LOCUS       sequence-378340-       10385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SaAID in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Li A, Mitsunobu H, Yoshioka S, Suzuki T, Kondo A, Nishida K
  TITLE     Cytosine base editing systems with minimized off-target effect and 
            molecular size.
  JOURNAL   Nat Commun. 2022 Aug 8;13(1):4531. doi: 10.1038/s41467-022-32157-8.
  PUBMED    35941130
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-08-8"; volume: "13(1):4531. doi"; pages: " 
            10.1038/s41467-022-32157-8"
COMMENT     Sequence Label: Mammalian_SaAID
FEATURES             Location/Qualifiers
     source          1..10385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..155
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     147..164
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     148..164
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        174..192
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     352..371
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        461..536
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     enhancer        551..930
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        931..1134
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1085..1105
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1179..1197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1226..1246
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4427..4447
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4655..4720
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4727..5350
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             5354..5374
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5381..5635
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     polyA_signal    5649..5770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5686..5705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5740..5759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5807..5827)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5807..5825)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(5876..6464)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5956..5975)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6635..7426,7427..7495))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7258..7277
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7496..7600)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7763..7784)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7995..9173
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8226..9164
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9121..9141
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      9194..9203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9200..10135
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQSCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     polyA_signal    join(10165..10385,1..4)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     join(10378..10385,1..10)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 acagtcgagg ctgatcattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
       61 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      121 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
      181 actcactata gggcgaattg ggtaccgggc caattcgaac gctgacgtca tcaacccgct
      241 ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt gcgccctggc
      301 aggaagatgg ctgtgaggga caggggagtg gcgccctgca atatttgcat gtcgctatgt
      361 gttctgggaa atcaccataa acgtgaaatg tctttggatt tgggaatctt ataagttctg
      421 tatgaggacc acagatcccc gatgttccaa tcagtacgca gttttagtac tctggaaaca
      481 gaatctacta aaacaaggca aaatgccgtg tttatctcgt caacttgttg gcgagatttt
      541 tttacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      601 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      661 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      721 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      781 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      841 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      901 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      961 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     1021 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     1081 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
     1141 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
     1201 acccaagctg gctagcgaaa tggcaccgaa gaagaagcgt aaagtcggaa tccacggagt
     1261 tcctgcggca aagcgaaatt acattctggg gctggccatt ggcattacat cagtgggcta
     1321 tggcatcatt gactacgaga caagggacgt gatcgacgcc ggcgtgagac tgttcaagga
     1381 ggccaacgtg gagaacaatg agggccggag atccaagagg ggagcaaggc gcctgaagcg
     1441 gagaaggcgc cacagaatcc agagagtgaa gaagctgctg ttcgattaca acctgctgac
     1501 cgaccactcc gagctgtctg gcatcaatcc ttatgaggcc agagtgaagg gcctgtccca
     1561 gaagctgtct gaggaggagt ttagcgccgc cctgctgcac ctggcaaaga ggagaggcgt
     1621 gcacaacgtg aatgaggtgg aggaggacac cggcaacgag ctgtccacaa aggagcagat
     1681 cagccgcaat tccaaggccc tggaggagaa gtatgtggcc gagctgcagc tggagcggct
     1741 gaagaaggat ggcgaggtga ggggctccat caatcgcttc aagacctctg actacgtgaa
     1801 ggaggccaag cagctgctga aggtgcagaa ggcctaccac cagctggatc agtcctttat
     1861 cgatacatat atcgacctgc tggagacaag gcgcacatac tatgagggac caggagaggg
     1921 ctctcccttc ggctggaagg acatcaagga gtggtacgag atgctgatgg gccactgcac
     1981 ctattttcca gaggagctga gaagcgtgaa gtacgcctat aacgccgatc tgtacaacgc
     2041 cctgaatgac ctgaacaacc tggtcatcac cagggatgag aacgagaagc tggagtacta
     2101 tgagaagttc cagatcatcg agaacgtgtt caagcagaag aagaagccta cactgaagca
     2161 gatcgccaag gagatcctgg tgaacgagga ggacatcaag ggctaccgcg tgacctccac
     2221 aggcaagcca gagttcacca atctgaaggt gtatcacgat atcaaggaca tcacagcccg
     2281 gaaggagatc atcgagaacg ccgagctgct ggatcagatc gccaagatcc tgaccatcta
     2341 tcagagctcc gaggacatcc aggaggagct gaccaacctg aatagcgagc tgacacagga
     2401 ggagatcgag cagatcagca atctgaaggg ctacaccggc acacacaacc tgagcctgaa
     2461 ggccatcaat ctgatcctgg atgagctgtg gcacacaaac gacaatcaga tcgccatctt
     2521 taaccggctg aagctggtgc caaagaaggt ggacctgtcc cagcagaagg agatcccaac
     2581 cacactggtg gacgatttca tcctgtctcc cgtggtgaag cggagcttca tccagagcat
     2641 caaagtgatc aacgccatca tcaagaagta cggcctgccc aatgatatca tcatcgagct
     2701 ggccagggag aagaactcca aggacgccca gaagatgatc aatgagatgc agaagaggaa
     2761 ccgccagacc aatgagcgga tcgaggagat catcagaacc acaggcaagg agaacgccaa
     2821 gtacctgatc gagaagatca agctgcacga tatgcaggag ggcaagtgtc tgtattctct
     2881 ggaggccatc cctctggagg acctgctgaa caatccattc aactacgagg tggatcacat
     2941 catcccccgg agcgtgagct tcgacaattc ttttaacaat aaggtgctgg tgaagcagga
     3001 ggagaacagc aagaagggca ataggacccc tttccagtac ctgtctagct ccgattctaa
     3061 gatcagctac gagacattca agaagcacat cctgaatctg gccaagggca agggccgcat
     3121 cagcaagacc aagaaggagt acctgctgga ggagcgggac atcaacagat tctccgtgca
     3181 gaaggacttc atcaaccgga atctggtgga caccagatac gccacacgcg gcctgatgaa
     3241 tctgctgcgg tcttatttca gagtgaacaa tctggatgtg aaggtgaaga gtatcaacgg
     3301 cggcttcacc tcctttctgc ggagaaagtg gaagtttaag aaggagcgca acaagggcta
     3361 taagcaccac gccgaggatg ccctgatcat cgccaatgcc gacttcatct ttaaggagtg
     3421 gaagaagctg gacaaggcca agaaagtgat ggagaaccag atgttcgagg agaagcaggc
     3481 cgagagcatg cccgagatcg agacagagca ggagtacaag gagattttca tcacacctca
     3541 ccagatcaag cacatcaagg acttcaagga ctacaagtat tctcacaggg tggataagaa
     3601 gcccaaccgc gagctgatca atgacaccct gtatagcaca cggaaggacg ataagggcaa
     3661 taccctgatc gtgaacaatc tgaacggcct gtacgacaag gataatgaca agctgaagaa
     3721 gctgatcaac aagtctcccg agaagctgct gatgtaccac cacgatcctc agacatatca
     3781 gaagctgaag ctgatcatgg agcagtacgg cgacgagaag aacccactgt ataagtacta
     3841 tgaggagaca ggcaactacc tgacaaagta tagcaagaag gataatggcc ccgtgatcaa
     3901 gaagatcaag tactatggca acaagctgaa tgcccacctg gacatcaccg acgattaccc
     3961 taactctcgc aataaggtgg tgaagctgag cctgaagcca taccggttcg acgtgtacct
     4021 ggacaacggc gtgtataagt ttgtgacagt gaagaatctg gatgtgatca agaaggagaa
     4081 ctactatgag gtgaacagca agtgctacga ggaggccaag aagctgaaga agatcagcaa
     4141 ccaggccgag ttcatcgcct ctttttacaa caatgacctg atcaagatca atggcgagct
     4201 gtatagagtg atcggcgtga acaatgatct gctgaacaga atcgaagtga atatgatcga
     4261 catcacctac agggagtatc tggagaacat gaatgataag aggccccctc gcatcatcaa
     4321 gaccatcgcc tctaagacac agagcatcaa gaagtacagc acagacatcc tggggaacct
     4381 gtatgaagtc aagagcaaga aacatcctca gattatcaag aaaggcccca agaagaagag
     4441 gaaggtgggt ggaggaggta ccggcggtgg aggctcagca gaatacgtac gagctctgtt
     4501 tgacttcaat gggaatgacg aggaggatct cccctttaag aagggcgata ttctccgcat
     4561 cagagataag cccgaagaac aatggtggaa tgccgaggat agcgaaggga aaaggggcat
     4621 gattctggtg ccatatgtgg agaaatattc cggtgactac aaagaccatg atggggatta
     4681 caaagaccac gacatcgact acaaagacga cgacgataaa tcagggatga cagacgccga
     4741 gtacgtgcgc attcatgaga aactggatat ttacaccttc aagaagcagt tcttcaacaa
     4801 caagaaatct gtgtcacacc gctgctacgt gctgtttgag ttgaagcgaa ggggcgaaag
     4861 aagggcttgc ttttggggct atgccgtcaa caagccccaa agtggcaccg agagaggaat
     4921 acacgctgag atattcagta tccgaaaggt ggaagagtat cttcgggata atcctgggca
     4981 gtttacgatc aactggtatt ccagctggag tccttgcgct gattgtgccg agaaaattct
     5041 ggaatggtat aatcaggaac ttcggggaaa cgggcacaca ttgaaaatct gggcctgcaa
     5101 gctgtactac gagaagaatg cccggaacca gataggactc tggaatctga gggacaatgg
     5161 tgtaggcctg aacgtgatgg tttccgagca ctatcagtgt tgtcggaaga ttttcatcca
     5221 aagctctcat aaccagctca atgaaaaccg ctggttggag aaaacactga aacgtgcgga
     5281 gaagcggaga tccgagctga gcatcatgat ccaggtcaag attctgcata ccactaagtc
     5341 tccagccgtt ggtcccaaga agaaaagaaa agtcggtacc atgaccaacc tttccgacat
     5401 catagagaag gaaacaggca aacagttggt catccaagag tcgatactca tgcttcctga
     5461 agaagttgag gaggtcattg ggaataagcc ggaaagtgac attctcgtac acactgcgta
     5521 tgatgagagc accgatgaga acgtgatgct gctcacgtca gatgccccag agtacaaacc
     5581 ctgggctctg gtgattcagg actctaatgg agagaacaag atcaagatgc tatagcttcg
     5641 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5701 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5761 atgtatctta gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     5821 taattgcgcg ccagcaaaag gccaagaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5881 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5941 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6001 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6061 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6121 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6181 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6241 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6301 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6361 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6421 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6481 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6541 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6601 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6661 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6721 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6781 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6841 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6901 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6961 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7021 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7081 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7141 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7201 cattctgaga atagtgtatg cggcgaccta gttgctcttg cccggcgtca atacgggata
     7261 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7321 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7381 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7441 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7501 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7561 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7621 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     7681 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     7741 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     7801 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     7861 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     7921 gagcccccga tttagagctt gacggggaaa gccgcagcta atggaccttc taggtcttga
     7981 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     8041 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     8101 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     8161 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     8221 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     8281 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
     8341 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     8401 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     8461 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     8521 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     8581 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     8641 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     8701 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     8761 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     8821 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     8881 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     8941 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     9001 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     9061 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     9121 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaattt
     9181 cgacccggat ccggccacca tggcgcgtaa ggtcgatctc acctcctgcg atcgcgagcc
     9241 gatccacatc cccggcagca ttcagtcgtg cggctgcctg ctagcctgcg acgcgcaggc
     9301 ggtgcggatc acgcgcatta cggaaaatgc cggcgcgttc tttggacgcg aaactccgcg
     9361 ggtcggtgag ctactcgccg attacttcgg cgagaccgaa gcccatgcgc tgcgcaacgc
     9421 actggcgcag tcctccgatc caaagcgacc ggcgctgatc ttcggttggc gcgacggcct
     9481 gaccggccgc accttcgaca tctcactgca tcgccatgac ggtacatcga tcatcgagtt
     9541 cgagcctgcg gcggccgaac aggccgacaa tccgctgcgg ctgacgcggc agatcatcgc
     9601 gcgcaccaaa gaactgaagt cgctcgaaga gatggccgca cgggtgccgc gctatctgca
     9661 ggcgatgctc ggctatcacc gcgtgatgtt gtaccgcttc gcggacgacg gctccgggat
     9721 ggtgatcggc gaggcgaagc gcagcgacct cgagagcttt ctcggtcagc actttccggc
     9781 gtcgctggtc ccgcagcagg cgcggctact gtacttgaag aacgcgatcc gcgtggtctc
     9841 ggattcgcgc ggcatcagca gccggatcgt gcccgagcac gacgcctccg gcgccgcgct
     9901 cgatctgtcg ttcgcgcacc tgcgcagcat ctcgccctgc catctcgaat ttctgcggaa
     9961 catgggcgtc agcgcctcga tgtcgctgtc gatcatcatt gacggcacgc tatggggatt
    10021 gatcatctgt catcattacg agccgcgtgc cgtgccgatg gcgcagcgcg tcgcggccga
    10081 aatgttcgcc gacttcttat cgctgcactt caccgccgcc caccaccaac gctaagcggc
    10141 cgcgactcta accggtctca gaagccatag agcccaccgc atccccagca tgcctgctat
    10201 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
    10261 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
    10321 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
    10381 aaggc
//
