LOCUS       sequence-378309-       12155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the on state for testing of the alt VCre recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12155)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP673_L2_pV01_1I_VloxP-OCS-Rluc_TCTP_VCre_lacZ
FEATURES             Location/Qualifiers
     source          1..12155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5680..5735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5740..5760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6200..6388
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     regulatory      6977..6986
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7124..7298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7368..7389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7404..7409,7410..7427,7428..7434)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7442..7458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7447..7469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7466..7482
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7466..7482
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7478..7801
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7495..7551
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7552..7569)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7552..7568)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7561..7583)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7777..7796)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    7933..7957
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8045..8755
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8776..8880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8881..9675
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9846..10434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10335..10354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10770..11918
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12064..12088
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaatcaatt tctgagaact gtcattctcg gaaattgact gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcagtcaa tttctgagaa ctgtcattct cggaaattga
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct
     5401 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
     5461 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
     5521 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
     5581 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
     5641 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
     5701 caacaaacaa cattacaatt actatttaca attacaatgc ccaagaaaaa gcggaaagtg
     5761 atcgagaacc agctgagcct gctgggcgac ttttctggcg tgcggcccga cgatgtgaaa
     5821 accgccattc aggccgccca gaaaaagggc atcaacgtgg ccgagaacga gcagttcaag
     5881 gccgccttcg agcatctgct gaacgagttc aagaagcggg aagagagata cagccccaac
     5941 accctgcggc ggctggaaag cgcctggacc tgcttcgtgg attggtgcct ggccaaccac
     6001 agacacagcc tgcctgccac ccccgatacc gtggaagcct tcttcatcga gcgggccgag
     6061 gaactgcacc ggaacaccct gagcgtgtac agatgggcca tcagccgggt gcacagagtg
     6121 gccggatgcc ctgatccctg cctggacatc tacgtggaag atcggctgaa ggccattgcc
     6181 cggaagaaag tgcgggaagg taagtttctg cttctacctt tgatatatat ataataatta
     6241 tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta
     6301 tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa
     6361 tatatgacca aaatttgttg atgtgcaggt gaggccgtga agcaggccag ccctttcaac
     6421 gagcagcatc tgctgaagct gaccagcctg tggtacagaa gcgacaagct gctgctgcgg
     6481 cggaacctgg ctctgctggc tgtggcctac gagagcatgc tgagagccag cgagctggcc
     6541 aacatccggg tgtccgatat ggaactggcc ggcgacggaa ccgccatcct gaccatccct
     6601 atcaccaaga ccaaccactc cggcgagccc gatacctgca tcctgtccca ggatgtggtg
     6661 tccctgctga tggactacac cgaggccggc aagctggata tgagcagcga cggcttcctg
     6721 ttcgtgggcg tgtccaagca caacacctgt atcaagccca agaaggacaa gcagaccggc
     6781 gaggtgctgc acaagcccat caccaccaag acagtggaag gcgtgttcta cagcgcctgg
     6841 gagacactgg acctgggcag acagggcgtg aagcctttca cagcccacag cgccagagtg
     6901 ggagccgctc aggacctgct gaagaagggc tacaataccc tgcagatcca gcagtccggc
     6961 cggtggtcta gcggagccat ggtggccaga tacggcagag ccatcctggc tagggatggc
     7021 gctatggccc acagcagagt gaaaaccaga tccgccccca tgcagtgggg caaggacgag
     7081 aaggactgag cttctctagc tagagtcgat cgacaagctc gagtttctcc ataataatgt
     7141 gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc
     7201 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     7261 aattcctaaa accaaaatcc agtactaaaa tccagatcgc tttactgaga ccgcagctgg
     7321 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7381 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7441 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
     7501 gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cactggccgt
     7561 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     7621 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7681 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     7741 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     7801 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7861 cccggcatcc gcttacagac aagctgtgac ggtctcaggg acacgaagtg atccgtttaa
     7921 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     7981 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     8041 cagccgcctt tgcgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
     8101 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc
     8161 ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg
     8221 acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc
     8281 ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga
     8341 gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga
     8401 ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg
     8461 acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca
     8521 tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta
     8581 aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca
     8641 cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca
     8701 tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg
     8761 gccaattccc gagtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8821 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8881 atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta
     8941 aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac
     9001 ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa
     9061 aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa
     9121 cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa
     9181 gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg
     9241 ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta
     9301 gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa
     9361 gaggacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc
     9421 gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat
     9481 ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac
     9541 attgccttct gcgtccggtc gctcagggag gatatcgggg aagaacagta tgtcgagcta
     9601 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg
     9661 gatgaattgt tttagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9721 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9781 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9841 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9901 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9961 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10021 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10081 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10141 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10201 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10261 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10321 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10381 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10441 agcaacgcgg cctttttacg gttcctggca gatcctagat gtggcgcaac gatgccggcg
    10501 acaagcagga gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc
    10561 acggcaagta tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca
    10621 agtacgagaa ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt
    10681 atctggacac caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt
    10741 gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca
    10801 ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca
    10861 ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta
    10921 cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg
    10981 ccgccgtgga gcgttcgcgt cgtcttgaac aggaggcggc aggtttggcg aagtcgatga
    11041 ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg
    11101 caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca
    11161 aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc
    11221 caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg
    11281 cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg
    11341 tcgagctgcg ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc
    11401 gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct
    11461 ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga
    11521 cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc
    11581 gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg
    11641 aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca
    11701 agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc
    11761 cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt
    11821 ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg
    11881 cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg
    11941 ctgggggttc agcagcccct gctcggatct gttggaccgg acagtagtca tggttgatgg
    12001 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
    12061 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
    12121 gcggacgttt ttaatgtact ggggttgaac actct
//
