LOCUS       sequence-378298-       12128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the on state for testing of the alt SCre recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP671_L2_pV01_1I_SloxM1-OCS-Rluc_TCTP_SCre_lacZ
FEATURES             Location/Qualifiers
     source          1..12128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5680..5735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5740..5760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6079..6267
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7097..7271
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7341..7362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7377..7382,7383..7400,7401..7407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7415..7431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7420..7442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7439..7455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7439..7455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7451..7774
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7468..7524
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7525..7542)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7525..7541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7534..7556)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7750..7769)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    7906..7930
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8018..8728
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8749..8853
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8854..9648
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9819..10407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10308..10327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10743..11891
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12037..12061
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaactcgtg tccgataact gtaattatcg gacacgagct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcagctcg tgtccgataa ctgtaattat cggacacgag
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct
     5401 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
     5461 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
     5521 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
     5581 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
     5641 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
     5701 caacaaacaa cattacaatt actatttaca attacaatgc ccaagaaaaa gcggaaggtg
     5761 tccctgctga ccaccaacaa ccacagcgtg gccctgagct acggcgagcc tcctagcacc
     5821 ctgaacgaca gcctgaagga cagctaccag cggagcaccg atgagctgca ggccctgctg
     5881 tctaagcctc tggcccagct gaccgacgcc gacaagctgc ggatcagaga gatcacccag
     5941 gccaagctga agcacttcct ggacaacggc caccggacca gaagggccaa cacttggaga
     6001 gccctgatga gcagatgggc caagttcgag agctggtgcc tgaccaacaa tctgaccccc
     6061 ctgcctgcca cccctgaggt aagtttctgc ttctaccttt gatatatata taataattat
     6121 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     6181 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     6241 atatgaccaa aatttgttga tgtgcaggtg gtggccacat tcatcgagta ctaccaggcc
     6301 agcagctaca ccaccctgag ccagtatgcc tgggccatca acagctttca cgtggaatgc
     6361 ggcctgctga gccccgtgtc tagcaagacc gtgcaggaca agcagaacga gatcagaatc
     6421 gtgaagctgg aatctggcgg cctggcccag gaacaggcca ccccttttag actgcaccat
     6481 ctgcagatgc tgatcgagag ctatggcgag agcgagcggc tgctggacaa gagaaacctg
     6541 gctctgctga atatcgccta cgagagcctg ctgcgcgagt ccgagctgct gagaatcaaa
     6601 gtgggccacc tgaagtccac cttcgagggc gactacgtgc tgagcgtgcc ctacaccaag
     6661 accaacgaca gcggcgaaga ggaagtcgtg aacatcaccc ccctgggctt caagctgatc
     6721 cagcggtaca tccagggcgc tggcctgaca aaagaggact acctgttcca gcccatcggc
     6781 cggtccaaca aggtgtccgt gcaggccaaa cccatgagca cccggaccgt ggacagagtg
     6841 ttcctgtggg cctttgagag cctgggcatc gacagacaca gcgcttggag cggccacagc
     6901 gccagaattg gagccgctca ggatctgctg gccgctggct attctatcgc ccagatccag
     6961 gaaaacggcc gctggaagtc ccccatgatg gtgctgagat acggcaagga catcaaggcc
     7021 aaagaaagcg ccatggccaa gatgctggcc gagcggagat gagcttctct agctagagtc
     7081 gatcgacaag ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta
     7141 gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg
     7201 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta
     7261 aaatccagat cgctttactg agaccgcagc tggcacgaca ggtttcccga ctggaaagcg
     7321 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7381 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7441 ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga
     7501 tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7561 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7621 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7681 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     7741 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     7801 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     7861 gacggtctca gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg
     7921 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     7981 aaaaggttta tccgttcgtc catttgtatg tgccagccgc ctttgcgacg ctcaccgggc
     8041 tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc
     8101 cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc gcggaaaact
     8161 tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg
     8221 cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct
     8281 cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc
     8341 tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg
     8401 cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat
     8461 ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg
     8521 gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta
     8581 accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc
     8641 gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg cccctcggcc
     8701 gcgaacggcc tcaccccaaa aatggcagcg ctggccaatt cccgagtgcg cggaacccct
     8761 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8821 taaatgcttc aataatattg aaaaaggaag agtatggcta aaatgagaat atcaccggaa
     8881 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
     8941 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
     9001 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
     9061 aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc
     9121 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
     9181 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
     9241 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
     9301 gatctggccg atgtggattg cgaaaactgg gaagaggaca ctccatttaa agatccgcgc
     9361 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
     9421 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
     9481 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgctcagg
     9541 gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
     9601 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagct gtcagaccaa
     9661 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9721 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9781 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9841 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9901 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9961 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10021 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10081 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10141 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10201 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10261 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10321 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10381 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10441 gcagatccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc gacttcttcc
    10501 gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc aaggggtcgc
    10561 tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct
    10621 acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt
    10681 caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt
    10741 gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt
    10801 ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct
    10861 tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc
    10921 aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtcttg
    10981 aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga
    11041 ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg
    11101 ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata
    11161 ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt
    11221 tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg
    11281 tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac
    11341 tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct
    11401 tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga
    11461 aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg
    11521 ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga
    11581 aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc
    11641 actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt
    11701 tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca
    11761 tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg
    11821 aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt
    11881 gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc cctgctcgga
    11941 tctgttggac cggacagtag tcatggttga tgggctgcct gtatcgagtg gtgattttgt
    12001 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    12061 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actggggttg
    12121 aacactct
//
