LOCUS       sequence-378305-       11582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Off state for the first generation of NOT gates, which target the 
            Rluc CDS to repress expression via its FRT sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 11582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ
FEATURES             Location/Qualifiers
     source          1..11582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          945..1133
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     protein_bind    2127..2160
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2195..2369
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2757..4409
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2823..2841)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4429..4629
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    4972..5027
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5035..5055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5739..5927
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    6551..6725
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    6795..6816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6831..6836,6837..6854,6855..6861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6869..6885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6874..6896
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6893..6909
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6893..6909
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6905..7228
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    6922..6978
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6979..6996)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6979..6995)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6988..7010)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7204..7223)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    7360..7384
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7472..8182
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8203..8307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8308..9102
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9273..9861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9762..9781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10197..11345
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11491..11515
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcca acaaacaaca ttacaattac
      901 tatttacaat tacaatgact tcaaaggtgt atgatcctga gcaggtaagt ttctgcttct
      961 acctttgata tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt
     1021 tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt
     1081 atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg cagagaaaaa
     1141 gaatgataac tggtccacaa tggtgggcaa gatgtaaaca gatgaacgtg cttgattctt
     1201 tcattaatta ctacgattca gaaaagcacg ctgagaatgc agttatcttt cttcacggaa
     1261 acgctgcatc ttcatatttg tggagacatg ttgtgcctca cattgaacca gtggctaggt
     1321 gtattatccc agatcttatc ggaatgggaa agtctggtaa atctggaaat ggttcttaca
     1381 gacttttgga tcattataag tacctcacag cttggttcga acttctcaac ttacctaaga
     1441 agattatttt cgttggacac gattggggtg cttgcttggc attccattac tcatacgaac
     1501 accaagataa gatcaaagct atagttcatg cagagagtgt tgtggatgtg atagaatctt
     1561 gggatgagtg gccagatatt gaagaggata ttgctttgat caagtctgaa gagggagaaa
     1621 agatggttct cgaaaataac tttttcgtgg aaactatgct tccttcaaag attatgagaa
     1681 aattggaacc agaagagttt gctgcatatc tcgaaccttt caaggaaaaa ggagaggtta
     1741 gaaggccaac actcagttgg cctagggaaa taccattagt taagggaggt aaacctgatg
     1801 ttgtgcagat cgtgagaaat tataacgctt accttagggc atcagatgat ttgcctaaga
     1861 tgttcatcga gtcagatcca ggatttttca gtaatgctat agttgaaggt gcaaagaaat
     1921 ttccaaacac cgagttcgtt aaggtgaaag gattgcattt ctcacaagaa gatgctcctg
     1981 atgagatggg aaagtatatt aagtcttttg ttgaaagagt gctcaaaaac gagcagggtt
     2041 ctcgatctga tgcttctgaa gtaacgtcaa caagctccca gtctgaggta tgcacagtgg
     2101 aaacgccagg ttgtgttggt tcttaagaag ttcctattct ctagaaagta taggaacttc
     2161 gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
     2221 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     2281 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2341 aaccaaaatc cagtactaaa atccagatcg ctgcaagaat tcaagcttgg agccaacact
     2401 cgaatcccca cccgttggac caaacccggc tcattaagcg tcggttcaga tttatttcct
     2461 ttatttaaaa aaaaggaaag ggtaaaaaat agaaaattgg aaacagttaa agcccaaaat
     2521 tgtaatttac cgagaattgt aaatttacct gaaaacccta cgctatagtt tcgactataa
     2581 ataccaaact taggacctca cttcagaatc ccctcgtcgc tgcgtctctc tcccgcaacc
     2641 ttcgattttc gtttattcgc atccatcgga gagagaaaac aatcaataag cgacctacta
     2701 caaacaagta agtaagagaa aaaccaaaag aagaagagaa acaacaagaa gaagtaatgg
     2761 aggacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag gatggaaccg
     2821 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     2881 ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc
     2941 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     3001 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     3061 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc
     3121 agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     3181 aattaccaat aatccagaaa attattatca tggattctaa aacggattac cagggatttc
     3241 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     3301 taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc tctggatcta
     3361 ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg
     3421 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     3481 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     3541 tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa
     3601 ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg
     3661 acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag
     3721 aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc
     3781 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     3841 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     3901 tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg
     3961 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     4021 acatagctta ctgggacgag gacgaacact tcttcatagt tgaccgcttg aagtctttaa
     4081 ttaaatacaa aggatatcag gtggcccccg ctgaattgga atcgatattg ttacaacacc
     4141 ccaacatctt cgacgcgggc gtggcaggtc tgcccgacga tgacgccggt gaacttcccg
     4201 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     4261 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     4321 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     4381 ccaagaaggg cggaaagtcc aaattgtaag cttgatcccc cgtcgacagc tagctatatc
     4441 atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc aatgtaccag
     4501 ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata aatttatgtt
     4561 tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa actatatttc
     4621 tttcaagatc gctactagaa ttcgagctcg gagccaacac tcgaatcccc acccgttgga
     4681 ccaaacccgg ctcattaagc gtcggttcag atttatttcc tttatttaaa aaaaaggaaa
     4741 gggtaaaaaa tagaaaattg gaaacagtta aagcccaaaa ttgtaattta ccgagaattg
     4801 taaatttacc tgaaaaccct acgctatagt ttcgactata aataccaaac ttaggacctc
     4861 acttcagaat cccctcgtcg ctgcgtctct ctcccgcaac cttcgatttt cgtttattcg
     4921 catccatcgg agagagaaaa caatcaataa gcgacctact gtatttttac aacaattacc
     4981 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat ggctcctaag
     5041 aagaagagga aggtgatgag ccagttcgac atcctgtgca agaccccccc caaggtgctg
     5101 gtgcggcagt tcgtggagag attcgagagg cccagcggcg agaagatcgc cagctgtgcc
     5161 gccgagctga cctacctgtg ctggatgatc acccacaacg gcaccgccat caagagggcc
     5221 accttcatga gctacaacac catcatcagc aacagcctga gcttcgacat cgtgaacaag
     5281 agcctgcagt tcaagtacaa gacccagaag gccaccatcc tggaggccag cctgaagaag
     5341 ctgatccccg cctgggagtt caccatcatc ccttacaacg gccagaagca ccagagcgac
     5401 atcaccgaca tcgtgtccag cctgcagctg cagttcgaga gcagcgagga ggccgacaag
     5461 ggcaacagcc acagcaagaa gatgctgaag gccctgctgt ccgagggcga gagcatctgg
     5521 gagatcaccg agaagatcct gaacagcttc gagtacacca gcaggttcac caagaccaag
     5581 accctgtacc agttcctgtt cctggccaca ttcatcaact gcggcaggtt cagcgacatc
     5641 aagaacgtgg accccaagag cttcaagctg gtgcagaaca agtacctggg cgtgatcatt
     5701 cagtgcctgg tgaccgagac taagacaagc gtgtccaggt aagtttctgc ttctaccttt
     5761 gatatatata taataattat cattaattag tagtaatata atatttcaaa tatttttttc
     5821 aaaataaaag aatgtagtat atagcaattg cttttctgta gtttataagt gtgtatattt
     5881 taatttataa cttttctaat atatgaccaa aatttgttga tgtgcaggca catctacttt
     5941 ttcagcgcca gaggcaggat cgaccccctg gtgtacctgg acgagttcct gaggaacagc
     6001 gagcccgtgc tgaagagagt gaacaggacc ggcaacagca gcagcaacaa gcaggagtac
     6061 cagctgctga aggacaacct ggtgcgcagc tacaacaagg ccctgaagaa gaacgccccc
     6121 taccccatct tcgctatcaa gaacggccct aagagccaca tcggcaggca cctgatgacc
     6181 agctttctga gcatgaaggg cctgaccgag ctgacaaacg tggtgggcaa ctggagcgac
     6241 aagagggcct ccgccgtggc caggaccacc tacacccacc agatcaccgc catccccgac
     6301 cactacttcg ccctggtgtc caggtactac gcctacgacc ccatcagcaa ggagatgatc
     6361 gccctgaagg acgagactaa ccccatcgag gagtggcagc acatcgagca gctgaagggc
     6421 agcgccgagg gcagcatcag ataccccgcc tggaacggca tcatcagcca ggaggtgctg
     6481 gactacctga gcagctacat caacaggcgg atctgagctt ctctagctag agtcgatcga
     6541 caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga attagggttc
     6601 ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     6661 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
     6721 agatcgcttt actgagaccg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6781 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6841 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6901 agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag aggatccccg
     6961 ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7021 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7081 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     7141 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     7201 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     7261 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgacggt
     7321 ctcagggaca cgaagtgatc cgtttaaact atcagtgttt gacaggatat attggcgggt
     7381 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     7441 tttatccgtt cgtccatttg tatgtgccag ccgcctttgc gacgctcacc gggctggttg
     7501 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
     7561 agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc
     7621 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt
     7681 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa
     7741 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga
     7801 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat
     7861 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg
     7921 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc
     7981 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg
     8041 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc
     8101 tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
     8161 ggcctcaccc caaaaatggc agcgctggcc aattcccgag tgcgcggaac ccctatttgt
     8221 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8281 cttcaataat attgaaaaag gaagagtatg gctaaaatga gaatatcacc ggaattgaaa
     8341 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
     8401 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
     8461 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
     8521 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
     8581 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
     8641 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
     8701 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
     8761 gccgatgtgg attgcgaaaa ctgggaagag gacactccat ttaaagatcc gcgcgagctg
     8821 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     8881 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     8941 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgct cagggaggat
     9001 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     9061 gagaaaataa aatattatat tttactggat gaattgtttt agctgtcaga ccaagtttac
     9121 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9181 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     9421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggcagat
     9901 cctagatgtg gcgcaacgat gccggcgaca agcaggagcg caccgacttc ttccgcatca
     9961 agtgttttgg ctctcaggcc gaggcccacg gcaagtattt gggcaagggg tcgctggtat
    10021 tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg gtctacggga
    10081 ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc gggtcaaatc
    10141 aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga gggtgaatga
    10201 atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg ttttccgccg
    10261 aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa accttccagt
    10321 ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc gtgcaactgg
    10381 ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt cttgaacagg
    10441 aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg acgaccaaga
    10501 agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag caggccgcgt
    10561 tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc gatattgcgc
    10621 cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc ctgttcacca
    10681 cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca
    10741 aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac gaactggtgt
    10801 ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc accttcacgt
    10861 tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac acgaaggccg
    10921 aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac cgcgttgggc
    10981 acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt
    11041 cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca
    11101 cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg atgttcgact
    11161 atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg
    11221 gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt
    11281 tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg cattgcaaac
    11341 gctagggcct tgtggggtca gttccggctg ggggttcagc agcccctgct cggatctgtt
    11401 ggaccggaca gtagtcatgg ttgatgggct gcctgtatcg agtggtgatt ttgtgccgag
    11461 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
    11521 gacgcttaga caacttaata acacattgcg gacgttttta atgtactggg gttgaacact
    11581 ct
//
