LOCUS       sequence-378287-       13523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the AND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13523)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 13523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP750_L2pV1_AND_B3RT_FRT_Rluc_1IB_lacZ
FEATURES             Location/Qualifiers
     source          1..13523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      878..1585
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1649..1682
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      1683..2390
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    2391..2424
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          2491..2679
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    3707..3881
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             4269..5921
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4335..4353)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5941..6141
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    6484..6539
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6544..6564
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7342..7530
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    8492..8666
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    8736..8757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8772..8777,8778..8795,8796..8802)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8810..8826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8815..8837
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8834..8850
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8834..8850
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8846..9169
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8863..8919
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8920..8937)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8920..8936)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8929..8951)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9145..9164)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    9301..9325
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9413..10123
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10144..10248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10249..11043
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11214..11802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11703..11722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12138..13286
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13432..13456
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaaggttgc ttaagaataa gtaattctta agcaaccctg ctttaatgag atatgcgaga
      901 agcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
      961 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     1021 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     1081 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     1141 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     1201 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     1261 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     1321 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     1381 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     1441 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     1501 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     1561 gcgacaacat gtcgaggctc agcagggttg cttaagaata agtaattctt aagcaacctc
     1621 tgtagagtga acttcaccac atattctgga agttcctatt ctctagaaag tataggaact
     1681 tcctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct ttcaattctg
     1741 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
     1801 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
     1861 aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg
     1921 tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt
     1981 ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga
     2041 ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg
     2101 agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt
     2161 gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca
     2221 acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt
     2281 ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc
     2341 gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag gaagttccta
     2401 ttctctagaa agtataggaa cttccaacaa acaacattac aattactatt tacaattaca
     2461 atgacttcaa aggtgtatga tcctgagcag gtaagtttct gcttctacct ttgatatata
     2521 tataataatt atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa
     2581 agaatgtagt atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat
     2641 aacttttcta atatatgacc aaaatttgtt gatgtgcaga gaaaaagaat gataactggt
     2701 ccacaatggt gggcaagatg taaacagatg aacgtgcttg attctttcat taattactac
     2761 gattcagaaa agcacgctga gaatgcagtt atctttcttc acggaaacgc tgcatcttca
     2821 tatttgtgga gacatgttgt gcctcacatt gaaccagtgg ctaggtgtat tatcccagat
     2881 cttatcggaa tgggaaagtc tggtaaatct ggaaatggtt cttacagact tttggatcat
     2941 tataagtacc tcacagcttg gttcgaactt ctcaacttac ctaagaagat tattttcgtt
     3001 ggacacgatt ggggtgcttg cttggcattc cattactcat acgaacacca agataagatc
     3061 aaagctatag ttcatgcaga gagtgttgtg gatgtgatag aatcttggga tgagtggcca
     3121 gatattgaag aggatattgc tttgatcaag tctgaagagg gagaaaagat ggttctcgaa
     3181 aataactttt tcgtggaaac tatgcttcct tcaaagatta tgagaaaatt ggaaccagaa
     3241 gagtttgctg catatctcga acctttcaag gaaaaaggag aggttagaag gccaacactc
     3301 agttggccta gggaaatacc attagttaag ggaggtaaac ctgatgttgt gcagatcgtg
     3361 agaaattata acgcttacct tagggcatca gatgatttgc ctaagatgtt catcgagtca
     3421 gatccaggat ttttcagtaa tgctatagtt gaaggtgcaa agaaatttcc aaacaccgag
     3481 ttcgttaagg tgaaaggatt gcatttctca caagaagatg ctcctgatga gatgggaaag
     3541 tatattaagt cttttgttga aagagtgctc aaaaacgagc agggttctcg atctgatgct
     3601 tctgaagtaa cgtcaacaag ctcccagtct gaggtatgca cagtggaaac gccaggttgt
     3661 gttggttctt aagcttctct agctagagtc gatcgacaag ctcgagtttc tccataataa
     3721 tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg
     3781 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     3841 tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctgcaaga attcaagctt
     3901 ggagccaaca ctcgaatccc cacccgttgg accaaacccg gctcattaag cgtcggttca
     3961 gatttatttc ctttatttaa aaaaaaggaa agggtaaaaa atagaaaatt ggaaacagtt
     4021 aaagcccaaa attgtaattt accgagaatt gtaaatttac ctgaaaaccc tacgctatag
     4081 tttcgactat aaataccaaa cttaggacct cacttcagaa tcccctcgtc gctgcgtctc
     4141 tctcccgcaa ccttcgattt tcgtttattc gcatccatcg gagagagaaa acaatcaata
     4201 agcgacctac tacaaacaag taagtaagag aaaaaccaaa agaagaagag aaacaacaag
     4261 aagaagtaat ggaggacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag
     4321 aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     4381 gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg
     4441 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     4501 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     4561 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     4621 tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga
     4681 acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct aaaacggatt
     4741 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     4801 aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt
     4861 cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca
     4921 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     4981 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     5041 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc
     5101 aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa
     5161 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac
     5221 ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac
     5281 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     5341 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     5401 ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta
     5461 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     5521 tacattctgg agacatagct tactgggacg aggacgaaca cttcttcata gttgaccgct
     5581 tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat
     5641 tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tctgcccgac gatgacgccg
     5701 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     5761 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     5821 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     5881 tcctcataaa ggccaagaag ggcggaaagt ccaaattgta agcttgatcc cccgtcgaca
     5941 gctagctata tcatcaattt atgtattaca cataatatcg cactcagtct ttcatctacg
     6001 gcaatgtacc agctgatata atcagttatt gaaatatttc tgaatttaaa cttgcatcaa
     6061 taaatttatg tttttgcttg gactataata cctgacttgt tattttatca ataaatattt
     6121 aaactatatt tctttcaaga tcgctactag aattcgagct cggagccaac actcgaatcc
     6181 ccacccgttg gaccaaaccc ggctcattaa gcgtcggttc agatttattt cctttattta
     6241 aaaaaaagga aagggtaaaa aatagaaaat tggaaacagt taaagcccaa aattgtaatt
     6301 taccgagaat tgtaaattta cctgaaaacc ctacgctata gtttcgacta taaataccaa
     6361 acttaggacc tcacttcaga atcccctcgt cgctgcgtct ctctcccgca accttcgatt
     6421 ttcgtttatt cgcatccatc ggagagagaa aacaatcaat aagcgaccta ctgtattttt
     6481 acaacaatta ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca
     6541 atgcccaaga aaaagcggaa ggtgtccagc tacatggacc tggtggacga cgagcccgcc
     6601 accctgtacc acaagttcgt ggaatgcctg aaggccggcg agaacttctg cggcgataag
     6661 ctgagcggca tcatcaccat ggccattctg aaggccatca aggccctgac cgaagtgaag
     6721 aaaaccacct tcaacaagta caagaccacc atcaagcagg gcctgcagta cgacgtgggc
     6781 agcagcacca tcagcttcgt gtaccacctg aaggactgcg acgagctgag cagaggcctg
     6841 agcgacgcct tcgagcccta caagttcaag atcaagagca acaaagaggc caccagcttc
     6901 aagaccctgt tcaggggccc tagcttcggc agccagaaga actggcggaa gaaagaggtg
     6961 gaccgcgagg tggacaacct gttccacagc accgagacag acgagagcat cttcaagttc
     7021 atcctgaaca ccctggacag catcgaaacc cagaccaaca ccgaccggca gaaaaccgtg
     7081 ctgaccttta tcctgctgat gaccttcttc aactgctgcc ggaacaacga cctgatgaac
     7141 gtggacccca gcaccttcaa gatcgtgaag aacaagtttg tgggctacct gctgcaggct
     7201 gaagtgaagc agaccaagac cagaaagagc cggaatatct tcttcttccc catccgggaa
     7261 aaccgcttcg acctgttcct ggccctgcac gacttcttca gaacctgcca gcccaccccc
     7321 aagagcagac tgagcgatca ggtaagtttc tgcttctacc tttgatatat atataataat
     7381 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag
     7441 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct
     7501 aatatatgac caaaatttgt tgatgtgcag gtgtccgagc agaagtggca gctgttccgg
     7561 gacagcatgg tcatcgacta caaccggttc tttcggaagt tccccgccag ccccatcttc
     7621 gccattaagc acggccccaa gtcccacctg ggccggcatc tgatgaacag ctttctgcac
     7681 aagaacgagc tggacagctg ggccaacagc ctgggcaatt ggagcagctc ccagaaccag
     7741 agagagagcg gcgccagact gggctacaca cacggcggaa gagatctgcc ccagcccctg
     7801 tttggcttcc tggccggata ctgcgtgcgg aacgaagagg gccacatcgt gggcctgggc
     7861 ctggaaaagg acatcaacga tctgttcgac ggcatcatgg accccctgaa cgagaaagag
     7921 gacaccgaga tctgcgagag ctacggcgag tgggccaaga ttgtgtccaa ggacgtgctg
     7981 atcttcctga agagatacca cagcaagaac gcctgtcgga gataccagaa cagcaccctg
     8041 tatgcccgga ccttcctgaa aaccgagagc gtgaccctga gcggctccaa gggcagcgag
     8101 gaaccttcta gccctgtgcg gatccccatc ctgagcatgg gaaaggccag cccctccgag
     8161 ggaagaaagc tgagagccag cgagcacgcc aacgacgaca acgagatcga gaagatcgac
     8221 agcgacagca gccagagcga agagatccct atcgagatga gcgactccga ggacgagaca
     8281 accgccagca acatcagcgg catctacctg gacatgagca aggccaactc caacgtggtg
     8341 tacagccccc ctagccagac aggcagagct gctggcgccg gaagaaaaag aggcgtggga
     8401 ggcagacgga ccgtggaaag caagcggaga agagtgctgg cccccatcaa ccggtgagct
     8461 tctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
     8521 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
     8581 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     8641 caaaatccag tactaaaatc cagatcgctt tactgagacc gcagctggca cgacaggttt
     8701 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8761 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8821 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc atgcctgcag
     8881 gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg
     8941 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     9001 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     9061 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     9121 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     9181 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     9241 ttacagacaa gctgtgacgg tctcagggac acgaagtgat ccgtttaaac tatcagtgtt
     9301 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     9361 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca gccgcctttg
     9421 cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa
     9481 acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat
     9541 acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg
     9601 ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt
     9661 ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga
     9721 tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta
     9781 ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg
     9841 cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt
     9901 tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta
     9961 taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg
    10021 gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg
    10081 ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc caattcccga
    10141 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    10201 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat ggctaaaatg
    10261 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
    10321 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
    10381 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
    10441 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc
    10501 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
    10561 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
    10621 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat
    10681 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga ggacactcca
    10741 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
    10801 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
    10861 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
    10921 gtccggtcgc tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta
    10981 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
    11041 tagctgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    11101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    11161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    11221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    11281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    11341 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    11401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    11461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    11521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    11581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    11641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    11701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    11761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    11821 tttttacggt tcctggcaga tcctagatgt ggcgcaacga tgccggcgac aagcaggagc
    11881 gcaccgactt cttccgcatc aagtgttttg gctctcaggc cgaggcccac ggcaagtatt
    11941 tgggcaaggg gtcgctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg
    12001 acggccagac ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca
    12061 aggcaccagg cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa
    12121 tcccgcaagg agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga
    12181 tcgacgcggg gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg
    12241 cgccccgcga aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg
    12301 agcgcgacag cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc
    12361 gttcgcgtcg tcttgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc
    12421 gaggaactat gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca
    12481 gcgaggccaa gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc
    12541 tttccttgtt cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg
    12601 cccgctctgc cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca
    12661 aggtcatttt ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg
    12721 ccgacgatga cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg
    12781 gcgagccgat caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg
    12841 gccggtatta cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct
    12901 tcacgtccga ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc
    12961 tggaccgtgg caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc
    13021 tgtttgctgg cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga
    13081 cggcccgacg gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg
    13141 aaaccttccg cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg
    13201 tcggcgaagc ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg
    13261 atgacctggt gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag
    13321 cagcccctgc tcggatctgt tggaccggac agtagtcatg gttgatgggc tgcctgtatc
    13381 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
    13441 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
    13501 aatgtactgg ggttgaacac tct
//
