LOCUS       sequence-378449-        8959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For C-terminus 3xGFP tagging of a gene of interest using URA3 
            selection marker in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Ball DA, Mehta GD, Salomon-Kent R, Mazza D, Morisaki T, Mueller F, 
            McNally JG, Karpova TS
  TITLE     Single molecule tracking of Ace1p in Saccharomyces cerevisiae 
            defines a characteristic residence time for non-specific 
            interactions of transcription factors with chromatin.
  JOURNAL   Nucleic Acids Res. 2016 Dec 1;44(21):e160. doi: 10.1093/nar/gkw744. 
            Epub 2016 Aug 26.
  PUBMED    27566148
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-12-1"; volume: "44(21):e160. doi"; pages: "
            10.1093/nar/gkw744. Epub 2016 Aug 26"
COMMENT     Sequence Label: pTSK405
FEATURES             Location/Qualifiers
     source          1..8959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1105..1818
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1825..2538
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2545..3261
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        3305..3521
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3522..4325
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(4587..4606)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4588..4606)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4613..4630)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4613..4629)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4622..4644)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4770..5225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4857..4876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5067..5088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5485..6147)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(6148..6335)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(6482..6501)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6601..6623
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6661..6679)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6747..6851
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6852..6920,6921..7712)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7070..7089)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7883..8471
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8372..8391
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8625..8642
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8759..8780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8795..8800,8801..8818,8819..8825)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8833..8849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8838..8860
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8857..8873
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8857..8873
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8894..8912
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     8940..8956
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8941..8957
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtagacgtat taaagctcaa tgatcctaca agacattaag taaaaagggg aagcgatctt
       61 aaatccacat gaatttcctt tctctttttc ttcttcttct gttccctttt tctttcttct
      121 tccttcaacg tctacgtaaa tataatgtat aacatctcca ctttcctttc ccttatagcg
      181 ttatgttacc tgtttaaact ctaaattctt catctactac tgctgttact acatgtatat
      241 atgttcattt tttttttttt ttttcaccac ggaaaacgta aaaatgagag catttgtaac
      301 aaataaagga gaactgttca atcaagtgac atacaaaaac tctctccaac tctacaatgg
      361 atcccgaata aacacacata aataaacaaa atggtcgtaa ttaacggggt caaatatgcc
      421 tgtgaaacgt gtatcagggg tcacagggcg gcgcagtgta ctcacactga tggtccgcta
      481 cagatgatca gacgcaaggg aagaccatcg accacatgtg gccattgtaa agagctgaga
      541 agaaccaaga acttcaaccc atccggtggg tgcatgtgtg cctctgcacg acggccagct
      601 gttggcagca aggaagatga aacacgatgt cgttgtgatg agggtgaacc ttgtaaatgt
      661 cataccaaga ggaaaagcag ccggaaatca aagggagggt catgccacag aagggcaaat
      721 gatgaagcag cgcatgtcaa tggtctcggt attgcagatc tggacgttct tttgggccta
      781 aatggtcgct cgtcggatgt agacatgaca accacattgc cgagtttgaa gccacctctg
      841 caaaacggag aaattaaggc cgacagcatt gacaatcttg atttggcttc cctcgatccg
      901 cttgagcaaa gccctagtat atctatggaa cctgttagta tcaatgaaac aggaagcgca
      961 tatacaacta cgaacacagc actaaacgat attgacattc cattctccat caatgagttg
     1021 aacgagctat acaaacaagt atcttcgcat aactcacatt cacaagcggc cgccgctgct
     1081 gccgctgcag ctgctgcagc tgcgatgtct aaaggtgaag aattattcac tggtgttgtc
     1141 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
     1201 ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa
     1261 ttgccagttc catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg
     1321 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     1381 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     1441 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     1501 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
     1561 atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa
     1621 gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     1681 gtcttgttac cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac
     1741 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     1801 atggatgaat tgtacaaaga attcatgtct aaaggtgaag aattattcac tggtgttgtc
     1861 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
     1921 ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa
     1981 ttgccagttc catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg
     2041 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     2101 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     2161 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     2221 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
     2281 atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa
     2341 gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     2401 gtcttgttac cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac
     2461 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     2521 atggatgaat tgtacaaatc tagaatgtct aaaggtgaag aattattcac tggtgttgtc
     2581 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
     2641 ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa
     2701 ttgccagttc catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg
     2761 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     2821 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     2881 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     2941 gatggtaaca ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc
     3001 atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa
     3061 gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     3121 gtcttgttac cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac
     3181 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     3241 atggatgaat tgtacaaata accgcggggt taatgtggct gtggtttcag ggtccataaa
     3301 gcttttcaat tcatcttttt tttttttgtt cttttttttg attccggttt ctttgaaatt
     3361 tttttgattc ggtaatctcc gagcagaagg aagaacgaag gaaggagcac agacttagat
     3421 tggtatatat acgcatatgt ggtgttgaag aaacgtgaaa ttgcccagta ttcttaaccc
     3481 aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata
     3541 aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg
     3601 aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt
     3661 tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg
     3721 atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt
     3781 tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg
     3841 cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc
     3901 caggtattgt tagcggtttg aagcaggcgg cggaagaagt aacaaaggaa cctagaggcc
     3961 ttttgatgtt agcagaattg tcatgcaagg gctccctagc tactggagaa tatactaagg
     4021 gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag
     4081 acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag
     4141 atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag
     4201 gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag
     4261 agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa
     4321 actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa
     4381 tttaattata tcagttatta cccgggaatc tcggtcgtaa tgatttctat aatgacgaaa
     4441 aaaaaaaaat tggaaagaaa aagcttcatg gcctttataa aaaggaacta tccaatacct
     4501 cgccagaacc aagtaacagt attttacggg gcacgaatca agaacaataa gacaggactg
     4561 taaagatgga cgcgagctcc aattcgccct atagtgagtc gtattacaat tcactggccg
     4621 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4681 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4741 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     4801 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4861 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4921 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4981 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5041 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5101 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5161 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     5221 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagat
     5281 ccgtcgagtt caagagaaaa aaaaagaaaa agcaaaaaga aaaaaggaaa gcgcgcctcg
     5341 ttcagaatga cacgtataga atgatgcatt accttgtcat cttcagtatc atactgttcg
     5401 tatacatact tactgacatt cataggtata catatataca catgtatata tatcgtatgc
     5461 tgcagcttta aataatcggt gtcactacat aagaacacct ttggtggagg gaacatcgtt
     5521 ggtaccattg ggcgaggtgg cttctcttat ggcaaccgca agagccttga acgcactctc
     5581 actacggtga tgatcattct tgcctcgcag acaatcaacg tggagggtaa ttctgctagc
     5641 ctctgcaaag ctttcaagaa aatgcgggat catctcgcaa gagagatctc ctactttctc
     5701 cctttgcaaa ccaagttcga caactgcgta cggcctgttc gaaagatcta ccaccgctct
     5761 ggaaagtgcc tcatccaaag gcgcaaatcc tgatccaaac ctttttactc cacgcacggc
     5821 ccctagggcc tctttaaaag cttgaccgag agcaatcccg cagtcttcag tggtgtgatg
     5881 gtcgtctatg tgtaagtcac caatgcactc aacgattagc gaccagccgg aatgcttggc
     5941 cagagcatgt atcatatggt ccagaaaccc tatacctgtg tggacgttaa tcacttgcga
     6001 ttgtgtggcc tgttctgcta ctgcttctgc ctctttttct gggaagatcg agtgctctat
     6061 cgctagggga ccacccttta aagagatcgc aatctgaatc ttggtttcat ttgtaatacg
     6121 ctttactagg gctttctgct ctgtcatctt tgccttcgtt tatcttgcct gctcattttt
     6181 tagtatattc ttcgaagaaa tcacattact ttatataatg tataattcat tatgtgataa
     6241 tgccaatcgc taagaaaaaa aaagagtcat ccgctaggtg gaaaaaaaaa aatgaaaatc
     6301 attaccgagg cataaaaaaa tatagagtgt actagaggag gccaagagta atagaaaaag
     6361 aaaattgcgg gaaaggactg tgttatgact tccctgacta atgccgtgtt caaacgatac
     6421 ctggcagtga ctcctagcgc tcaccaagct cttaaaacgg gaatttatgg tgcactctca
     6481 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     6541 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6601 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     6661 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6841 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6901 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6961 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7021 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7081 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7141 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7201 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7261 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7321 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7381 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7441 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7501 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7561 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7621 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7681 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7741 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7801 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7861 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7921 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7981 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8041 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8101 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8161 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8221 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8281 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8341 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8401 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8461 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8521 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8581 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8641 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8701 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8761 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8821 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     8881 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtaccg ggccccccct
     8941 cgaggtcgac ggtatcgat
//
