LOCUS       sequence-378279-        9777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test for the repressive potential of the OCS and Transcription 
            Block (TB) fusion with the FRT sites around it..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC
FEATURES             Location/Qualifiers
     source          1..9777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     polyA_signal    1587..1635
                     /note="synthetic polyadenylation signal"
     misc_feature    1649..1740
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1689..1708
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    1741..1774
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1841..2029
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    3057..3231
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3619..5271
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3685..3703)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5291..5491
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5555..5579
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5667..6377
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6398..6502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6503..7297
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7468..8056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7957..7976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8392..9540
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9686..9710
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcagaata aaatatcttt attttcatta catctgtgtg
     1621 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     1681 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     1741 gaagttccta ttctctagaa agtataggaa cttccaacaa acaacattac aattactatt
     1801 tacaattaca atgacttcaa aggtgtatga tcctgagcag gtaagtttct gcttctacct
     1861 ttgatatata tataataatt atcattaatt agtagtaata taatatttca aatatttttt
     1921 tcaaaataaa agaatgtagt atatagcaat tgcttttctg tagtttataa gtgtgtatat
     1981 tttaatttat aacttttcta atatatgacc aaaatttgtt gatgtgcaga gaaaaagaat
     2041 gataactggt ccacaatggt gggcaagatg taaacagatg aacgtgcttg attctttcat
     2101 taattactac gattcagaaa agcacgctga gaatgcagtt atctttcttc acggaaacgc
     2161 tgcatcttca tatttgtgga gacatgttgt gcctcacatt gaaccagtgg ctaggtgtat
     2221 tatcccagat cttatcggaa tgggaaagtc tggtaaatct ggaaatggtt cttacagact
     2281 tttggatcat tataagtacc tcacagcttg gttcgaactt ctcaacttac ctaagaagat
     2341 tattttcgtt ggacacgatt ggggtgcttg cttggcattc cattactcat acgaacacca
     2401 agataagatc aaagctatag ttcatgcaga gagtgttgtg gatgtgatag aatcttggga
     2461 tgagtggcca gatattgaag aggatattgc tttgatcaag tctgaagagg gagaaaagat
     2521 ggttctcgaa aataactttt tcgtggaaac tatgcttcct tcaaagatta tgagaaaatt
     2581 ggaaccagaa gagtttgctg catatctcga acctttcaag gaaaaaggag aggttagaag
     2641 gccaacactc agttggccta gggaaatacc attagttaag ggaggtaaac ctgatgttgt
     2701 gcagatcgtg agaaattata acgcttacct tagggcatca gatgatttgc ctaagatgtt
     2761 catcgagtca gatccaggat ttttcagtaa tgctatagtt gaaggtgcaa agaaatttcc
     2821 aaacaccgag ttcgttaagg tgaaaggatt gcatttctca caagaagatg ctcctgatga
     2881 gatgggaaag tatattaagt cttttgttga aagagtgctc aaaaacgagc agggttctcg
     2941 atctgatgct tctgaagtaa cgtcaacaag ctcccagtct gaggtatgca cagtggaaac
     3001 gccaggttgt gttggttctt aagcttctct agctagagtc gatcgacaag ctcgagtttc
     3061 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     3121 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     3181 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctgcaaga
     3241 attcaagctt ggagccaaca ctcgaatccc cacccgttgg accaaacccg gctcattaag
     3301 cgtcggttca gatttatttc ctttatttaa aaaaaaggaa agggtaaaaa atagaaaatt
     3361 ggaaacagtt aaagcccaaa attgtaattt accgagaatt gtaaatttac ctgaaaaccc
     3421 tacgctatag tttcgactat aaataccaaa cttaggacct cacttcagaa tcccctcgtc
     3481 gctgcgtctc tctcccgcaa ccttcgattt tcgtttattc gcatccatcg gagagagaaa
     3541 acaatcaata agcgacctac tacaaacaag taagtaagag aaaaaccaaa agaagaagag
     3601 aaacaacaag aagaagtaat ggaggacgcc aaaaacataa agaaaggccc ggcgccattc
     3661 tatcctctag aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
     3721 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg
     3781 gaatacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
     3841 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
     3901 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
     3961 ctcaacagta tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa
     4021 aaaattttga acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct
     4081 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
     4141 ggttttaatg aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg
     4201 ataatgaatt cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact
     4261 gcctgcgtca gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
     4321 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
     4381 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta
     4441 cgatcccttc aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc
     4501 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
     4561 gggggcgcac ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca
     4621 gggatacgac aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     4681 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     4741 gatctggata ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga
     4801 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     4861 gatggatggc tacattctgg agacatagct tactgggacg aggacgaaca cttcttcata
     4921 gttgaccgct tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg
     4981 gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tctgcccgac
     5041 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     5101 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     5161 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     5221 atcagagaga tcctcataaa ggccaagaag ggcggaaagt ccaaattgta agcttgatcc
     5281 cccgtcgaca gctagctata tcatcaattt atgtattaca cataatatcg cactcagtct
     5341 ttcatctacg gcaatgtacc agctgatata atcagttatt gaaatatttc tgaatttaaa
     5401 cttgcatcaa taaatttatg tttttgcttg gactataata cctgacttgt tattttatca
     5461 ataaatattt aaactatatt tctttcaaga tcgctactag aggatgcaca tgtgaccgag
     5521 ggacacgaag tgatccgttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     5581 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     5641 ccgttcgtcc atttgtatgt gccagccgcc tttgcgacgc tcaccgggct ggttgccctc
     5701 gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg
     5761 tgtgcgagac accgcggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact
     5821 gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca
     5881 gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc
     5941 gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt
     6001 gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg
     6061 acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg
     6121 tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa
     6181 cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc
     6241 gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg
     6301 gcccgctaac gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct
     6361 caccccaaaa atggcagcgc tggccaattc ccgagtgcgc ggaaccccta tttgtttatt
     6421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6481 ataatattga aaaaggaaga gtatggctaa aatgagaata tcaccggaat tgaaaaaact
     6541 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     6601 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     6661 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     6721 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     6781 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     6841 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     6901 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     6961 tgtggattgc gaaaactggg aagaggacac tccatttaaa gatccgcgcg agctgtatga
     7021 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     7081 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     7141 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgctcaggg aggatatcgg
     7201 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     7261 aataaaatat tatattttac tggatgaatt gttttagctg tcagaccaag tttactcata
     7321 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7381 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7441 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7501 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7561 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7621 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7681 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7741 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7801 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7861 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7921 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7981 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8041 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg cagatcctag
     8101 atgtggcgca acgatgccgg cgacaagcag gagcgcaccg acttcttccg catcaagtgt
     8161 tttggctctc aggccgaggc ccacggcaag tatttgggca aggggtcgct ggtattcgtg
     8221 cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac
     8281 ttcattgccg ataaggtgga ttatctggac accaaggcac caggcgggtc aaatcaggaa
     8341 taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg
     8401 acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat
     8461 gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc
     8521 ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc
     8581 cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtcttga acaggaggcg
     8641 gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga
     8701 aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg
     8761 aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg
     8821 ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc
     8881 aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac
     8941 gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag
     9001 caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac
     9061 gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa
     9121 tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg
     9181 gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt
     9241 tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa
     9301 ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc
     9361 agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg
     9421 gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga
     9481 ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag
     9541 ggccttgtgg ggtcagttcc ggctgggggt tcagcagccc ctgctcggat ctgttggacc
     9601 ggacagtagt catggttgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     9661 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     9721 ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggttga acactct
//
