LOCUS       sequence-378326-        4641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains homology arms for the oscillin (NIES39_A01430) locus of 
            Arthrospira platensis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Jacob M. Hilzinger, Skyler Friedline, Divya Sivanandan, Ya-Fang 
            Cheng, Shunsuke Yamazaki, Douglas S. Clark, Jeffrey M. Skerker, Adam
            P. Arkin
  TITLE     Acetaminophen production in the edible, filamentous cyanobacterium 
            Arthrospira platensis
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: pJMH022
FEATURES             Location/Qualifiers
     source          1..4641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2420..2436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2420..2436)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2433..2455)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2444..2460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2468..2474,2475..2492,2493..2498))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2513..2534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2651..2668)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2822..3410)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2902..2921)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3581..4372,4373..4441))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4204..4223
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4442..4546)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4614..4632
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgtgct acggtaaatc cttacccaat
      421 ggcatgagtt acaaagacaa aatgctggct cagatccccc tggatctatc caggattcac
      481 tttgtcggtc cgttgcccta tggacaatac ctgaaagtga ttcaagcctc tgcagtccat
      541 gtatacttaa ctcgaccctt tgtattgtcc tggtctatga tagaaagcct ttccaccggt
      601 tgtctagtca ttggttcgga caccccccct attcgggagg tcattcaaga tggctataac
      661 ggcttactgg tcgatttctt ttctcccaaa caaatcgccg accgggttga tgaggtatta
      721 gaccacccca accgcatggc agatttgcga gtccgagcca gggaaacagt tatagagcgt
      781 tatgatctgt ctaaattgtt gtcaaaacaa ctgcaactga ttgaacaagt cgctcaaaag
      841 tcccggccta cctctgggga acagatttcc tcccagcccg tcattgcata ggcgatcgag
      901 gtttccctaa cccgaaactt tgaccccaaa ttatctaatt tggcgcagcc aagttggtgg
      961 tcataaataa catataattc ccatcaaata gacctttgag ctagacgaaa aaaggaggag
     1021 ttcccggcaa caaaatcaaa tctgagccaa atcttatccg atgcttataa cgcctttttt
     1081 ggctgaaact ggctgaaata aggggtgggc aggaacatag tagggatata tttcgtccca
     1141 aagcacaaga gtacccaaga taatctttaa gagtaatgat tacaagacaa ttctggagaa
     1201 attgatccaa gaaccaatca taatacaccc aagcaatcct aaccacacta cagacattgt
     1261 agacagaaca gtggggaagg gactagactt gctgagggta aaaaggttaa actactgcgg
     1321 aacaatctct ggtgtttcag gcagtgtctg tagtcccaac ggatcaaaaa acatcattgc
     1381 ggaggagtga aacaaatcta gatgacatag gggttaagga gcgataggcg attggggttg
     1441 gcgtaagcta ccaaaagcga tcgcttctat ccctgaaaaa tgccagacct caatcttgtc
     1501 aattttcccc cgaatagggg gaattttatt ttgatttaag aattacttgg tcattggtga
     1561 gctacaatcc gggaatgctt aatctaactt acgagtgtaa attatggcat gacctatagc
     1621 ctcagaattg cggatatccc taccagtgag agaccccgtg aaagattaat ggctttcgga
     1681 ccccaaagcc tctctacagc agaattaata gcgattttgt tgggaacagg tcagggagcc
     1741 gggaaactct cgtcggttgg tctgggacaa tacatattaa aggaactcag ccagtgtgag
     1801 gaagatcccc tttcggtgat gcgaaatgtg ggggttgggg aacttactaa aattcacgga
     1861 gttggtccgg ctaaggctac tactatcata gctgcgatcg aattgggaaa gcgagtcttt
     1921 ctatctaaac ccccggaatt gacggtcatt gacagccccc aagcggctgc taatgctctc
     1981 agccataatt tgatgtggca gacacaagaa agatttgcta tcttgttaat ggatgtgaaa
     2041 aaccgcctat tgggaactaa gctgttgacc ataggaacgg ctacggaaac gctggctcat
     2101 ccccgtgaaa ttttccggga ggtactcaaa cacaatgcca cccgtctgat tattgctcat
     2161 aatcacccat caggaaatgt tgaacctagt catcaagata tcgagctgac ccgccaactt
     2221 ctcgaagggg gtcaattctt gtctttacct atccttgacc acctgatatt aggtaacgga
     2281 gattatggga gtattcgcca aatgacaaaa ttatgggaag aataccccca aggagaatag
     2341 ggaattattt gtcggaaatg gggactgatt cattgatcag taaaccgacg gggaaatact
     2401 ggagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2461 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2521 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2581 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2641 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2701 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2761 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2821 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2881 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2941 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3001 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3061 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3121 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3181 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3241 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3301 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3361 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3421 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3481 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3541 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3661 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3721 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3781 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3841 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3901 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3961 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4021 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4081 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4141 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4201 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4261 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4321 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4381 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4441 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4501 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4561 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4621 gtatcacgag gccctttcgt c
//
