LOCUS       sequence-378296-       17179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test if a single plasmid stably transformed into Arabidopsis can 
            switched from off to on when its AND gate unit is activated by both 
            cell type (cortex) and chemical induction (by DEX)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17179)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 17179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP793_L2_V05_s35S_AND_GFP_pOp6_Flp_CO2_B3
FEATURES             Location/Qualifiers
     source          1..17179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..444
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      517..1224
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1288..1321
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      1322..2029
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    2030..2063
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(2788..2805)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     polyA_signal    3076..3250
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             4453..5148
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     promoter        5692..6037
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             6102..6809
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6247..6265)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      7060..7260
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    7351..7370
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7403..7422
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7455..7474
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7507..7526
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7559..7578
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    7611..7630
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        7654..7700
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    7716..7771
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             7779..7799
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8483..8671
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    9295..9469
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             10137..10157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          10935..11123
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      12063..12315
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    12392..12413
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12428..12433,12434..12451,12452..12458)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12466..12482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12471..12493
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12490..12506
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12490..12506
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             12502..12825
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    12519..12575
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(12576..12593)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12576..12592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12585..12607)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12801..12820)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    12957..12981
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      13069..13779
                     /label=oriV
                     /note="incP origin of replication"
     promoter        13800..13904
                     /gene="bla"
                     /label=AmpR promoter
     CDS             13905..14699
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14870..15458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15359..15378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             15794..16942
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    17088..17112
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggtcaacatg gtggagcacg acactctggt ctactccaaa
       61 aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca acaaaggata
      121 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
      181 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
      241 caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      301 gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga catctccact
      361 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      421 agttcatttc atttggagag gacacgctac tgtattttta caacaattac caacaacaac
      481 aaaggttgct taagaataag taattcttaa gcaaccctgc tttaatgaga tatgcgagaa
      541 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
      601 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
      661 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt
      721 atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct
      781 atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa
      841 aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa
      901 aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga
      961 agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc
     1021 cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta
     1081 accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga
     1141 agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg
     1201 cgacaacatg tcgaggctca gcagggttgc ttaagaataa gtaattctta agcaacctct
     1261 gtagagtgaa cttcaccaca tattctggaa gttcctattc tctagaaagt ataggaactt
     1321 cctgctttaa tgagatatgc gagaagccta tgatcgcatg atatttgctt tcaattctgt
     1381 tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt
     1441 cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca
     1501 atttactgat tgtaccctac tacttatatg tacaatatta aaatgaaaac aatatattgt
     1561 gctgaatagg tttatagcga catctatgat agagcgccac aataacaaac aattgcgttt
     1621 tattattaca aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat
     1681 tacataaatc ttattcaaat ttcaaaagtg ccccaggggc tagtatctac gacacaccga
     1741 gcggcgaact aataacgctc actgaaggga actccggttc cccgccggcg cgcatgggtg
     1801 agattccttg aagttgagta ttggccgtcc gctctaccga aagttacggg caccattcaa
     1861 cccggtccag cacggcggcc gggtaaccga cttgctgccc cgagaattat gcagcatttt
     1921 tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg
     1981 ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg aagttcctat
     2041 tctctagaaa gtataggaac ttccaacaaa caacattaca attactattt acaattacaa
     2101 tgagaggatc tggatctgag tctgatgagt ctggacttcc tgctatggaa atcgagtgta
     2161 gaatcactgg aacccttaac ggtgttgagt tcgagcttgt tggaggtggt gagggaactc
     2221 ctgagcaggg aagaatgact aacaagatga agtctaccaa gggtgctctt accttctctc
     2281 cataccttct ttctcacgtt atgggatacg gattctacca cttcggaact tacccatctg
     2341 gatacgagaa ccctttcctt catgctatca acaacggtgg atacaccaac actaggatcg
     2401 agaagtacga ggatggtggt gttcttcacg ttagcttctc ttacagatac gaggctggaa
     2461 gagtgatcgg agatttcaag gttatgggaa ctggattccc tgaggattct gttatcttca
     2521 ccgacaagat catcaggtct aacgctactg ttgagcatct tcatcctatg ggagataacg
     2581 atctcgatgg atctttcacc agaaccttct cacttagaga tggtggttac tactcttctg
     2641 tggtggattc tcacatgcac ttcaagtctg ctatccaccc ttctatcctt caaaacggtg
     2701 gacctatgtt cgctttcaga agagttgagg aagatcactc taacaccgag cttggaatcg
     2761 ttgagtacca acatgctttc aagacccctg atgctgatgc tggtgaggaa ttcgaagagc
     2821 aagcaaggaa agctaaggtg aacaatgaga aaaagacgga aatagtgaaa ccagagagtt
     2881 gtagcaatga aggagatgtc aaggatctga aaagaaagga ctctgaggat ggaaacgagg
     2941 gtgaggaaga agaagcttct tcgaaaccga aaaagccaaa agttgctctt tctcatcttc
     3001 aggacattga cgacacagaa gctgatcaag aagaagagta agcttctcta gctagagtcg
     3061 atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag
     3121 ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     3181 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa
     3241 aatccagatc gctgcaagaa ttcaagcttg gagttctaga atgtcgcgga acaaatttta
     3301 aaactaaatc ctaaattttt ctaattttgt tgccaatagt ggatatgtgg gccgtataga
     3361 aggaatctat tgaaggccca aacccatact gacgagccca aaggttcgtt ttgcgtttta
     3421 tgtttcggtt cgatgccaac gccacattct gagctaggca aaaaacaaac gtgtctttga
     3481 atagactcct ctcgttaaca catgcagcgg ctgcatggtg acgccattaa cacgtggcct
     3541 acaattgcat gatgtctcca ttgacacgtg acttctcgtc tcctttctta atatatctaa
     3601 caaacactcc tacctcttcc aaaatatata cacatctttt tgatcaatct ctcattcaaa
     3661 atctcattct ctctagtaaa caagaacaaa aaaatggcgg atacagctag aggaacccat
     3721 cacgatatca tcggcagaga tcagtacccg atgatgggcc gagatcgtga ccagtaccag
     3781 atgtccggac gaggatctga ctactccaag tctaggcaga ttgctaaagc tgcaactgct
     3841 gtcacagctg gtggttccct ccttgttctc tccagcctta cccttgttgg aactgtcata
     3901 gctttgactg ttgcaacacc tctgctcgtt atcttcagcc caatccttgt cccggctctc
     3961 atcacagttg cactcctcat caccggtttt ctttcctctg gagggtttgg cattgccgct
     4021 ataaccgttt tctcttggat ttacaagtaa gcacacattt atcatcttac ttcataattt
     4081 tgtgcaatat gtgcatgcat gtgttgagcc agtagctttg gatcaatttt tttggtcgaa
     4141 taacaaatgt aacaataaga aattgcaaat tctagggaac atttggttaa ctaaatacga
     4201 aatttgacct agctagcttg aatgtgtctg tgtatatcat ctatataggt aaaatgcttg
     4261 gtatgatacc tattgattgt gaataggtac gcaacgggag agcacccaca gggatcagac
     4321 aagttggaca gtgcaaggat gaagttggga agcaaagctc aggatctgaa agacagagct
     4381 cagtactacg gacagcaaca tactggtggg gaacatgacc gtgaccgtac tcgtggtggc
     4441 cagcacacta ctatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     4501 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     4561 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     4621 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaaccacac ccagggcatc
     4681 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     4741 gaagatgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     4801 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     4861 acactcggct gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctggaaggc
     4921 agaagcgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttcaagacc
     4981 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
     5041 cacagactgg aaagaatcaa ggaggccgac aaagaaacct acgtcgagca gcacgaggtg
     5101 gctgtggcca gatactgcga cctccctagc aaactggggc acaagtgagc ttaccccact
     5161 gatgtcatcg tcatagtcca ataactccaa tgtcggggag ttagtttatg aggaataaag
     5221 tgtttagaat ttgatcaggg ggagataata aaagccgagt ttgaatcttt ttgttataag
     5281 taatgtttat gtgtgtttct atatgttgtc aaatggtacc atgttttttt tcctctcttt
     5341 ttgtaacttg caagtgttgt gttgtacttt atttggcttc tttgtaagtt ggtaacggtg
     5401 gtctatatat ggaaaaggtc ttgttttgtt aaacttatgt tagttaactg gattcgtctt
     5461 taaccacaaa aagttttcaa taagctacaa atttagacac gcaagccgat gcagtcatta
     5521 gtacatatat ttattgcaag tgattacatg gcaacccaaa cttcaaaaac agtaggttgc
     5581 tccatttagt cgctactaga attcgagctc ggaggtcaac atggtggagc acgacactct
     5641 ggtctactcc aaaaatgtca aagatacagt ctcagaagat caaagggcta ttgagacttt
     5701 tcaacaaagg ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
     5761 catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     5821 aaaggctatc attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac
     5881 gaggagcatc gtggaaaaag aagaggttcc aaccacgtct acaaagcaag tggattgatg
     5941 tgacatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     6001 ctctatataa ggaagttcat ttcatttgga gaggacacgc tactacaaac aagtaagtaa
     6061 gagaaaaacc aaaagaagaa gagaaacaac aagaagaagt aatggtgagc aagggcgagg
     6121 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     6181 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     6241 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     6301 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     6361 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     6421 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     6481 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aaaaccatgg
     6541 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     6601 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     6661 aggccaagaa gcccgtgcag ctgccaggcg cctacaacgt caacatcaag ttggacatca
     6721 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     6781 ccaccggcgg catggacgag ctgtacaagt tcgaagagca agcaaggaaa gctaaggtga
     6841 acaatgagaa aaagacggaa atagtgaaac cagagagttg tagcaatgaa ggagatgtca
     6901 aggatctgaa aagaaaggac tctgaggatg gaaacgaggg tgaggaagaa gaagcttctt
     6961 cgaaaccgaa aaagccaaaa gttgctcttt ctcatcttca ggacattgac gacacagaag
     7021 ctgatcaaga agaagagtaa gcttgatccc ccgtcgacag ctagctatat catcaattta
     7081 tgtattacac ataatatcgc actcagtctt tcatctacgg caatgtacca gctgatataa
     7141 tcagttattg aaatatttct gaatttaaac ttgcatcaat aaatttatgt ttttgcttgg
     7201 actataatac ctgacttgtt attttatcaa taaatattta aactatattt ctttcaagat
     7261 cgctttacga attcccatgg ggagcggccg catgcatatg tcgaggaatt cgagctcaag
     7321 aaatctagaa agaagaaagg gaagagaaag aattgtgagc gctcacaatt gaaagactag
     7381 aaagaagaaa gggaagagaa agaattgtga gcgctcacaa ttgaaagact agaaagaaga
     7441 aagggaagag aaagaattgt gagcgctcac aattgaaaga ctagaaagaa gaaagggaag
     7501 agaaagaatt gtgagcgctc acaattgaaa gactagaaag aagaaaggga agagaaagaa
     7561 ttgtgagcgc tcacaattga aagactagaa agaagaaagg gaagagaaag aattgtgagc
     7621 gctcacaatt gaaagactag tggatcgatc ttcgcaagac ccttcctcta tataaggaag
     7681 ttcatttcat ttggagagga tactgtattt ttacaacaat taccaacaac aacaaacaac
     7741 aaacaacatt acaattacta tttacaatta caatggctcc taagaagaag aggaaggtga
     7801 tgagccagtt cgacatcctg tgcaagaccc cccccaaggt gctggtgcgg cagttcgtgg
     7861 agagattcga gaggcccagc ggcgagaaga tcgccagctg tgccgccgag ctgacctacc
     7921 tgtgctggat gatcacccac aacggcaccg ccatcaagag ggccaccttc atgagctaca
     7981 acaccatcat cagcaacagc ctgagcttcg acatcgtgaa caagagcctg cagttcaagt
     8041 acaagaccca gaaggccacc atcctggagg ccagcctgaa gaagctgatc cccgcctggg
     8101 agttcaccat catcccttac aacggccaga agcaccagag cgacatcacc gacatcgtgt
     8161 ccagcctgca gctgcagttc gagagcagcg aggaggccga caagggcaac agccacagca
     8221 agaagatgct gaaggccctg ctgtccgagg gcgagagcat ctgggagatc accgagaaga
     8281 tcctgaacag cttcgagtac accagcaggt tcaccaagac caagaccctg taccagttcc
     8341 tgttcctggc cacattcatc aactgcggca ggttcagcga catcaagaac gtggacccca
     8401 agagcttcaa gctggtgcag aacaagtacc tgggcgtgat cattcagtgc ctggtgaccg
     8461 agactaagac aagcgtgtcc aggtaagttt ctgcttctac ctttgatata tatataataa
     8521 ttatcattaa ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta
     8581 gtatatagca attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc
     8641 taatatatga ccaaaatttg ttgatgtgca ggcacatcta ctttttcagc gccagaggca
     8701 ggatcgaccc cctggtgtac ctggacgagt tcctgaggaa cagcgagccc gtgctgaaga
     8761 gagtgaacag gaccggcaac agcagcagca acaagcagga gtaccagctg ctgaaggaca
     8821 acctggtgcg cagctacaac aaggccctga agaagaacgc cccctacccc atcttcgcta
     8881 tcaagaacgg ccctaagagc cacatcggca ggcacctgat gaccagcttt ctgagcatga
     8941 agggcctgac cgagctgaca aacgtggtgg gcaactggag cgacaagagg gcctccgccg
     9001 tggccaggac cacctacacc caccagatca ccgccatccc cgaccactac ttcgccctgg
     9061 tgtccaggta ctacgcctac gaccccatca gcaaggagat gatcgccctg aaggacgaga
     9121 ctaaccccat cgaggagtgg cagcacatcg agcagctgaa gggcagcgcc gagggcagca
     9181 tcagataccc cgcctggaac ggcatcatca gccaggaggt gctggactac ctgagcagct
     9241 acatcaacag gcggatctga gcttctctag ctagagtcga tcgacaagct cgagtttctc
     9301 cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc
     9361 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
     9421 ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcg ctcagagaat
     9481 tcgcatgcgg aggaattcgc atgcggaggt agctttgctt ttttctctat taagaatgta
     9541 cggacaacga aaccctctac gttattaaga agcgttcact gtttgttgtc aagtcttttc
     9601 aagtttcaac aaaatgtcat tagaaataaa gttaaataca taagagaaca aacaatttca
     9661 cggactcttt agttcggaga cagtgatcat aatctgatat atactttgta gtcacgggct
     9721 tgaccttgaa gtttgcatcg gaccaacaac gatccaaaaa acctttagtt gtaacgcaat
     9781 aaaatgctcc aatatttctc gaagtttgga acatcttctt ttttcttcat tttcttgcct
     9841 tacacatgtt cgtttgtacg taccataaca aataatcaaa tctactacac ttataaacac
     9901 tatacctatt tatgtgcgta gtggttaaca cccatacaag agtcacgggt cacatttaag
     9961 cttcaactat atatttgcat gttatttgcc aaaaggattt gacaataatg caacaagagt
    10021 ttcattgaaa atttcaagaa ccctagttaa taacgatata ctacaccacc aacacaacca
    10081 aacccaccac gcccaattgt tacacacccg cttggaaaag caagtctaac aaaatgccca
    10141 agaaaaagcg gaaggtgtcc agctacatgg acctggtgga cgacgagccc gccaccctgt
    10201 accacaagtt cgtggaatgc ctgaaggccg gcgagaactt ctgcggcgat aagctgagcg
    10261 gcatcatcac catggccatt ctgaaggcca tcaaggccct gaccgaagtg aagaaaacca
    10321 ccttcaacaa gtacaagacc accatcaagc agggcctgca gtacgacgtg ggcagcagca
    10381 ccatcagctt cgtgtaccac ctgaaggact gcgacgagct gagcagaggc ctgagcgacg
    10441 ccttcgagcc ctacaagttc aagatcaaga gcaacaaaga ggccaccagc ttcaagaccc
    10501 tgttcagggg ccctagcttc ggcagccaga agaactggcg gaagaaagag gtggaccgcg
    10561 aggtggacaa cctgttccac agcaccgaga cagacgagag catcttcaag ttcatcctga
    10621 acaccctgga cagcatcgaa acccagacca acaccgaccg gcagaaaacc gtgctgacct
    10681 ttatcctgct gatgaccttc ttcaactgct gccggaacaa cgacctgatg aacgtggacc
    10741 ccagcacctt caagatcgtg aagaacaagt ttgtgggcta cctgctgcag gctgaagtga
    10801 agcagaccaa gaccagaaag agccggaata tcttcttctt ccccatccgg gaaaaccgct
    10861 tcgacctgtt cctggccctg cacgacttct tcagaacctg ccagcccacc cccaagagca
    10921 gactgagcga tcaggtaagt ttctgcttct acctttgata tatatataat aattatcatt
    10981 aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag
    11041 caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat
    11101 gaccaaaatt tgttgatgtg caggtgtccg agcagaagtg gcagctgttc cgggacagca
    11161 tggtcatcga ctacaaccgg ttctttcgga agttccccgc cagccccatc ttcgccatta
    11221 agcacggccc caagtcccac ctgggccggc atctgatgaa cagctttctg cacaagaacg
    11281 agctggacag ctgggccaac agcctgggca attggagcag ctcccagaac cagagagaga
    11341 gcggcgccag actgggctac acacacggcg gaagagatct gccccagccc ctgtttggct
    11401 tcctggccgg atactgcgtg cggaacgaag agggccacat cgtgggcctg ggcctggaaa
    11461 aggacatcaa cgatctgttc gacggcatca tggaccccct gaacgagaaa gaggacaccg
    11521 agatctgcga gagctacggc gagtgggcca agattgtgtc caaggacgtg ctgatcttcc
    11581 tgaagagata ccacagcaag aacgcctgtc ggagatacca gaacagcacc ctgtatgccc
    11641 ggaccttcct gaaaaccgag agcgtgaccc tgagcggctc caagggcagc gaggaacctt
    11701 ctagccctgt gcggatcccc atcctgagca tgggaaaggc cagcccctcc gagggaagaa
    11761 agctgagagc cagcgagcac gccaacgacg acaacgagat cgagaagatc gacagcgaca
    11821 gcagccagag cgaagagatc cctatcgaga tgagcgactc cgaggacgag acaaccgcca
    11881 gcaacatcag cggcatctac ctggacatga gcaaggccaa ctccaacgtg gtgtacagcc
    11941 cccctagcca gacaggcaga gctgctggcg ccggaagaaa aagaggcgtg ggaggcagac
    12001 ggaccgtgga aagcaagcgg agaagagtgc tggcccccat caaccggtga gcttgtcaag
    12061 cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
    12121 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
    12181 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
    12241 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
    12301 ctatgttact agatcgacgc tcgcttgtgt gagaccgcag ctggcacgac aggtttcccg
    12361 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    12421 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    12481 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg
    12541 actctagagg atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt
    12601 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    12661 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    12721 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
    12781 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
    12841 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
    12901 agacaagctg tgacggtctc agggacacga agtgatccgt ttaaactatc agtgtttgac
    12961 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
    13021 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgccagccg cctttgcgac
    13081 gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc
    13141 gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct
    13201 cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga
    13261 ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag
    13321 ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat
    13381 gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga
    13441 cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca
    13501 cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg
    13561 cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa
    13621 ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc
    13681 ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc
    13741 gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggccaat tcccgagtgc
    13801 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    13861 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa
    13921 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
    13981 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
    14041 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
    14101 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
    14161 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
    14221 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
    14281 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
    14341 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta
    14401 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
    14461 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
    14521 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
    14581 ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
    14641 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc
    14701 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    14761 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    14821 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    14881 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    14941 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    15001 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    15061 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    15121 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    15181 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    15241 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    15301 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    15361 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    15421 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    15481 tacggttcct ggcagatcct agatgtggcg caacgatgcc ggcgacaagc aggagcgcac
    15541 cgacttcttc cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg
    15601 caaggggtcg ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg
    15661 ccagacggtc tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc
    15721 accaggcggg tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc
    15781 gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga
    15841 cgcggggttt tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc
    15901 ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg
    15961 cgacagcgtg caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc
    16021 gcgtcgtctt gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg
    16081 aactatgacg accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga
    16141 ggccaagcag gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc
    16201 cttgttcgat attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg
    16261 ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt
    16321 cattttccac gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga
    16381 cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga
    16441 gccgatcacc ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg
    16501 gtattacacg aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac
    16561 gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga
    16621 ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt
    16681 tgctggcgac cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc
    16741 ccgacggatg ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac
    16801 cttccgcctc atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg
    16861 cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga
    16921 cctggtgcat tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc
    16981 ccctgctcgg atctgttgga ccggacagta gtcatggttg atgggctgcc tgtatcgagt
    17041 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    17101 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    17161 tactggggtt gaacactct
//
