LOCUS       sequence-378283-       12098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the on state for testing of the alt Vika recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12098)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ
FEATURES             Location/Qualifiers
     source          1..12098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5680..5735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5740..5760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6460..6648
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7067..7241
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7311..7332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7347..7352,7353..7370,7371..7377)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7385..7401
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7390..7412
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7409..7425
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7409..7425
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7421..7744
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7438..7494
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7495..7512)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7495..7511)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7504..7526)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7720..7739)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    7876..7900
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7988..8698
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8719..8823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8824..9618
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9789..10377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10278..10297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10713..11861
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12007..12031
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaaaatagg tctgagaacg cccattctca gacgtattct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcagaata ggtctgagaa cgcccattct cagacgtatt
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccgaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct
     5401 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
     5461 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
     5521 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
     5581 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
     5641 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
     5701 caacaaacaa cattacaatt actatttaca attacaatgc ccaagaaaaa gcggaaagtg
     5761 accgacctga ccccattccc ccccctggaa cacctggaac ccgacgagtt tgccgacctc
     5821 gtgcggaagg ccatcaagag ggatcctcag gctggcgccc accctgccat ccagtctgcc
     5881 atcagccact tccaggacga gttcgtgcgg agacagggcg aatggcagcc tgccacactg
     5941 cagagactga gaaacgcctg gaatgtgttt gtgcggtggt gcacccacca gggcattcca
     6001 gctctgcctg ccagacacca ggacgtggaa agatacctga tcgagcggcg gaacgagctg
     6061 caccggaaca ccctgaaagt gcacctgtgg gccatcggca agacccacgt gatcagcggc
     6121 ctgcccaatc cctgcgccca cagatacgtg aaagcccaga tggcccagat cacacaccag
     6181 aaagtgcgcg agagagagcg gatcgaacag gcccctgcct tcagagagtc cgacctggac
     6241 agactgaccg agctgtggag cgccaccaga agcgtgaccc agcagcggga cctgatgatc
     6301 gtgtccctgg cctacgagac actgctgcgg aagaacaatc tggaacagat gaaagtgggc
     6361 gacatcgagt tctgccagga cggctctgcc ctgatcacca tccccttcag caagaccaac
     6421 cacagcggca gggatgacgt gcggtggatc tctccccagg taagtttctg cttctacctt
     6481 tgatatatat ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt
     6541 caaaataaaa gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt
     6601 ttaatttata acttttctaa tatatgacca aaatttgttg atgtgcaggt ggccaatcag
     6661 gtgcacgcct acctgcagct gcccaacatc gacgccgacc cccagtgctt cctgctgcag
     6721 agagtgaaga gaagcggcaa ggccctgaac cccgagagcc acaataccct gaacggccac
     6781 caccccgtgt ccgagaagct gatctcccgg gtgttcgagc gggcttggag agccctgaat
     6841 cacgagacag gccccagata caccggccac agcgctagag tgggagccgc tcaggatctg
     6901 ctgcaggaag gctacagcac cctgcaagtg atgcaggcag gcggctggtc cagcgagaag
     6961 atggtgctga gatacggccg gcatctgcac gcccacacat ctgccatggc tcagaaacgg
     7021 cggcagcggt gagcttctct agctagagtc gatcgacaag ctcgagtttc tccataataa
     7081 tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg
     7141 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     7201 tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctttactg agaccgcagc
     7261 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7321 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7381 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7441 cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga attcactggc
     7501 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     7561 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7621 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     7681 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     7741 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7801 gctcccggca tccgcttaca gacaagctgt gacggtctca gggacacgaa gtgatccgtt
     7861 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     7921 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     7981 tgccagccgc ctttgcgacg ctcaccgggc tggttgccct cgccgctggg ctggcggccg
     8041 tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc
     8101 gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg
     8161 ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat
     8221 ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg
     8281 cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact
     8341 tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg
     8401 ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca
     8461 gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt
     8521 ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc
     8581 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcgggcctc
     8641 ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg
     8701 ctggccaatt cccgagtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8761 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8821 agtatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
     8881 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
     8941 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
     9001 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
     9061 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
     9121 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
     9181 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
     9241 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
     9301 gaagaggaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
     9361 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
     9421 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
     9481 gacattgcct tctgcgtccg gtcgctcagg gaggatatcg gggaagaaca gtatgtcgag
     9541 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
     9601 ctggatgaat tgttttagct gtcagaccaa gtttactcat atatacttta gattgattta
     9661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9781 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9841 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9901 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9961 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10021 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10081 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10141 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10201 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10261 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10321 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10381 gccagcaacg cggccttttt acggttcctg gcagatccta gatgtggcgc aacgatgccg
    10441 gcgacaagca ggagcgcacc gacttcttcc gcatcaagtg ttttggctct caggccgagg
    10501 cccacggcaa gtatttgggc aaggggtcgc tggtattcgt gcagggcaag attcggaata
    10561 ccaagtacga gaaggacggc cagacggtct acgggaccga cttcattgcc gataaggtgg
    10621 attatctgga caccaaggca ccaggcgggt caaatcagga ataagggcac attgccccgg
    10681 cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg gacgtttgac cggaaggcat
    10741 acaggcaaga actgatcgac gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc
    10801 gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt cggctcgatg gtccagcaag
    10861 ctacggccaa gatcgagcgc gacagcgtgc aactggctcc ccctgccctg cccgcgccat
    10921 cggccgccgt ggagcgttcg cgtcgtcttg aacaggaggc ggcaggtttg gcgaagtcga
    10981 tgaccatcga cacgcgagga actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc
    11041 tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct gaaacacacg aagcagcaga
    11101 tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg gccggacacg atgcgagcga
    11161 tgccaaacga cacggcccgc tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg
    11221 aggcgctgca aaacaaggtc attttccacg tcaacaagga cgtgaagatc acctacaccg
    11281 gcgtcgagct gcgggccgac gatgacgaac tggtgtggca gcaggtgttg gagtacgcga
    11341 agcgcacccc tatcggcgag ccgatcacct tcacgttcta cgagctttgc caggacctgg
    11401 gctggtcgat caatggccgg tattacacga aggccgagga atgcctgtcg cgcctacagg
    11461 cgacggcgat gggcttcacg tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc
    11521 accgcttccg cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg
    11581 aggaaatcgt cgtgctgttt gctggcgacc actacacgaa attcatatgg gagaagtacc
    11641 gcaagctgtc gccgacggcc cgacggatgt tcgactattt cagctcgcac cgggagccgt
    11701 acccgctcaa gctggaaacc ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga
    11761 agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg aggcagcggc ctggtggaac
    11821 acgcctgggt caatgatgac ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc
    11881 cggctggggg ttcagcagcc cctgctcgga tctgttggac cggacagtag tcatggttga
    11941 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
    12001 ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac
    12061 attgcggacg tttttaatgt actggggttg aacactct
//
