LOCUS       sequence-378301-       13094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the AND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13094)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 13094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP749_L2pV1_AND_B3RT_FRT_Rluc_1IF_lacZ
FEATURES             Location/Qualifiers
     source          1..13094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      878..1585
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1649..1682
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      1683..2390
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    2391..2424
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          2491..2679
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    3707..3881
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             4269..5921
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4335..4353)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5941..6141
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    6484..6539
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6547..6567
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7251..7439
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    8063..8237
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    8307..8328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8343..8348,8349..8366,8367..8373)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8381..8397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8386..8408
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8405..8421
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8405..8421
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8417..8740
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8434..8490
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8491..8508)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8491..8507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8500..8522)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8716..8735)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8872..8896
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8984..9694
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9715..9819
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9820..10614
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10785..11373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11274..11293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11709..12857
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13003..13027
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaaggttgc ttaagaataa gtaattctta agcaaccctg ctttaatgag atatgcgaga
      901 agcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
      961 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     1021 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     1081 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     1141 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     1201 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     1261 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     1321 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     1381 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     1441 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     1501 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     1561 gcgacaacat gtcgaggctc agcagggttg cttaagaata agtaattctt aagcaacctc
     1621 tgtagagtga acttcaccac atattctgga agttcctatt ctctagaaag tataggaact
     1681 tcctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct ttcaattctg
     1741 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
     1801 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
     1861 aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg
     1921 tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt
     1981 ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga
     2041 ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg
     2101 agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt
     2161 gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca
     2221 acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt
     2281 ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc
     2341 gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag gaagttccta
     2401 ttctctagaa agtataggaa cttccaacaa acaacattac aattactatt tacaattaca
     2461 atgacttcaa aggtgtatga tcctgagcag gtaagtttct gcttctacct ttgatatata
     2521 tataataatt atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa
     2581 agaatgtagt atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat
     2641 aacttttcta atatatgacc aaaatttgtt gatgtgcaga gaaaaagaat gataactggt
     2701 ccacaatggt gggcaagatg taaacagatg aacgtgcttg attctttcat taattactac
     2761 gattcagaaa agcacgctga gaatgcagtt atctttcttc acggaaacgc tgcatcttca
     2821 tatttgtgga gacatgttgt gcctcacatt gaaccagtgg ctaggtgtat tatcccagat
     2881 cttatcggaa tgggaaagtc tggtaaatct ggaaatggtt cttacagact tttggatcat
     2941 tataagtacc tcacagcttg gttcgaactt ctcaacttac ctaagaagat tattttcgtt
     3001 ggacacgatt ggggtgcttg cttggcattc cattactcat acgaacacca agataagatc
     3061 aaagctatag ttcatgcaga gagtgttgtg gatgtgatag aatcttggga tgagtggcca
     3121 gatattgaag aggatattgc tttgatcaag tctgaagagg gagaaaagat ggttctcgaa
     3181 aataactttt tcgtggaaac tatgcttcct tcaaagatta tgagaaaatt ggaaccagaa
     3241 gagtttgctg catatctcga acctttcaag gaaaaaggag aggttagaag gccaacactc
     3301 agttggccta gggaaatacc attagttaag ggaggtaaac ctgatgttgt gcagatcgtg
     3361 agaaattata acgcttacct tagggcatca gatgatttgc ctaagatgtt catcgagtca
     3421 gatccaggat ttttcagtaa tgctatagtt gaaggtgcaa agaaatttcc aaacaccgag
     3481 ttcgttaagg tgaaaggatt gcatttctca caagaagatg ctcctgatga gatgggaaag
     3541 tatattaagt cttttgttga aagagtgctc aaaaacgagc agggttctcg atctgatgct
     3601 tctgaagtaa cgtcaacaag ctcccagtct gaggtatgca cagtggaaac gccaggttgt
     3661 gttggttctt aagcttctct agctagagtc gatcgacaag ctcgagtttc tccataataa
     3721 tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg
     3781 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     3841 tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctgcaaga attcaagctt
     3901 ggagccaaca ctcgaatccc cacccgttgg accaaacccg gctcattaag cgtcggttca
     3961 gatttatttc ctttatttaa aaaaaaggaa agggtaaaaa atagaaaatt ggaaacagtt
     4021 aaagcccaaa attgtaattt accgagaatt gtaaatttac ctgaaaaccc tacgctatag
     4081 tttcgactat aaataccaaa cttaggacct cacttcagaa tcccctcgtc gctgcgtctc
     4141 tctcccgcaa ccttcgattt tcgtttattc gcatccatcg gagagagaaa acaatcaata
     4201 agcgacctac tacaaacaag taagtaagag aaaaaccaaa agaagaagag aaacaacaag
     4261 aagaagtaat ggaggacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag
     4321 aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     4381 gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg
     4441 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     4501 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     4561 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     4621 tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga
     4681 acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct aaaacggatt
     4741 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     4801 aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt
     4861 cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca
     4921 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     4981 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     5041 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc
     5101 aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa
     5161 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac
     5221 ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac
     5281 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     5341 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     5401 ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta
     5461 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     5521 tacattctgg agacatagct tactgggacg aggacgaaca cttcttcata gttgaccgct
     5581 tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat
     5641 tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tctgcccgac gatgacgccg
     5701 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     5761 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     5821 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     5881 tcctcataaa ggccaagaag ggcggaaagt ccaaattgta agcttgatcc cccgtcgaca
     5941 gctagctata tcatcaattt atgtattaca cataatatcg cactcagtct ttcatctacg
     6001 gcaatgtacc agctgatata atcagttatt gaaatatttc tgaatttaaa cttgcatcaa
     6061 taaatttatg tttttgcttg gactataata cctgacttgt tattttatca ataaatattt
     6121 aaactatatt tctttcaaga tcgctactag aattcgagct cggagccaac actcgaatcc
     6181 ccacccgttg gaccaaaccc ggctcattaa gcgtcggttc agatttattt cctttattta
     6241 aaaaaaagga aagggtaaaa aatagaaaat tggaaacagt taaagcccaa aattgtaatt
     6301 taccgagaat tgtaaattta cctgaaaacc ctacgctata gtttcgacta taaataccaa
     6361 acttaggacc tcacttcaga atcccctcgt cgctgcgtct ctctcccgca accttcgatt
     6421 ttcgtttatt cgcatccatc ggagagagaa aacaatcaat aagcgaccta ctgtattttt
     6481 acaacaatta ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca
     6541 atggctccta agaagaagag gaaggtgatg agccagttcg acatcctgtg caagaccccc
     6601 cccaaggtgc tggtgcggca gttcgtggag agattcgaga ggcccagcgg cgagaagatc
     6661 gccagctgtg ccgccgagct gacctacctg tgctggatga tcacccacaa cggcaccgcc
     6721 atcaagaggg ccaccttcat gagctacaac accatcatca gcaacagcct gagcttcgac
     6781 atcgtgaaca agagcctgca gttcaagtac aagacccaga aggccaccat cctggaggcc
     6841 agcctgaaga agctgatccc cgcctgggag ttcaccatca tcccttacaa cggccagaag
     6901 caccagagcg acatcaccga catcgtgtcc agcctgcagc tgcagttcga gagcagcgag
     6961 gaggccgaca agggcaacag ccacagcaag aagatgctga aggccctgct gtccgagggc
     7021 gagagcatct gggagatcac cgagaagatc ctgaacagct tcgagtacac cagcaggttc
     7081 accaagacca agaccctgta ccagttcctg ttcctggcca cattcatcaa ctgcggcagg
     7141 ttcagcgaca tcaagaacgt ggaccccaag agcttcaagc tggtgcagaa caagtacctg
     7201 ggcgtgatca ttcagtgcct ggtgaccgag actaagacaa gcgtgtccag gtaagtttct
     7261 gcttctacct ttgatatata tataataatt atcattaatt agtagtaata taatatttca
     7321 aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg tagtttataa
     7381 gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt gatgtgcagg
     7441 cacatctact ttttcagcgc cagaggcagg atcgaccccc tggtgtacct ggacgagttc
     7501 ctgaggaaca gcgagcccgt gctgaagaga gtgaacagga ccggcaacag cagcagcaac
     7561 aagcaggagt accagctgct gaaggacaac ctggtgcgca gctacaacaa ggccctgaag
     7621 aagaacgccc cctaccccat cttcgctatc aagaacggcc ctaagagcca catcggcagg
     7681 cacctgatga ccagctttct gagcatgaag ggcctgaccg agctgacaaa cgtggtgggc
     7741 aactggagcg acaagagggc ctccgccgtg gccaggacca cctacaccca ccagatcacc
     7801 gccatccccg accactactt cgccctggtg tccaggtact acgcctacga ccccatcagc
     7861 aaggagatga tcgccctgaa ggacgagact aaccccatcg aggagtggca gcacatcgag
     7921 cagctgaagg gcagcgccga gggcagcatc agataccccg cctggaacgg catcatcagc
     7981 caggaggtgc tggactacct gagcagctac atcaacaggc ggatctgagc ttctctagct
     8041 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     8101 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     8161 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     8221 gtactaaaat ccagatcgct ttactgagac cgcagctggc acgacaggtt tcccgactgg
     8281 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     8341 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8401 cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactct
     8461 agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
     8521 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     8581 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     8641 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     8701 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     8761 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     8821 agctgtgacg gtctcaggga cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat
     8881 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     8941 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgcc agccgccttt gcgacgctca
     9001 ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
     9061 aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg
     9121 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
     9181 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
     9241 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
     9301 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
     9361 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
     9421 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
     9481 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
     9541 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
     9601 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
     9661 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg ccaattcccg agtgcgcgga
     9721 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9781 ccctgataaa tgcttcaata atattgaaaa aggaagagta tggctaaaat gagaatatca
     9841 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
     9901 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
     9961 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
    10021 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
    10081 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
    10141 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
    10201 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
    10261 aataacgatc tggccgatgt ggattgcgaa aactgggaag aggacactcc atttaaagat
    10321 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
    10381 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
    10441 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
    10501 ctcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
    10561 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagctgtca
    10621 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10681 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10741 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10801 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10861 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10921 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10981 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    11041 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    11101 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    11161 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    11221 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    11281 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11341 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11401 ttcctggcag atcctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact
    11461 tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg
    11521 ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga
    11581 cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag
    11641 gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag
    11701 gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg
    11761 ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg
    11821 aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca
    11881 gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc
    11941 gtcttgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta
    12001 tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca
    12061 agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt
    12121 tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg
    12181 ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt
    12241 tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg
    12301 acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga
    12361 tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt
    12421 acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg
    12481 accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg
    12541 gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg
    12601 gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac
    12661 ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc
    12721 gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag
    12781 cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg
    12841 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcagcccctg
    12901 ctcggatctg ttggaccgga cagtagtcat ggttgatggg ctgcctgtat cgagtggtga
    12961 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
    13021 tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg
    13081 gggttgaaca ctct
//
