     9661 caagtggtat gacattgcct tctgcgtccg gtcgctcagg gaggatatcg gggaagaaca
     9721 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     9781 ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat atatacttta
     9841 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9901 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9961 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10021 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10081 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10141 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10201 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10261 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10321 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10381 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10441 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10501 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10561 atggaaaaac gccagcaacg cggccttttt acggttcctg gcagatccta gatgtggcgc
    10621 aacgatgccg gcgacaagca ggagcgcacc gacttcttcc gcatcaagtg ttttggctct
    10681 caggccgagg cccacggcaa gtatttgggc aaggggtcgc tggtattcgt gcagggcaag
    10741 attcggaata ccaagtacga gaaggacggc cagacggtct acgggaccga cttcattgcc
    10801 gataaggtgg attatctgga caccaaggca ccaggcgggt caaatcagga ataagggcac
    10861 attgccccgg cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg gacgtttgac
    10921 cggaaggcat acaggcaaga actgatcgac gcggggtttt ccgccgagga tgccgaaacc
    10981 atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt cggctcgatg
    11041 gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc aactggctcc ccctgccctg
    11101 cccgcgccat cggccgccgt ggagcgttcg cgtcgtcttg aacaggaggc ggcaggtttg
    11161 gcgaagtcga tgaccatcga cacgcgagga actatgacga ccaagaagcg aaaaaccgcc
    11221 ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct gaaacacacg
    11281 aagcagcaga tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg gccggacacg
    11341 atgcgagcga tgccaaacga cacggcccgc tctgccctgt tcaccacgcg caacaagaaa
    11401 atcccgcgcg aggcgctgca aaacaaggtc attttccacg tcaacaagga cgtgaagatc
    11461 acctacaccg gcgtcgagct gcgggccgac gatgacgaac tggtgtggca gcaggtgttg
    11521 gagtacgcga agcgcacccc tatcggcgag ccgatcacct tcacgttcta cgagctttgc
    11581 caggacctgg gctggtcgat caatggccgg tattacacga aggccgagga atgcctgtcg
    11641 cgcctacagg cgacggcgat gggcttcacg tccgaccgcg ttgggcacct ggaatcggtg
    11701 tcgctgctgc accgcttccg cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtc
    11761 ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc actacacgaa attcatatgg
    11821 gagaagtacc gcaagctgtc gccgacggcc cgacggatgt tcgactattt cagctcgcac
    11881 cgggagccgt acccgctcaa gctggaaacc ttccgcctca tgtgcggatc ggattccacc
    11941 cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg aggcagcggc
    12001 ctggtggaac acgcctgggt caatgatgac ctggtgcatt gcaaacgcta gggccttgtg
    12061 gggtcagttc cggctggggg ttcagcagcc cctgctcgga tctgttggac cggacagtag
    12121 tcatggttga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    12181 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    12241 ttaataacac attgcggacg tttttaatgt actggggttg aacactct
//
