LOCUS       sequence-378300-       10999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the 0-input state of the AND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 10999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP748_L2pV1_AND_B3RT_FRT_Rluc_0I_lacZ
FEATURES             Location/Qualifiers
     source          1..10999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      878..1585
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1649..1682
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      1683..2390
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    2391..2424
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          2491..2679
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    3707..3881
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             4269..5921
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4335..4353)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5941..6141
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    6212..6233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6248..6253,6254..6271,6272..6278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6286..6302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6291..6313
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6310..6326
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6310..6326
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6322..6645
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    6339..6395
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6396..6413)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6396..6412)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6405..6427)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6621..6640)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    6777..6801
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6889..7599
                     /label=oriV
                     /note="incP origin of replication"
     promoter        7620..7724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7725..8519
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8690..9278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9179..9198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9614..10762
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10908..10932
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaaggttgc ttaagaataa gtaattctta agcaaccctg ctttaatgag atatgcgaga
      901 agcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
      961 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     1021 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     1081 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     1141 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     1201 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     1261 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     1321 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     1381 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     1441 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     1501 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     1561 gcgacaacat gtcgaggctc agcagggttg cttaagaata agtaattctt aagcaacctc
     1621 tgtagagtga acttcaccac atattctgga agttcctatt ctctagaaag tataggaact
     1681 tcctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct ttcaattctg
     1741 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
     1801 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
     1861 aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg
     1921 tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt
     1981 ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga
     2041 ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg
     2101 agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt
     2161 gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca
     2221 acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt
     2281 ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc
     2341 gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag gaagttccta
     2401 ttctctagaa agtataggaa cttccaacaa acaacattac aattactatt tacaattaca
     2461 atgacttcaa aggtgtatga tcctgagcag gtaagtttct gcttctacct ttgatatata
     2521 tataataatt atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa
     2581 agaatgtagt atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat
     2641 aacttttcta atatatgacc aaaatttgtt gatgtgcaga gaaaaagaat gataactggt
     2701 ccacaatggt gggcaagatg taaacagatg aacgtgcttg attctttcat taattactac
     2761 gattcagaaa agcacgctga gaatgcagtt atctttcttc acggaaacgc tgcatcttca
     2821 tatttgtgga gacatgttgt gcctcacatt gaaccagtgg ctaggtgtat tatcccagat
     2881 cttatcggaa tgggaaagtc tggtaaatct ggaaatggtt cttacagact tttggatcat
     2941 tataagtacc tcacagcttg gttcgaactt ctcaacttac ctaagaagat tattttcgtt
     3001 ggacacgatt ggggtgcttg cttggcattc cattactcat acgaacacca agataagatc
     3061 aaagctatag ttcatgcaga gagtgttgtg gatgtgatag aatcttggga tgagtggcca
     3121 gatattgaag aggatattgc tttgatcaag tctgaagagg gagaaaagat ggttctcgaa
     3181 aataactttt tcgtggaaac tatgcttcct tcaaagatta tgagaaaatt ggaaccagaa
     3241 gagtttgctg catatctcga acctttcaag gaaaaaggag aggttagaag gccaacactc
     3301 agttggccta gggaaatacc attagttaag ggaggtaaac ctgatgttgt gcagatcgtg
     3361 agaaattata acgcttacct tagggcatca gatgatttgc ctaagatgtt catcgagtca
     3421 gatccaggat ttttcagtaa tgctatagtt gaaggtgcaa agaaatttcc aaacaccgag
     3481 ttcgttaagg tgaaaggatt gcatttctca caagaagatg ctcctgatga gatgggaaag
     3541 tatattaagt cttttgttga aagagtgctc aaaaacgagc agggttctcg atctgatgct
     3601 tctgaagtaa cgtcaacaag ctcccagtct gaggtatgca cagtggaaac gccaggttgt
     3661 gttggttctt aagcttctct agctagagtc gatcgacaag ctcgagtttc tccataataa
     3721 tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg
     3781 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     3841 tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctgcaaga attcaagctt
     3901 ggagccaaca ctcgaatccc cacccgttgg accaaacccg gctcattaag cgtcggttca
     3961 gatttatttc ctttatttaa aaaaaaggaa agggtaaaaa atagaaaatt ggaaacagtt
     4021 aaagcccaaa attgtaattt accgagaatt gtaaatttac ctgaaaaccc tacgctatag
     4081 tttcgactat aaataccaaa cttaggacct cacttcagaa tcccctcgtc gctgcgtctc
     4141 tctcccgcaa ccttcgattt tcgtttattc gcatccatcg gagagagaaa acaatcaata
     4201 agcgacctac tacaaacaag taagtaagag aaaaaccaaa agaagaagag aaacaacaag
     4261 aagaagtaat ggaggacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag
     4321 aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     4381 gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg
     4441 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     4501 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     4561 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     4621 tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga
     4681 acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct aaaacggatt
     4741 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     4801 aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt
     4861 cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca
     4921 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     4981 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     5041 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc
     5101 aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa
     5161 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac
     5221 ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac
     5281 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     5341 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     5401 ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta
     5461 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     5521 tacattctgg agacatagct tactgggacg aggacgaaca cttcttcata gttgaccgct
     5581 tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat
     5641 tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tctgcccgac gatgacgccg
     5701 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     5761 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     5821 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     5881 tcctcataaa ggccaagaag ggcggaaagt ccaaattgta agcttgatcc cccgtcgaca
     5941 gctagctata tcatcaattt atgtattaca cataatatcg cactcagtct ttcatctacg
     6001 gcaatgtacc agctgatata atcagttatt gaaatatttc tgaatttaaa cttgcatcaa
     6061 taaatttatg tttttgcttg gactataata cctgacttgt tattttatca ataaatattt
     6121 aaactatatt tctttcaaga tcgctactat gagaccgcag ctggcacgac aggtttcccg
     6181 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6241 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6301 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg
     6361 actctagagg atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt
     6421 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     6481 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     6541 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     6601 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     6661 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6721 agacaagctg tgacggtctc agggacacga agtgatccgt ttaaactatc agtgtttgac
     6781 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     6841 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgccagccg cctttgcgac
     6901 gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc
     6961 gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct
     7021 cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga
     7081 ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag
     7141 ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat
     7201 gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga
     7261 cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca
     7321 cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg
     7381 cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa
     7441 ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc
     7501 ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc
     7561 gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggccaat tcccgagtgc
     7621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7681 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa
     7741 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     7801 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     7861 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     7921 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     7981 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     8041 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     8101 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     8161 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta
     8221 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     8281 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     8341 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     8401 ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     8461 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc
     8521 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8581 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8641 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8821 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8881 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8941 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9001 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9061 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9121 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9181 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9241 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9301 tacggttcct ggcagatcct agatgtggcg caacgatgcc ggcgacaagc aggagcgcac
     9361 cgacttcttc cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg
     9421 caaggggtcg ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg
     9481 ccagacggtc tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc
     9541 accaggcggg tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc
     9601 gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga
     9661 cgcggggttt tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc
     9721 ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg
     9781 cgacagcgtg caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc
     9841 gcgtcgtctt gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg
     9901 aactatgacg accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga
     9961 ggccaagcag gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc
    10021 cttgttcgat attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg
    10081 ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt
    10141 cattttccac gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga
    10201 cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga
    10261 gccgatcacc ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg
    10321 gtattacacg aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac
    10381 gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga
    10441 ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt
    10501 tgctggcgac cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc
    10561 ccgacggatg ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac
    10621 cttccgcctc atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg
    10681 cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga
    10741 cctggtgcat tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc
    10801 ccctgctcgg atctgttgga ccggacagta gtcatggttg atgggctgcc tgtatcgagt
    10861 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    10921 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    10981 tactggggtt gaacactct
//
