LOCUS       sequence-378339-       11709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Target-AID3S in yeasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Li A, Mitsunobu H, Yoshioka S, Suzuki T, Kondo A, Nishida K
  TITLE     Cytosine base editing systems with minimized off-target effect and 
            molecular size.
  JOURNAL   Nat Commun. 2022 Aug 8;13(1):4531. doi: 10.1038/s41467-022-32157-8.
  PUBMED    35941130
REFERENCE   2  (bases 1 to 11709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-08-8"; volume: "13(1):4531. doi"; pages: " 
            10.1038/s41467-022-32157-8"
COMMENT     Sequence Label: Yeast_Target-AID3S
FEATURES             Location/Qualifiers
     source          1..11709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        610..714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(715..783,784..1575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(933..952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1746..2334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2235..2254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2488..2505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2622..2643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2658..2663,2664..2681,2682..2688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2701..2723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2720..2736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2852..3528
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    3080..3197
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3492..3515
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             6118..6129
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8043..8063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8070..8324
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     terminator      8337..8584
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(8353..8371)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(8602..8621)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8603..8621)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8631..8648)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8631..8647)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8640..8662)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8788..9243
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8875..8894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9085..9106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9544..9951
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             9952..11046
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11540..11559)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11659..11681
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcgaaatt
     2761 aaccctcact aaagggaaca aaagctggta ccgggcccga attctctcct tctcttaggt
     2821 ggcagagcag gtggagggtc gaccatacta gtttcaaaaa ttcttacttt ttttttggat
     2881 ggacgcaaag aagtttaata atcatattac atggcattac caccatatac atatccatat
     2941 acatatccat atacatatcc atatctaatc ttacttatat gttgtggaaa tgtaaagagc
     3001 cccattatct tagcctaaaa aaaccttctc tttggaactt tcagtaatac gcttaactgc
     3061 tcattgctat attgaagtac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga
     3121 agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct
     3181 cgcgccgcac tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga
     3241 ggaaaaattg gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac
     3301 cataggatga taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag
     3361 cgatgatttt tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact
     3421 aatactttca acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac
     3481 aaaaaattgt taatatacct ctatacttta acgtcaagga gaaaaaaccc atggacaaga
     3541 agtactccat tgggctcgct atcggcacaa acagcgtcgg ttgggccgtc attacggacg
     3601 agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc cacagcataa
     3661 agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa gccacgcggc
     3721 tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc tacctgcagg
     3781 agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg ctggaggagt
     3841 cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc aatatcgtgg
     3901 acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag aagcttgtag
     3961 acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat atgatcaaat
     4021 ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat gtcgacaaac
     4081 tctttatcca actggttcag acttacaatc agcttttcga agagaacccg atcaacgcat
     4141 ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg cggctcgaaa
     4201 acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat cttatcgccc
     4261 tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa gatgccaagc
     4321 ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc cagatcggcg
     4381 accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt ctgctgagtg
     4441 atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt atgatcaagc
     4501 gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga cagcaactgc
     4561 ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc ggatacattg
     4621 acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg gaaaaaatgg
     4681 acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc aaacagcgca
     4741 ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac gctatcctca
     4801 ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt gagaaaatcc
     4861 tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc agattcgcgt
     4921 ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa gtcgtggata
     4981 agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa aatctgccta
     5041 acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt tataacgagc
     5101 tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg tctggagagc
     5161 agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc gtgaaacagc
     5221 tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc agcggagtgg
     5281 aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc attaaagaca
     5341 aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc ctcaccctta
     5401 cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct catctcttcg
     5461 acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg cggctgtcaa
     5521 gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg gattttctta
     5581 agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac tctctcacct
     5641 ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt cacgagcaca
     5701 tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc gttaaggtcg
     5761 tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt atcgagatgg
     5821 cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg atgaagagga
     5881 ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca gttgaaaaca
     5941 cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg gacatgtacg
     6001 tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat atcgtgcccc
     6061 agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc gataaaaata
     6121 gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa aattattggc
     6181 ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg actaaggctg
     6241 aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag cttgttgaga
     6301 cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac accaagtacg
     6361 atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct aagctggtct
     6421 cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat taccaccatg
     6481 cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa tatcccaagc
     6541 ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa atgatcgcaa
     6601 agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc aatattatga
     6661 attttttcaa gaccgagatt acactggcca attcgcatag atgctacgtt ctctttgaat
     6721 taaaacgacg gggtgaacgt agagcgtgtt tttggggcta tgctgtgaat aaaccacaga
     6781 gcgggacaga acgtggcatt cacgccgaaa tctttagcat tagaaaagtc gaagaatacc
     6841 tgcgcgacaa ccccggacaa ttcacgataa attggtactc atcctggagt ccttgtgcag
     6901 attgcgctga aaagatctta gaatggtata accaggagct gcgggggaac ggccacactt
     6961 tgaaaatcga agcttgcaaa ctctattacg agaaaaatgc gaggaatcaa attgggctgc
     7021 aaaacctcag agataacggg gttgggttga atgtaggcgg aggcggtagc gacgtcggtg
     7081 gaggttcagg agagattcgg aagcgaccac ttatcgaaac aaacggagaa acaggagaaa
     7141 tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg
     7201 tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa agtatcctcc
     7261 cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg
     7321 gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag
     7381 ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc atggagcgat
     7441 caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa
     7501 aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac ggccggaaac
     7561 gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg ccctctaaat
     7621 acgttaattt cttgtatctg gccagccact atgaaaagct caaagggtct cccgaagata
     7681 atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag atcatcgagc
     7741 aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat aaggtgcttt
     7801 ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac attatccact
     7861 tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac accaccatag
     7921 acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt catcagtcaa
     7981 ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac agcagggctg
     8041 accccaagaa gaagaggaag gtgggtacca tgaccaacct ttccgacatc atagagaagg
     8101 aaacaggcaa acagttggtc atccaagagt cgatactcat gcttcctgaa gaagttgagg
     8161 aggtcattgg gaataagccg gaaagtgaca ttctcgtaca cactgcgtat gatgagagca
     8221 ccgatgagaa cgtgatgctg ctcacgtcag atgccccaga gtacaaaccc tgggctctgg
     8281 tgattcagga ctctaatgga gagaacaaga tcaagatgct atgatctctt ctcgagtcat
     8341 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
     8401 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
     8461 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
     8521 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
     8581 ttgcggccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     8641 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     8701 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     8761 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     8821 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8881 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8941 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     9001 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9061 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9121 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9181 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     9241 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatcgac
     9301 cggtcgagga gaacttctag tatatctaca tacctaatat tattgcctta ttaaaaatgg
     9361 aatcccaaca attacatcaa aatccacatt ctcttcaaaa tcaattgtcc tgtacttcct
     9421 tgttcatgtg tgttcaaaaa cgttatattt ataggataat tatactctat ttctcaacaa
     9481 gtaattggtt gtttggccga gcggtctaag gcgcctgatt caagaaatat cttgaccgca
     9541 gttaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca
     9601 ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc
     9661 gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact
     9721 gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca tatagaatag
     9781 agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat attatttaag
     9841 gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac ttttcttacc
     9901 ttttacattt cagcaatata tatatatata tttcaaggat ataccattct aatgtctgcc
     9961 cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc
    10021 attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat
    10081 catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga tgaggcgctg
    10141 gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc taaatggggt
    10201 accggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact tcaattgtac
    10261 gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc tccaatcaag
    10321 ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg aggtatttac
    10381 tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga acaatacacc
    10441 gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca acatgagcca
    10501 ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag attatggaga
    10561 aaaactgtgg aggaaaccat caagaacgag ttccctacat tgaaggttca acatcaattg
    10621 attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg tattataatc
    10681 accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc aggttccttg
    10741 ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc atttggtttg
    10801 tacgaaccat gccacggttc tgctccagat ttgccaaaga ataaggtcaa ccctatcgcc
    10861 actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga agaaggtaag
    10921 gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg tgatttaggt
    10981 ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt
    11041 gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa tgaaattcat
    11101 aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg aaactatata
    11161 cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga atacaggtaa
    11221 gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt aacattaata
    11281 ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg
    11341 attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa
    11401 acactcaatg acctgaccat ttgatggagt ttaagtcaat accttcttga accatttccc
    11461 ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta acgacgtagt
    11521 cgatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
    11581 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
    11641 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
    11701 aacgcgcga
//
