LOCUS       sequence-378329-       11648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 
            3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Liu Y, Pinto F, Wan X, Yang Z, Peng S, Li M, Cooper JM, Xie Z, 
            French CE, Wang B
  TITLE     Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and 
            sequence-specific RNA biosensors.
  JOURNAL   Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x.
  PUBMED    35410423
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-04-11"; volume: "13(1):1937. doi"; pages: " 
            10.1038/s41467-022-29604-x"
COMMENT     Sequence Label: pLY164
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(113..949)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        complement(967..1001)
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      1017..1044
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1102..1220
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             2218..2229
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_RNA        2432..2481
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             2514..2525
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2532..3245
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    3352..3372
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3373..3430
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3454..3471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(3733..4683)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4731..4953
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5445..5539)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5569..6360,6361..6429))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6192..6211
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6430..6534)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6599..6617
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(6621..6664)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    6667..6688
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(join(6752..8338,8339..8341,8342..10828,
                     10829..10831,10832..10858))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             10865..10876
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        10894..10949
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    10895..10913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             10965..11591
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(11041..11060)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             11606..11617
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tactagagcc tagttgtctt catgcatgaa gacaaaatta atactagagg gaccaaaacg
       61 aaaaaagacg cttttcagcg tcttattgtt cgtctttggt accgagctag tattattgca
      121 gaaagccatc ccgtccctgg cgaatatcac gcggtgacca gttaaactct cggcgaaaaa
      181 gcgtcgaaaa gtggttactg tcgctgaatc cacagcgata ggcgatgtca gtaacgctgg
      241 cctcgctgtg gcgtagcaga tgtcgggctt tcatcagtcg caggcggttc aggtatcgct
      301 gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt gaaagagaaa
      361 attgatccgc cacggcatcc caattcacct catcggcaaa atggtcctcc agccaggcca
      421 gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg cttttacgca
      481 gcaagagcag taattgcata aacaagatct cgcgactggc ggtcgagggt aaatcatttt
      541 ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat acgctgtggt
      601 taacgcgcca gtgagacgga tactgcccat ccagctcttg tggcagcaac tgattcagcc
      661 cggcgagaaa ctgaaatcga tccggcgagc gatacagcac attggtcaga cacagattat
      721 cggtatgttc atacagatgc cgatcatgat cgcgtacgaa acagaccgtg ccaccggtga
      781 tggtataggg ctgcccatta aacacatgaa tacccgtgcc atgttcgaca atcacaattt
      841 catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt tctatcgcca
      901 cggacgcgtt accggacgga aaaaaatcca cactatgtaa tacggtcatt tgggctccct
      961 ctagtagcta gcataatacc taggactgag ctagctgtaa actctagtat cacactggct
     1021 caccttcggg tgggcctttc tgcgtttata tactagagag acctttacgc cgctggagca
     1081 ggaatgcggt gagcatcaca tcaccacaat tcagcaaatt gtgaacatca tcacgttcat
     1141 ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtcg cgaattgagg
     1201 cgctttttag actggtcgta actagagtca cacaggaaag tactagatgg cagaatacaa
     1261 agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg tttcgcatct
     1321 cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta aagagctgat
     1381 tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt cccttaactg
     1441 cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag cgggggcgtt
     1501 taccggtgcg caaaaacgtc atccagggag atttgaacgt gccgacggcg gtacgctatt
     1561 tcttgatgaa ctcgctacgg caccgatgat ggtgcaggag aaattattgc gcgtgattga
     1621 gtacggtgaa ctggagcgcg ttggcggcag ccaaccattg caggtgaatg tgcggttggt
     1681 atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggcacttttc gcgctgacct
     1741 gctcgaccga ctggcttttg atgttgtaca actgccacca ctgcgcgagc gcgaaagcga
     1801 cataatgttg atggcagaat actttgccat ccagatgtgt cgggaaatca agctgcctct
     1861 gttcccgggg tttacggagc gcgccagaga aacattgctg aattatcgtt ggccgggaaa
     1921 tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca ccagcgatta
     1981 tccgcttgat gacatcatta ttgatccctt taaacggcgt ccgcctgaag acgctatcgc
     2041 cgtttcagaa accacctcgc ttccaacact gccgctggat ttacgtgagt ttcagatgca
     2101 gcaggaaaaa gagttgctgc aactcagttt gcaaatgaat gcacgcacac gccgccgcga
     2161 acgtcgcgca gagaaacagg ctcaatggaa agcagcaaat taatactaga gccaattatt
     2221 gaaggccgct aacgcggcct ttttttgttt ctggtctccc tactagagtg aaggacggcg
     2281 gccactaccg gttagtgtaa ttcgctaact catcctggca tgttgctgtt gattcttcaa
     2341 tcagatcttt ataaatcaaa aagataaaaa attggcacgc aaattgtatt aacagttcag
     2401 caggacaatc ctgaacgcaa atactagaga gctgtcaccg gatgtgcttt ccggtctgat
     2461 gagtccgtga ggacgaaaca gcctctacaa ataattttgt ttaactagag attaaagagg
     2521 agaaatacca tatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg
     2581 aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg
     2641 caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt
     2701 ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc
     2761 atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca
     2821 cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg
     2881 ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc
     2941 tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac
     3001 aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc
     3061 agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag
     3121 acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc
     3181 atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt
     3241 acaaaaggcc tgctgcaaac gacgaaaact acgctgcatc agtttaataa tactagagcc
     3301 aattattgaa ggcctcccta acggggggcc tttttttgtt tctggtctcc ctactagtag
     3361 cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
     3421 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
     3481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatctcga ggttacattg
     3541 tcgatctgtt catggtgaac agctttgaat gcaccaaaaa ctcgtaaaag ctctgatgta
     3601 tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatgtaa
     3661 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     3721 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     3781 tttttgcgtg agccatgaga acgaaccatt gagatcatac ttactttgca tgtcactcaa
     3841 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     3901 tcttagtccg ttatgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     3961 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     4021 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     4081 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     4141 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     4201 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     4261 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     4321 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     4381 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     4441 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     4501 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     4561 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     4621 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     4681 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     4741 atgataatta catgtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt
     4801 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     4861 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     4921 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     4981 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     5041 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca
     5101 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
     5161 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
     5221 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat
     5281 gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac
     5341 cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg
     5401 gtatcatcaa caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa
     5461 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     5521 ggatctatca acaggagtcc aagcgagctc gtaaacttgg tctgacagtt accaatgctt
     5581 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5641 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5701 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5761 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5821 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5881 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5941 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6001 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6061 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6121 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6181 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6241 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6301 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6361 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6421 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6481 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6541 tcccatggtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6601 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
     6661 tttctggaat tcgcggccgc ttctagaggg gagaccagaa acaaaaaaag gggagcggtt
     6721 tcccgctccc cttcaataat tggctctagt attagtcacc tcctagctga ctcaaatcaa
     6781 tgcgtgtttc ataaagacca gtgatggatt gatggataag agtggcatct aaaacttctt
     6841 ttgtagacgt atatcgttta cgatcaattg ttgtatcaaa atatttaaaa gcagcgggag
     6901 ctccaagatt cgtcaacgta aataaatgaa taatattttc tgcttgttca cgtattggtt
     6961 tgtctctatg tttgttatat gcactaagaa ctttatctaa attggcatct gctaaaataa
     7021 cacgcttaga aaattcactg atttgctcaa taatctcatc taaataatgc ttatgctgct
     7081 ccacaaacaa ttgtttttgt tcgttatctt ctggactacc cttcaacttt tcataatgac
     7141 tagctaaata taaaaaattc acatatttgc ttggcagagc cagctcattt cctttttgta
     7201 attctccggc actagccagc atccgtttac gaccgttttc taactcaaaa agactatatt
     7261 taggtagttt aatgattaag tcttttttaa cttccttata tcctttagct tctaaaaagt
     7321 caatcggatt tttttcaaag gaacttcttt ccataattgt gatccctagt aactctttaa
     7381 cggattttaa cttcttcgat ttcccttttt ccaccttagc aaccactagg actgaataag
     7441 ctaccgttgg actatcaaaa ccaccatatt tttttggatc ccagtctttt ttacgagcaa
     7501 taagcttgtc cgaatttctt tttggtaaaa ttgactcctt ggagaatccg cctgtctgta
     7561 cttctgtttt cttgacaata ttgacttggg gcatggacaa tactttgcgc actgtggcaa
     7621 aatctcgccc tttatcccag acaatttctc cagtttcccc attagtttcg attagagggc
     7681 gtttgcgaat ctctccattt gcaagtgtaa tttctgtttt gaagaagttc atgatattag
     7741 agtaaaagaa atattttgcg gttgctttgc ctatttcttg ctcagactta gcaatcattt
     7801 tacgaacatc ataaacttta taatcaccat agacaaactc cgattcaagt tttggatatt
     7861 tcttaatcaa agcagttcca acgacggcat ttagatacgc atcatgggca tgatggtaat
     7921 tgttaatctc acgtacttta tagaattgga aatcttttcg gaagtcagaa actaatttag
     7981 attttaaggt aatcacttta acctctcgaa taagtttatc attttcatcg tatttagtat
     8041 tcatgcgact atccaaaatt tgtgccacat gcttagtgat ttggcgagtt tcaaccaatt
     8101 ggcgtttgat aaaaccagct ttatcaagtt cactcaaacc tccacgttca gctttcgtta
     8161 aattatcaaa cttacgttga gtgattaact tggcgtttag aagttgtctc caatagtttt
     8221 tcatcttttt gactacttct tcacttggaa cgttatccga tttaccacga tttttatcag
     8281 aacgcgttaa gaccttattg tctattgaat cgtctttaag gaaactttgt ggaacaatgg
     8341 catcgacatc ataatcactt aaacgattaa tatctaattc ttggtccaca tacatgtctc
     8401 ttccattttg gagataatag agatagagct tttcattttg caattgagta ttttcaacag
     8461 gatgctcttt aagaatctga cttcctaatt ctttgatacc ttcttcgatt cgtttcatac
     8521 gctctcgcga atttttctgg cccttttgag ttgtctgatt ttcacgtgcc atttcaataa
     8581 cgatattttc tggcttatgc cgccccatta ctttgaccaa ttcatcaaca acttttacag
     8641 tctgtaaaat acctttttta atagcagggc taccagctaa atttgcaata tgttcatgta
     8701 aactatcgcc ttgtccagac acttgtgctt tttgaatgtc ttctttaaat gtcaaactat
     8761 catcatggat cagctgcata aaattgcgat tggcaaaacc atctgatttc aaaaaatcta
     8821 atattgtttt gccagattgc ttatccctaa taccattaat caattttcga gacaaacgtc
     8881 cccaaccagt ataacggcga cgtttaagct gtttcatcac cttatcatca aagaggtgag
     8941 catatgtttt aagtctttcc tcaatcatct ccctatcttc aaataaggtc aatgttaaaa
     9001 caatatcctc taagatatct tcattttctt cattatccaa aaaatcttta tctttaataa
     9061 tttttagcaa atcatggtag gtacctaatg aagcattaaa tctatcttca actcctgaaa
     9121 tttcaacact atcaaaacat tctatttttt tgaaataatc ttcttttaat tgcttaacgg
     9181 ttacttttcg atttgttttg aagagtaaat caacaatggc tttcttctgt tcacctgaaa
     9241 gaaatgctgg ttttcgcatt ccttcagtaa catatttgac ctttgtcaat tcgttataaa
     9301 ccgtaaaata ctcataaagc aaactatgtt ttggtagtac tttttcattt ggaagatttt
     9361 tatcaaagtt tgtcatgcgt tcaataaatg attgagctga agcaccttta tcgacaactt
     9421 cttcaaaatt ccatggggta attgtttctt cagacttccg agtcatccat gcaaaacgac
     9481 tattgccacg cgccaatgga ccaacataat aaggaatacg aaaagtcaag attttttcaa
     9541 tcttctcacg attgtctttt aaaaatggat aaaagtcttc ttgtcttctc aaaatagcat
     9601 gcagctcacc caagtgaatt tgatggggaa tagagccgtt gtcaaaggtc cgttgcttgc
     9661 gcagcaaatc ttcacgattt agtttcacca ataattcctc agtaccatcc attttttcta
     9721 aaattggttt gataaattta taaaattctt cttggctagc tcccccatca atataacctg
     9781 catatccgtt ttttgattga tcaaaaaaga tttctttata cttttctgga agttgttgtc
     9841 gaactaaagc ttttaaaaga gtcaagtctt gatgatgttc atcgtagcgt ttaatcattg
     9901 aagctgatag gggagcctta gttatttcag tatttactct taggatatct gaaagtaaaa
     9961 tagcatctga taaattctta gctgccaaaa acaaatcagc atattgatct ccaatttgcg
    10021 ccaataaatt atctaaatca tcatcgtaag tatcttttga aagctgtaat ttagcatctt
    10081 ctgccaaatc aaaatttgat ttaaaattag gggtcaaacc caatgacaaa gcaatgagat
    10141 tcccaaataa gccatttttc ttctcaccgg ggagctgagc aatgagattt tctaatcgtc
    10201 ttgatttact caatcgtgca gaaagaatcg ctttagcatc tactccactt gcgttaatag
    10261 ggttttcttc aaataattga ttgtaggttt gtaccaactg gataaatagt ttgtccacat
    10321 cactattatc aggatttaaa tctccctcaa tcaaaaaatg accacgaaac ttaatcatat
    10381 gcgctaaggc caaatagatt aagcgcaaat ccgctttatc agtagaatct accaattttt
    10441 ttcgcagatg atagatagtt ggatatttct catgataagc aacttcatct actatatttc
    10501 caaaaatagg atgacgttca tgcttcttgt cttcttccac caaaaaagac tcttcaagtc
    10561 gatgaaagaa actatcatct actttcgcca tctcatttga aaaaatctcc tgtagataac
    10621 aaatacgatt cttccgacgt gtataccttc tacgagctgt ccgtttgagg cgagtcgctt
    10681 ccgctgtctc tccactgtca aataaaagag cccctataag attttttttg atactgtggc
    10741 ggtctgtatt tcccagaacc ttgaactttt tagacggaac cttatattca tcagtgatca
    10801 ccgcccatcc gacgctattt gtgccgatag ctaagcctat tgagtatttc ttatccatag
    10861 atcctttctc ctctttagat cttttcaatt cttttctcta tcactgatag ggagtggtaa
    10921 aataactcta tcaacgatag agtgtcaaca aaaattagga attaatgatg tcaagattag
    10981 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
    11041 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
    11101 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
    11161 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
    11221 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
    11281 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
    11341 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
    11401 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
    11461 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
    11521 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
    11581 gtgggtctta atactagagc caattattga aggccgctaa cgcggccttt ttttgtttct
    11641 ggtctccc
//
