LOCUS       sequence-378286-       15618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 2-input state of the AND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15618)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 15618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ
FEATURES             Location/Qualifiers
     source          1..15618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      878..1585
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1649..1682
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      1683..2390
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    2391..2424
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          2491..2679
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    3707..3881
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             4269..5921
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4335..4353)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5941..6141
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    6484..6539
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6544..6564
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7342..7530
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    8492..8666
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9008..9063
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             9071..9091
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9775..9963
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    10587..10761
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    10831..10852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10867..10872,10873..10890,10891..10897)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10905..10921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10910..10932
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10929..10945
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10929..10945
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             10941..11264
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    10958..11014
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(11015..11032)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11015..11031)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11024..11046)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(11240..11259)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    11396..11420
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      11508..12218
                     /label=oriV
                     /note="incP origin of replication"
     promoter        12239..12343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12344..13138
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13309..13897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13798..13817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             14233..15381
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    15527..15551
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaaggttgc ttaagaataa gtaattctta agcaaccctg ctttaatgag atatgcgaga
      901 agcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
      961 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     1021 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     1081 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     1141 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     1201 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     1261 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     1321 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     1381 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     1441 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     1501 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     1561 gcgacaacat gtcgaggctc agcagggttg cttaagaata agtaattctt aagcaacctc
     1621 tgtagagtga acttcaccac atattctgga agttcctatt ctctagaaag tataggaact
     1681 tcctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct ttcaattctg
     1741 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
     1801 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
     1861 aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg
     1921 tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt
     1981 ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga
     2041 ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg
     2101 agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt
     2161 gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca
     2221 acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt
     2281 ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc
     2341 gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag gaagttccta
     2401 ttctctagaa agtataggaa cttccaacaa acaacattac aattactatt tacaattaca
     2461 atgacttcaa aggtgtatga tcctgagcag gtaagtttct gcttctacct ttgatatata
     2521 tataataatt atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa
     2581 agaatgtagt atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat
     2641 aacttttcta atatatgacc aaaatttgtt gatgtgcaga gaaaaagaat gataactggt
     2701 ccacaatggt gggcaagatg taaacagatg aacgtgcttg attctttcat taattactac
     2761 gattcagaaa agcacgctga gaatgcagtt atctttcttc acggaaacgc tgcatcttca
     2821 tatttgtgga gacatgttgt gcctcacatt gaaccagtgg ctaggtgtat tatcccagat
     2881 cttatcggaa tgggaaagtc tggtaaatct ggaaatggtt cttacagact tttggatcat
     2941 tataagtacc tcacagcttg gttcgaactt ctcaacttac ctaagaagat tattttcgtt
     3001 ggacacgatt ggggtgcttg cttggcattc cattactcat acgaacacca agataagatc
     3061 aaagctatag ttcatgcaga gagtgttgtg gatgtgatag aatcttggga tgagtggcca
     3121 gatattgaag aggatattgc tttgatcaag tctgaagagg gagaaaagat ggttctcgaa
     3181 aataactttt tcgtggaaac tatgcttcct tcaaagatta tgagaaaatt ggaaccagaa
     3241 gagtttgctg catatctcga acctttcaag gaaaaaggag aggttagaag gccaacactc
     3301 agttggccta gggaaatacc attagttaag ggaggtaaac ctgatgttgt gcagatcgtg
     3361 agaaattata acgcttacct tagggcatca gatgatttgc ctaagatgtt catcgagtca
     3421 gatccaggat ttttcagtaa tgctatagtt gaaggtgcaa agaaatttcc aaacaccgag
     3481 ttcgttaagg tgaaaggatt gcatttctca caagaagatg ctcctgatga gatgggaaag
     3541 tatattaagt cttttgttga aagagtgctc aaaaacgagc agggttctcg atctgatgct
     3601 tctgaagtaa cgtcaacaag ctcccagtct gaggtatgca cagtggaaac gccaggttgt
     3661 gttggttctt aagcttctct agctagagtc gatcgacaag ctcgagtttc tccataataa
     3721 tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg
     3781 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     3841 tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctgcaaga attcaagctt
     3901 ggagccaaca ctcgaatccc cacccgttgg accaaacccg gctcattaag cgtcggttca
     3961 gatttatttc ctttatttaa aaaaaaggaa agggtaaaaa atagaaaatt ggaaacagtt
     4021 aaagcccaaa attgtaattt accgagaatt gtaaatttac ctgaaaaccc tacgctatag
     4081 tttcgactat aaataccaaa cttaggacct cacttcagaa tcccctcgtc gctgcgtctc
     4141 tctcccgcaa ccttcgattt tcgtttattc gcatccatcg gagagagaaa acaatcaata
     4201 agcgacctac tacaaacaag taagtaagag aaaaaccaaa agaagaagag aaacaacaag
     4261 aagaagtaat ggaggacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag
     4321 aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     4381 gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg
     4441 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     4501 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     4561 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     4621 tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga
     4681 acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct aaaacggatt
     4741 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     4801 aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt
     4861 cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca
     4921 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     4981 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     5041 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc
     5101 aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa
     5161 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac
     5221 ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac
     5281 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     5341 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     5401 ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta
     5461 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     5521 tacattctgg agacatagct tactgggacg aggacgaaca cttcttcata gttgaccgct
     5581 tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat
     5641 tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tctgcccgac gatgacgccg
     5701 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     5761 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     5821 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     5881 tcctcataaa ggccaagaag ggcggaaagt ccaaattgta agcttgatcc cccgtcgaca
     5941 gctagctata tcatcaattt atgtattaca cataatatcg cactcagtct ttcatctacg
     6001 gcaatgtacc agctgatata atcagttatt gaaatatttc tgaatttaaa cttgcatcaa
     6061 taaatttatg tttttgcttg gactataata cctgacttgt tattttatca ataaatattt
     6121 aaactatatt tctttcaaga tcgctactag aattcgagct cggagccaac actcgaatcc
     6181 ccacccgttg gaccaaaccc ggctcattaa gcgtcggttc agatttattt cctttattta
     6241 aaaaaaagga aagggtaaaa aatagaaaat tggaaacagt taaagcccaa aattgtaatt
     6301 taccgagaat tgtaaattta cctgaaaacc ctacgctata gtttcgacta taaataccaa
     6361 acttaggacc tcacttcaga atcccctcgt cgctgcgtct ctctcccgca accttcgatt
     6421 ttcgtttatt cgcatccatc ggagagagaa aacaatcaat aagcgaccta ctgtattttt
     6481 acaacaatta ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca
     6541 atgcccaaga aaaagcggaa ggtgtccagc tacatggacc tggtggacga cgagcccgcc
     6601 accctgtacc acaagttcgt ggaatgcctg aaggccggcg agaacttctg cggcgataag
     6661 ctgagcggca tcatcaccat ggccattctg aaggccatca aggccctgac cgaagtgaag
     6721 aaaaccacct tcaacaagta caagaccacc atcaagcagg gcctgcagta cgacgtgggc
     6781 agcagcacca tcagcttcgt gtaccacctg aaggactgcg acgagctgag cagaggcctg
     6841 agcgacgcct tcgagcccta caagttcaag atcaagagca acaaagaggc caccagcttc
     6901 aagaccctgt tcaggggccc tagcttcggc agccagaaga actggcggaa gaaagaggtg
     6961 gaccgcgagg tggacaacct gttccacagc accgagacag acgagagcat cttcaagttc
     7021 atcctgaaca ccctggacag catcgaaacc cagaccaaca ccgaccggca gaaaaccgtg
     7081 ctgaccttta tcctgctgat gaccttcttc aactgctgcc ggaacaacga cctgatgaac
     7141 gtggacccca gcaccttcaa gatcgtgaag aacaagtttg tgggctacct gctgcaggct
     7201 gaagtgaagc agaccaagac cagaaagagc cggaatatct tcttcttccc catccgggaa
     7261 aaccgcttcg acctgttcct ggccctgcac gacttcttca gaacctgcca gcccaccccc
     7321 aagagcagac tgagcgatca ggtaagtttc tgcttctacc tttgatatat atataataat
     7381 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag
     7441 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct
     7501 aatatatgac caaaatttgt tgatgtgcag gtgtccgagc agaagtggca gctgttccgg
     7561 gacagcatgg tcatcgacta caaccggttc tttcggaagt tccccgccag ccccatcttc
     7621 gccattaagc acggccccaa gtcccacctg ggccggcatc tgatgaacag ctttctgcac
     7681 aagaacgagc tggacagctg ggccaacagc ctgggcaatt ggagcagctc ccagaaccag
     7741 agagagagcg gcgccagact gggctacaca cacggcggaa gagatctgcc ccagcccctg
     7801 tttggcttcc tggccggata ctgcgtgcgg aacgaagagg gccacatcgt gggcctgggc
     7861 ctggaaaagg acatcaacga tctgttcgac ggcatcatgg accccctgaa cgagaaagag
     7921 gacaccgaga tctgcgagag ctacggcgag tgggccaaga ttgtgtccaa ggacgtgctg
     7981 atcttcctga agagatacca cagcaagaac gcctgtcgga gataccagaa cagcaccctg
     8041 tatgcccgga ccttcctgaa aaccgagagc gtgaccctga gcggctccaa gggcagcgag
     8101 gaaccttcta gccctgtgcg gatccccatc ctgagcatgg gaaaggccag cccctccgag
     8161 ggaagaaagc tgagagccag cgagcacgcc aacgacgaca acgagatcga gaagatcgac
     8221 agcgacagca gccagagcga agagatccct atcgagatga gcgactccga ggacgagaca
     8281 accgccagca acatcagcgg catctacctg gacatgagca aggccaactc caacgtggtg
     8341 tacagccccc ctagccagac aggcagagct gctggcgccg gaagaaaaag aggcgtggga
     8401 ggcagacgga ccgtggaaag caagcggaga agagtgctgg cccccatcaa ccggtgagct
     8461 tctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
     8521 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
     8581 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     8641 caaaatccag tactaaaatc cagatcgctt tacgaattcc catggggagc caacactcga
     8701 atccccaccc gttggaccaa acccggctca ttaagcgtcg gttcagattt atttccttta
     8761 tttaaaaaaa aggaaagggt aaaaaataga aaattggaaa cagttaaagc ccaaaattgt
     8821 aatttaccga gaattgtaaa tttacctgaa aaccctacgc tatagtttcg actataaata
     8881 ccaaacttag gacctcactt cagaatcccc tcgtcgctgc gtctctctcc cgcaaccttc
     8941 gattttcgtt tattcgcatc catcggagag agaaaacaat caataagcga cctactgtat
     9001 ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac tatttacaat
     9061 tacaatggct cctaagaaga agaggaaggt gatgagccag ttcgacatcc tgtgcaagac
     9121 cccccccaag gtgctggtgc ggcagttcgt ggagagattc gagaggccca gcggcgagaa
     9181 gatcgccagc tgtgccgccg agctgaccta cctgtgctgg atgatcaccc acaacggcac
     9241 cgccatcaag agggccacct tcatgagcta caacaccatc atcagcaaca gcctgagctt
     9301 cgacatcgtg aacaagagcc tgcagttcaa gtacaagacc cagaaggcca ccatcctgga
     9361 ggccagcctg aagaagctga tccccgcctg ggagttcacc atcatccctt acaacggcca
     9421 gaagcaccag agcgacatca ccgacatcgt gtccagcctg cagctgcagt tcgagagcag
     9481 cgaggaggcc gacaagggca acagccacag caagaagatg ctgaaggccc tgctgtccga
     9541 gggcgagagc atctgggaga tcaccgagaa gatcctgaac agcttcgagt acaccagcag
     9601 gttcaccaag accaagaccc tgtaccagtt cctgttcctg gccacattca tcaactgcgg
     9661 caggttcagc gacatcaaga acgtggaccc caagagcttc aagctggtgc agaacaagta
     9721 cctgggcgtg atcattcagt gcctggtgac cgagactaag acaagcgtgt ccaggtaagt
     9781 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat
     9841 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt
     9901 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg
     9961 caggcacatc tactttttca gcgccagagg caggatcgac cccctggtgt acctggacga
    10021 gttcctgagg aacagcgagc ccgtgctgaa gagagtgaac aggaccggca acagcagcag
    10081 caacaagcag gagtaccagc tgctgaagga caacctggtg cgcagctaca acaaggccct
    10141 gaagaagaac gccccctacc ccatcttcgc tatcaagaac ggccctaaga gccacatcgg
    10201 caggcacctg atgaccagct ttctgagcat gaagggcctg accgagctga caaacgtggt
    10261 gggcaactgg agcgacaaga gggcctccgc cgtggccagg accacctaca cccaccagat
    10321 caccgccatc cccgaccact acttcgccct ggtgtccagg tactacgcct acgaccccat
    10381 cagcaaggag atgatcgccc tgaaggacga gactaacccc atcgaggagt ggcagcacat
    10441 cgagcagctg aagggcagcg ccgagggcag catcagatac cccgcctgga acggcatcat
    10501 cagccaggag gtgctggact acctgagcag ctacatcaac aggcggatct gagcttctct
    10561 agctagagtc gatcgacaag ctcgagtttc tccataataa tgtgtgagta gttcccagat
    10621 aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag
    10681 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
    10741 tccagtacta aaatccagat cgctcagatg agaccgcagc tggcacgaca ggtttcccga
    10801 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    10861 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    10921 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
    10981 ctctagagga tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg
    11041 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
    11101 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
    11161 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
    11221 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
    11281 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
    11341 gacaagctgt gacggtctca gggacacgaa gtgatccgtt taaactatca gtgtttgaca
    11401 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
    11461 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgccagccgc ctttgcgacg
    11521 ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
    11581 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc
    11641 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
    11701 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
    11761 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
    11821 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
    11881 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
    11941 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
    12001 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
    12061 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
    12121 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
    12181 cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg ctggccaatt cccgagtgcg
    12241 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    12301 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatggcta aaatgagaat
    12361 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat
    12421 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac
    12481 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg
    12541 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag
    12601 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca
    12661 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga
    12721 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt
    12781 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaggaca ctccatttaa
    12841 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt
    12901 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt
    12961 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg
    13021 gtcgctcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg
    13081 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagct
    13141 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    13201 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    13261 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    13321 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    13381 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    13441 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    13501 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    13561 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    13621 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    13681 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    13741 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    13801 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    13861 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    13921 acggttcctg gcagatccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc
    13981 gacttcttcc gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc
    14041 aaggggtcgc tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc
    14101 cagacggtct acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca
    14161 ccaggcgggt caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg
    14221 caaggagggt gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac
    14281 gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc
    14341 cgcgaaacct tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc
    14401 gacagcgtgc aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg
    14461 cgtcgtcttg aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga
    14521 actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag
    14581 gccaagcagg ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc
    14641 ttgttcgata ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc
    14701 tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc
    14761 attttccacg tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac
    14821 gatgacgaac tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag
    14881 ccgatcacct tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg
    14941 tattacacga aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg
    15001 tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac
    15061 cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt
    15121 gctggcgacc actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc
    15181 cgacggatgt tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc
    15241 ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc
    15301 gaagcctgcg aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac
    15361 ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc
    15421 cctgctcgga tctgttggac cggacagtag tcatggttga tgggctgcct gtatcgagtg
    15481 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    15541 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    15601 actggggttg aacactct
//
